Chronic Toxicity Tests Research Articles

Comparison of hydrogen peroxide-based advanced oxidation processes for the treatment of azole-containing industrial wastewater

In this study the effectiveness of three types of advanced oxidation processes (AOPs), namely the Fenton, UV/Fenton and UV/H2O2 processes, were compared for the treatment of a lab-prepared wastewater containing five-membered heterocyclic pyrazole, 2-(2-aminoethoxy) ethanol (also known as diglycolamine or DGA), hydrogen peroxide, and inorganic fluoride which are common components of semiconductor wastewater. The Fenton, UV/Fenton (at acidic and near neutral pH) and UV/H2O2 processes with same iron (or no iron) and H2O2 dosing were compared and the results show the three AOPs can remove pyrazole and DGA from the synthetic wastewater rapidly with a simultaneous reduction of total organic carbon (TOC). The UV/Fenton process at initial pH of 8.3 outperformed the other AOPs in terms of overall removal rate due to the synergistic effects of pH-dependent speciation of pyrazole and DGA, direct photolysis of hydrogen peroxide, iron-peroxide complexation and iron-oxalate complexation. The UV/Fenton process at initial pH = 3.0 had the slowest kinetics and the UV/H2O2 process (at pH = 8.3) had the lowest TOC removal efficiency. The successive oxidation of both pyrazole and DGA resulted in the production of formic acid, acetic acid, oxalic acid, nitrate ions and ammonium ions. Actual semiconductor industrial wastewater containing the same principal components as the lab-prepared water along with co-contaminants was treated with the three AOPs and the presence of co-contaminants lowered the removal rate constants of pyrazole and DGA for the Fenton and UV/H2O2 processes but increased those in the UV/Fenton process. Acute and chronic toxicity tests employing ceriodaphnia dubia indicated the wastewater after AOPs treatment showed no toxicity.

Estimation of hazardous concentration of toluene in the terrestrial ecosystem through the species sensitivity distribution approach

Toluene is a highly flammable and commonly used industrial chemical with severe health consequences on humans upon exposure and ingestion. In this study, multispecies bioassays were conducted using a species sensitivity distribution approach to determine acute and chronic hazardous concentrations of toluene in soil. Acute and chronic toluene toxicity tests were conducted with seven soil species from four taxonomic groups. The results from the toxicity tests were used to estimate the acute and chronic HC5 (hazardous concentration for 5 % of species) of toluene in the terrestrial environment at 58.9 (5.4–639.6) mg kg−1 and 2.2 (0.2–19.8) mg kg−1, respectively. To the best of our knowledge, this is the first study to estimate the hazardous concentration of toluene in soil by conducting a battery of bioassays. These values can be used as references for the environmental risk assessment of chemical accidents involving toluene and estimating its impact on soil to protect the terrestrial environment.

Species sensitivity distributions for ethylparaben to derive protective concentrations for soil ecosystems.

Ethylparaben is used as an antifungal preservative. Although some countries have implemented regulations for human exposure to parabens, environmental regulations for ethylparaben have not been established. This study provides new toxicological data for ethylparaben, for which data regarding soil organisms were previously lacking. Although ethylparaben toxicity has been reported in other species, we present herein the first comprehensive study of its toxicity in soil organisms. We used 12 test species (Lycopersicon esculentum, Vigna radiata, Hordeum vulgare, Oryza sativa, Eisenia andrei, Folsomia candida, Lobella sokamensis, Caenorhabiditis elegans, Chlamydomonas reinhardtii, Chlorococcum infusionum, Chlorella sorokiniana, Chlorella vulgaris) from eight taxonomic groups for acute bioassays and nine test species (L. esculentum, V. radiata, H. vulgare, O. sativa, C. reinhardtii, C. infusionum, C. sorokiniana, and C. vulgaris) from five taxonomic groups for chronic bioassays. A suite of acute and chronic toxicity tests, using 21 soil species, was conducted to estimate EC50 values, which facilitated the construction of species sensitivity distributions (SSDs) and the calculation of protective concentrations (PCs). Acute and chronic PC95 values (protective concentration for 95% of species) for ethylparaben were estimated to be 14 and 5mg/kg dry soil, respectively. To the best of our knowledge, this is the first study to evaluate the toxicity of ethylparaben to soil species and derive PCs for soil ecosystems based on SSDs. Therefore, the data presented in this study can be used as a basis for further investigations of paraben toxicity to the soil environment.

