Lighter Research Articles

Effects of 15-deoxy-△12,14-prostagliandxin J2 on the expression of type I collagen in hypertrophic scar of rabbit ears

Objective To investigate the effect of 15d-PGJ2 on the expression of type Ⅰ collagen in hypertrophic scar of rabbit ears, and the possibility of hypertrophic scar treated by 15d-PGJ2. Methods Eighteen New Zealand white rabbits were used to establish the hypertrophic scar models on the rabbit ears. The wounds were created as follows: 2 cm × 3 cm wounds with total skin loses on the ventral side, 2wounds for each ear, total 72 wounds. The wounds were randomly divided into the 15d-PGJ2 treatment group and NS control group. A total of 20 μl 15d-PGJ2 or NS was injected into the ear scar once a day for 7 days. At the 14th and 21st day after the injection, 18 scars in each group were harvested. The expression levels of type Ⅰ collagen was detected by immunohistochemistry, fluorescence quantitative polymerase chain reaction (PCR) and Western blotting. Results Compared with the NS control group, the 15d-PGJ2-treated scars appeared to be smaller, softer, flatter and lighter in color. The expression of type Ⅰcollagen was mainly distributed in matrix, fibroblast cytoplasma and the vascular wall. The mRNA and protein levels of type Ⅰ collagen were significantly decreased in the 15d-PGJ2-treated group as compared with those in the NS control group at different time points (P < 0. 05). Conclusion 15d-PGJ2, the ligand of PPAR-γ, can reduce the expression of type Ⅰ collagen in hypertrophic scar of rabbit ears and plays an important role in the prevention and treatment of hypertrophic scar. It offers a new way for treating the hypertrophic scar clinically. Key words: Hypertrophic scar; PPAR-γ; Collagen

IABSE-IASS Symposium London, September 20–23, 2011: Taller, Longer, Lighter

IABSE-IASS Symposium London, September 20–23, 2011: Taller, Longer, Lighter

Effects of sevoflurane versus propofol anesthesia on inflammatory response and pulmonary function during perioperative period in patients undergoing lung cancer resection

Objective To compare the effects of sevoflurane and propofol anesthesia on inflammatory response and pulmonary function during perioperative period in patients undergoing lung cancer resection．Methods Thirty ASA Ⅰ or Ⅱ patients(23 male，7 female) aged 41-64 yr having a body weisht index of 22-30 kg/m2 undegoing elective left lower lobe resection were randomly divided into 2 groups(n=15 each)：sevoflurane group (group S) and propofol group(group P)．Anesthesia was induced with 6％-8％ sevoflurane or propofol 2 mg/kg and fentanyl 4-6 μg/kg．Intubation with double lumen catheter was facilitated with vecuroniunl 0．1 mg/kg． Anesthesia was maintained with 1％-3％ sevoflurane/propofol infusion(6-10 mg·kg-1·h-1)and intermittent iv boluses of fentanyl and vecuronium．Radial artery was cannulated．Swan-Ganz catheter was placed via right internal jugular vein．The patients were mechanically ventilated．During one lung ventilation(OLV)I：E and VT were adjusted to maintain airway pressure <30 cm H2O．Arlerial and mixed venous blood samples were collected for blood gas analysis before induction of anesthesia(T0)，before OLV(T1)，at the end of OLV(T2)，when the chest was closed(T3) and at 24 h after operation (T4)．PA-aO2，Qs/Qt and respiratory index(RI，PA-aO2/PaO2) were calculated. Serum matrix metallo-proteinase-9 (MMP-9) and MDA were measured at T0, T3 and T4. Dynamic lung compliance (Cd) was calculated at T1-3 .Results PA-aO2, RI and Qs/Qt at T1-3 and serum MMP-9 and MDA concentrations at T3 were significantly increased as compared with the baseline values at T0 in both groups. In group S, Cd was significantly lower at T3 than at T1.PA-AO2, and serum MMP-9 and MDA concentrations at T3, RI at T2,3 and Qs/Qt at T1-3 were significantly higher in group S than in group P. Conclusion The inflammatory response is lower and the injury to pulmonary function is lighter during propofol anesthesia than sevoflurane anesthesia in patients undergoing lung cancer resection. Key words: Anesthetics, inhalation; Propofol; Inflammation; Respiratory function tests; Lung neoplasms

