Citrus Fruit Juices Research Articles

Green and facile method for the recovery of spent Lithium Nickel Manganese Cobalt Oxide (NMC) based Lithium ion batteries

The research reports a novel green method to use citrus fruits for the management of spent NMC based lithium ion batteries (LIBs). Citrus fruit juice (CJ) can provide an excellent chemical combination to remove the binder and support the leaching with efficiency in between 94% to 100%. CJ have many advantages in LIBs recycling as an economic and green method due to rich in many organic acids like citric and malic acid as complexing agents with ascorbic acid and citrus flavonoids, for the reduction of many heavy metals. Application of CJ can avoid the use of N-Methylpyrrolidine, γ-Butyrolactone, dimethylformamide, and dimethyl sulfoxide like toxic solvents commonly used for peeling off Al/Cu. Furthermore, counterions (like Na+, Mg+, Ca2+) present in CJ was responsible for the improvement in the leaching efficiency of organic acids. A mechanistic pattern of the overall reaction was also proposed and duly supported by various spectroscopic techniques. Binder removal experiment was supported by analytical techniques like XRD, XRF, IR, and FE-SEM, while the metal concentration was monitored by using ICP-MS analysis.

Effects of Lemon and Seville Orange Juices on the Pharmacokinetic Properties of Sildenafil in Healthy Subjects.

PurposeSeveral severe drug interactions have been reported when sildenafil, a potent drug for the treatment of erectile dysfunction, is co-administered with drugs or herbal remedies that inhibit cytochrome P450 (CYP) 3A4. This study evaluates the effects of two citrus fruit juices, lemon and Seville orange, on the pharmacokinetics of sildenafil in male healthy subjects following a single oral dose.MethodsWe conducted an open-label, three-way crossover study in nine healthy male volunteers. Participants received a single oral dose of sildenafil (50 mg) after pretreatment with 250 mL of either water (control), undiluted lemon juice, or Seville orange juice for 3 consecutive days. All subjects were monitored for adverse effects during the study period. Plasma samples were collected for 12 h after dosing and analyzed for sildenafil concentration.ResultsCompared with pretreatment with water, Seville orange juice significantly increased the area under the plasma concentration-time curve from time zero to infinity and the peak plasma concentration of sildenafil by 44 % (90 % confidence interval [CI] 30–60) and 18 % (90 % CI 108–129), respectively, without affecting the time to reach peak plasma concentration. Additionally, Seville orange juice significantly reduced the apparent oral clearance of sildenafil by 30 % (90 % CI 63–75) without affecting its elimination half-life. In contrast, lemon juice did not cause any significant alterations in the pharmacokinetics of sildenafil. There was no significant treatment-related adverse effects reported during the study.ConclusionsAlthough it is considered as a moderate CYP3A4 inhibitor, Seville orange only caused a mild increase in exposure to sildenafil after a single oral dose, without manifestation of any adverse effects. The enhanced bioavailability of sildenafil by Seville orange may be attributed to inhibition of its intestinal first-pass effect (CYP3A4 and or p-glycoprotein). Lemon juice, in contrast, had no effects on the pharmacokinetics of sildenafil.

Extraction of Citric Acid from Fruit Juices using Supported Liquid Membrane

This study deals with facilitated transport of citric acid using supported liquid membrane composed of polytetrafluroethylene membrane as the support, Aliquat-336, toluene, sodium carbonate + sodium bicarbonate as carrier, organic-membrane and strip phase, respectively, from aqueous solutions and fruit juices. The optimum citric acid extraction conditions from aqueous solutions in batch mode were investigated and the standardized conditions were applied for extraction of citric acid from fruit juices. Complete and partial (33%) citric acid extraction was achieved from sweet-lime, lemon, and orange juices with sodium carbonate + sodium bicarbonate and distilled water as strip solutions, respectively. Practical Applications The present study of facilitated and controlled extraction of citric acid from citrus fruit juices using supported liquid membrane stands as an alternative method of de-souring citrus fruit juices for improving the palatability and consumer acceptability of the processed citrus fruit juice. The standardized various extraction parameters provide optimum conditions for complete or partial citric acid from model systems and fruit juices.