Development of an Alternative Test System for Chronic Testing of Lotic Macroinvertebrate Species: A Case Study with the Insecticide Imidacloprid.

There are currently few suitable test systems for the chronic toxicity testing of aquatic macroinvertebrates under stream conditions. Therefore, a new test system mimicking running water conditions was developed for testing with lotic insects. This system uses small test cages, with 10 of these suspended inside each 25-L container and rotating at 0.1 m/s, to create a water flow for the individual organism inside each cage. To test the performance of the new exposure system, chronic effects (21 d) of the neonicotinoid imidacloprid were investigated with field-collected larvae of the stonefly Protonemura sp. Endpoints were survival, growth, and/or emergence (depending on the developmental stage of the larvae at the start of the exposure). Two experiments conducted 1 yr apart showed good reproducibility: growth 10% effect concentration (EC10) values were 15.3 and 18.5 μg/L and no-observed-effect concentration (NOEC) values were 30.3 and 21.5 μg/L. A third experiment, performed with further-developed larval instars, showed a significant effect of imidacloprid on emergence (with EC10 of 5.97 μg/L and NOEC of 2.89 μg/L) and a significant effect on survival (with median lethal concentration of 44.7 µg/L). The results of the present study show that the newly developed test system provides a suitable approach for toxicity testing with stonefly larvae and potentially for other lotic macroinvertebrate species. Environ Toxicol Chem 2021;40:2229-2239. © 2021 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

Acute and Chronic Toxicity Testing of Drinking Water Treatment Residuals in Freshwater Systems.

The beneficial use of drinking water treatment residuals (DWTRs) faces barriers due primarily to uncertainties and concerns about their potential environmental impacts. We used total and water leachable toxic metal concentrations and 2 benthic organism-based bioassays to identify suitable DWTR substrates for introduction to freshwater systems. Using total metal contents and the consensus probable effect concentration concept, 3 DWTRs were selected and used in elutriate and toxicity studies. The concentrations of water leachable Ag, As, Cd, Cu, Cr, Ni, Pb, and Zn were below the US Environmental Protection Agency's ambient water quality criteria. Using the long-term 65-d life cycle Chironomus tentans test and 4 different endpoints (survival, adult emergence, egg case production, and number of eggs produced per female), no statistical differences were found between the DWTR treatments and the controls. Similarly, results obtained using the 10-d Hyalella azteca test showed no toxicity. However, although both survival and growth were recorded in all bioassays, the results of the 10-d C. tentans and the 28-d H. azteca tests were ambiguous. For C. tentans, 2 of the 3 DWTRs resulted in significantly lower survival rates compared to the controls. For H. azteca, no significant growth differences were observed between controls and DWTR treatments, but 2 of the 3 DWTRs resulted in significantly lower survival rates than the controls. Overall, these results suggest that certain DWTR substrates could be suitable for introduction to aquatic systems. Environ Toxicol Chem 2021;40:2005-2014. © 2021 SETAC.

Acute and Sub-chronic Toxicity Study of Recombinant Bovine Interferon Alpha in Rodents.

IntroductionRecombinant bovine interferon alpha (rBoIFN-α) has been demonstrated to have antiviral activity. However, no conduct of acute or chronic toxicity tests has been reported.Material and MethodsSpecific pathogen-free Sprague Dawley rats were administered doses at different concentrations through intraperitoneal or intravenous injection. After the administration (single for an acute toxicity test over 14 days or daily for a sub-chronic toxicity test over 30 days), the rats’ behaviour and other indicators and the degree of toxic reaction were continuously monitored. Blood was collected for haematological and serum biochemical examinations. At the end of the experiments, the rats were sacrificed for necropsy and histopathological tissue analysis.ResultsThe external performance, behaviour characteristics, and changes in body temperature and body weight of the rats in each subgroup were comparable to the normal control subgroup. Except for a few cases, there were no lesions in the viscera’s pathological structures, and the blood parameters and biochemical indicators were not noticeably different from those of the control subgroup.ConclusionThis study suggests that rBoIFN-α seems to be safe for rats, and its use may foster the development of the cattle industry in China by protecting livestock health.

