Article1 September 1944SUBCLINICAL VITAMIN DEFICIENCY V. THE ASSAY OF SUBCLINICAL THIAMIN DEFICIENCYMILDRED CARLEEN HULSE, M.A., NORMAN WEISSMAN, PH.D., ELMER STOTZ, PH.D., MARSHALL CLINTON, M.D., JOSEPH W. FERREBEE, M.D.MILDRED CARLEEN HULSE, M.A.Search for more papers by this author, NORMAN WEISSMAN, PH.D.Search for more papers by this author, ELMER STOTZ, PH.D.Search for more papers by this author, MARSHALL CLINTON, M.D.Search for more papers by this author, JOSEPH W. FERREBEE, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-21-3-440 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptINTRODUCTIONThe assay of subclinical thiamin deficiency is primarily a problem in defining changes in thiamin nutrition before signs or symptoms of deficiency make their appearance. Accomplishment of this end presupposes a knowledge of normal thiamin nutrition; that is, normal extracellular and intracellular thiamin concentrations, and the size and sequence of variation in these values which may precede the occurrence of clinical deficiency.1The purpose of the present study is to determine whether measurable changes in either extracellular or intracellular thiamin concentrations occur in subclinical thiamin deficiency. For comparison, observations are also reported on thiamin excretion and on pyruvate metabolism....Bibliography1. HULSEWEISSMANOWENFERREBEE MCNPSJW: Subclinical vitamin deficiency, Science, 1943, xcvii, 47. Google Scholar2. ATKINSCHULTZFREY LASCN: Ultramicrodetermination of thiamin by the fermentation method, Jr. Biol. Chem., 1939, cxxix, 471. CrossrefGoogle Scholar3. SCHULTZATKINFREYWILLIAMS ASLCNRR: Application of the sulfite cleavage of thiamin to the yeast fermentation method, Jr. Am. Chem. Soc., 1941, lxiii, 632. CrossrefGoogle Scholar4. HULSEWEISSMANROWLANDGROSSFERREBEE MCNVRJW: Subclinical vitamin deficiency. VI. Thiamine in skeletal muscle of infants and children, Am. Jr. Dis. Child., 1944, lxvii, 30. CrossrefGoogle Scholar5. HULSEWEISSMANFERREBEE MCNJW: Subclinical vitamin deficiency. IV. Plasma thiamin, Jr. Clin. Invest., 1944, xxiii, 297. Google Scholar6. KUTTNERLICHTENSTEIN TL: Micro colorimetric studies, II, Jr. Biol. Chem., 1930, lxxxvi, 671. CrossrefGoogle Scholar7. FERREBEECARDEN JWGA: A procedure for the routine determination of vitamin B1 in urine, Jr. Lab. and Clin. Med., 1940, xxv, 1320. Google Scholar8. ROBINSONMELNICKFIELD WDDH: Urinary excretion of thiamin in clinical cases and the value of such analyses in the diagnosis of thiamin deficiency, Jr. Clin. Invest., 1940, xix, 399. CrossrefGoogle Scholar9. BUEDINGWORTIS EH: The stabilization and determination of pyruvic acid in the blood, Jr. Biol. Chem., 1940, cxxxiii, 585. CrossrefGoogle Scholar10. LUPLATT GDBS: The metabolism of pyruvic acid in normal and vitamin B1-deficient states. V. The effect of exercise on blood pyruvate in vitamin B1 deficiency in man, Biochem. Jr., 1939, xxxiii, 1538. CrossrefGoogle Scholar11. BUEDINGSTEINWORTIS EMHH: Blood pyruvate curves following glucose ingestion in normal and thiamin-deficient subjects, Jr. Biol. Chem., 1941, cxl, 697. CrossrefGoogle Scholar12. STOTZBESSEY EOA: The blood lactate-pyruvate relation and its use in experimental thiamin deficiency in pigeons, Jr. Biol. Chem., 1942, cxliii, 625. CrossrefGoogle Scholar13. WILLIAMSMASONWILDERSMITH RDHLRMBF: Observations on induced thiamine (vitamin B1) deficiency in man, Arch. Int. Med., 1940, lxvi, 785. CrossrefGoogle Scholar14. FERREBEEWEISSMANPARKEROWEN JWNDPS: Tissue thiamin concentrations and urinary thiamin excretion, Jr. Clin. Invest., 1942, xxi, 401. CrossrefGoogle Scholar15. WILLIAMSMASONWILDER RDHLRM: The minimum daily requirements of thiamine of man, Jr. Nutr., 1943, xxv, 71. CrossrefGoogle Scholar16. JOHNSONDARLINGFORBESBROUHAEGAÑAGRAYBIEL RERCWHLEA: The effects of a diet deficient in part of the vitamin B complex upon men doing manual labor, Jr. Nutr., 1942, xxiv, 585. CrossrefGoogle Scholar17. FERREBEEWEISSMANPARKEROWEN JWNDPS: The thiamin content of human tissue, Res. Pub. Assoc. Nerv. and Ment. Dis., 1943, xxii, 42. Google Scholar18. PUCHERSHERMANVICKERY GWCCHB: A method to determine small amounts of citric acid in biological material, Jr. Biol. Chem., 1936, cxiii, 235. CrossrefGoogle Scholar19. SOBERLIPTONELVEHJEM HAMACA: The relation of thiamine to citric acid metabolism, Jr. Biol. Chem., 1940, cxxxiv, 605. CrossrefGoogle Scholar20. KREBS HA: The biochemical lesion in vitamin-B1 deficiency, Chem. and Ind., 1938, lvii, 213. Google Scholar21. EVANS EA: The biochemical action of the vitamins, 1942, The University of Chicago Press, Chicago. Google Scholar22. NAJJARHOLT VALE: Studies in thiamin excretion, Bull. Johns Hopkins Hosp., 1940, lxvii, 107. Google Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Boston, Massachusetts*Received for publication September 20, 1943.From the Laboratory of Dental Medicine, Harvard School of Dental Medicine; the Medical Clinic, Peter Bent Brigham Hospital, and the Biochemical Laboratory of the McLean Hospital, Boston, Massachusetts.Aided by grants from the Williams and Waterman Fund, Research Corporation, New York City, and the Nutrition Foundation, New York City.This report is a preliminary study whose completion has been delayed by other work related to the war effort. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 September 1944Volume 21, Issue 3Page: 440-446KeywordsAttentionExcretionHospital medicineNutritionPyruvateResearch fundingResearch laboratoriesSigns and symptomsThiamine Issue Published: 1 September 1944 PDF downloadLoading ...