Cibacron Blue F3GA Attachment Research Articles

MAGNETIC DYE-AFFINITY BEADS FOR HUMAN SERUM ALBUMIN PURIFICATION

Cibacron Blue F3GA was covalently attached onto magnetic poly(vinyl alcohol) (mPVAL) beads (100–150 μm in diameter) for human serum albumin (HSA) adsorption from human plasma. Despite low nonspecific adsorption of HSA on mPVAL beads, Cibacron Blue F3GA attachment significantly increased the HSA adsorption. The maximum HSA adsorption was observed at pH 5.0. Higher HSA adsorption was observed from human plasma. Desorption of HSA from mPVAL beads was achieved by medium containing 1.0 M KSCN at pH 8.0. To test the efficiency of albumin adsorption from human serum, before and after albumin adsorption was demonstrated with sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analyses. HSA molecules could be reversibly adsorbed and desorbed 10 times with the magnetic beads without noticeable loss in their HSA adsorption capacity.

THE PREPARATION OF CIBACRON BLUE F3GA ATTACHED CHITOSAN MICROSPHERES AND THEIR ADSORPTION PROPERTIES FOR HUMAN SERUM ALBUMIN

A series of aminated chitosan microspheres were prepared from chitosan,and then an affinity dye-ligand,Cibacron Blue F3GA,was covalently coupled.The maximum attachment of cibacron blue F3GA was 494 μmol/g.Albumin adsorption onto cibacron blue F3GA-attached chitosan microspheres was investigated.The human serum albumin (HSA) equilibrium adsorption capacity was 79.8 mg/g (wet beads).The adsorption equilibrium isotherms appeared to follow a typical Langmuir isotherm.The highest desorption ratio (100%) was achieved by using an eluent of 1.0 mol/L NaSCN (pH 8.0).The cibacron blue F3GA-attached chitosan microspheres could be reused without significant decreases in the adsorption capacities.

Bovine serum albumin (BSA) adsorption with Cibacron Blue F3GA attached chitosan microspheres

Affinity dye-ligand, Cibacron Blue F3GA, was covalently coupled with aminated chitosan microspheres via a nucleophilic reaction. The maximum attachment of Cibacron Blue F3GA was 494 μmol/g. Albumin adsorption onto both unmodified chitosan (CS) microspheres and Cibacron Blue F3GA-attached chitosan (CBF-CS) microspheres was investigated batchwise. The bovine serum albumin (BSA) adsorption capacity of the CBF-CS significantly increased (95.2 mg/g dry bead) compared with the adsorption by CS (12.7 mg/g dry bead). The adsorption equilibrium isotherms appeared to follow a typical Langmuir isotherm. The maximum adsorption capacity ( q m) of the CBF-CS microspheres for BSA was 108.7 mg/g. The highest desorption ratio (over 99%) was achieved by using 1.0 M NaSCN (pH 8.0). The CBF-CS microspheres could be reused without significant decreases in the adsorption capacities.

Affinity adsorption of recombinant human interferon-α on a porous dye-affinity adsorbent

Interferons are potent biologically active proteins synthesized and secreted by somatic cells of all mammalian species. Dye-affinity adsorption is increasingly used for protein separation. Hollow fibers have several advantages as adsorbents in comparison to conventional bead supports because they are not compressible and they eliminate internal diffusion limitations. The aim of this study was to explore in detail the performance of polyamide hollow fibers to which Cibacron Blue F3GA was attached for adsorption of recombinant interferon-α (rHuIFN-α). The hollow fiber was characterized by scanning electron microscopy. These dye-carrying hollow fibers (35.8 μmol/g) were used in the rHuIFN-α adsorption-elution studies. The effects of initial concentration of rHuIFN-α, medium pH, ionic strength and temperature on the adsorption efficiency of dye-attached hollow fibers were studied in a batch system. The non-specific adsorption of rHuIFN-α on the hollow fibers was 1.2 mg/g. Cibacron Blue F3GA attachment significantly increased the rHuIFN-α adsorption up to 99.8 mg/g. Significant amount of the adsorbed rHuIFN-α (up to 94.8%) was eluted in 1 h in the elution medium containing 1.0 M NaCl. In order to determine the effects of adsorption conditions on possible conformational changes of rHuIFN-α structure, fluorescence spectrophotometry was employed. We resulted that dye-affinity hollow fibers can be applied for rHuIFN-α adsorption without causing any significant conformational changes. Repeated adsorption/elution processes showed that these dye-attached hollow fibers are suitable for rHuIFN-α adsorption.

