Cis-linalool Oxide Research Articles

Essential Oil Composition of Thyme (Thymus zygioides Griseb. var. lycaonicus (Celak.) Ronniger) Wild Growing in Turkey

The chemical composition of essential oil, which is isolated by hydrodistillation of aerial parts of Thymus zygioides growing wild in Turkey, have been determined by GC and GC/MS. Essential oil yield on a dry weight basis of aerial parts from T. zygioides was 0.45%. A total of twenty two constituents were identified by GC-MS analyses, the main components representing 92.3% the oil was identified as limonene (24.11%), cis-linalool oxide (22.91%), eucalyptol (8.65%). 3-octonol (7.04%), nerol (4.47%) geranyl acetate (3.19%), carvacrol (2.74%), linalyl propanoate (2.95%) and sabinene (2.23%). The essential oil of T. zygioides was characterized by its high content of limonene (24.11%).

Semiochemical-mediated aggregation of the ambrosia beetle Trypodendron betulae (Coleoptera: Curculionidae: Scolytinae)

AbstractPorapak Q-captured volatiles from both sexes of Trypodendron betulae Swaine (Coleoptera: Curculionidae: Scolytinae) excised from newly attacked logs of paper birch, Betula papyrifera Marshall (Betulaceae), as well as volatiles from unattacked birch logs, were analysed by coupled gas chromatographic electroantennographic detection analysis. Active compounds were identified by gas chromatographic mass spectroscopy. The enantiomeric ratio of 6-ethenyl-2,2,6-trimethyloxan-3-ol (linalool oxide pyranoid) was determined using a Cyclodex B column. Field-trapping experiments disclosed that the female-produced aggregation pheromone of T. betulae is a blend of the (3S,6R)-trans- and (3R,6R)-cis-linalool oxide pyranoid. Trap catches were synergistically increased when the pheromone was combined with both the host volatile ethanol and with conophthorin, which was found in female beetles as well as host volatiles. Use of linalool oxide pyranoid reproductively isolates T. betulae from sympatric Trypodendron Stephens species for which only (+)-lineatin has been identified as an aggregation pheromone.

Chemical Examination of Essential Oil from Stem, Roots and Fruit Peels of Nigerian Citrus jambhiri

The chemical profiles of the stem, root and fruit peel essential oils of Citrus jambhiri were investigated. Pulverized plant samples (500 g) each was hydrodistilled using all-glass Clevenger-type apparatus to obtain the essential oils. Two-dimensional GC-TOFMS was used for compositional profiling of the extracted oils. Chemical profiling of the stem oil of C. jambhiri revealed the presence of 64 components (83.11 %), the root oil of C. jambhiri showed 55 components (74.21 %) and the fruit peel oil of C. jambhiri revealed 25 components (83.56 %). The major components of the stem oil of C. jambhiri is 3,4-dimethyl-1,5-cyclooctadiene(13.43 %); geijerene (14.38 %) and γ-terpinene (8.07 %) were observed as main constituents in C. jambhiri root oil whereas cis-linalool oxide (19.85 %), trans-linalool oxide (furanoid) (14.86 %), terpinen-4-ol (7.37 %) and limonene (4.64 %) were the major components of C. jambhiri fruit peel oil. Even though the samples were obtained from the same plant, the compositional profile of the essential oils from various plant parts differs.

Chemical Study and Antifungal Activity of the Essential Oil of the Branches of Aniba duckei Kostermans

In this present work, it was tested the linalool pattern and the essential oil extracted from the Aniba duckei Kostermans (rosewood) branches against the fungi Colletotrichum gloesporioides and Fusarium oxysporum. To do this, we extracted the essential oil by hydrodistillation and identified and quantified the components by gas chromatography coupled to mass spectrometry (GC/MS). We obtained and isolated the fungi by the routine plating method from hose and okra plants, respectively. The results of the chromatographic analysis showed that the oil had 93.60 % linalool and 3.03 % cis-linalool oxide. The essential oil and the standard inhibited mycelial growth by 100% at concentrations of 0.4 and 0.2% respectively. Therefore, the essential oil and the linalool pattern have antifungal action against the fungi demonstrated.