Quality Characteristics of Erythritol Icebox Cookies

Sucrose is one of the principal ingredients of cookies. But a sucrose-rich diet is associated with lipogenesis and obesity. Erythritol is a noncariogenic, nonglycemic and low calorie sweetener that is safe for diabetics. The study explored the feasibility of utilizing erythritol in icebox cookie making. Icebox cookies were prepared with 25%, 50%, 75% and 100% replacement of sucrose with erythritol, respectively, and evaluated for quality indices. The Hunter L value increased with the increased erythritol level, while the product texture showed a reverse effect. No difference was found in the moisture, water activity, protein, fat, ash, and dimensions of icebox cookies prepared with sucrose or erythritol. The erythritol content (dry basis) in icebox cookies before and after baking was not significantly different. In descriptive analysis, the surface color, sweetness, and hardness of icebox cookies became lighter, less sweet, and softer, respectively, as the erythritol level increased and the sucrose level decreased. The 50%, 75%, and 100% erythritol icebox cookies had a cooling sensation. The moistness of icebox cookies showed no difference among all samples. On a seven-point hedonic test, the degree of surface color, sweetness, hardness, flavor and overall liking of icebox cookies prepared with 0%, 25% and 50% replacement of sucrose with erythritol were moderately acceptable (5.1 to 5.6, 5=like slightly). However, the lower liking degree of sensory qualities of 100% erythritol icebox cookies was observed. Overall, a partial replacement of sucrose with erythritol in icebox cookie making is feasible.

Clinical research about postoperative of colorectal cancer without gastric tube

Objective To probe into clinical results on coloreetal cancer operation with and without home tube. Methods Two hundred colorectal cancer sufferers were randomly divided into 94 cases with home tube operation and 106 cases without home tube operation,then gastrointestinal tract function recovery time, early feeding & get out of bed time and complications among the two groups were analyzed. Results After colorectal cancer operation without home tube, the patients'anal discharge, feeding & get out of bed time were earlier than patients with home tube operation (P ＜ 0. 01 );patients without home tube operation had lower rate of sore throat, nausea & vomiting and lung infection than patients with home tube operation. Conclusions It is not necessary for regular home tube prior to colorectal cancer operation, operation without home tube would have less trauma, lighter suffering, fewer complications and faster postoperative recovery. Key words: Home tube; Colorectal cancer operation

Role of electrical stimulation of S_3 nerve root in improvement of intestinal mucosal barrier function after acute complete spinal cord injury in rabbits

Objective To investigate the effect of electrical stimulation of S_3 nerve root on improvement of intestinal mucosal barrier function in rabbits with acute complete spinal cord injury. Methods Model of paraplegia was built by injuring spinal cord in rabbits. Then, the rabbits with electrical stimulation of S_3 nerve root were set as experimental group and those without set as control group. Normal rabbits were set as normal group. Under aseptic condition, portal vein blood was collected for quantitative determination of endotoxin and bacterial culture ; and liver, spleen and mesenteric lymph nodes were collected for bacterial culture and strain identification. Liver, spleen, mesenteric lymph nodes and small intestines were collected from experimental group and control group for pathological HE staining; while small intestine were observed by light and electron microscopes. Results In control group, the intestinal mueosal barrier and the other organs were destroyed obviously, with higher level of Serum endotoxin and higher rate intestinal flora translocation than that in experimental group and normal group. In the experimental group, the electrical stimulation of S_3 nerve root could improve motility of the denervated intestine, with more defecation content, less destruction of the intestinal mucosa and lighter other organ damage compared with control group, serum endotoxin level was significantly reduced compared with control group but showed no statistical difference compared with normal group, with obvious decrease of bacterial translocation rate. Conclusions After spinal cord injury in rabbits, electrical stimulation of S_3 nerve root can facilitate intestinal tract motility, improve intestinal mucosal barrier function and hence alleviate endotoxemia and intestinal bacterial translocation, as is beneficial to reducing SIRS and MOBS. Key words: Spinal cord injuries; Paraplegia; Intestinal mucosal barrier function; Electric stimulation therapy

Effect of 15-deoxy-△~(12,14)-prostagliandxin J2 on hypertrophic scar in rabbit ear