Affinity adsorption of recombinant human interferon-$alpha; on a porous dye-affinity adsorbent

Interferons are potent biologically active proteins synthesized and secreted by somatic cells of all mammalian species. Dye-affinity adsorption is increasingly used for protein separation. Hollow fibers have several advantages as adsorbents in comparison to conventional bead supports because they are not compressible and they eliminate internal diffusion limitations. The aim of this study was to explore in detail the performance of polyamide hollow fibers to which Cibacron Blue F3GA was attached for adsorption of recombinant interferon-α (rHuIFN-α). The hollow fiber was characterized by scanning electron microscopy. These dye-carrying hollow fibers (35.8 μmol/g) were used in the rHuIFN-α adsorption-elution studies. The effects of initial concentration of rHuIFN-α, medium pH, ionic strength and temperature on the adsorption efficiency of dye-attached hollow fibers were studied in a batch system. The non-specific adsorption of rHuIFN-α on the hollow fibers was 1.2 mg/g. Cibacron Blue F3GA attachment significantly increased the rHuIFN-α adsorption up to 99.8 mg/g. Significant amount of the adsorbed rHuIFN-α (up to 94.8%) was eluted in 1 h in the elution medium containing 1.0 M NaCl. In order to determine the effects of adsorption conditions on possible conformational changes of rHuIFN-α structure, fluorescence spectrophotometry was employed. We resulted that dye-affinity hollow fibers can be applied for rHuIFN-α adsorption without causing any significant conformational changes. Repeated adsorption/elution processes showed that these dye-attached hollow fibers are suitable for rHuIFN-α adsorption.

Cibacron Blue F3GA‐attached magnetic poly(2‐hydroxyethyl methacrylate) beads for human serum albumin adsorption

AbstractAn affinity dye ligand, Cibacron Blue F3GA, was covalently attached onto magnetic poly(2‐hydroxyethyl methacrylate) (mPHEMA) beads for human serum albumin (HSA) adsorption from both aqueous solutions and human plasma. The mPHEMA beads, in the size range of 80 to 120 µm, were prepared by a modified suspension technique. Cibacron Blue F3GA molecules were incorporated on to the mPHEMA beads. The maximum amount of Cibacron Blue F3GA attachment was obtained as 68.3 µmol g−1. HSA adsorption onto unmodified and Cibacron Blue F3GA‐attached mPHEMA beads was investigated batchwise. The non‐specific adsorption of HSA was very low (1.8 mg g−1). Cibacron Blue F3GA attachment onto the beads significantly increased the HSA adsorption (94.5 mg g−1). The maximum HSA adsorption was observed at pH 5.0. Higher HSA adsorption was observed from human plasma (138.3 mg HSA g−1). Desorption of HSA from Cibacron Blue F3GA‐attached mPHEMA beads was obtained by using 0.1 M Tris/HCl buffer containing 0.5 M NaSCN. High desorption ratios (up to 98% of the adsorbed HSA) were observed. It was possible to re‐use Cibacron Blue F3GA‐attached mPHEMA beads without any significant decreases in their adsorption capacities. Copyright © 2004 Society of Chemical Industry

Cibacron Blue F3GA incorporated magnetic poly(2-hydroxyethyl methacrylate) beads for lysozyme adsorption

