Citrus Aurantifolia Research Articles

In ovo feeding of Citrus aurantiifolia seed extract on hatchability, chick quality, physiological response, intestinal morphology and posthatch growth performance of cobb 500 broiler chickens

In ovo feeding of Citrus aurantiifolia seed extract on hatchability, chick quality, physiological response, intestinal morphology and posthatch growth performance of cobb 500 broiler chickens

Fruit and Seed Development of Citrus aurantifolia (Christm.) Swingle ‘Pan Rampai’ Cultivar

The ‘Pan Rampai’ cultivar of Mexican lime is highly consumed for its lime aroma and high juice content. However, occasional instances of low juice content could be found, potentially due to premature harvesting. Moreover, the seed development of the ‘Pan Rampai’ lime has never been investigated. Hence, this study was aimed to explore the development process of the ‘Pan Rampai’ lime fruit, focusing on its fruit weight, juice weight, juice content, fruit diameter, fruit length, fruit rind thickness, total soluble solids, titratable acid, TSS/TA ratio, and vitamin C content over the period of 11 to 18 weeks after flowering (WAF). Additionally, we examined the seed development by observing seed width, length, thickness, and germination percentage. Our findings revealed that fruit weight, juice weight, juice content, fruit diameter, and fruit length exhibited a consistent increase throughout the fruit development stages, while the fruit rind thickness decreased. Seed width and thickness remained relatively stable over 11 to 18 WAF, whereas seed length gradually increased. The study of germination percentage indicated that seed germination was mature from the 15th WAF, coinciding with the appearance of the secondary wall of seed coat. There results provide crucial insights into the optimal harvesting periods for juice (from 17 WAF) and seed (from 15 WAF) for the ‘Pan Rampai’ lime cultivar.

Machine learning-assisted optimization and evaluation of methylene blue adsorption kinetics on citrus aurantifolia leaves: Insights from isotherm and thermodynamic studies

BackgroundMethylene Blue (MB) is a cationic dye widely used in various industrial and pharmacological applications. However, its improper disposal poses significant environmental and health risks due to its toxicity, carcinogenicity, and resistance to biodegradation. Despite extensive efforts have been done to remove MB using green synthesis approaches, however; there remains a critical need to enhance the efficacy of these methods through proper optimization and the use of novel green sources. Addressing these challenges is essential to mitigate the environmental impact of MB. MethodsCitrus aurantifolia leaves powder (CALP), a simple, environmentally friendly, adsorbent has been utilized to remove MB dye. The adsorption kinetic efficacy of CALP was studied under different experimental conditions, including pH (3–9), contact time (2–8 min), and MB dye concentrations (10–70 mg/L). Additionally, machine learning (ML) was employed to optimize experimental conditions, predict, and validate the adsorption kinetic efficacy. The machine learning (ML) optimized experimental dataset which included parameters of pH (9), contact time (2 min), CALP adsorbent (0.3 gm), and MB dye concentration (70 mg/L) has shown maximum adsorption efficacy. To enhance and assess the extraction efficiency of MB dye, a response surface methodology (RSM) was employed utilizing a central composite design (CCD). Significant findingsThe adsorption efficacy of CALP was verified by the Freundlich isotherm model, which demonstrated an adsorption capacity of 415.05 mg/g with a coefficient of determination R2 of 0.999. The nonlinear pseudo-second-order kinetic model with an R2 value of 0.998 further confirms MB dye adsorption. In addition, spontaneous nature of the process was determined by calculating thermodynamic parameters such as ΔG°, ΔH°, and ΔS°. The outcomes demonstrate that CALP is a highly effective, reliable, and easily available adsorbent for the removal of MB dyes from wastewater. To the best of the author's knowledge, there are substantial unmet research gaps in the fields of wastewater treatment and adsorption modeling optimization. In particular, research that combine cutting-edge machine learning methods with conventional adsorption models to enhance process efficiency and prediction accuracy are scarce. This study is the first to employ CALP, a cost-effective and environmentally sustainable natural adsorbent, for the adsorption of MB dye utilizing a machine learning-optimized approach.