Objective To investigate the effect of 15d-PGJ2 on the expression of collagen type,CTGF and a-SMA in the hypertrophic scar in the rabbit ear,and the possibility of hypertrophic scar treated by 15d-PGJ2.Methods 18 New Zealand white rabbits were used to establish a hypertrophic scar model on the rabbit ear.The wounds were established as follows:2 cm × 3 cm wounds with total skin loses on the ventral side,2 wounds for each ear,totally 72 wounds.The wounds were randomly divided into the 15d-PGJ2 treatment group and NS control group.20μl 15d-PGJ2 or NS was injected into the ear scar once a day for 7 days.At 7,14 and 21 days after the injection,12 scars of each group were harvested.The expression of collagen type Ⅰ,CTGF and a-SMA was detected by immunohistochemical method.Results Excessive dermal scars on rabbit ears that were similar to human hypertrophic scar appeared in the two groups.Compared with the NS-treated scars group,the 15d-PGJ2-treated scars appeared to be smaller,softer,flatter and lighter in color.The expression of collagen type Ⅰ,CTGF and a-SMA in the 15d-PGJ2 group was significantly decreased as compared with that in the control group at different time points(P＜0.05).Conclusion 15d-PGJ2,the ligand of PPAR-r,can reduce the expression of collagen type Ⅰ,CTGF and a-SMA of hypertrophic scar in the rabbit ears and plays an important role in the prevention and treatment of hypertrophic scar.It may provide a new approach for the treatment of hypertrophic scar in clinical setting. Key words: Hypertrophic scar; Peroxisome proliferator activated receptor-r; PPAR-r; Collagen type Ⅰ; Connective tissue growth factor; CTGF; a-Smooth muscle actin; a-SMA

The realize of the both point anesthetic method of the epidural space used to negativeway hysterectomy

Objective To used to negative way hysterectomy with the both paint negative way method of the epidural space. Methods 40 patients of the negative way anesthetic hysterectomy, all were used to the both point anesthetic method of the epidural space, the small pipe was towards the head at the L1～2 space and the small pipe was towards the tail at the L3～4 space. Results The myometrium uterus ligaments and the cervix were good limp. It is merrier of the topical anesthetic has keeped longer time and the dragging pain has lighter and lesser effect in the operation. Conclusion It has certainly worth of the clinical used to about the both point anesthetic method of the epiduralspace used to negativeway hysterctomy. Key words: Epiduralspace bothpain; Negativeway; Hysterectomy

Antimicrobiai bioceramic combined with chitosan and ceftriaxone sodium:Preparation and its in vitro and in vivo study

Objective To synthesize antimicrobial bioceramic using chitosan and calcium phosphate cements mixed with ceftriaxone sodium. Methods The bioceramic was synthesized through the hardening of chitosan liquid combined with calcium phosphate cements and cefiriaxone sodium.The released ceftriaxone sodium was studied according to the linear equation between UV-VIS absorbance to concentration.The in vitro bactefiostatic effect of the chosen bioceramic was investigated via the microbiological method.The model of rats'contaminated bone defect were deployed to study the antimicrobial performance of the bioceramic. Results The best synthesis condition was chosen at:0.1g calcium phosphate cements and 10.4 mg ceftriaxone sodium combined with 2.4 ml hardening liquid C.then keeping the mixture at 60℃ and 100%humidity for 24 h.In vitro release of the resulting antimicrobial bioceramic remained stable,while that of ceftriaxone sodium lasted for a week,higher than the minimal inhibitory concentration(MIC) of Staphylococcus aureus.As proved by the WBC number and tissue sectioning,a lighter inflammatory response of treatment group was observed as compared with the control group. Conclusion The antimicrobial bioceramic combined with chitosan and ceftriaxone sodium shows promising antimicrobial performance. Key words: Chitosan; Ceftriaxone sodium; Bioceramics; BMP; Osteogenesis

Effectiveness and safety of two long-pulsed lasers for hair removal in different colour of skin

Objective To evaluate the effectiveness and safety of long-pulsed laser for hair removal in different colour of skin. Methods Hair removal was performed in 607 hirsute patients with two long-pulsed lasers. Results Although the effectiveness rates of hair removal in upper lip and full bread were not different, their complications were different by using two long-pulsed lasers. Also there were signifi-cant decreases of effectiveness and complication in the lighter skin and thc darker skin (P<0.01). Con-clusion The 755 nm wavelength has more effectiveness and lower complication in the lighter skin. How-ever, the 1 064 nm wavelength has more effectiveness and lower complication in the darker skin. Key words: Laser; Hair removal; Colour of skin