Microbiological and physicochemical properties of commercial seal tampered refrigerated fruit juices

<p>Microbial and physicochemical properties of seven branded, seal tampered refrigerated fruit juices were carried out in this study using standard methods. Coliform counts ranged from 2.079 to3.093 log<sub>10</sub>cfu/ml over the storage period with pineapple juice and citrus juice having the highest and least coliform count respectively. Total bacteria count in the juice ranged from 7.009 to 8.243 log<sub>10</sub>cfu/ml. Citrus fruit juice however had the highest staphylococcal count while pineapple juice had the least (2.344 to 3.881log<sub>10</sub>cfu/ml). Also, osmophilic yeast count ranged from 2.017 to 3.903log<sub>10</sub>cfu/ml, having the highest load in orange fruit juice and lowest load in citrus fruit juice. The pH of the juice samples ranged from 2.9 to 4.2 during the period of refrigeration. Conductivity was highest in apple fruit juice and lowest in orange nectar pulp fruit juice. The total dissolved solids ranged from 0.29 to 1.95 over storage and was recorded highest in apple juice and lowest in orange nectar pulp fruit juice. Turbidity ranged from 5.8-200. These results indicate a reduction in the quality of fruit juices after 5 days of opening and thus reveals that both spoilage and pathogenic organisms could proliferate in juices despite refrigeration.</p>

Organic acid and sugar contents in different domestic fruit juices

Organic acid and sugar contents in ten kinds of juices (two orange juices, two grape juices, two aloe juices, two citron juices, one bokbunja, and one grapefruit juice) were analyzed by high-performance liquid chromatography. Citric acid was detected in all the fruit juices analyzed. Grape juices contained the highest tartaric acid content (67.85 - 99.37 mg/100 mL) while orange and grape juices contained a high content of malic acid (151.67 - 211.18 mg/100 mL). Lactic acid was detected in all the aloe juices (35.12 - 65.27 mg/100 mL) as well as in one orange (203.8 mg/100 mL) and one grape juice (112.28 mg/100 mL). Citrus fruit juices (A, A’, F) showed the highest content of total organic acids as 902.81 - 1,103.7 mg/100 mL. With regard to sugar contained in the juices, lactose and maltose were not detected in any of the fruit juices. Even when comparing the same kind of fruit juice, sugar (fructose, glucose, and sucrose) content showed a significant difference (p < 0.05) depending on the manufacturer. In the grape juices, contents of fructose (6.86 - 7.51) and glucose (6.23 - 7.3 g/100 mL) were higher than in other juices. One serving size of the juices (180 mL) analyzed in this study can provide approximately 3.3 - 4.9% of total daily energy requirements when consumed as part of the 2,200 kcal/day diet required for an adult.

Pasteurization of citrus juices with ohmic heating to preserve the carotenoid profile

This study was carried out to assess, for the first time, the effect of ohmic heating on the carotenoid profile of two citrus fruit juices: grapefruit and blood orange. Two heat treatments were designed to obtain pasteurization values of 50 and 150 min (Tref= 70°C and z-value=10°C) with ohmic heating as compared to conventional heating. The results showed that xanthophyll losses could reach 70% for epoxyxanthophylls (cis-violaxanthin and cis-antheraxanthin) and 40% for hydroxyxanthophylls (β-cryptoxanthin, lutein, and zeaxanthin) with conventional heating, but losses were under 30% and 20%, respectively, with ohmic heating. Carotene species (lycopene and β-carotene) were stable regardless of the treatment. No negative nonthermal effects of ohmic heating were shown on carotenoids. Loss simulations of the studied carotenoids showed that the high temperatures reached with ohmic heating during pasteurization could substantially increase the organoleptic and nutritional quality of acid carotenoid-rich juices.

Upscale of recombinant α-L-rhamnosidase production by Pichia pastoris Mut(S) strain.

Pichia pastoris is currently one of the most preferred microorganisms for recombinant enzyme production due to its efficient expression system. The advantages include the production of high amounts of recombinant proteins containing the appropriate posttranslational modifications and easy cultivation conditions. α-L-Rhamnosidase is a biotechnologically important enzyme in food and pharmaceutical industry, used for example in debittering of citrus fruit juices, rhamnose pruning from naringin, or enhancement of wine aromas, creating a demand for the production of an active and stable enzyme. The production of recombinant α-L-rhamnosidase cloned in the MutS strain of P. pastoris KM71H was optimized. The encoding gene is located under the control of the AOX promoter, which is induced by methanol whose concentration is instrumental for these strain types. Fermentation was upscaled in bioreactors employing various media and several methanol-feeding strategies. It was found that fed batch with BSM media was more effective compared to BMMH (Buffered Methanol-complex Medium) media due to lower cost and improved biomass formation. In BSM (Basal Salt Medium) medium, the dry cell weight reached approximately 60 g/L, while in BMMH it was only 8.3 g/L, without additional glycerol, which positively influenced the amount of enzyme produced. New methanol feeding strategy, based on the level of dissolved oxygen was developed in this study. This protocol that is entirely independent on methanol monitoring was up scaled to a 19.5-L fermenter with 10-L working volume with the productivity of 13.34 mgprot/L/h and specific activity of α-L-rhamnosidase of 82 U/mg. The simplified fermentation protocol was developed for easy and effective fermentation of P. pastoris MutS based on dissolved oxygen monitoring in the induction phase of an enzyme production.