Effect of IBA and growing media on growth and survival of stem cutting in Kagzi Lime (Citrus aurantifolia)

Effect of IBA and growing media on growth and survival of stem cutting in Kagzi Lime (Citrus aurantifolia)

A combination of Citrus aurantifolia fruit rind and Theobroma cacao seed extracts supplementation enhances metabolic rates in overweight subjects: a randomized, placebo-controlled, cross-over study.

LN19183 is a proprietary, synergistic combination of Citrus aurantifolia fruit rind and Theobroma cacao seed extracts that increased resting energy expenditure (REE) in high-fat diet (HFD)-fed obese rats. The objective of this study was to validate the thermogenic potential of LN19183 in obese Sprague Dawley (SD) rats and to assess its clinical efficacy in a proof-of-concept, randomized, placebo-controlled, cross-over human trial. In the rat study, HFD-fed obese rats were supplemented with either HFD alone or with 45, 90, or 180 mg LN19183 per kg body weight (BW) for 28 days. In the human study, 60 overweight adults (male and female, aged 20-39 years) were randomized. Subjects took LN19183 (450 mg) or a matched placebo capsule on two consecutive days in phases one and two of the study, separated by a 10-day washout period. In each phase, on day 1, REE at pre-dose, 60-, 120-, and 180-min post-dose, and on day 2, metabolic rates at pre-dose and post-dose during and 20 min after exercise were measured using indirect calorimetry. In rats, LN19183 significantly increased REE, reduced BW gain and fat masses, and increased fat and carbohydrate metabolism marker proteins including beta 3 adrenergic receptor (β3-AR), phospho-AMP-activated protein kinase (AMPK), glucagon-like peptide-1 receptor (GLP-1R) in the liver, and serum adiponectin levels. Furthermore, LN19183-supplemented human volunteers increased (P < 0.05, vs. placebo) the metabolic rates at rest and with exercise; their fat oxidation was increased (P < 0.05, vs. placebo) at rest and 20 min post-exercise. The groups' systolic and diastolic blood pressure (BP), heart rates (HR), and safety parameters were comparable. These observations suggest that LN19183 is a thermogenic botanical composition with no stimulatory effects on BP and HR.

Health impacts of some fungal communities found on the surface of selected citrus fruits from Bwari market Abuja, Nigeria

Fruits are in great demand since they are nutrient-dense. For this reason, it becomes highly essential to create more in the shortest amount of time in order to keep up with the always-rising demand. As a result, there has been a significant chance that these fruits may become contaminated with harmful microbes, raising concerns about consumer safety. This study sought to ascertain the potential health effects of fruit contamination by fungal species found on the fruit surfaces, as there is currently insufficient information in this area. Three (3) species of Citrus (CF) which include; lemon (Citrus limon (L.) Burm. f.), Chinese orange or sweet orange (Citrus sinensis (L.) Osbeck), and key lime or lime (Citrus aurantifolia (Christm.) Swingle) were randomly bought from different hawkers at different parts of Bwari market in Abuja, Nigeria. From the studies, five different fungal species were identified and they include: Penicillium spp Trichophyton spp, Aspergillus spp, Candida spp and Rhizopus spp. This demonstrated that fungi exist in all environments and that handling fruits improperly can have serious negative health impacts on people since some of these fungi are pathogenic. A number of approaches, such as washing fruits in vinegar water, have been found to be very effective in reducing microbial infection. Other washing methods, such as using saltwater, also work well. Additionally, it is a good idea to regularly replace the water used to wash those fruits in order to prevent cross-contamination while handling.

Quality improvement of lime fruit (Citrus aurantifolia) between packaged and unpackaged conditions combined with different storage temperatures

