Citrate Synthesis Research Articles

Iron and Nickel Substituted Perovskite Cobaltites for Sustainable Oxygen Evolving Anodes in Alkaline Environment.

Perovskite oxides have great flexibility in their elemental composition, which is accompanied by large adjustability in their electronic properties. Herein, we synthesized twelve perovskite oxide-based catalysts for the oxygen evolution reaction (OER) in alkaline media. The catalysts are based on the parent oxide perovskite Ba0.5Gd0.8La0.7Co2O6-δ (BGLC587) and are synthesized through the sol-gel citrate synthesis route. To reduce the demand on cobalt (Co), but also increase the intrinsic catalytic activity of BGLC587 for the OER, we substitute Co on the B-site with certain amounts of Fe and Ni, synthesizing catalysts of the general formula Ba0.5Gd0.8La0.7Co2-x-yFexNiyO6-δ. A plethora of physicochemical and electrochemical methods suggest that an Fe content between 30 % and 70 % increases the intrinsic catalytic activity of BGLC587, while Tafel slopes in combination with in-situ Raman spectroscopy suggest the rate determining step is likely a proton-exchange reaction, progressing possibly through the lattice oxygen mechanism (LOM). We apply one of the optimized, Co-substituted perovskites in a monolithic, photovoltaic (PV)-driven electrolysis cell and we achieve an initial solar-to-hydrogen (STH) conversion efficiency of 10.5 % under one sun solar simulated illumination.

Synthesis of La2Ti2O7 nanoscale powder and ceramics based on it by sol-gel and spark plasma sintering

The application of ceramics as matrices for the immobilization of radionuclides for the purpose of their safe long-term disposal or beneficial use is being studied with an emphasis on phase stability, structural integrity, hydrolytic stability, etc. In this work, a combined approach was investigated, based on the sol-gel citrate synthesis of nanosized La2Ti2O7 powder and its subsequent spark plasma sintering to produce dense ceramics. The phase composition and structure of the nanosized La2Ti2O7 powder and ceramic samples based on it, obtained in the temperature range of 900–1300 °C, were studied by XRD and SEM. It has been shown that the powder synthesis conditions ensure the formation of nanosized crystalline La2Ti2O7 grains, whose consolidation under spark plasma heating conditions proceeds with a change in the phase composition from single-phase La2Ti2O7 of monoclinic structure to orthorhombic with an admixture of LaTiO3 at temperatures above 1200 °C. It was revealed that the change in the ceramic structure is accompanied by the formation of non-porous and defect-free monolithic samples. It was determined that such a change leads to an increase in relative density (81.3–95.7%) and compressive strength (78–566 MPa) of the ceramic samples. However, the hydrolytic stability of the ceramics decreases, as indicated by an increase in the leaching rate of La3+ from 10–7 to 10–5 g/cm2·day. The obtained results are useful for the systematic study of materials suitable for immobilization technologies of radioactive waste in ceramics.

Iron citrate Synthesit: impact on blood parameters in non-iron deficient models

Iron's significance extends beyond treating anemia, impacting various disorders. However, studies on iron-fortified diets in healthy subjects are limited. To evaluate the effects of Synthesit iron citrate on biochemical, hematological parameters, and body weight in aged rhesus macaques, and its safety in SHK-line mice. An experimental study involved SHK mice and rhesus macaques receiving Synthesit iron citrate, with blood parameters, histology, hematopoietic stem cells, and biochemistry monitored over 30 days (14 mice) and six weeks (8 macaques). The study shows increased hematopoietic cell types and mitosis index, with significant increases in Myelokaryocyte count (32.4%), megakaryocyte count (3.7-fold), myeloblast count (1.7-fold), mononuclear cell count (2.5-fold), erythroblast count (4-fold), and reticulocytes (13.3%). In macaques, supplementation initially reduced erythrocyte count, followed by recovery, while thrombocyte count increased initially but decreased post-supplementation. Leukocyte analysis showed varied responses, with a decrease in monocyte and lymphocyte but an increase in neutrophil percentage. Biochemical analyses revealed improvements in glucose (baseline: 4.36 ± 0.32 mmol/l vs day 43: 3.30 ± 1.06 mmol/l), cholesterol (baseline: 4.563 ± 0.799 mmol/l vs day 29: 2.84 ± 0.88 mmol/l), and triglyceride levels (baseline: 1.49 ± 0.63 mmol/l vs day 29: 0.70 ± 0.34 mmol/l). The novel study reveals the effects of iron citrate synthesis in non-iron deficient models into the blood parameters which has metabolic potential. In conclusion, Synthesit® iron citrate supplementation exhibits promising effects on hematopoiesis and metabolic parameters in murine and primate models.