Lysozyme adsorption onto Cibacron Blue F3GA incorporated magnetic poly(2-hydroxyethyl methacrylate) (mag-PHEMA) beads was investigated. Mag-PHEMA beads (80–120 μm) were produced by dispersion polymerization. The triazine dye Cibacron Blue F3GA was incorporated covalently as a dye ligand. These dye-incorporated beads, having a swelling ratio of 34% (w/w) and carrying different amounts of Cibacron Blue F3GA (12.2–42.8 μmol/g) were used in lysozyme adsorption studies. The effects of the initial concentration, pH, ionic strength, and temperature on the adsorption efficiency of dye-incorporated beads were studied in a batch reactor. The effects of the Cibacron Blue F3GA loading on the lysozyme adsorption were also studied. The nonspecific adsorption of lysozyme on the mag-PHEMA beads was 0.8 mg/g. Cibacron Blue F3GA attachment significantly increased the lysozyme adsorption up to 295 mg/g. More than 90% of the adsorbed lysozyme was desorbed in 1 h in a desorption medium containing 1.0M KSCN at pH 8.0. © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 93: 719–725, 2004

Glucose oxidase and catalase adsorption onto Cibacron Blue F3GA-attached microporous polyamide hollow-fibres

The aim of this study was to explore in detail the performance of polyamide hollow fibers to which Cibacron Blue F3GA was attached for adsorption of proteins. Model proteins were glucose oxidase, as a flavo-enzyme and contains two tightly bound flavine adenine dinucleotide cofactor, and catalase as a heme-containing metallo-enzyme. The hollow fiber structure was characterized by scanning electron microscopy. These dye-carrying hollow-fibers (35.8 μmol/g) were used in the glucose oxidase and catalase adsorption–elution studies. The non-specific adsorption values were 1.25 mg/g for glucose oxidase and 1.97 mg/g for catalase. Cibacron Blue F3GA attachment increased the adsorption capacity up to 248 mg/g. Langmuir adsorption model was found to be applicable in interpreting glucose oxidase and catalase adsorption by Cibacron Blue F3GA-attached hollow fibers. Significant amount of the adsorbed proteins (up to 97%) was eluted in 1 h in the elution medium containing 1.0 M NaSCN at pH 8.0. In order to determine the effects of adsorption and elution conditions on possible conformational changes of studied protein structures, fluorescence spectrophotometry was employed. It was concluded that polyamide dye-affinity hollow-fibers can be applied for glucose oxidase and catalase adsorption without causing any significant conformational changes. Repeated adsorption/elution processes showed that these dye-attached hollow-fibers are suitable for glucose oxidase and catalase separation.

Bilirubin removal from human plasma by dye affinity microporous hollow fibers

Bioaffinity adsorption has a unique and powerful role as a support tool in the removal of toxic substances from human plasma. Synthetic hollow-fiber membranes have advantages as support matrices in comparison to conventional hemoperfusion columns because they are not compressible and they eliminate internal diffusion limitations. In this study, Cibacron Blue F3GA was covalently attached onto commercially available microporous polyamide hollow-fiber membranes for bilirubin removal from hyperbilirubinemic human plasma. Different amounts of Cibacron Blue F3GA were attached on the polyamide hollow-fibers by changing the dye-attachment conditions, i.e., initial dye concentration, addition of sodium carbonate, and sodium chloride. The maximum amount of Cibacron Blue F3GA attachment was obtained at 42.5 μmol g−1 when the hollow fibers were treated with 3 M HCl for 30 min before performing the dye attachment. The nonspecific bilirubin adsorption on the unmodified polyamide hollow-fiber membranes was 0.65 mg g−1 from human plasma. Higher bilirubin adsorption capacities, of up to 39.7 mg g−1, were obtained with the Cibacron Blue F3GA-attached polyamide hollow-fiber membranes. Further increase in bilirubin adsorption was obtained as 48.9 mg g−1. Bilirubin molecules interacted with these adsorbents directly. Contribution of albumin adsorption on the bilirubin adsorption was much pronounced. Bilirubin adsorption increased with increasing temperature and maximum adsorption was observed at 37°C.