A decisive strategy for monofloral honey authentication using analysis of volatile compounds and pattern recognition techniques

In an effort to determine some undisputed markers of botanical origin, the volatile profile of Hellenic monofloral honeys was investigated using gas chromatography coupled to mass spectrometry (GC–MS) in combination with headspace solid phase microextraction (HS-SPME). Different varieties of honey (citrus, fir, pine, and thyme) were analyzed and semi-quantitative data of volatile compounds originating from 82 samples were subjected to multivariate analysis of variance (MANOVA).Results showed that by using only 9 volatiles [dill ether (0.012 mg kg−1), alpha-4-dimethyl-3-cyclohexene-1-acetaldehyde (0.030 mg kg−1), acetic acid ethyl ester (0.030–0.173 mg kg−1), octanoic acid ethyl ester (0.004–0.547 mg kg−1), methylanthranilate (0.030 mg kg−1), 2,2,4,6,6-pentamethyl-heptane (0.005–0.057 mg kg−1), phenylacetaldehyde (0.014–1.248 mg kg−1), cis-linalool oxide (0.007–0.010 mg kg−1), lilac aldehyde (isomer III) (0.032–0.437 mg kg−1)] honeys could be classified correctly according to botanical origin. The correct prediction rates were 93.9%, 96.8%, and 89.5%, based on the cross-validation method and using stepwise linear discriminant analysis, training and holdout partitions (k-nearest neighbors analysis), respectively. The efficiency of the discrimination model was confirmed further by application to mixed floral honeys and the use of the aforementioned consecutive pattern recognition techniques. The use of specific volatile compounds in combination with statistical tools may constitute a fast, decisive and accurate strategy for monofloral honey authentication.

A comparative analysis of the volatile components of green tea produced from various tea cultivars in China

China is a major producer and consumer of green tea and possesses numerous high-quality tea cultivars, which can be used to make green tea. However, the green teas produced from these tea cultivars vary markedly in aroma. In this study, a sensory evaluation method and headspace solid-phase microextraction combined with GC-MS were employed to determine the primary substances that contribute to the aromas and key differences in the aroma components of green tea produced from 20 tea cultivars. The results revealed 57 major volatile components, among which aldehydes, alcohols, and esters were the most prevalent. For the volatile components, the linalool, nonanal, and geraniol contents were the highest, followed by dimethyl sulfide, methyl salicylate, heptanal, and caproicacidhexneylester. The volatile components varied considerably among the green tea samples, and some contained substances with a high content that gave off unique aromas. The correlation analysis revealed significant correlations between the sensory evaluation scores and contents of linalool, geraniol, methyl salicylate, cis-linalool oxide, 3-carene, phenethyl alcohol, limonene, (Z)-3-cis-3-hexenyl isovalerate, citral, (E)-s-ocimene, alcohols, esters, and alkenes, as well as between the sensory evaluation scores and total volatile component contents. Aromatic substances with the highest odor activity values were, in descending order, linalool, capraldehyde, dimethyl sulfide, s-ionone, geraniol, nonanal, heptanal, (E)-2-nonenal, and caproicacidhexneylester. These substances were the major contributors to aroma formation in the green tea samples. The principal component analysis and orthogonal projections to the latent structure-discriminant analysis suggested that the 20 tea cultivars could be grouped into 6 categories and that the high content of linalool, nonanal, and geraniol substantially contributed to the categorization of the tea cultivars. A key variable analysis revealed that 23 volatile components played a critical role in the categorization of the tea cultivars.

Botanical Origin Authentication of Polish Phacelia Honey Using the Combination of Volatile Fraction Profiling by HS-SPME and Lipophilic Fraction Profiling by HPTLC

Eleven samples of Polish Phacelia tanacetifolia Benth., three Brassica napus and one Salix spp. honeys were characterized by melissopalynology and analysis of the compositions of their volatile fractions. Headspace solid-phase microextraction coupled with gas chromatography mass spectrometry (HS-SPME/GC–MS) using PDMS/CAR/DVB fiber was used for the isolation of low-molecular weight compounds which create a volatile fraction. To differentiate and indicate the most representative unifloral samples, chemometric techniques such as principal component analysis (PCA) and hierarchical-tree clustering (HTC) were applied to the dataset of the chromatographic fingerprints. Based on the obtained results, a unique chemical fingerprint of phacelia honey was generated. This study allows us to discriminate the botanical origin of the phacelia honeys based on the GC–MS and HPTLC analysis. In case of the GC–MS analysis trans-linalool oxide, hotrienol, cis-linalool oxide and cis-epoxylinalool were identified as a predominant compound. Additionally lipophilic fractions obtained by ultrasound-assisted extraction (UAE) and solid-phase extraction (SPE) were subjected to the HPTLC analysis. It allowed the construction of a barcode-type identifier that could be used to differentiate the honey samples even without identifying the individual components of the obtained fraction.