Structural and biophysical insights into the interactions of anisotropic gold nanoparticles with human telomeric G-quadruplex DNA: Spectroscopic and calorimetric approach

We are reporting curcumin-induced synthesis of anisotropic citrate capped Gold nanoparticles (ctGNPs). The techniques such as UV–visible spectroscopy, Raman spectroscopy, FT-IR, X-ray diffraction (XRD), and Transmission Electron Microscopy (TEM) were used to characterize the nanoparticles. The synthetic route shows the formation of an anisotropic gold nanostructure consisting of spherical, triangles, hexagonals, and a low-yield rod with an aspect ratio ranging from 4.2 to 8.5. Curcumin-derived ctGNPs shows stability at different physiological conditions and better biocompatibility. Synthesized nanoparticles are found non-toxic towards eukaryotic cells but more effective against the cancer cell lines HeLa and MCF-7. The interaction of these synthesized nanoparticles with human telomeric G-quadruplex (GQ) DNA was studied using different physiochemical methods. The spectroscopic studies show that synthesized nanoparticles have stronger binding affinity towards telomeric GQ as compared to ds DNA through Van der Waals and H-bonding interactions. Thermodynamic interpretation reveals that the formation of the complex between the telomeric GQ and ctGNPs are enthalpy driven and entropy unfavourable process, resulting in motion freezing and, eventually, AuNP aggregation. Thus, our study shows a new approach to understand the interaction of telomeric G-quadruplexes with gold nanoparticles generated via the green route.

Comparative transcriptome analyses reveal regulatory network and hub genes of aluminum response in roots of elephant grass (Cenchrus purpureus)

Aluminum (Al) toxicity severely restricts the growth and productivity of elephant grass in acidic soils around the world. However, the molecular mechanisms of Al response have not been investigated in elephant grass. In this study, we conducted phenotype, physiology, and transcriptome analysis of elephant grass roots in response to Al stress. Phenotypic analysis revealed that a low concentration of Al stress improved root growth while higher Al concentrations inhibit root growth. Al stress significantly increased the citrate (CA) content in roots, while the expression levels of genes related to citrate synthesis were substantially changed. The multidrug and toxic compound extrusion (MATE) family were identified as hub genes in the co-expression network of Al response in elephant grass roots. Phylogenetic analysis showed that hub genes CpMATE93 and CpMATE158 belonged to the same clade as other MATE genes reported to be involved in citrate transport. Additionally, overexpression of CpMATE93 conferred Al resistance in yeast cells. These results provide a theoretical basis for further studies of molecular mechanisms in the elephant grass response to Al stress and could help breeders develop elite cultivars with Al tolerance.

Water-Doped Brønsted Acidic Protic Ionic Liquids for Enhanced Tributyl Citrate Synthesis in a Two-Phase Esterification System.

