The endosperm of the cereal grasses has been the subject of numerous investigations directed from many different points of view, but certain phases of its development, involving the differentiation of its tissues, the accumulation of food material, and the relations between the endosperm and the embryo, have not been thoroughly studied. The application of the principle of the alternation of generations to the higher plants during the latter part of the past century gave to the endosperm a new morphological meaning, which was modified and made more interesting by the discovery of and by the studies on xenia at the very close of the century. In most studies of development, however, the embryo has been the structure emphasized, and the chief interest in the endosperm has been its r6le as a nurse tissue for the embryo. From the work that has been done on various grasses it is evident that there is considerable uniformity in the development of the endosperm. The polar nuclei remain in contact with each other, without fusing, until the time of fecundation. The union of a sperm with them has been observed in Zea (9, I2, I7) and in Triticum (ii), and all circumstantial evidences indicate that double fecundation regularly occurs in other species. After fecundation there is a period of rapid division of free nuclei, with a migration of nuclei toward the antipodal end of the endosperm cell (Randolph, I3). These nuclei are located in a thin wall layer of cytoplasm, and the center of the cell is a large vacuole (P1. XXVIII, fig. I3). The formation of cell walls apparently begins in the peripheral region, for a stage of development is soon to be seen in which the endosperm is a hollow mass of tissue surrounding a central cavity (text figs. I, 2). The steps of development preceding this stage have not been investigated in detail, but it is reasonable to assume that they are similar to those of corresponding stages in the development of the endosperm of typical angiosperms. It would probably be better to regard this hollow endosperm as a cellular mass having a large, multinucleate cell in the middle. This cavity is soon eliminated by further nuclear division and wall formation (text fig. 3 and P1. XXVIII, fig. I4). Of the course of development of the endosperm from this time onward we have only fragmentary information drawn from studies of different grasses. These studies long ago indicated considerable differentiation in the mature endosperm of the cereals. Publication 46 of the Waterman Institute, Indiana University. 37I
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