Toxicity of Mining-Contaminated Lake Sediments to Lumbriculus variegatus

Boreal lakes with soft water and low buffering capacity are susceptible to excess ion loading resulting from metal mining. The impact of two Finish mining sites in downstream lakes was assessed with a chronic sediment toxicity test using a laboratory-reared freshwater Lumbriculus variegatus (Oligochaeta). The test organisms were exposed to mining-contaminated natural lake sediments and hypolimnion water (HLW) or artificial freshwater (AFW) as overlying water in two independent experimental setups. In both test setups, growth and reproduction of L. variegatus were lower in sediments from the lakes receiving high amount of mining effluents from the mines nearby. In the biomining site, the main contaminants in the recipient lakes were the ore metals Ni and Zn, while in the lakes affected by the conventional underground mine, they were Cu and Zn. These metals accumulated in L. variegatus especially in the setup with natural HLW above the sediment. Growth and reproduction were lower in the HLW than in the AFW setup. The mining-contaminated sediments did not support optimum growth or reproduction of L. variegatus in comparison to the local reference sediments. Decline of pH in the unbuffered natural sediments brought up challenges in the assessment of metal-contaminated lake sediments with high sulfur content, and a need to develop new tools for their risk assessment.

The effects of pulse exposures of metal toxicants on different life stages of the tropical copepod Acartia sinjiensis

Effluent discharges can potentially result in high concentrations of metals entering aquatic environments for short durations, ranging from a few hours to days. The environmental risks of such exposures are challenging to accurately assess. Risk assessment tools for effluent discharges include comparison of toxicant concentrations with guideline values and the use of direct toxicity assessments, both of which were designed to assess continuous, rather than pulse, contaminant exposures. In this study, a chronic pulse-exposure toxicity test was developed using the tropical euryhaline calanoid copepod Acartia sinjiensis. This copepod has a rapid life cycle and is highly sensitive to metal contaminants, with 50% effect concentrations (chronic EC50) for larval development of 1.7, 8.6 and 0.7 μg L−1 for copper, nickel and zinc, respectively. The toxicities of copper and nickel were assessed as a continuous exposure (78 h) and as pulses (3, 6 and 18 h) initiated at varying life stages, from egg to copepodite, and measured larval development over 78 h. Generally, 24-h old nauplii were more sensitive or of similar sensitivity to copper and nickel pulses than 48-h old nauplii. The 78-h test duration enabled observations of chronic effects following pulse exposures, which frequently occurred in the absence of acute effects. The EC50 values for pulse exposures were higher than those of continuous exposure by up to approximately 16-fold and 15-fold for copper and nickel, respectively. When metal-pulse exposure concentrations were expressed using the time-weighted averaged concentration (TAC), resultant concentration response curves were similar to those in continuous exposures to the same metal, suggesting that thresholds based on continuous exposures were also protective for pulse exposures to these metals. This research improves our understanding of the toxicity of pulse contaminant exposures and assists with developing improved approaches to for the risk assessment and regulation of short-term contaminant discharges.

Effects of Different Ammonia Levels on Tribenuron Methyl Toxicity in Daphnia magna.

The present study investigates the toxicity of the herbicide tribenuron methyl (TBM) as an anthropogenic agent and ammonia as an abiotic factor on Daphnia magna at environmentally relevant concentrations. These stressors may coexist in surface waters in agricultural regions. To achieve this objective, D. magna were exposed to TBM at a nominal concentration of 0.81μg/L in association with a low ammonia (LA) concentration of 0.65mg/L and a high ammonia (HA) concentration of 1.61mg/L in acute toxicity tests of 96-h duration and chronic toxicity tests of 21-day duration. The D. magna also were exposed to TBM, HA, and LA singly. The D. magna were analysed for various biomarkers of sublethal toxicity. Glutathione peroxidase (GPx), glutathione S-transferase (GST), cholinesterase (ChE) enzyme activities, and levels of thiobarbituric acid reactive substances (TBARS) and total protein were determined spectrophotometrically. Mitochondrial membrane potential (MMP) was analysed by microscopy with fluorescence staining. Cytochrome c and 5' AMP-activated protein kinase (AMPK) were analysed by Western blotting. Morphometric properties were examined microscopically. This is the first study in which AMPK, an indicator of intracellular energy, was measured in D. magna. GST and ChE enzyme activities and TBARS and total protein levels did not change during acute exposures (i.e., 96h) in all treatments. GPx activity increased in D. magna from the HA + TBM treatment compared with single-exposure groups. The level of cytochrome c protein was elevated in D. magna from the LA and LA + TBM treatments. AMPK protein levels increased in all treatments with daphnids, except in the LA group. MMP was depolarised in D. magna from all treatments, whereas the most notable change was observed in HA + TBM mixture group in chronic exposures. The results show that GST and ChE may not be sensitive biomarkers for evaluating the sublethal toxic effects to D. magna exposed to environmentally relevant concentrations of ammonia and TBM. Acute and chronic exposure to ammonia and TBM probably caused an energetic crisis in D. magna. Therefore, AMPK and MMP are promising biomarkers for these toxicants.