Zinc ion-promoted adsorption of lysozyme to Cibacron Blue F3GA-attached microporous polyamide hollow-fiber membranes

Dye-affinity and metal chelate affinity adsorption are increasingly used for protein separation. Synthetic hollow fiber membranes have advantages as support matrices in comparison to conventional bead supports because they are not compressible and they eliminate internal diffusion limitations. The goal of this study was to explore in detail the performance of hollow fibers composed of modified polyamide to which Cibacron Blue F3GA and Zn(II) were attached for adsorption of lysozyme. The polymer matrix was characterized by scanning electron microscopy. These dye-affinity and Zn(II) chelated hollow-fibers were used in the lysozyme adsorption–elution studies. The effects of initial concentration of lysozyme and medium pH on the adsorption efficiency of dye-attached and metal-chelated hollow-fibers were studied in a batch reactor. The effect of Zn(II) loading on lysozyme adsorption was also studied. The non-specific adsorption of lysozyme on the polyamide hollow-fibers was 1.8 mg g −1. Cibacron Blue F3GA attachment significantly increased the lysozyme adsorption up to 63.2 mg g −1. Lysozyme adsorption capacity of the Zn(II) chelated hollow-fibers (144.2 mg g −1) was greater than that of the Cibacron Blue F3GA-attached hollow-fibers. A significant amount of the adsorbed lysozyme (up to 97%) was eluted in 1 h in the elution medium containing 1.0 M NaSCN at pH 8.0 and 25 mM EDTA at pH 4.9. In order to examine the effects of separation conditions on possible conformational changes of lysozyme structure, fluorescence spectrophotometry was employed. We conclude that dye- and metal-chelate affinity chromatography with polyamide hollow-fibers can be applied for lysozyme adsorption without causing any significant conformational changes and denaturation. Repeated adsorption/elution processes showed that these novel dye-attached and Zn(II) chelated hollow-fibers are suitable for lysozyme adsorption.

Poly(vinylalcohol) coated/Cibacron Blue F3GA-attached polypropylene hollow fiber membranes for removal of cadmium ions from aquatic systems

Dye-affinity adsorption is increasingly used for heavy metal removal from aquatic systems. Synthetic hollow fiber membranes have advantages as support matrices in comparison to conventional microbead adsorbents because they are not compressible and they eliminate internal diffusion limitations. The aim of this study was to explore in detail the performance of polyvinyl alcohol coated/Cibacron Blue F3GA-attached polypropylene hollow fibers for removal of Cd(II) ions. The internal matrix was characterized by scanning electron microscopy. Commercially available polypropylene hollow fiber membranes were coated with polyvinyl alcohol (PVA). The PVA adsorption conditions were optimized to increase the amount of PVA by changing the initial concentration of PVA, and adding NaCl. The maximum PVA adsorption capacity was 22.6 mg/g. These hollow fiber membranes carrying Cibacron Blue F3GA (87.9 μmol/g) were used in the Cd(II) removal from aqueous solutions containing different amounts of ions (10–500 mg/l) and at different pH values (2.0–8.0). Cd(II) adsorption capacity increased with time during the first 40 min and then leveled off toward the equilibrium adsorption capacity. Adsorption of Cd(II) ions on the PVA coated polypropylene hollow fiber membranes was 1.2 mg/g. Cibacron Blue F3GA-attachment significantly increased the Cd(II) adsorption (46 mg/g polymer). The adsorption of Cd(II) ions increased with increasing pH and reached a plateau value at around pH 5.0. Competitive heavy metal adsorption from aqueous solutions containing Cd(II), Cu(II), Pb(II) and Hg(II) was also investigated. The adsorption capacities are 1.75 mg/g for Cu(II); 3.46 mg/g for Hg(II); 5.86 mg/g for Pb(II) and 18.45 mg/g for Cd(II) ions. These results may be considered as an indication of higher specificity of the Cibacron Blue F3GA-attached polypropylene hollow fibers for the Cd(II) comparing to other ions. Modified hollow fibers can be regenerated by washing with a solution of nitric acid (0.1 M). The maximum regeneration value was as high as 95%. These modified membranes are suitable for repeated use for more than three adsorption–desorption cycles without considerable loss of Cd(II) adsorption capacity.