Background: The use of limes is increasingly widespread worldwide, both as fresh fruit for consumption and for making juice or other drinks. Lime is also used in making jams and candies. The essential oil obtained from the skin is widely used in the pharmaceutical and cosmetic industries for medicines, perfumes, soaps, body lotions, and detergents. However, during fruit storage, the respiration and metabolic activity in lime fruits is directly related to the increase in ambient temperature, which makes the fruit dry out. Improving the shelf life of lime against post-harvest stress while maintaining the sensory and nutritional qualities of fresh produce can be achieved through low-temperature storage and the use of packaging. This study aims to improve the quality of lime by knowing the difference between the provision of packaging and not-given packaging combined with different storage temperatures. Method: The experimental design used a single-factor completely randomized design with storage type treatment, which includes room temperature without packaging (control), room temperature with packaging, low temperature without packaging, and low temperature with packaging. Observations of non-destructive characteristics include weight loss, respiration rate, and fruit peel color. Observations of destructive characteristics include juice content, fruit peel softness, Soluble Solid Contents (SSC), Total Acidity (TA), SSC/TA ratio, and ascorbic acid content. Finding: Low temperature treatment without packaging provides the best results in delaying weight loss, respiration rate, and SSC/TA ratio. The control treatment provided the best results for peel softness, juice content, and ascorbic acid. Conclusion: Low temperature treatment without packaging is recommended for the storage of lime fruit to extend its shelf life. Novelty/Originality of this Study: This study examines the combined effects of low temperature and newspaper packaging on extending the shelf life of lime fruits, addressing a significant gap in postharvest storage research for non-climacteric fruits such as lime.

Effect of Different Growing Media on Establishment of Kagzi Lime (Citrus aurantifolia Swingle) in Prayagraj Agroclimatic Condition

Effect of Different Growing Media on Establishment of Kagzi Lime (Citrus aurantifolia Swingle) in Prayagraj Agroclimatic Condition

Antibacterial Activity for Synthesized Coumarin Derivatives and a Coumarin Component of Lime Peel (Citrus aurantifolia).

In this study, we investigated the antibacterial activity of the coumarin component isolated from lime peel and coumarin derivatives synthesized using various techniques against eight types of food-poisoning bacteria. The minimum inhibitory concentration (MIC) for the 3b [5,7-dihydroxy-4-trifluoromethylcoumarin] derivative was measured as 1.5 mM in Bacillus cereus, Micrococcus luteus, Listeria monocytogenes, and Staphylococcus aureus subsp. aureus; that for the 3c [7-hydroxy-4-trifluoromethylcoumarin] derivative was 1.7 mM in Enterococcus facium; and that for the 3n [dicoumarol] derivative was 1.2 mM in L. monocytogenes. These results confirmed that coumarin derivatives with CF3 and OH substituents had enhanced antibacterial activity.

Properties of Key Lime Essential Oil Blend into Polylactide Acid/Polyethylene Glycol Film Composite

Properties of Key Lime Essential Oil Blend into Polylactide Acid/Polyethylene Glycol Film Composite

Effect of Different Growing Media and Varying Levels of Gibberellic Acid on Seed Germination and Vigour of Acid Lime (citrus aurantifolia swingle.) cv.Kagzi Lime

Effect of Different Growing Media and Varying Levels of Gibberellic Acid on Seed Germination and Vigour of Acid Lime (citrus aurantifolia swingle.) cv.Kagzi Lime

In vitro Propagation of &lt;i&gt;Citrus aurantifolia&lt;/i&gt; and its Conservation in Pakistan

Citrus varieties are propagated by both asexual and sexual methods. Generally, rootstocks are propagated sexually through seeds, while most of the commercial varieties are propagated by various asexual methods such as grafting and budding. The technology called plant tissue culture is used for commercial production of plants that are microbe and virus free and to save germplasm of infrequent or threatened species of plants. Fast reproduction techniques have the potential to produce plants free of viruses. Likewise, traditional propagation methods are old, time consuming and are difficult. Tissue culture techniques are assumed for reliable commercial production of economically important plants. In present study shoot tips of Citrus aurantifolia were collected from the field of BCI, NARC. Different disinfectant (Ethanol and NaOCl) with the concentration ranging from 10 to 20 percent were used for culture establishment. Maximum survival percentage was observed in 10% NaOCl. After 5 weeks, established cultures were shifted to hormonal growth media (BAP, GA3 and NAA) at the concentration ranging from 0.1 to 0.8 mg/L. Our finding results that maximum phenotypic characters (plant height, number of leaves and number of shoots were observed on BAP at the concentration of 0.1 to 0.3 mg/L, while maximum number of roots were observed on NAA at the concentration of 0.5mg/L. In present study, for in vitro conservation different conservants like sorbitol and mannitol (10, 20 and 30 g/L) were used. Incubation of cultures was maintained at 21°C under the light intensity of 1000 lux. Old cultures of Citrus aurantifolia were shifted in conservant media. Overall, remarkable difference was observed. In present study mannitol at the concentration of (10, 20 and 30 g/L) showed slow growth after 35 days. In future the plants in conservation media will be used for further propagation and for growing them in fields.