Development of a Sublethal Chronic Toxicity Test for the Northern Trout Gudgeon, Mogurnda mogurnda, and Application to Uranium, Magnesium, and Manganese.

Many international guidance documents for deriving water quality guideline values recommend the use of chronic toxicity data. For the tropical fish northern trout gudgeon, Mogurnda mogurnda, 96-h acute and 28-d chronic toxicity tests have been developed, but both tests have drawbacks. The 96-h toxicity test is acute and has a lethal endpoint; hence it is not a preferred method for guideline value derivation. The 28-d method has a sublethal (growth) endpoint, but is highly resource intensive and is high risk in terms of not meeting quality control criteria. The present study aimed to determine the feasibility of a 7-d larval growth toxicity test as an alternative to the 96-h survival and 28-d growth tests. Once the method was successfully developed, derived toxicity estimates for uranium, magnesium, and manganese were compared with those for other endpoints and tests lengths within the literature. As a final validation of the 7-d method, the sensitivity of the 7-d growth endpoint was compared with those of 14-, 21-, and 28-d exposures. Fish growth rate, based on length, over 7 d was significantly more sensitive compared with existing acute toxicity endpoints for magnesium and manganese, and was similarly sensitive to existing chronic toxicity endpoints for uranium. For uranium, the sensitivity of the growth endpoint over the 4 exposure periods was similar, suggesting that 7 d as an exposure duration is sufficient to provide an indication of longer term chronic growth effects. The sensitivity of the 7-d method, across the 3 metals tested, highlights the benefit of utilizing the highly reliable short-term 7-d chronic toxicity test method in future toxicity testing using M. mogurnda. Environ Toxicol Chem 2021;40:1596-1605. © 2021 Commonwealth of Australia. Environmental Toxicology and Chemistry © 2021 SETAC.

Chronic Toxicity of Cobalt to Marine Organisms: Application of a Species Sensitivity Distribution Approach to Develop International Water Quality Standards.

Water quality standards for cobalt (Co) have yet to be developed for the European Union or the United States. The primary objective of the present study was to produce a data set comprising marine Co toxicity data that could be used by both the European Union and the United States to determine a predicted-no-effect concentration and ambient water quality criteria, respectively. Ten marine species, ranging from algae to fish, were subjected to chronic Co toxicity tests that were designed to meet international water quality testing standards. Chronic 10% effect concentration values ranged from a low of 1.23 µg dissolved Co/L for red algae (Champia parvula) to a high of 31 800 µg dissolved Co/L for sheepshead minnow (Cyprinodon variegatus). The species sensitivity ranking for chronic marine Co exposure was as follows (from most to least sensitive): C. parvula > Neanthes arenaceodentata (polychaete) > Americamysis bahia (mysid shrimp) > Skeletonema costatum (marine diatom) > Dendraster excentricus (sand dollar) > Mytilus galloprovincialis (mussel) > Strongylocentrotus purpuratus (purple sea urchin) > Crassostrea gigas (oyster) > Dunaliella tertiolecta (marine flagellate) > C. variegatus. Chronic test results indicated that invertebrate and plant species were substantially more sensitive to Co exposure than fish. The chronic toxicity data were used to calculate a species sensitivity distribution, from which a hazardous concentration 5th percentile of 7.09 µg dissolved Co/L (95% CI 0.025-47.3 µg Co/L) was derived. Environ Toxicol Chem 2021;40:1405-1418. © 2021 SETAC.

Toxicity of fipronil and 2,4-D formulations (alone and in a mixture) to the tropical amphipod Hyalella meinerti.