Vertical Vibration of Composite Floor by Neural Network Analysis

Composite steel concrete composite floor used mainly in the commercial and office building has lighter weight and is considered very economical. The light weight natures of this type of floor causes some vibration problems when it is subjected to loadings such as human footstep loading. The dynamic characteristics of these floors are dependent on several parameters such as joist span, girder span, slab thickness and etc. In this article, using the neural network analysis, the vertical vibration of the composite floor system subjected to footstep loading is investigated. The parameters effecting on the overall vibrational characteristics is recognized. Numbers of practical cases subjected to different loading were analyzed. Finally with some of the guidelines provided from other researchers and the results obtained from this study, the vertical floor vibration induced by footstep loading can be checked and the design equations and the guidelines required for such phenomenon can be controlled.

The suppressing effect of Shenkang 2 on HPA axis of rats model

Objective To observe the inhibition effect of Shenkang 2 on rats'HPA axis,and provide theoretical basis for clinical application of steroid withdrawal and maintenance in the treatment phase.Methods To built the HPA axis suppression rat model by using high-dose glucocorticoids,when the hormone reduced to low-dose,traditional Chinese medicine Shenkang 2 were administrated.Serum ACTH,cortisol levels,adrenal index,adrenal pathological differences were observed before and after the treatment of Shenkang 2 among the treatment group,hormone control group and normal group.Results Serum ACTH,cortisol and adrenal index slowly recovered after hormone withdrawal in beth treatment group and the hormone control group,serum ACTH,cortisol and adrenal index in the treatment group rebounded faster than that in the control group,the atrophy of fascicular zone of adrenal cortex and cell disarrangement was lighter in the treatment group than that in the control group.Conclusions Shenkang 2 can improve the suppression effect of glucocorticoid-induced HPA axis suppression. Key words: Shenkang 2; Hypothalamus-pituitary-adrenal axis; Adrenocorticotropic hormone; Cortisol

Peropective comparison of hydroxyapatite orbital implant between primary and second drilling procedure

Objective To evaluate the clinical effect of the procedure that involves drilling and inserting a motility peg at the time of hydroxyapatite orbital implant.Methods 89 patients who needed hydroxyapatite orbital implant were divided into groups A and B.The patients in group A were drilled and inserted a motility titanium peg when hydroxyapatite implantation.The patients in group B were drilled and inserted a motility titanium peg 6 months after hydroxyapatite implantation.Data were recorded.including the rate of postoperative pain and conjunctive edema,postoperative facial appearance,motility of the artifieial eye,the rate of hydroxyapatite exposure,excursion and extrusion of titanium peg and other complications.Results The mobility of the prosthesis was 44.97%in group A,and 45.34%in group B.There was no difference in appearance and the motility of their artificial eye between the two groups.The rate of excursion of titanium peg was 4.2% in group A and 7.3%in group B and no statistical difference was found between the two groups.There were no prosthesis exposure,extrusion of titanium peg,secondary infeetion and other complication in one-year follow-up.There were no difference in the rate of postoperative pain and eonjunctive edema in first operative stage.The postoperative reaction in group A was lighter than that in group B in secondary operanve stage.Conclusion The hydroxyapatite orbital implant primary procedure drilling is simple,safe,convenient and effective.It is worth to recommend that procedure that involves drilling and inserting a motility peg at the time of hydroxyapatite orbitaI primary implant. Key words: Hydroxyapatite; Eye,Artificial; Orbital-Implants; Titanium peg