Gluconacetobacter sp. gel_SEA623-2, bacterial cellulose producing bacterium isolated from citrus fruit juice.

Cellulose producing bacterial strain was isolated from citrus fruit juice fungus. The isolated strain was identified as Gluconacetobacter sp. gel_SEA623-2 based on several morphological characteristics, biochemical tests, and 16S rRNA conducted. Culture conditions for bacterial cellulose production by SEA623-2 were screened in static trays. Conditions were extensively optimized by varying the kind of fruit juice, pH, sugar concentration, and temperature for maximum cellulose production. SEA623-2 has a high productive capacity in citrus processing medium, but not in other fruits. The optimal combination of the media constituents for bacterial cellulose production is as follows: 10% citrus juice, 10% sucrose, 1% acetic acid, and 1% ethanol at 30 °C, pH 3.5. Bacterial cellulose produced by SEA623-2 has soft physical properties, high tensile strength, and high water retention value. The cellulose produced by the selected bacteria is suitable as a cosmetic and medical material.

Drinking citrus fruit juice inhibits vascular remodeling in cuff-induced vascular injury mouse model.

Citrus fruits are thought to have inhibitory effects on oxidative stress, thereby attenuating the onset and progression of cancer and cardiovascular disease; however, there are few reports assessing their effect on vascular remodeling. Here, we investigated the effect of drinking the juice of two different citrus fruits on vascular neointima formation using a cuff-induced vascular injury mouse model. Male C57BL6 mice were divided into five groups as follows: 1) Control (water) (C), 2) 10% Citrus unshiu (CU) juice (CU10), 3) 40% CU juice (CU40), 4) 10% Citrus iyo (CI) juice (CI10), and 5) 40% CI juice (CI40). After drinking them for 2 weeks from 8 weeks of age, cuff injury was induced by polyethylene cuff placement around the femoral artery. Neointima formation was significantly attenuated in CU40, CI10 and CI40 compared with C; however, no remarkable preventive effect was observed in CU10. The increases in levels of various inflammatory markers including cytokines such as monocyte chemotactic protein-1, interleukin-6 (IL-6), IL-1β, and tumor necrosis factor-α in response to vascular injury did not differ significantly between C, CU10 and CI10. The increases in cell proliferation and superoxide anion production were markedly attenuated in CI10, but not in CU10 compared with C. The increase in phosphorylated ERK expression was markedly attenuated both in CU10 and CI10 without significant difference between CU10 and CI10. Accumulation of immune cells did not differ between CU10 and CI10. These results indicate that drinking citrus fruit juice attenuates vascular remodeling partly via a reduction of oxidative stress. Interestingly, the preventive efficacy on neointima formation was stronger in CI than in CU at least in part due to more prominent inhibitory effects on oxidative stress by CI.

IDENTIFIKASI POTENSI SARI BUAH JERUK MENJADI LISTRIK SEBAGAI SUMBER BELAJAR FISIKA MATERI ARUS LISTRIK SISWA SMP KELAS IX

The purpose of this study was 1. To know potential of different citrus fruit juice varieties into electricity. 2. To know juice of citrus varieties that have the potential to be superior in electrical 3. To know piece of research that can be used as a learning resource. 4. To know learning resources that can be generated from the research. The place is a laboratory study was conducted of science, University of Muhammadiyah Metro May 25, 2014. Oranges juice were taken from five different varieties are citrus, lime, tangerine, grapefruit, and lime. There are three variations of existing experiments in the study of differences in varieties, differences in the distance between the electrodes, and the difference in volume. There are five treatments and five repetitions in this experiment. In the process the research data used ANOVA analysis of non-parametric Kruskal Wallis test. The experimental results showed that the juice that comes from different varieties have different potentials in into electricity. Lemon juice is the most superior varieties as electricity. Part of research that can be used as a learning resource that the pH value of each citrus varieties, the use of digital multitester, and the voltage generated. The results of this study can benefit as a learning resource in the form of worksheets.