Identification of floral aromatic volatile compounds in 29 cultivars from four groups of Osmanthus fragrans by gas chromatography–mass spectrometry

Osmanthus fragrans Lour. is a popular aromatic ornamental plant and its flowers are used to enhance the color and fragrance of food. In this study, we analyzed the volatiles of 29 cultivars from all four groups of O. fragrans using a solid-phase microextraction (SPME) technique and gas chromatography–mass spectrometry (GC–MS). We identified 41 volatile organic components that were distributed over five different compound classes, with the majority of the volatile components being dominated by alcohols, ketones, and terpenes, which accounted for 56.6–95.06% of the total volatiles in all tested materials except ‘Zao Yingui’. In ‘Zao Yingui’, alcohols, ketones, and terpenes accounted for only 48.19% of the total volatiles. The diversity of the volatile compounds and their relative contents varied among the four groups and cultivars within each group. The major volatile compounds were α-ionone, β-ionone, 2H-β-ionone, linalool, trans-linalool oxide, cis-linalool oxide, epoxy linalool, geraniol (Z)-ocimene, and γ-decalactone in all tested cultivars, while nerol and (Z)-3-hexenyl butanoic acid ester were abundant in several cultivars. The 29 cultivars were classified into five clusters in a hierarchical cluster analysis based on their floral volatile compounds. The cultivars of different sexes (male vs. hermaphrodite) had no significant differences in the relative contents of the major volatile compounds. This study provides valuable information for understanding the chemical composition of the volatile compounds of O. fragrans flowers as well as a theoretical basis for the origin, development, and application of modern cultivars of O. fragrans.

Identification and quantification of key odorants in the world's four most famous black teas.

Keemun, Assam, Darjeeling and Ceylon black teas are honored as the world's four most famous black teas, and their excellent aroma qualities are well received by people around the world. In this study, aroma components in these four types of teas were analyzed by comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry (GC × GC-TOFMS) and gas chromatography-olfactometry (GC-O) technologies. A total of 42 aroma-active compounds were ultimately identified, especially benzeneacetaldehyde, geraniol, (Z)-3-hexen-1-yl hexanoate, trans-β-ionone, cis-linalool oxide (pyranoid), hotrienol, and methyl salicylate presented the strongest aroma strengths with pleasant scents in all tested teas. The quantification results indicated that 19 compounds including (Z)-3-hexenol, 1-octen-3-ol, linalool, phenylethyl alcohol, hexanal, benzeneacetaldehyde, limonene, heptanoic acid, (Z)-3-hexen-1-ol, acetate, benzyl alcohol, trans-linalool oxide (furanoid), hotrienol, 1-octen-3-one, 2-nonanone, (E)-2-octenal, nonanal, β-myrcene, 2-pentylfuran, and methylpyrazine were identified as the key compounds with odor activity values (OAVs) higher than 1.0 in the world's four most famous black teas. Notably, the comparison of GC-O and OAV calculation results showed that methyl salicylate (Ceylon), (E)-2-octenal (Assam), benzeneacetaldehyde (Keemun) and linalool and trans-linalool oxide (furanoid) (Darjeeling) might be the most definitive odorants in the corresponding tea categories.

Influence of Distillation Time on the Content and Composition of Essential Oils Isolated from Different Parts of Agastache astromontana ‘Pink Pop’

The chemical composition of flowers and leaves essential oils of Agastache astromontana ‘Pink Pop’ was determined by GC/MS method. The essential oils were isolated by hydrodistillation in a Clevenger-type apparatus. The following distillation times were applied in this experiment: 2, 3 and 4 hours. Linalool (23.76-24.65 %), bornyl acetate (27.22-28.61 %), camphene (5.75-7.00 %) and cis-linalool oxide (4.70-5.12 %) dominated in the essential oils isolated from the flowers. Similarly, linalool (36.48-38.01 %), bornyl acetate (15.35-17.31 %), sabinene (4.38-4.66 %) and camphene (4.17-4.52 %) were found to be the main components in the essential oils isolated from the leaves of Agastache. On the base of obtained results of the study it was proved that the distillation time did not significantly affect the essential oil content. Similarly, the part of plant had not a significant effect on the content of essential oil. However, there was a significant interaction found between the essential oil constituent and the distillation time.

