The existences of paracetamol residue in the aqueous environment are strong belief consequences that a negative effect is a real against for all creation surrounding. Wastewater effluents into the Tigris River at Mosul city, Iraq, are often real problem, in particular, which have contained of many pharmaceutical traces. However, the aim of this study was to quantify and qualify the paracetamol based on serological test. Twenty four locations were chosen precisely from wastewater effluent zone of town which goes directly into the River. All samples were estimated according to Enzyme linked Immunosorbent Assay technique. The study planned that have collected samples for the same area for three different times, to focus on the probability investigation different paracetamol levels concentrations. The Results referred that there were simple proportional variations in the concentration of paracetamol in spite of the samples have collected three different times at the same area. It was observed that the concentration of paracetamol at the right bank of Mosul city were high levels ranging from 6 to 9.9 ng /ml for all areas. In contrast, there is a significant fluctuations results starting between 3 to 9.9 ng/ ml of the paracetamol levels for samples at left bank areas. Overall, it is possible to indicate that for all wastwater effluent samples for this study have contaminated with paracetamol traces, which unfortuntly effect directly into the public health envirment of city and human being as well.