Polyphenolic contents in Citrus fruit juices: authenticity assessment

The contents of 49 polyphenols in sweet orange, tangerine, lemon and grapefruit juices from 18 cultivars grown in Spain were determined by reversed-phase high-performance liquid chromatography with photodiode array detection. Citrus polyphenolic profiles consist of 81–97 % of flavanones, 0.3–13.6 % of flavones, 0.1–6.0 % of flavonols, 0.6–9.6 % of hydroxycinnamic acids and 0.2–0.4 % of coumarins (only found in grapefruit juices). Several markers that allow to distinguish with practical certainty grapefruit and lemon juices between them and from the other Citrus species are reported. Each of these markers is a reliable and useful tool to detect juice adulteration. Grapefruit juice markers were naringenin-7-O-neohesperidoside, naringenin-7-O-neohesperidoside-4′-O-glucose, naringenin-O-hexosylhexoside, hesperetin-7-O-neohesperidoside, naringenin-O-rhamnosylmalonylhexoside, isosakuranetin-7-O-neohesperidoside, hesperetin-7-O-rutinoside, apigenin-6-C-hexoside-O-hexoside, apigenin-7-O-neohesperidoside and scopoletin-O-hexoside. Lemon juice markers were eriodictyol-7-O-rutinoside-4′-O-glucoside, eriodictyol-7-O-rutinoside, diosmetin-6,8-di-C-glucoside, diosmetin-8-C-glucoside, luteolin-7-O-rutinoside, diosmetin-6-C-glucoside and diosmetin-6,8-di-C-hexosideacylhexoside. The markers naringenin-O-hexosylhexoside, apigenin-6-C-hexoside-O-hexoside, scopoletin-O-hexoside, diosmetin-8-C-glucoside and diosmetin-6,8-di-C-hexosideacylhexoside were detected, characterized and quantitatively determined in grapefruit and lemon juices for the first time by our research group, as far as the authors know. Classification models provided by LDA and PLS-DA correctly identify all sweet orange and tangerine juices. Moreover, PLS regression model determines the percentage (10–70 %) of tangerine juice used to adulterate sweet orange juice with a suitable confidence interval (RMSEP = 7 %).

Optimization of the Production of α-L-Rhamnosidase by Aspergillus niger in Solid State Fermentation Using Agro-Industrial Residues

ABSTRACT The enzyme α-L-rhamnosidase specifically cleaves terminal rhamnose residues from awide variety of natural products. This property endows this enzyme with importantbiotechnological potential as L-rhamnosidase could be employed in a variety ofapplications, including removing bitterness from citrus fruit juices, improving the aroma ofwines and converting clinically important steroids. This work optimized α-L-rhamnosidasesolid-state fermentation production from Aspergillus niger 426 using statistical methods.Firstly, a statistical mixture-design with three components to determine the best ratio ofnutrients for enzyme production was carried out. The optimal conditions consisted ofgrowing the fungi in media containing 0.14 g of cane sugar bagasse, 1.25 g of soybeanhulls and 3.05 g of rice straw; these conditions achieved a maximum α-L-rhamnosidaseactivity of 1.92 U / mL. Next, a 3² Box-Behnken design to optimize culture moisture levelsand nutrient solution pH values for enzyme production was carried out. α-L-rhamnosidaseactivity increased to 3.02 U / mL when medium moisture was 75.5% and pH value of4.0.Original Research Article

Role of pomegranate and citrus fruit juices in colon cancer prevention.

Colorectal cancer is the second leading cause of cancer-related deaths in the United States. Recent studies prove that though chemotherapeutic agents are being used for the treatment of colon cancer, they become non-effective when the cancer progresses to an invasive stage. Since consumption of certain dietary agents has been linked with various cancers, fruit juices have been investigated for their consistently protective effect against colon cancer. The unique biochemical composition of fruit juices is responsible for their anticancer properties. In this review, the chemo-preventive effect of fruit juices such as pomegranate and citrus juices against colon cancer are discussed. For this purpose, the bioavailability, in vitro and in vivo effects of these fruit juices on colorectal cancer are highlighted. Moreover, there is a scarcity of studies involving human trials to estimate the preventive nature of these juices against colon cancer. This review will support the need for more preclinical tests with these crude juices and their constituents in different colorectal cancer cell lines and also some epidemiological studies in order to have a better understanding and promote pomegranate and citrus juices as crusaders against colon cancer.