Effect of Epidermal Mucous Secretion of Earthworms and other Selected Organic Substances on Rooting of Semi-Hardwood Cuttings of &lt;em&gt;Citrus aurantifolia&lt;/em&gt; and Top Cuttings of &lt;em&gt;Dracaena sanderiana&lt;/em&gt;

A recent trend in plant growth and propagation is to use natural substances rather than synthetic plant growth regulators. Two experiments were conducted to find out the effect of epidermal mucous secretion (EMS) of earthworms and other organic substances including Aloe vera gel, coconut water and ripe banana on rooting of semi-hardwood cuttings of Citrus aurantifolia and top cuttings of Dracaena sanderiana compared to commercially available plant growth regulators (PGRs). Eleven treatments were prepared using sole application of EMS solution, Aloe vera gel, coconut water and ripe banana, and mixtures of EMS and other organic substances in two different ratios by allocating equal and more inputs from EMS. The present experiments were set up adopting a completely randomized design. The number of roots per cutting, total root length per cutting (cm), length of the longest root per cutting (cm) and root vigor score were recorded for both plant species where destructive sampling was done at two months after planting for C. aurantifolia and one and two months after planting for D. sanderiana. The number of roots per cutting, length of the longest root per cutting and the root vigor score were significantly greater in the control treatment that used commercially available PGR and the cuttings treated with EMS: coconut water (1:1) for C. aurantifolia suggesting the possibility to us EMS and coconut water mixture to substitute the PGR for root induction in C. aurantifolia. The number of roots, total root length and the length of the longest root per cutting were significantly high in the treatments administrated with Aloe vera gel, ripe banana, and a mixture of EMS: ripe bananas (1:1) together with the PGR at two months after planting for D. sanderiana. Hence, the ingredients listed above could be utilized as alternative organic substances to produce adventurous roots in D. sanderiana top cuttings as same as the PGR. The results of the present study could be extremely valuable for developing a unique organic rooting replacement for the synthetic root inducing materials for C. aurantifolia and D. sanderiana. Nonetheless, additional research is required to validate the results of this study.

Study on biological activity of Lavandula angustifolia and Citrus aurantiifolia essential oils

Abstract The essential oils of Lavandula angustifolia (lavender) and Citrus aurantiifolia (lime), have been used in several applications such as food industry, pharmacy, and cosmetics industries. In this report, we examined the biological activities of lavender and lime essential oils through several studies using β-carotene bleaching assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, antidiabetic assay, antibacterial assay, and also measure its total phenolic content (TPC). The functional group of lavender and lime was studied using Fourier transform infrared spectroscopy (FTIR), while the volatile compound was evaluated using Gas chromatography–mass spectrometry (GC-MS) analysis. The result showed that in DPPH assay, both of lavender and lime essentials oil have low activity with IC 50 of 1966.02 ppm and 4303.42 ppm, respectively, while in beta-carotene bleaching assay, lime has higher antioxidant activity of 39.03% compared to lavender as of 17.55%. Antibacterial activity against S. aureus showed that both lavender and lime have good activity as of 18.13 mm and 18.06 mm, respectively. The antidiabetic activity using α-glucosidase inhibitory activity presented that lavender and lime had high activity of 89.58 % and 88.30 %, respectively. Furthermore, the total phenolic content of lavender was slightly higher as of 480.00 mg GAE/g compared to lime as of 393.33 mg GAE/g. The GC-MS analysis of essential oils showed that lavender contains linalyl acetate (38.78%), linalool (36.12%), and 1,8-cineole (8.38%), while lime contains d-limonene (73.72%), beta-pinene (9.83%) and gamma-terpinene (8.24%), respectively. The present study showed the potential use of lavender and lime essential oils for antidiabetic, antimicrobial, and antioxidant agents.