Tributyl citrate (TBC) plays a crucial role as a plasticizer, enhancing the flexibility of polymers such as polyvinyl chloride. Its biodegradability and non-toxic nature contribute to eco-friendly appeal, making it a preferred additive in diverse industries, including food packaging, medical devices, toys, and consumer goods. Herein, a method for the synthesis of TBC using inexpensive Brønsted acidic protic ionic liquids (ILs) in a two-phase reaction system is presented. The esterification process is carried out with high yield (>99 %), selectivity (up to 98 %) and short reaction time of 2 h. The catalyst in the form of IL shows excellent performance and stability, desirable for industrial applications.

Integration of Science and Education: Investigation of Tributyl Citrate Synthesis under the Promotion of Hydrate Molten Salts for Research and Innovation Training

Integration of Science and Education: Investigation of Tributyl Citrate Synthesis under the Promotion of Hydrate Molten Salts for Research and Innovation Training

Nanogold-Bound Copper Complexes and Their Various Applications: A Review Article

The term "nano gold," also known as "gold nanoparticles," is commonly used. These particles are extremely small, with a diameter of less than 100 nm, which is only a fraction of the width of a human hair. Due to their tiny size, nano gold particles are often found in a colloidal solution, where they are suspended in a liquid stabilizer. This colloidal gold is essentially another name for nano gold. The main method for producing gold nanoparticles in a colloidal solution is the citrate synthesis technique, which involves combining different solutions to precipitate the gold nanoparticles[1-5]. In biological systems, copper complexes play a significant role at the active sites of many metalloproteins. These complexes have potential applications in various catalytic processes that occur in living organisms, such as electron transfer reactions and the activation of specific antitumor substances. These processes are relevant in the fields of medicinal chemistry and bioinorganic chemistry. The interaction of copper chelates with biological systems and their noteworthy activities against neoplastic, bacterial, fungal, and cancerous cells are also important. Many copper (II) N, S, O / N, N-donor chelators function as effective anticancer agents due to their ability to bind with DNA base pairs[6-10]. Using hydrophilic gold nanoparticles (AuNPs) as carriers for copper complexes is a novel and purposeful strategy that Could raise these compounds' stability and solubility in H2O aqueous., thus enhancing their bioavailability. The regulated release of Cu-complexes made possible by this method also creates the possibility for fruitful in vivo and in vitro tests. The definition, significance, and numerous applications of copper complexes in connection to nanogold are presented in this review study[11-15].

CcNFYB3-CcMATE35 and LncRNA CcLTCS-CcCS modules jointly regulate the efflux and synthesis of citrate to enhance aluminium tolerance in pigeon pea.

Aluminium (Al) toxicity decreases crop production in acid soils in general, but many crops have evolved complex mechanisms to resist it. However, our current understanding of how plants cope with Al stress and perform Al resistance is still at the initial stage. In this study, the citrate transporter CcMATE35 was identified to be involved in Al stress response. The release of citrate was increased substantially in CcMATE35 over-expression (OE) lines under Al stress, indicating enhanced Al resistance. It was demonstrated that transcription factor CcNFYB3 regulated the expression of CcMATE35, promoting the release of citrate from roots to increase Al resistance in pigeon pea. We also found that a Long noncoding RNA Targeting Citrate Synthase (CcLTCS) is involved in Al resistance in pigeon pea. Compared with controls, overexpression of CcLTCS elevated the expression level of the Citrate Synthase gene (CcCS), leading to increases in root citrate level and citrate release, which forms another module to regulate Al resistance in pigeon pea. Simultaneous overexpression of CcNFYB3 and CcLTCS further increased Al resistance. Taken together, these findings suggest that the two modules, CcNFYB3-CcMATE35 and CcLTCS-CcCS, jointly regulate the efflux and synthesis of citrate and may play an important role in enhancing the resistance of pigeon pea under Al stress.

Nitrate improves aluminium resistance through SLAH-mediated citrate exudation from roots.