Effect of Shaddock Citrus Fruit Juice on the Lipid Profile, Fasting Blood Glucose Level and Kidney Function of Male Wistar Albino Rats

Effect of Shaddock Citrus Fruit Juice on the Lipid Profile, Fasting Blood Glucose Level and Kidney Function of Male Wistar Albino Rats

Optimization of Process Parameters by Statistical Experimental Designs for the Production of Naringinase Enzyme by Marine Fungi

Naringinase has attracted a great deal of attention in recent years due to its hydrolytic activities which include the production of rhamnose and prunin and debittering of citrus fruit juices. Screening of fifteen marine-derived fungi, locally isolated from Ismalia, Egypt, for naringinase enzyme production, indicated thatAspergillus nigerwas the most promising. In solid state fermentation (SSF) of the agroindustrial waste, orange rind was used as a substrate containing naringin. Sequential optimization strategy, based on statistical experimental designs, was employed to enhance the production of the debittering naringinase enzyme. Effects of 19 variables were examined for their significance on naringinase production using Plackett-Burman factorial design. Significant parameters were further investigated using Taguchi’s (L1645) orthogonal array design. Based on statistical analysis (ANOVA), the optimal combinations of the major constituents of media for maximal naringinase production were evaluated as follows: 15 g orange rind waste, 30 mL moisture content, 1% grape fruit, 1% NaNO3, 0.5% KH2PO4, 5 mM MgSO4, 5 mM FeSO4, and the initial pH 7.5. The activity obtained was more than 3.14-fold the basal production medium.

Stachydrine ameliorates high-glucose induced endothelial cell senescence and SIRT1 downregulation

Hyperglycaemia, a characteristic feature of diabetes mellitus, induces endothelial dysfunction and vascular complications by accelerating endothelial cell (EC) senescence and limiting the proliferative potential of these cells. Here we aimed to investigate the effect of stachydrine, a proline betaine present in considerable quantities in juices from fruits of the Citrus genus, on EC under high-glucose stimulation, and its underlying mechanism. The senescence model of EC was set up by treating cells with high-glucose (30 mM) for different times. Dose-dependent (0.001-1 mM) evaluation of cell viability revealed that stachydrine does not affect cell proliferation with a similar trend up to 72 h. Noticeable, stachydrine (0.1 mM) significantly attenuated the high-glucose induced EC growth arrest and senescence. Indeed, co-treatment with high-glucose and stachydrine for 48 h kept the percentage of EC in the G0 /G1 cell cycle phase near to control values and significantly reduced cell senescence. Western blot analysis and confocal-laser scanning microscopy revealed that stachydrine also blocked the high-glucose induced upregulation of p16(INK4A) and downregulation of SIRT1 expression and enzyme activity. Taken together, results here presented are the first evidence that stachydrine, a naturally occurring compound abundant in citrus fruit juices, inhibits the deleterious effect of high-glucose on EC and acts through the modulation of SIRT1 pathway. These results may open new prospective in the identification of stachydrine as an important component of healthier eating patterns in prevention of cardiovascular diseases.

Ultra high performance liquid chromatography with ion‐trap TOF‐MS for the fast characterization of flavonoids in Citrus bergamia juice

We have developed a fast ultra HPLC with ion-trap TOF-MS method for the analysis of flavonoids in Citrus bergamia juice. With respect to the typical methods for the analysis of these matrices based on conventional HPLC techniques, a tenfold faster separation was attained. The use of a core-shell particle column ensured high resolution within the fast analysis time of only 5 min. Unambiguous determination of flavonoid identity was obtained by the employment of a hybrid ion-trap TOF mass spectrometer with high mass accuracy (average error 1.69 ppm). The system showed good retention time and peak area repeatability, with maximum RSD% values of 0.36 and 3.86, respectively, as well as good linearity (R(2) ≥ 0.99). Our results show that ultra HPLC can be a useful tool for ultra fast qualitative/quantitative analysis of flavonoid compounds in citrus fruit juices.