Enhancing antioxidant properties of lime juice powder through polyelectrolyte microparticles of chitosan-alginate: Formulation, characterization and stability study.

Lime (Citrus aurantifolia) juice was reported to contain ascorbic acid (AA) and flavonoids, which has bioactivity as antioxidants. To develop an antioxidant product, improving its stability is necessary due to the perishable characteristics of compounds in lime. Therefore, the formulation of polyelectrolyte microparticles using chitosan and alginate was conducted to overcome the weaknesses. This study aims to evaluate the effect of various chitosan, alginate, and lime juice powder (LJP) concentrations on the physical characteristics and antioxidant activity of LJP encapsulated in chitosan-alginate microparticles (CALM). Microparticles with various concentrations of chitosan and alginate were prepared by ionic gelation method using CaCl2 as a crosslinker. The microparticles were evaluated for its physical properties and its antioxidant activity using 2-2-diphenyl-1-picrylhydrazyl reagent. A one-way ANOVA test and Tukey's honest significant difference post hoc were used to determine the effect of LJP amount on the antioxidant activity. The highest AA content in CALM was 0.14 mg/100 mg, with a % encapsulation efficiency of 18.38% ± 0.02%. Antioxidant activity tests revealed that LJP possessed the strong antioxidant activity with an IC50 value of 32.59 μg/mL, whereas IC50 values of the microparticles ranged from 24.79 ± 0.03 μg/mL to 39.96 ± 0.07 μg/mL. During storage, the IC50 of LJP decreased from 32.59 ± 0.13 μg/mL to 65.53 ± 0.03 μg/mL, whereas the IC50 of microparticles remained stable. This study concluded that the chitosan-alginate polyelectrolyte microparticle formulation can improve and protect LJP's antioxidant activity.

Evaluating the interaction response of broiler chickens to in ovo feeding of Citrus aurantiifolia seed extract and its powder supplementation

This study aimed to evaluate the effect of in ovo injection and post-hatch supplementation of Citrus aurantiifolia seed on hatchability, growth performance, antioxidant capacity, immune organs weight, and intestinal morphology of Cobb 500 broilers chickens. On day 18 of incubation, 450 fertile eggs were randomly allocated into three sets: non-injected group, 0.5 µg/ml Citrus aurantiifolia seed extract (CASE) group and 0.75 µg/ml CASE group. After hatch, each set was allocated into two groups (5 replicates of 10 birds/replicate each) and was fed on a standard diet with or without Citrus aurantiifolia seed powder (CASP) supplementation (5 g/kg). The groups were divided as follows: IoCA0-C, IoCA0-CA, IoCA5-C, IoCA5-CA, IoCA75-C and IoCA75-CA where IoCA0-C was: non-injected group fed standard diet (negative control group), IoCA0-CA: non-injected and fed CASP supplemented diet (positive control group), IoCA5-C: 0.5 µg/ml of CASE-injected and fed standard diet, IoCA75-C: 0.75 µg/ml of CASE-injected and fed standard diet, IoCA5-CA: 0.5 µg/ml of CASE-injected and fed CASP supplemented diet and IoCA75-CA: 0.75 µg/ml of CASE-injected and fed CASP supplemented diet. The results revealed that the interaction between CASE in ovo injection at 0.5 µg/ml and dietary CASP supplementation at 5 g/kg in the diet increased body weight and weight gain (p < 0.001). The interaction between CASE in ovo injection and dietary CASP supplementation decreased the serum malondialdehyde concentration, the relative weight of the thymus and improved duodenum and ileum villus height (p < 0.05). In conclusion, in ovo injection and dietary supplementation of Citrus aurantiifolia seed powder can improve growth performance and the intestinal morphology of broiler chickens.