Aluminium (Al) toxicity is one of the major constraint for crop production in acidic soil, and the inappropriate utilization of nitrogen fertilizer can accelerate soil acidification. Despite previous studies investigating the regulation of nitrogen forms in Al toxicity of plants, the underlying mechanism, particularly at the molecular level, remains unclear. This study aims to uncover the potentially regulatory mechanism of nitrate (NO3 - ) in the Al resistance of maize and Arabidopsis. NO3 - conservatively improves Al resistance in maize and Arabidopsis, with nitrate-elevated citrate synthesis and exudation potentially playing critical roles in excluding Al from the root symplast. ZmSLAH2 in maize and AtSLAH1 in Arabidopsis are essential for the regulation of citrate exudation and NO3 - -promoted Al resistance, with ZmMYB81 directly targeting the ZmSLAH2 promoter to activate its activity. Additionally, NO3 - transport is necessary for NO3 - -promoted Al resistance, with ZmNRT1.1A and AtNRT1.1 potentially playing vital roles. The suppression of NO3 - transport in roots by ammonium (NH4 + ) may inhibit NO3 - -promoted Al resistance. This study provides novel insights into the understanding of the crucial role of NO3 - -mediated signalling in the Al resistance of plants and offers guidance for nitrogen fertilization on acid soils.

Inosine enhances tumor mitochondrial respiration by inducing Rag GTPases and nascent protein synthesis under nutrient starvation

Metabolic heterogeneity of tumor microenvironment (TME) is a hallmark of cancer and a big barrier to cancer treatment. Cancer cells display diverse capacities to utilize alternative carbon sources, including nucleotides, under poor nutrient circumstances. However, whether and how purine, especially inosine, regulates mitochondrial metabolism to buffer nutrient starvation has not been well-defined yet. Here, we identify the induction of 5′-nucleotidase, cytosolic II (NT5C2) gene expression promotes inosine accumulation and maintains cancer cell survival in the nutrient-poor region. Inosine elevation further induces Rag GTPases abundance and mTORC1 signaling pathway by enhancing transcription factor SP1 level in the starved tumor. Besides, inosine supplementary stimulates the synthesis of nascent TCA cycle enzymes, including citrate synthesis (CS) and aconitase 1 (ACO1), to further enhance oxidative phosphorylation of breast cancer cells under glucose starvation, leading to the accumulation of iso-citric acid. Inhibition of the CS activity or knockdown of ACO1 blocks the rescue effect of inosine on cancer survival under starvation. Collectively, our finding highlights the vital signal role of inosine linking mitochondrial respiration and buffering starvation, beyond serving as direct energy carriers or building blocks for genetic code in TME, shedding light on future cancer treatment by targeting inosine metabolism.

Transcriptomic and metabolite analyses provided a new sight of 1-MCP on organic acid metabolism in peach during storage.

In this study, "Xiahui 6" peaches were treated with 10µL/L 1-methylcyclopropene (1-MCP) for 12h and then stored at 20°C for 9 days; the regulation of 1-MCP on organic acids during storage was investigated through transcriptomic and metabolite analyses. Results showed that 1-MCP maintained higher gene expression of malate synthesis (PpPEPC1, PpPEPC2, and PpNAD-cytMDH) at the end of storage but extremely inhibited the gene expression of malate degradation (PpNADP-cytME) during storage, resulting that malate content in treated peaches was twice that of control group at day 7. Besides, the increasement of citrate synthesis and degradation-related genes (PpmitCS, PpcytACO, PpNAD-mitIDH, and PpNADP-cytIDH) at days 3 and 5 was postponed by 1-MCP treatment, accompanied by 0.5 times higher citrate content at day 7. Our results suggested that 1-MCP has inhibitory effects on both the synthesis and degradation of organic acids; however, the inhibitory effect of 1-MCP on organic acid degradation may be greater than that on organic acid synthesis. Practical Application: This study provides a theoretical basis for the application of 1-methylcyclopropene (1-MCP) in fruit preservation.