Effect of craniotomy on blood-brain barrier in rats

Objective To investigate whether the macromolecular materials could enter cerebrospinal fluid and brain tissues in craniotomy with incision or non-incision of dura and arachnoid. Methods Adult male SD rats were randomly divided into three groups according to the random number table. The dura and arachnoid of rats in group A were cut open during craniotomy after general anesthesia; epidural craniotomy was done in rats in group B after general anesthesia; rats in group C (control group) were only generally anesthetized. All the rats were injected with Evans blue, a tracer used to detect the results, half an hour before each time point (1,3, 6, 12, 24, 72 hours and 1 week) via vein. The rats were executed at each time point to obtain the specimens of brain. The content of Evans blue in brain tissue was measured by fluorescence spectrophotometer for statistical analysis. The water content in the brain tissue was measured in a part of rats selected in groups A and B preoperatively and at postoperative 3 and 27 hours. Results It was found that some regions of the brain tissue were stained light blue in group A at 1,3, 6 and 24 hours. The blue was much lighter in brain tissue obtained at 72 hours in group A, and no blue stained at 1 week in group A . The contents of Evans blue in the brain tissues of rats in group A at 1,3, 6, 12, 24, 72 hours and 1 week were (18.07±1.25) μg/ml, (36.21±0.78) μg/ml, (25.73±1.14) μg/ml, (16.53±0.84) μg/ml, (23.34±1.91) μg/ml, (43.34±2.25) μg/ml and (25.27±1.88)μg/ml respectively, which were significantly higher than (3.15±0.45)μg/ml, (3.36±0.33)μg/ml, (2.98±0.54)μg/ml, (3.47±0.55)μg/ml, (3.54±0.37) μg/ml, (2.88± 0.42) μg/ml and (2.85±0.22) μg/ml respectively in group B and (2.97±0.37)μg/ml in group C (P 0.05). Conclusion After craniotomy with incision of dura and arachnoid, some macromolecular materials can enter the subarachnoid space and the brain parenehyma through blood-brain barrier of the wound of the scalp if the dura is sutured loosely. Key words: Neurosurgical procedures; Blood-brain barrier; Evans blue; Craniotomy

Fly-by-wireless set for take off

Planes could be made significantly lighter through replacing wired systems with wireless monitors – but the reliability of wire-free craft has yet to be proven

Effects of fraction of inspired oxygen on nuclear factor-κB of lung tissue in rabbit one-lung ventilation model

Objective To study the effects of fraction of inspired oxygen on nuclear factor-κB (NF-κB) of lung tissue in rabbit one-lung ventilation(OLV) models.Methods 12 white Japanese rabbits were randomly divided into 2 groups(n=6).Bronchial intubation was performed with artificial double-lumen tube,and right OLV was conducted for 2 h and then tollowed tow-lung ventilation(TLV) for 1 h.Fraction of inspired oxygen was set as 1.0(group A) or 0.6(group B).NF-κB in lung tissue was detected,and arterial blood gases were analyzed before OLV,at 30 min of OLV and 30 min after TLV reversion.Pathology of lung tissue was examined and wet/dry(W/D) of lung weight was measured.Results After TLV restore,the oxygenation index was higher and lower than 300 in group B and group A,respectively.The W/D,the activation and the level of NF-κB in left lung tissue was less in group B than in group A (P＜0.01),and pathological change in left lung tissue was lighter in group B than in group A.Conclusion OLV with 60% oxygen may attenuate lung injury by decreasing the activation and the level of NF-κB in lung tissue. Key words: One-lung ventilation; Nuclear factor-κB; Fraction of inspired oxygen; Lung injury; Rabbit

Clinical features analysis of 1240 tinnitus cases

To discuss the relationship between tinnitus and deafness, the clinical features and influencing factors in tinnitus patients. Data with previous medical history, clinical manifestation and audiologic examination results, etc. of tinnitus patients were collected to find out clinical features of tinnitus. The relationship between various factors and tinnitus was therefore established. Average age of developing tinnitus syndrome was 53.2 +/- 0.9. Patients with lighter physical and mental work were the largest proportion (39.5%). Acute onset of tinnitus patients was more severe than that of chronicle (P < 0.01). Most of tinnitus melody was 8000 Hz (22.9%). Loudness of tinnitus concentrated in 5 - 10 dBSL. There was no severity of distinction between different patients with different tinnitus melody or loudness (P > 0.05). There were 75.6% patients showing sensorineural deafness. When the melody in the ears was high-frequency, hearing loss for the majority of patients was at high frequency region. Meanwhile, for the low frequency, speech frequency, hearing loss was also at the corresponding frequency region. There were 89.6% patients showing psychological adverse reactions, including irritability (83.8%), insomnia (63.7%), and inability to concentrate (30.3%). The relationship between tinnitus and deafness are inseparable. So we should carry out periodically hearing testing to tinnitus patients with normal hearing or with high-risk population (45 years age to nearly 50 years old), in order to early diagnosis and intervention. Psychological adverse reactions are the main clinical features of tinnitus. It is important for the treatment of tinnitus to correct adverse psychological state.