Geographical differentiation of Cypriot multifloral honeys through specific volatile compounds and the use of DFA

The objective of the present work was to investigate the volatile profile of multifloral honeys collected from different regions in Cyprus, and whether these specific volatiles could serve as true markers of geographical origin. An effort to highlight differences in the volatile amounts of Cypriot honeys according to altitude of harvesting was also carried out. Aqueous solutions of honeys were subjected to headspace solid phase microextraction coupled to gas chromatography/mass spectrometry analysis (HS-SPME/GC-MS). Results showed that 25 volatile compounds of different classes were identified in all samples by 100% frequency rate. Application of multivariate analysis of variance (MANOVA) showed that 3 volatiles [1-(2-furanyl-ethanone), cis-linalool oxide, para-cymenene] recorded significant variations ( p 0.05 ) according to geographical origin. Discriminant function analysis (DFA) classified honeys according to geographical origin providing a total classification rate of 85% using the original and 70% the cross validation method based on the aforementioned volatiles. It is possible then for an analyst to characterize the geographical origin of honey, even in cases when multifloral honeys are subjected to research/analysis. Regarding the effect of altitude on Cypriot honey aroma, results showed that the lower the altitude of harvesting the richer the aroma of honeys.

Larvicidal activity of Zanthoxylum acanthopodium essential oil against the malaria mosquitoes, Anopheles anthropophagus and Anopheles sinensis

BackgroundZanthoxylum acanthopodium has insecticidal effect in Chinese traditional medicine. In this study, the essential oil from the dried Zanthoxylum plant was used as a larvicidal compound against the malaria mosquitoes, Anopheles anthropophagus and Anopheles sinensis.MethodsCompounds in the Zanthoxylum essential oil were investigated by gas chromatography and mass spectroscopy (GC–MS). The larvicidal bioassays of the whole oil, as well as the main compounds in the oil (estragole and eucalyptol) were performed using WHO method.ResultsIn total, 63 main compounds (99.32%) were found in the oils, including estragole (15.46%), eucalyptol (10.94%), β-caryophyllene (5.52%), cis-linalool oxide (3.76%), cis-limonene oxide (3.06%). A dose-dependent effect on mortality was recorded with increasing concentrations of essential oil and compounds increasing mortality of the larvae. Larvicidal bioassays revealed that 24 h LC50 of the whole essential oil was 36.00 mg/L and LC90 was 101.49 mg/L against An. anthropophagus, while LC50 was 49.02 mg/L and LC90 was 125.18 mg/L against An. sinensis. Additionally, 24 h LC50 of estragole were 38.56 and 41.67 mg/L against An. anthropophagus and An. sinensis, respectively, while the related LC90 were 95.90 and 107.89 mg/L. LC50 of eucalyptol were 42.41 and 45.49 mg/L against An. anthropophagus and An. sinensis, while the related LC90 were 114.45 and 124.95 mg/L.ConclusionThe essential oil of Z. acanthopodium and its several major compounds may have potential for use in the control of malaria mosquitoes.

Variation of the Phytochemical Constituents of Different Individual Plants in Satureja macrosiphonia Bornm (Labiatae) Growing Wild in Iran

Satureja species are commercially and medicinally important due to their high variability of essential oil components. In this study the essential oil compositions of eleven individual plants of Satureja macrosiphonia Bornm (Labiatae) from Ilam province (Iran) were characterized. The oils were isolated from flowering aerial parts of the plants by hydro-distillation method and then subjected to GC and GC-MS analyses to determine the constituents. Forty-two compounds were identified in the essential oils. The oil of S. macrosiphonia was rich in oxygenated monoterpens (ranging from 31.1 to 77.1%) and monoterpene hydrocarbons (from 14.5 to 60.7%). Limonene (0.2-20.1%), β-phellandrene (0.2-22.9%), γ-terpinene (1.0-21.1%), trans-sabinene hydrate (1.2-15.0%), cis-linalool oxide (0.4-30.5%), linalool (0.1-15.5%), cis- sabinene hydrate (0.2-24.5%), p-menth-2-en-1-ol (0.9-28.0%), borneol (0.2-8.2%), 4-terpineol (0.2-24.0%) and crvacrol (0.2-5.7%) were the major essential oil constituents. Variation of essential oil constituents found in this study represents the S. macrosiphonia as a unique chemotype in comparison to the other Satureja species.