Transient response to perturbations in flow synthesis of citrate capped gold nanoparticles

This work reports the transient behavior of continuous flow synthesis of gold nanoparticles (Au NPs) when subjected to perturbations in operating conditions using controlled experiments. The intricacies are captured through a detailed mathematical model. Reversed Turkevich protocol was used for synthesis of Au NPs. The synthesis was first studied in batch mode to investigate the reaction kinetics and reproducibility of the process. The optimal set of operating conditions viz., residence time, flow rate, temperature was then used for flow synthesis in a 2 m, 1/16″ Polytetrafluoroethylene (PTFE) reactor with micromixer. Reactor clogging was avoided by using segmented flow. Inline UV measurement was used for real time monitoring of the process. Transient experiments were performed by abruptly changing the operating conditions. A mathematical model was found to be accurate in predicting the transient behavior of the exit precursor concentration and the particle size for unsteady state synthesis. Even a small change in process variables for short duration was found to disturb the quality of Au NPs for a significantly longer duration. Of the three operating parameters, the effect of temperature variation was seen to have a prolonged effect where the system remained in unsteady state for long time.

Persistent fasting lipogenesis links impaired ketogenesis with citrate synthesis in humans with nonalcoholic fatty liver.

BACKGROUNDHepatic de novo lipogenesis (DNL) and β-oxidation are tightly coordinated, and their dysregulation is thought to contribute to the pathogenesis of nonalcoholic fatty liver (NAFL). Fasting normally relaxes DNL-mediated inhibition of hepatic β-oxidation, dramatically increasing ketogenesis and decreasing reliance on the TCA cycle. Thus, we tested whether aberrant oxidative metabolism in fasting NAFL subjects is related to the inability to halt fasting DNL.METHODSForty consecutive nondiabetic individuals with and without a history of NAFL were recruited for this observational study. After phenotyping, subjects fasted for 24 hours, and hepatic metabolism was interrogated using a combination of 2H2O and 13C tracers, magnetic resonance spectroscopy, and high-resolution mass spectrometry.RESULTSWithin a subset of subjects, DNL was detectable after a 24-hour fast and was more prominent in those with NAFL, though it was poorly correlated with steatosis. However, fasting DNL negatively correlated with hepatic β-oxidation and ketogenesis and positively correlated with citrate synthesis. Subjects with NAFL but undetectable fasting DNL (25th percentile) were comparatively normal. However, those with the highest fasting DNL (75th percentile) were intransigent to the effects of fasting on the concentration of insulin, non-esterified fatty acid, and ketones. Additionally, they sustained glycogenolysis and were spared the loss of oxaloacetate to gluconeogenesis in favor of citrate synthesis, which correlated with DNL and diminished ketogenesis.CONCLUSIONMetabolic flux analysis in fasted subjects indicates that shared metabolic mechanisms link the dysregulations of hepatic DNL, ketogenesis, and the TCA cycle in NAFL.TRIAL REGISTRATIONData were obtained during the enrollment/non-intervention phase of Effect of Vitamin E on Non-Alcoholic Fatty Liver Disease, ClinicalTrials.gov NCT02690792.FUNDINGThis work was supported by the University of Texas Southwestern NORC Quantitative Metabolism Core (NIH P30DK127984), the NIH/National Institute of Diabetes and Digestive and Kidney Diseases (R01DK078184, R01DK128168, R01DK087977, R01DK132254, and K01DK133630), the NIH/National Institute on Alcohol Abuse and Alcoholism (K01AA030327), and the Robert A. Welch Foundation (I-1804).