Human serum albumin chromatography by Cibacron Blue F3GA-derived microporous polyamide hollow-fiber affinity membranes

An affinity dye ligand, Cibacron Blue F3GA was covalently attached onto commercially available microporous polyamide hollow-fibre membranes for human serum albumin (HSA) adsorption from both aqueous solutions and human plasma. Different amounts of Cibacron Blue F3GA were incorporated on the polyamide hollow-fibres by changing the dye attachment conditions, i.e. initial dye concentration, addition of sodium carbonate and sodium chloride. The maximum amount of Cibacron Blue F3GA attachment was obtained at 42.5 μmol g −1 when the hollow-fibres were treated with 3 M HCl for 30 min before performing the dye attachment. HSA adsorption onto unmodified and Cibacron Blue F3GA-derived polyamide hollow-fibre membranes was investigated batchwise. The non-specific adsorption of HSA was very low (6.0 mg g −1 hollow-fibre). Cibacron Blue F3GA attachment onto the hollow-fibres significantly increased the HSA adsorption (147 mg g −1 hollow-fibre). The maximum HSA adsorption was observed at pH 5.0. Higher HSA adsorption was observed from human plasma (230 mg HSA g −1 hollow-fibre). Desorption of HSA from Cibacron Blue F3GA derived hollow-fibres was obtained using 0.1 M Tris–HCl buffer containing 0.5 M NaSCN or 1.0 M NaCl. High desorption ratios (up to 98% of the adsorbed HSA) were observed. It was possible to reuse Cibacron Blue F3GA derived polyamide hollow-fibre without significant decreases in the adsorption capacities.

Nonporous monosize polymeric sorbents: Dye and metal chelate affinity separation of lysozyme

Lysozyme adsorption onto dye-attached nonporous monosize poly(2-hydroxyethyl-methacrylate-methylmethacrylate) [poly(HEMA-MMA)] microspheres was investigated. Poly(HEMA-MMA) microspheres were prepared by dispersion polymerization. The monochloro-triazine dye, Cibacron Blue F3GA, was immobilized covalently as dye–ligand. These dye-affinity microspheres were used in the lysozyme adsorption–desorption studies. The effect of initial concentration of lysozyme and medium pH on the adsorption efficiency of dye-attached and metal-chelated microspheres were studied in a batch reactor. Effect of Cu(II) chelation on lysozyme adsorption was also studied. The nonspecific adsorption of lysozyme on the poly(HEMA-MMA) microspheres was 3.6 mg/g. Cibacron Blue F3GA attachment significantly increased the lysozyme adsorption up to 247.8 mg/g. Lysozyme adsorption capacity of the Cu(II) incorporated microspheres (318.9 mg/g) was greater than that of the Cibacron Blue F3GA-attached microspheres. Significant amount of the adsorbed lysozyme (up to 97%) was desorbed in 1 h in the desorption medium containing 1.0M NaSCN at pH 8.0 and 25 mM EDTA at pH 4.9. In order to examine the effects of separation conditions on possible conformational changes of lysozyme structure, fluorescence spectrophotometry was employed. We conclude that dye- and metal-chelate affinity chromatography with poly(HEMA-MMA) microspheres can be applied for lysozyme separation without causing any significant changes and denaturation. Repeated adsorption/desorption processes showed that these novel dye-attached monosize microspheres are suitable for lysozyme adsorption. © 2000 John Wiley & Sons, Inc. J Appl Polym Sci 76: 115–124, 2000

Adsorption of heavy metal ions onto ethylene diamine-derived and Cibacron Blue F3GA-incorporated microporous poly(2-hydroxyethyl methacrylate) membranes