Terpene content of wine from the aromatic grape variety ‘Irsai Oliver’ (Vitis vinifera L.) depends on maceration time

AbstractThis study deals with the determination of the content of both free and bound terpenes in berries and wine of the aromatic grapevine variety ‘Irsai Oliver’. Grapes were macerated in juice for different time intervals (viz. 0; 5; 12; 24 hours) and thereafter processed to wine. The objective was to map the dependence of some selected terpenes on the period of maceration. Using gas chromatography, some nine organic compounds were detected. Attention was paid to contents of linalool (3,7-dimethylokta-1,6-dien-3-ol), 2,6-dimetyl-3,7-octadiene-2,6-diol, hotrienol ([(5E)-3,7-dimethylocta-1,5,7-trien-3-yl] acetate), αterpineol (2-(4-Methyl-1-cyclohex-3-enyl)propan-2-ol), β-citronellol (3,7-Dimethyloct-6-en-1-ol), nerol ((Z)-3,7-dimethyl-2,6-octadien-1-ol), geraniol ((trans)-3,7-dimethyl-2,6-oktadien-1-ol) and epoxylinalool (2-(5-ethenyl-5-methyloxolan-2-yl)propan-2- ol): epoxylinalool 1 (trans-linalool oxide (furanoid) cis-linalool oxide (furanoid)) and epoxylinalool 2 (trans-linalool oxide (pyranoid) cis-linalool oxide (pyranoid)). Some basic wine parameters (alcohol, pH, sugars and total acids) were estimated as well. The terpene content in wine increased gradually with the period of maceration. The highest and the lowest amounts of terpenes were recorded after 24 hours of maceration and no maceration, respectively. The terpene glycosides content was higher than that of the aglycones. Linalool and 2,6-dimetyl-3,7-octadiene-2,6-diol were the most abundant terpenes.Based on the results of this study it is possible to conclude that the ‘Irsai Oliver’ variety of grape has a very high terpene content. To improve the aromatic profile of the wine, the grape must should be macerated for 24 hours.

Volatile organic compounds of Thai honeys produced from several floral sources by different honey bee species.

The volatile organic compounds (VOCs) of four monofloral and one multifloral of Thai honeys produced by Apis cerana, Apis dorsata and Apis mellifera were analyzed by headspace solid-phase microextraction (HS-SPME) followed by gas chromatography and mass spectrometry (GC-MS). The floral sources were longan, sunflower, coffee, wild flowers (wild) and lychee. Honey originating from longan had more VOCs than all other floral sources. Sunflower honey had the least numbers of VOCs. cis-Linalool oxide, trans-linalool oxide, ho-trienol, and furan-2,5-dicarbaldehyde were present in all the honeys studied, independent of their floral origin. Interestingly, 2-phenylacetaldehyde was detected in all honey sample except longan honey produced by A. cerana. Thirty-two VOCs were identified as possible floral markers. After validating differences in honey volatiles from different floral sources and honeybee species, the results suggest that differences in quality and quantity of honey volatiles are influenced by both floral source and honeybee species. The group of honey volatiles detected from A. cerana was completely different from those of A. mellifera and A. dorsata. VOCs could therefore be applied as chemical markers of honeys and may reflect preferences of shared floral sources amongst different honeybee species.

Chemical compositions of the essential oils of inflorescence and vegetative aerial parts of Elsholtzia blanda (Benth.) Benth. (Lamiales: Lamiaceae) from Meghalaya, North-East India

The compositional analysis of essential oil content in inflorescence and vegetative aerial parts of Elsholtzia blanda Benth. (Lamiales: Lamiaceae) growing wild in Meghalaya,North-East India were studied by GC and GC-MS. A total of 30 and 17 components representing 96.9%-98.3% and 92.6%-92.8% of the total oils from the inflorescence and vegetative aerial parts, respectively, were identified. Significant difference in composition of oils were observed between the two plant parts; 15 components detected in the oil of inflorescence were lacking in the oil of vegetative aerial parts, while two components present in the oil of vegetative aerial parts were absent in the inflorescence oil. Linalool was the major component in oils of both inflorescence (77.3%-80.2%) and vegetative aerial parts (57.9%-62.9%). Trans- linalool oxide (8.4%-9.9%), cis-linalool oxide (8.3%-9.3%) and 3,7-dimethyl-1,5-octadiene-3,7-diol (4.7%-5.1%) were however found in higher amount in the oil of vegetative aerial parts compared to inflorescence. For the first time the composition of oil in the inflorescence part has been reported in this communication while the oil from vegetative aerial parts containing linalool as the major component (44.9%) was although reported earlier but comparatively higher percentage of same was observed in vegetative aerial part (57.9%-62.9%) and inflorescence (77.3%-80.2%) in the present investigation with the plants from Meghalaya, India.

