The morphology and infraciliature of a hypotrichous ciliate collected from brackish-water biotope (salinity 5 parts per thousand) in South Korea were investigated which indicate this organism should be a new form within the genus Anteholosticha Berger, 2003. Careful morphological comparison and SSU rRNA gene sequence alignment with similar species are performed to support the validity of Anteholosticha pseudomonilata n. sp. The main diagnostic and distinguishing characteristics of the new species include: 1) 8-11 transverse cirri; 2) midventral complex composed of 10-16 pairs of zigzagging midventral cirri, extending posteriorly to slightly ahead of pretransverse cirri; 3) cortical granules colourless and pigment-like, 0.5 mu m across, longitudinally arranged in more or less short rows on whole cortex except along dorsal kineties and cirral rows; and 4) 8-12 macronuclear nodules located left of midline.