Microporous poly(2-hydroxyethylmethacrylate) (PHEMA) membranes carrying ethylene diamine (EDA) and Cibacron Blue F3GA were prepared for the removal of heavy metal ions (i.e. mercury, copper, lead and cadmium) from aqueous solutions containing different amounts of these ions (5–700 μg l −1) and at different pH values (2.0–8.0). The non-specific adsorption of heavy metal ions on the underived membranes was very low (3.3 mmol/m 2 for Hg(II), 0.5 mmol/m 2 for Cu(II), 1.2 mmol/m 2 for Pb(II) and 1.1 mmol/m 2 for Cd(II)). Cibacron Blue F3GA attachment significantly increased the heavy metal adsorption (16.3 mmol/m 2 for Hg(II), 19.9 mmol/m 2 for Cu(II), 23.4 mmol/m 2 for Pb(II) and 38.4 mmol/m 2 for Cd(II)). When the heavy metal ions competed (in the case of the adsorption from a mixture) the adsorption capacities were 7.1 mmol/m 2 for Hg(II), 17.9 mmol/m 2 for Cu(II), 6.2 mmol/m 2 for Pb(II) and 9.1 mmol/m 2 for Cd(II). The observed order in adsorption was found to be Cd(II)>Pb(II)>Cu(II)>Hg(II) for non-competitive conditions. The adsorption of heavy metal ions increased with increasing pH and reached a plateau value at around pH 5.0. Desorption of heavy metal ions was achieved using 0.1 M HNO 3. These membranes are suitable for repeated use for more than five cycles without considerable loss of adsorption capacity.

Cibacron Blue F3GA and Cu(II) derived poly(2-hydroxyethylmethacrylate) membranes for lysozyme adsorption

Lysozyme adsorption onto Cibacron Blue F3GA and Cu(II) derived poly(2-hydroxyethyl methacrylate) [poly(HEMA)] membranes was investigated. Microporous poly(HEMA) membranes were prepared by photopolymerization of HEMA. The triazine dye, Cibacron Blue F3GA was attached covalently as a dye-ligand. These dye-membranes with a swelling ratio of 58% (w/w), and carrying different amounts of Cibacron Blue F3GA (between 0.35 and 1.07 μmol cm −2) were used in the lysozyme adsorption studies. The effect of initial concentration and pH on the adsorption efficiency of dye-derived and metal-chelated membranes were studied in a batch reactor. The effect of Cu(II) incorporation on lysozyme adsorption was also studied. The non-specific adsorption of lysozyme on the poly(HEMA) membranes was 0.9 μg cm −2. Cibacron Blue F3GA attachment significantly increased the lysozyme adsorption up to 133.3 μg cm −2. Lysozyme adsorption capacity of the Cu(II) incorporated membranes (165.1 μg cm −2) was greater than that of the Cibacron Blue F3GA-attached membranes. More than 85% of the adsorbed lysozyme was desorbed in 1 h in the desorption medium containing 0.5 M potassium thiocyanate (KSCN) at pH 8.0.

New metal chelate sorbent for albumin adsorption: Cibacron Blue F3GA‐Zn(II) attached microporous poly(HEMA) membranes

Poly(2-hydroxyethyl methacrylate) [poly(HEMA)] membranes were prepared by UV-initiated photopolymerization of HEMA in the presence of an initiator (α-α′-azobis-isobutyronitrile, AIBN). The triazine dye Cibacron Blue F3GA was attached as an affinity ligand to poly(HEMA) membranes, covalently. These affinity membranes with a swelling ratio of 58% and containing 10.7 mmol Cibacron Blue F3GA/m2 were used in the albumin adsorption studies. After dye-attachment, Zn(II) ions were chelated within the membranes via attached-dye molecules. Different amounts of Zn(II) ions [650–1440 mg Zn(II)/m2] were loaded on the membranes by changing the initial concentration of Zn(II) ions and pH. Bovine serum albumin (BSA) adsorption on these membranes from aqueous solutions containing different amounts of BSA at different pH was investigated in batch reactors. The nonspecific adsorption of BSA on the poly(HEMA) membranes was negligible. Cibacron Blue F3GA attachment significantly increased the BSA adsorption up to 92.1 mg BSA/m2. Adsorption capacity was further increased when Zn(II) ions were attached (up to 144.8 mg BSA m2). More than 90% of the adsorbed BSA was desorbed in 1 h in the desorption medium containing 0.5M NaSCN at pH 8.0 and 0.025M EDTA at pH 4.9. © 1998 John Wiley & Sons, Inc. J Appl Polym Sci 68: 657–664, 1998