Defective import of mitochondrial metabolic enzyme elicits ectopic metabolic stress

Deficiencies in mitochondrial protein import are associated with a number of diseases. However, although nonimported mitochondrial proteins are at great risk of aggregation, it remains largely unclear how their accumulation causes cell dysfunction. Here, we show that nonimported citrate synthase is targeted for proteasomal degradation by the ubiquitin ligase SCFUcc1. Unexpectedly, our structural and genetic analyses revealed that nonimported citrate synthase appears to form an enzymatically active conformation in the cytosol. Its excess accumulation caused ectopic citrate synthesis, which, in turn, led to an imbalance in carbon flux of sugar, a reduction of the pool of amino acids and nucleotides, and a growth defect. Under these conditions, translation repression is induced and acts as a protective mechanism that mitigates the growth defect. We propose that the consequence of mitochondrial import failure is not limited to proteotoxic insults, but that the accumulation of a nonimported metabolic enzyme elicits ectopic metabolic stress.

An insight into synthesis and antitumor activity of citrate and gallate stabilizing gold nanospheres

Both gallic and citrate are well-established antioxidants that show promise as new selective anti-cancer drugs. Gold nanoparticles (AuNPs) as well can be developed as flexible and nontoxic nano-carriers for anti-cancer drugs. This article evaluating the efficiency and biocompatibility of gallic acid and citrate capping gold nanoparticles to be used as anti-cancer drug. The biosafety and therapeutic efficiency of prepared nano-formulations were tested on Hela and normal BHK cell line. Gold nanospheres coated with citrate and gallate were synthesized via wet chemical reduction method. The prepared nano-formulations, citrate and gallate coated gold nanospheres (Cit-AuNPs and Ga-AuNPs), were characterized with respect to their morphology, FTIR spectra, and physical properties. In addition, to assess their cytotoxicity, cell cycle arrest and flow cytometry to measure biological response were performed. Cit-Au NPs and Ga-Au NPs were shown to significantly reduce the viability of Hela cancer cells. Both G0/G cell cycle arrest and comet assay results showed that genotoxic effect was induced in Hela cells by Cit-Au NPs and Ga-Au NPs. The results of this study showed that Cit-Au NPs and Ga-AuNPs inhibit the growth of metastatic cervical cancer cells, which could have therapeutic implications.

Features of triamyl citrate synthesis

Objectives. To find an effective way for obtaining triamyl citrate, an environmentally friendly, biodegradable citric acid ester used as a plasticizer for PVC-based polymer compositions.Methods. The possibilities of heterogeneous catalysis were analyzed using the case study of three commercial samples of macroporous sulfocationites (Amberlyst™ 15, Amberlyst™ 70, and TULSION® 66). Homogeneous catalysis was studied using the example of orthophosphoric acid (H3PO4), while self-catalysis was investigated during esterification of citric acid with amyl alcohol (ROH). The syntheses were carried out under identical conditions: T = 110 °C, the ratio of CA:ROH = 1:5 (mol) amount of catalyst 1 wt % on the reaction mass in a thermostatically controlled reactor of ideal mixing with continuous distillation of the resulting water.Results. It was found that in all variants (even under self-catalysis conditions), the conversion of citric acid in 180 min reached 94–99%. Triamyl citrate was formed after 9 h with a yield of 90% only when using a homogeneous catalyst (H3PO4) and in the presence of a heterogeneous catalyst sample (Amberlyst ™ 15).Conclusions. The revealed differences in the reactivity of the studied sulfocationites (Amberlyst™ 15, Amberlyst ™ 70, and TULSION® 66) confirm the well-known theoretical positions, according to which the kinetic pseudo-homogeneous model of the esterification process of hydroxy acids in excess of aliphatic alcohols is based on the law of acting masses and depends on the specific surface area of the catalyst, which for Amberlyst ™ 15 is of the greatest importance as compared to Amberlyst ™ 70 and TULSION® 66 (m2/g): 53:36:35, respectively.