Water-soluble essential oil components of fresh flowers of Osmanthus fragrans Lour

Water-soluble essential oil components of Osmanthus fragrans fresh flowers were recovered from the hydrolate and the cold infusion, byproducts of essential oil isolation and concrete extraction, respectively. Chemical investigation was performed by gas chromatography (GC) and gas chromatography – mass spectrometry (GC-MS). As a comparative study, the Clevenger-distilled, decanted and absolute oils were also analyzed. The oil recovered from the hydrolate was mainly composed of cis-linalool oxide (furanoid) (51.4%) and trans-linalool oxide (furanoid) (37.8%). This oil presented 0.024 ± 0.004% (w/w) relative to the fresh flowers, and accounted for 25.5 ± 5.8% of the total oil (decanted + recovered). The oil recovered from the cold infusion was rich in 4-methoxyphenethyl alcohol (31.0%), 4-hydroxyphenethyl alcohol (18.0%), cis-linalool oxide (furanoid) (9.8%), cis-linalool oxide (pyranoid) (8.9%) and trans-linalool oxide (furanoid) (7.3%). This oil accounted for 0.018 ± 0.003% (w/w) of the fresh flowers. In summary, the recovered water-soluble oils are valued for being rich in compounds characteristic of O. fragrans and can find applications in the essential oil industry.

Study on essential oils from the leaves of two Vietnamese plants: Jasminum subtriplinerve C.L. Blume and Vitex quinata (Lour) F.N. Williams

The essential oil constituents of the leaves of Jasminum subtriplinerve (Oleaceae) and Vitex quinata (Verbanaceae) cultivated in Vietnam were analysed by gas chromatography – flame ionisation detector (GC–FID) and gas chromatography – mass spectrometry (GC–MS) techniques. The main constituents identified in J. subtriplinerve were mainly oxygenated monoterpenes represented by linalool (44.2%), α-terpineol (15.5%), geraniol (19.4%) and cis-linalool oxide (8.8%). The quantitative significant components of V. quinata were terpene hydrocarbons comprising of β-pinene (30.1%), β-caryophyllene (26.9%) and β-elemene (7.4%). The chemical compositions of the essential oils are being reported for the first time.

Composition of Peucedanum longifolium Waldst. & Kit. essential oil and volatiles obtained by headspace

The present study reports the chemical composition of Peucedanum longifolium essential oil and headspace (HS) volatiles obtained from the fresh aerial parts. The major contributors of the essential oil were β-elemene (24.7%), (E)-β-ocimene (11.7%), cis-linalool oxide (furanoid, 5.7%), (Z)-β-ocimene (5.6%) and α-humulene (5.2%). HS analysis of the above ground plant parts has enabled the identification of thirty-one components, representing 98.7% of the total volatiles. The main constituents were: (E)-β-ocimene (28.5%), (Z)-β-ocimene (11.9%), α-pinene (8.9%), myrcene (8.5%), p-cymene (6.4%), limonene (6.3%), sabinene (6.0%) and α-phellandrene (5.6%). Twenty-six components were in common for both the oil and HS volatiles, which is 77.3% of the oil and 97.1% of the HS sample.

Enantiomer distribution of major chiral volatile organic compounds in selected types of herbal honeys.

In this article, volatile organic compounds in 14 honey samples (rosemary, eucalyptus, orange, thyme, sage, and lavender) were identified. Volatile organic compounds were extracted using a solid phase microextraction method followed by gas chromatography connected with mass spectrometry analysis. The studied honey samples were compared based on their volatile organic compounds composition. In total, more than 180 compounds were detected in the studied samples. The detected compounds belong to various chemical classes such as terpenes, alcohols, acids, aldehydes, ketones, esters, norisoprenoids, benzene and furane derivatives, and organic compounds containing sulfur and nitrogen heteroatom. Ten chiral compounds (linalool, trans-linalool oxide, cis-linalool oxide, 4-terpineol, α-terpineol, hotrienol, and four stereoisomers of lilac aldehydes) were selected for further chiral separation.