New metal chelate sorbent for albumin adsorption: Cibacron Blue F3GA-Zn(II) attached microporous poly(HEMA) membranes

Poly(2-hydroxyethyl methacrylate) [poly(HEMA)] membranes were prepared by UV-initiated photopolymerization of HEMA in the presence of an initiator (α-α′-azobis-isobutyronitrile, AIBN). The triazine dye Cibacron Blue F3GA was attached as an affinity ligand to poly(HEMA) membranes, covalently. These affinity membranes with a swelling ratio of 58% and containing 10.7 mmol Cibacron Blue F3GA/m2 were used in the albumin adsorption studies. After dye-attachment, Zn(II) ions were chelated within the membranes via attached-dye molecules. Different amounts of Zn(II) ions [650–1440 mg Zn(II)/m2] were loaded on the membranes by changing the initial concentration of Zn(II) ions and pH. Bovine serum albumin (BSA) adsorption on these membranes from aqueous solutions containing different amounts of BSA at different pH was investigated in batch reactors. The nonspecific adsorption of BSA on the poly(HEMA) membranes was negligible. Cibacron Blue F3GA attachment significantly increased the BSA adsorption up to 92.1 mg BSA/m2. Adsorption capacity was further increased when Zn(II) ions were attached (up to 144.8 mg BSA m2). More than 90% of the adsorbed BSA was desorbed in 1 h in the desorption medium containing 0.5M NaSCN at pH 8.0 and 0.025M EDTA at pH 4.9. © 1998 John Wiley & Sons, Inc. J Appl Polym Sci 68: 657–664, 1998

Cibacron Blue F3GA and Zn(II) Containing Poly(Ethylene Glycol Dimethacrylate-Hydroxyethyl Methacrylate) Microbeads for Albumin Adsorption

Swellable poly (ethylene glycol dimethacrylate-hydroxyethyl methacryate) [poly(EGDMA-HEMA] microbeads, in the size range of 150–200 μm, were produced by a modified suspension copolymerization of EGDMA and HEMA. Cibacron Blue F3GA was attached, then Zn(II) ions were incorporated within the microbeads by chelating with the Cibacron Blue F3GA molecules. The maximum amount of Cibacron Blue F3GA attachment was 16.5 μmol/g polymer. Different amounts of Zn(II) ions (0.4–32.4 mg/g polymer were incorporated on the microbeads by changing the initial concentration of Zn(II) ions and medium pH. Bovine serum albumin (BSA) adsorption onto unmodified, Cibacron Blue F3GA-attached and Cibacron Blue F3GA-Zn(II)-derivatized microbeads was investigated. The non-specific adsorption of BSA was very low (0.051 mg/g polymer). Cibacron Blue F3GA attachment significantly increased the BSA adsorption (60.5 mg/g polymer). The maximum BSA adsorption (122 mg/g polymer) was observed at pH 6.0 when Cibacron Blue F3GA-Zn(II)-derivatized microbeads were used. Desorption of BSA was achieved by using 25 mM EDTA (pH 4.9). High desorption ratios (up to 95% of the adsorbed BSA) were observed. It was possible to reuse Cibacron Blue F3GA-Zn(II)-derivatized microbeads without significant losses in the adsorption capacities.