Correlation between major histocompatibility complex class I-related chain A gene alleles and graft rejection in small intestine, liver and kidney transplantation

To investigate the correlation between major histocompatibility complex (MHC) class I chain-related gene A (MICA) gene alleles matching rates and graft rejection in small intestine, liver and kidney transplantation. Genome DNA were extracted from blood samples or pathological sections collected from donors and recipients of living-related transplantation, included 4 cases of small bowel transplantation, 5 cases of liver transplantation and 6 cases of kidney transplantation. The correlation between MICA alleles matching rates and acute graft rejection was analyzed following 13 MICA alleles determination by polymerase chain reaction based on sequence-specific primers (PCR-SSP). HLA zygosity of all donors and recipients was confirmed to be half-matching. The recipients displaying higher matching rates of MICA alleles with donors showed lighter clinical and pathological rejection and longer survival time. On the contrary, recipients with lower matching rates of MICA alleles with donors showed severer clinical and pathological rejection and shorter survival time relatively. Matching rates of MICA alleles has negative relevance to acute rejection, and positive relevance to survival time of recipients in small bowel, liver, and kidney transplantation.

Protective effects of L-carnitine upon testicular ischemia-reperfusion damage in rats

To investigate the protective effects of L-carnitine upon testicular ischemia-reperfusion injury in rats. Sprague-Dawley rats were divided into 3 groups (n = 10). In those animals undergoing unilateral testicular torsion, right testes were rotated 720 degrees for 2 h. Sham operated group served as a control group. Torsion group underwent 2 h torsion and saline was injected intraperitoneally at 30 min pre-detorsion. Treatment group underwent similar torsion but L-carnitine (500 mg/kg) was infused intraoperatively. The right testes of 5 animals in each group were excised after 4 h reperfusion for measuring the levels of malondialdehyde (MDA) and heat shock protein 70 (HSP70), evaluation of activities of antioxidant enzyme including superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx). Histopathological changes and germ cell apoptosis indices (AI) were determined at 24 h post-detorsion in right testes of the remaining 5 animals in each group. The mean number of apoptotic nuclei per tubule cross section and the malondialdehyde level were significantly lower in treatment group as compared with torsion group [AI( 6.87 +/- 2.47) vs (17.13 +/- 3.56), MDA (160 +/- 15) vs (199 +/- 15) nmol/g]. Activities of antioxidant enzyme and the level of HSP70 were significantly higher in treatment group than those in torsion group [SOD (1638 +/- 153) vs (1078 158) U/g, CAT (317 +/- 28) vs (188 +/- 33) U/g, GPx (667 +/- 94) vs (311 +/- 65) U/g, HSP70 (0.87 +/- 0.13) vs (0.25 +/- 0.04)]. The pathological damage of testes in the treatment group was lighter than that in the torsion group (all P < 0.05). The administration of L-carnitine exerts a beneficial effect upon testicular ischemia-reperfusion injury. This effect may be achieved through an induced expression of HSP70.

Effects of L-carnosine in preventing and treating rat cataract induced by sodium selenite

To explore the effect of L-carnosine in preventing and treating rat cataract induced by sodium selenite. This was an experimental study. Cataract was induced in the rats by sodium selenite. Rats were divided into 3 groups and each group has 3 ones. L-carnosine eye drops (50 g/L or 20 g/L ) and 0.9% normal saline were respectively instilled to the rat eye for 3 weeks and examined. L-carnosine was added to the medium of cultured rat cataract lens at 1.00, 0.10 and 0.01 g/L for 1 week and then examined. Cataract lens were studied by using two-dimensional electrophoresis. One week after instillation of L-carnosine, the scores of rat cataract were 2.22 +/- 0.65, 2.39 +/- 0.98 and 2.83 +/- 0.38 in 50 g/L, 20 g/L L-carnosine and control groups, respectively. The lesion in 50 g/L L-carnosine group was lighter than that of the control group. There was significant difference between these two groups (P = 0.013). On the 2nd and 3rd weeks, there was no difference among these three groups. After 1 week for culturing cataract lens, there was no difference between these three groups. The results of two-dimensional electrophoresis showed that the protein number was 182 and 161 in the L-carnosine and control groups, respectively. High molecular weight protein decreased and low molecular weight protein increased in L-carnosine group. L-carnosine at 50 g/L could restrain the development of early stage rat cataract. L-carnosine could modulate the rat lens protein in vitro, but could not affect lens cataract scores.