RESPONSE OF Citrus aurantifolia TO THE INHIBITION ZONE OF THE Aeromonas salmonicida BACTERIA IN TILAPIA (Oreochromis niloticus)

The impact of using synthetic antibacterials creates a number of new cultivation problems, including: Bacterial resistance, retention of toxic substances and residues in the consumer's body. You need antimicrobial ingredients that can kill bacteria, one of which is the use of lime juice. The aim is to find out how strongly Citrus aurantifolia inhibits the bacteria Aeromonas salmonicida from Oreochromis niloticus. Using a completely randomized design (CRD) with five treatments. The results showed that the diameter of the inhibition zone for each Treatment A was 10.8 mm, Treatment B 12.8 mm, Treatment C 14.7 mm, Treatment D 16.7 mm, and Treatment E 19.1 mm. The relationship between administration of lime juice and the average diameter of the inhibition zone produced in inhibiting the growth of Aeromonas salmonicida bacteria shows a response that increases with increasing dose. Based on the Anova test using F (0.000) &gt; F table at the 5% level of significance, lime juice (Citrus aurantifolia) has an effect on inhibiting the growth of Aeromonas salmonicida in each treatment.

Formulation of Handwash Essential Oil and Hydrosol of Lime Peel (Citrus aurantifolia) as Antibacterials

Formulation of Handwash Essential Oil and Hydrosol of Lime Peel (Citrus aurantifolia) as Antibacterials

Efektivitas Variasi Konsentrasi Air Perasan Jeruk Nipis sebagai Pengganti Komposisi Larutan Turk untuk Hitung Jumlah Leukosit

Standard turk solution is a solution used for manual leukocyte count examination including glacial acetic acid, gentian violet and distilled water. Lime fruit (Citrus aurantifolia swingle) is a type of orange that has a pH of 2.0. Both of these materials belong to weak acids that can lyse blood cells other than leukocytes which can be used to check leukocyte counts. The purpose of this study was to determine the effectiveness of lime juice as a substitute for turk solution with several concentrations. The research design used is descriptive. This study uses the SPSS method. The results of this study were that the sig value of the turk solution with several variations was 2% = 0.935, 3% = 0.326, 4% = 0.162 and 5% = 0.108 where all results were &gt; 0.05 which can be concluded that there was no much difference between the control and all variation of orange juice but the highest significant variation is 2%.

Scenting serenity: influence of essential-oil vaporization on dental anxiety - a cluster-randomized, controlled, single-blinded study (AROMA_dent)

Dental fear and anxiety (DFA) is known as an immense challenge in oral healthcare, which can result in compromised oral health, pain, and uncomfortable treatment. The objective of this study was to analyze the effect of essential-oil vaporization on acute anxiety of patients in dental practices. Four dental practices used five weekly cycles of vaporization with each scent: Orange (Citrus sinensis), Swiss Pine (Pinus cembra), Good Mood (blended essential oils: Citrus sinensis, Citrus aurantifolia, Citrus limon, Osmanthus fragrance (5%)), Forest Walk (blended essential oils: Abies grandis, Pinus cembra, Myrtus communis c. t. 1,8-cineol, Abies alba, Citrus paradisi, Abies sibirica, Pseudotsuga menziesii, Vetiveria zizanoides), and water. Acute anxiety was the primary outcome (state-trait-anxiety inventory (STAI-S)). Secondary outcomes were trait anxiety (STAI-T), dental anxiety (Kleinknecht dental fear survey), and pain perception in treatment (numeric rating scale). Across all patients (n = 486), STAI-S was slightly higher in the control group (40.7 ± 11.6) than in the intervention groups (38.4 ± 10.5). Post-hoc analyses revealed that the effect is only robust for the subgroup of female patients (n = 296, p = 0.044). We also conducted a post-hoc additional analysis on a subpopulation with an increased level of STAI-T ≥ 42 (n = 131 patients). For this group the difference in acute anxiety between the control group (51.1 ± 11.9, n = 30) vs. the intervention groups (46.8 ± 9.6, n = 118) was significant (T = 4.39, p = 0.0379). The results of the study indicate a promising potential of essential-oil vaporization to alleviate dental anxiety, particularly in the subgroups of patients with a high level of trait anxiety, and particularly in female patients. The calming effects of the essential-oil vaporization were also highlighted by the anecdotical statements of the dental-practice staff. The anxiety-reducing role of essential-oil vaporization alone and as one part of combined techniques to counter DFA should be further explored using multi-perspective methodological approaches in research.