Conventional farming uses a large volume of pesticides that may reach aquatic ecosystems. This is also the case for the insecticide fipronil and the herbicide 2,4-D, which are widely used in many crops. This study aimed at evaluating the individual and mixture toxicity of these pesticides to the tropical amphipod Hyalella meinerti. To this end, acute toxicity tests (96 h) were conducted. Chronic bioassays (10 days) were also carried out, in which the body length and dry biomass were evaluated as endpoints. In addition, a complete factorial mixture chronic toxicity test was carried out. H. meinerti was sensitive to fipronil in the acute toxicity tests, with a LC50-96-h of 0.86 μg L-1 (95% CI 0.26-0.46), and no acute effects were observed after 2,4-D exposure even at the highest test concentration of 100 mg L-1. In the chronic toxicity tests, all tested concentrations of both pesticides decreased the growth of H. meinerti, in which losses on biomass reached 45% and 65% for 2,4-D and fipronil, respectively. The pesticide mixture indicated antagonism although it still significantly decreased the body growth. The results obtained indicate a high sensitivity of H. meinerti exposed to environmentally realistic concentrations, demonstrating that there are risks for the species in real field conditions.

Liver Histopathological Measurement Due to Maximum Dosage for Acute Oral Toxicity Test Using Ethanol Extract Of Coffee Pulp In Female Mice

Coffee processing into fast food ingredients that must be done in order to get maximum results. Behind the processing there are various problems that arise, one of them are the presence of solid waste in the form of pulp coffee. Based on several studies, coffee pulp turned out to have high antioxidants.This research was conducted an acute oral toxicity test of the maximum dose (2000 mg/kg BW) of ethanol extract of coffee pulp was tested on mice for 24 hours. Acute oral toxicity studies of coffee pulp based on guidelines for National Drug and Food Control in female mice. The study was conducted in 2 groups of test animals consisting of a negative control group and an acute oral treatment group. Each group of 5 mice were treated for 24 hours. After treatment, the object was analyzed by histological observations descriptively. Descriptive histological analysis (qualitative analysis) of the liver of female mice showed no difference between the study groups. The treatment group that was given ethanol extract of coffee pulp showed a normal nucleus which was shown by the appearance of scattered chromatin beads. This is in line with the description of the normal cytoplasm like a group of normal mice. The maximum dose of 2,000 mg / kg for acute oral testing for 24 hours does not show microscopic changes in female mice.Recommendation for further research is to conduct oral subchronic and chronic toxicity testing in the normal group and in groups given free radical-producing compounds with various examinations of other organs.
 
 Keywords: health drinks, antioxidant, acute toxicity, maximum dose.

Acute Polychlorinated Biphenyl Benthic Invertebrate Toxicity Testing to Support the 2017 Chronic Dose-Response Sediment Injury Model.

As managers and decision makers evaluate pollutant risk, it is critical that we are able to measure an assessment of the injury. Often, these estimates are difficult to determine for benthic organisms, so in 2017 a chronic polychlorinated biphenyl (PCB) sediment dose-response model to predict benthic invertebrate injury was proposed. Given both natural resource trustee and consultant questions following publication concerning that the aqueous chronic toxicity testing data used in the 2017 model development were primarily from the 1970s and 1980s, this follow-up short communication is meant to provide the user some additional data that are more recent. With the advances in analytical and quantitative environmental chemistry (i.e., better detection limits and congener separation), we chose to complete acute aquatic toxicity testing using 3 estuarine invertebrates and lethal endpoints (20 and 50% lethal concentrations). This acute testing was selected because chronic aquatic testing for PCBs outside of the data used in the 2017 study was not available to us. The aquatic results used in the present study were changed to sediment using equilibrium partitioning, as done in the 2017 chronic model, after using the same organic-carbon partition coefficient and total organic carbon for our equilibrium partitioning (EqP)-measured calculations. Based on these acute aquatic toxicity results and a general acute-to-chronic injury concentration ratio of approximately 10, we found that the 2017 model was valid and, hence, that a 1.0 µg/g chronic PCB sediment criterion is a reasonable estimation of potential benthic invertebrate injury. This was followed by spiked sediment tests where percent acute sediment injury was compared to the EqP-derived chronic value and the results from 2017; modest agreement is shown. Environ Toxicol Chem 2021;40:1188-1193. Published 2020. This article is a U.S. Government work and is in the public domain in the USA.