An Industrial Approach to Production of Tofacitinib Citrate (TFC) as an Anti-COVID-19 Agent: A Joint Experimental and Theoretical Study

In this report, we have presented our experience about a facile method for synthesis of tofacitinib citrate (TFC). The developed analytical methods for identification and qualifications are also included. As TFC seems to be effective in treatment of the symptoms of COVID-19 (SARS family), manufacturing of this active pharmaceutical ingredient (API) could be helpful. The API of TFC was prepared from the diamine intermediate in an ambient and solvent-free condition. Elimination of the reaction solvent resulted in decreasing the cost and preventing the rejection of the organic volatile impurity (OVI) test. The final citrate addition step was carried out using water as a solvent (the citrate content was 37.5% by potentiometry). Moreover, the results of the Karl-Fischer (KF) titration analysis was about 0.24%, which showed that the use of water does not increase the water content of the crystal structure.

Responses to aluminum and cadmium of a RNAi sorghum line with decreased levels of phosphoenolpyruvate carboxylase 3 (PPC3)

Phosphoenolpyruvate carboxylase (PEPC) is an enzyme family with central roles in carbon and nitrogen metabolisms. In order to obtain knowledge about the function of specific PEPC isoenzymes, we have characterized RNAi sorghum lines with decreased level of PPC3 (Ppc3 lines). PPC3 is the main root PEPC, and participates in responses to salinity and ammonium stress. This prompted us to study the responses of Ppc3 lines to Al and Cd stress. Both Al and Cd treatments decreased germination rate, growth rate, and shoot and root biomass production. These detrimental effects were higher in Ppc3 lines than in Wt. Root PPC3 expression and PEPC activity was increased by Al and Cd in Wt, but not in Ppc3 lines. The treatments also increased PPC2 and PPCK gene expression, and PEPCk activity, but these changes did not substitute the lack of PPC3. The production and secretion of exudates by plant roots play an important role in plant tolerance to Al and Cd toxicity. Al (2 days of treatment) caused a 40-fold increase of the citrate content of root exudates, without differences between Wt and Ppc3 lines. On the contrary, Cd (7 days of treatment) caused a 400-fold increase of citrate in Wt exudates, but only a 30-fold increase in Ppc3 lines. These results indicate that although PPC3 is not necessary for early production of organic acids in root exudates, it is crucial for maintaining high level of synthesis and accumulation of citrate, and that PPC3 is the main PEPC isoenzyme responsible for this response to heavy metal stress.

Preparation of porous macromolecule oxazine poly‐ionic liquid solid acid catalyst and its application in an esterification reaction

AbstractBackgroundChinese research on citrate is typically into plasticizer alternatives to the carcinogenic phthalate (PAEs) plasticizers. PAEs plasticizers can induce obesity and metabolic disorders, whereas citric acid vinegar plasticizers have the advantages of low biological toxicity and environmental friendliness.ResultsNovel porous macromolecule oxazine poly‐ionic liquid solid acid catalysts were prepared and characterized by scanning electron microscopy (SEM), Brunner−Emmet−Teller(BET) measurements, Fourier transform infrared spectroscopy (FTIR), energy dispersive spectrometer (EDS) and thermogravimetric analysis (TGA). Characterization results showed that the synthesized catalyst was a porous polymer with good heat stability. The catalytic performance of the catalyst was evaluated by synthesis of tributyl citrate from citric acid and n‐butanol.ConclusionThe optimal reaction conditions were determined as 9% (wt%) citrate mass. The experimental results showed that the optimal reaction conditions were determined as amount of catalyst 9% (wt% citrate mass), 150 °C reaction temperature, 3.5 h reaction time and 1:3 ratio of citric acid and n‐butanol. Under optimal conditions, the conversion rate of citrate acid was ≤91.74%. The catalytic activity did not reduce obviously after being reused for ten times. Then, the reaction conditions were fited by the surface optimization method. The fitted optimum conditions were as follows: 8.881% amount of catalyst (mass of citric acid), 148.259 °C reaction temperature, 3.331 h reaction time, 1:2.958 citric acid:n‐butanol ratio; this gave a conversion rate of citric acid of ≤92.998%. The simulation results are in good agreement with the experimental results. © 2022 Society of Chemical Industry (SCI).