Simplified miniaturized ultrasound-assisted matrix solid phase dispersion extraction and high performance liquid chromatographic determination of seven flavonoids in citrus fruit juice and human fluid samples: Hesperetin and naringenin as biomarkers

In the present study, for the first time, a simplified miniaturized ultrasound-assisted matrix solid-phase dispersion (SM-USA-MSPD) method with a different application for liquid matrices was developed to extract different flavonoids (hesperidin, diosmin, eriocitrin, narirutin, naringin, hesperetin and naringenin) from citrus fruit juice and human fluid samples prior to their determination using high performance liquid chromatography (HPLC). Different effective parameters were studied and under the optimum conditions (including sample volume: 150μL; solid phase: silica-based C18, 200mg; eluting solvent: methanol, 500μL; pH: 4; and sonication: 6min; at room temperature), limits of detection and limits of quantification were ranged from 23.3 to 46.8ngmL−1 and 74.8 to 141.5ngmL−1, respectively. Once optimized, analytical performance of the method was studied in terms of linearity (0.074–198.5μgmL−1, r2>0.991), accuracy (recovery=84.6–101.5%), and precision (repeatability: intra-day precision<5.9%, and inter-day precision<7.2%). At the end, SM-USA-MSPD method was successfully applied to estimate the levels of hesperetin and naringenin in plasma and urinary excretion -after ingestion of orange, grapefruit and lime juices- and the obtained results confirmed that these compounds could be used as good biomarkers of citrus fruit juice intake.

Effect of coagulant protein from Parkinsonia aculeata seed and Citrus juice on bacteria isolated from Ruvu River in Tanzania

A total of 126 bacterial strains were isolated from water samples collected from Ruvu River in Tanzania. Seventy strains were identified by morphological, biochemical and 16s rRNA gene sequence analysis. The strains were found to belong to Acinetobacter, Pseudomonas, Vibrio, Aeromonas or Bacillus spp. Coagulant protein was purified from Parkinsonia aculeata seed by ion exchange chromatography. Antibacterial effect of the purified P. aculeata coagulant protein (PAP) and Citrus fruit juice (CF) was tested on four bacterial strains that belonged to different genera. Bacterial cell flocculation analyses showed that, PAP and CF aggregate both Gram-positive and Gram-negative bacteria. Growth studies of bacterial strains incubated with PAP and CF revealed that, inhibitory dosages of PAP ranged from 0.38 mg/L to 0.88 mg/L and growth inhibition occurred within two to four hours. The PACE and PAP showed high coagulation activities that were similar to those observed for chemical coagulant (alum) with turbidity removal efficiencies ranging from 93 to 98%. A simplified method used for purification of proteins together with high antimicrobial and water coagulation activity make proteins suitable for supplementing chemical coagulants in drinking water treatment. Also, CF is suitable for post disinfection of water treated with proteins prior drinking in poor communities.

On line characterization of 58 phenolic compounds in Citrus fruit juices from Spanish cultivars by high-performance liquid chromatography with photodiode-array detection coupled to electrospray ionization triple quadrupole mass spectrometry

Polyphenol profile of Citrus juices of sweet orange, tangerine, lemon and grapefruit from Spanish cultivars was obtained by High-Performance Liquid Chromatography with Diode Array Detection coupled to Electrospray ionization and Triple Quadrupole Mass Spectrometry. Fifty eight phenolic compounds of five different classes were identified in these Citrus juices. Flavanone: O-dihexoside of naringenin; flavones: apigenin-7-O-rutinoside-4′-O-glucoside, luteolin-7-O-neohesperidoside-4′-O-glucoside, luteolin-6-C-glucoside, 6,8-di-C-acylhexosides of chrysoeriol and diosmetin, 6C- and 8C-glucoside-O-pentoside of apigenin, apigenin-6-C-hexoside-O-hexoside and apigenin-8-C-hexoside-O-acylrhamnoside; flavonols: 7-O-rutinosides of quercetin, kaempferol, isorhamnetin and tamarixetin, kaempferol-3-O-rutinoside, isorhamnetin-3-O-rutinoside-7-O-glucoside, tamarixetin-3-O-rutinoside-7-O-glucoside, isorhamnetin-3-O-hexoside-7-O-rhamnosylhexoside, 3-O-rhamnoside-7-O-rhamnosylhexoside of quercetin and isorhamnetin and kaempferol-3-O-rhamnosylhexoside-7-O-rhamnoside; hydroxycinnamic acids: O-hexoside of ferulic and sinapic acid; and, coumarins: O-hexoside and O-rhamnosylhexoside of scopoletin, had not previously been reported in Citrus juices to our knowledge. Structures have been assigned on the basis of the complementary information obtained from retention time, UV–visible spectra, scan mode MS spectra, and fragmentation patterns in MS2 spectra obtained using different collision energies. A structure diagnosis scheme is provided for the identification of different phenolic compounds.