Purpose: In order to screen for invasive mosquito species and associated viruses, we performed a field survey for mosquitoes in the Black Sea region of Anatolia where Aedes aegypti and Aedes albopictus were previously recorded. Methods & Materials: Mosquitoes were collected from 31 sites in Artvin, Trabzon and Rize provinces during 2016-2017. The specimens were identified morphologically and pooled according to collection site and species. Selected specimens were processed for DNA barcoding via cytochrome oxidase I amplification and sequencing, for confirmation of the morphological species identification. Virus screening was carried out using polymerase chain reaction (PCR) assays targeting alpha and flaviviruses, as well as recently-described novel rhabdovirus Merida-like virus Turkey (MERDLVT), followed by sequencing for characterization. Results: A total of 756 mosquitoes that comprise Ae. albopictus (675, 89,2%), Ae. aegypti (61, 8,1%) and Culex pipiens sensu lato (20, 2,6%) were collected and grouped in 65 pools. No amplification was observed in any pool via generic alphavirus PCR. Generic flavivirus PCR was reactive in 7 and 8 pools collected in 2016 and 2017, respectively. Cell fusing agent virus (CFAV) sequences were characterized in 4 pools (6,1%) of Ae. albopictus (n = 2) and Ae. aegypti (n = 2), collected in 2016. Aedes flavivirus (AFEV) sequences were characterized in 6 pools (9,2%) of Ae. albopictus (n = 5) and and Ae. aegypti (n = 1), collected in 2016 (n = 3) and 2017 (n = 3). Sequences of West Nile virus (WNV) was detected in 5 pools (7,6%) of Ae. albopictus (n = 4) and and Cx. pipiens s.l. (n = 1), collected in 2017. In phylogenetic analyses, the WNV sequences clustered with local and global lineage 1 clade 1a strains. Moreover, partial L and N gene sequences of MERDLVT were identified in the Cx. pipiens s.l. pool, coinfected with WNV. Conclusion: This is the initial detection of WNV and MERDLVT in field-collected mosquitoes from the Black Sea region. Although no alphavirus sequence could be demonstrated, presence of Ae. albopictus and Ae. aegypti indicates ongoing risk for potential spread.