Quantifying the consequence of applying conservative assumptions in the assessment\xa0of oil spill effects on polar cod (Boreogadus saida) populations

In order to assess the potential impact from oil spills and decide the optimal response actions, prediction of population level effects of key resources is crucial. These assessments are usually based on acute toxicity data combined with precautionary assumptions because chronic data are often lacking. To better understand the consequences of applying precautionary approaches, two approaches for assessing population level effects on the Arctic keystone species polar cod (Boreogadus saida) were compared: a precautionary approach, where all exposed individuals die when exposed above a defined threshold concentration, and a refined (full-dose-response) approach. A matrix model was used to assess the population recovery duration of scenarios with various but constant exposure concentrations, durations and temperatures. The difference between the two approaches was largest for exposures with relatively low concentrations and short durations. Here, the recovery duration for the refined approach was less than eight times that found for the precautionary approach. Quantifying these differences helps to understand the consequences of precautionary assumptions applied to environmental risk assessment used in oil spill response decision making and it can feed into the discussion about the need for more chronic toxicity testing. An elasticity analysis of our model identified embryo and larval survival as crucial processes in the life cycle of polar cod and the impact assessment of oil spills on its population.

Seasonal Response of Daphnia pulex to Cyanobacterial Extracts at Different Temperatures in Valle de Bravo Reservoir (Mexico)

Valle de Bravo reservoir supplies drinking water to 40% of Mexico City. Here we present data on the population growth and life-table demography of the cladoceran Daphnia pulex, cultured at temperatures of 20 °C and 25 °C and with different concentrations of the crude extracts from blooms of Microcystis aeruginosa, collected in January, and Woronichinia naegeliana, collected in September. We hypothesized that Daphnia pulex would be more sensitive at the higher temperature and to toxins from W. naegeliana as these blooms have been shown to be more toxic to rotifers. We extracted the toxins and conducted acute toxicity tests at eight concentrations of microcystins at 20 °C. The LC50 was 26.8 µg/L and 11.5 µg/L, respectively, for Microcystis and Woronichinia samples. The chronic toxicity tests included population growth and life-table demography studies at 5 and 10% of the LC50 concentration, at 20 °C and 25 °C. Four replicates for each of the three treatments, which consisted of treatments with low and high cyanotoxin levels and a control without cyanotoxins, were set up. The population growth rate ranged from 0.18 to 0.42 d−1 on the extracts from M. aeruginosa (January) and from 0.2 to 0.31 on extracts from W. naegeliana. Daphnia, being better adapted to cooler temperatures, was more adversely affected at 25 °C than 20 °C. The adverse effect of cyanobacterial extracts was greater from Microcystis than Woronichinia blooms. The tolerance of Daphnia pulex to cyanotoxins depends on the bloom-forming species and the temperature.

Assessment of the impacts of microbial plant protection products containing Bacillus thuringiensis on the survival of adults and larvae of the honeybee (Apis mellifera)

This study was aimed at evaluating the effect of a microbial pest-controlling product (MPCP) with the active substance Bacillus thuringiensis ssp. aizawai (strain: ABTS-1857) on adults and larvae of honeybees. To determine the contamination levels of Bt spores in different matrices, a colony-feeding study under semi-field conditions was performed. Furthermore, two chronic adult trials and a chronic larval study were conducted under laboratory conditions to test the effects of different concentrations of the plant protection product (PPP) on the development and mortality. Possible modifications of the chronic oral toxicity test were assessed by additional pollen feeding. Our results showed that Bt spores were detected in all matrices over the entire test duration in different concentrations, decreasing over time. The survival of adult bees and larvae was negatively affected in laboratory conditions after a chronic exposure to the MPCP depending on the tested concentrations. Moreover, the earliest sign of bee mortality, resulting from exposure to ABTS-1857, was recorded only after 96 h at the highest tested concentration. Pollen feeding to adults significantly increased the survival of the treated bees. In conclusion, the PPP with the Bt strain ABTS-1857 showed an effect on the mortality of adults and larvae under laboratory conditions. Further studies with Bt-based PPPs under realistic field conditions are necessary to evaluate the potential risk of those MPCPs on honeybees.

Multigenerational Toxic Effects on Daphnia magna Induced by Silver Nanoparticles and Glyphosate Mixture.

Multigenerational toxicological effects of a binary mixture of silver nanoparticles (AgNPs) with glyphosate were identified in Daphnia magna using acute and chronic toxicity tests. Acute toxicity interactions were analyzed with the Abbott method. In the chronic tests, the survival, growth, reproduction, and age at first brood were evaluated for the parents and the exposed (F1E) and non-exposed (F1NE) descendants. The scales tested for binary mixture, at the acute level, presented antagonistic and additive interactions, possibly associated with the complexation of the AgNPs by glyphosate. Multigenerational chronic effects related to the parameters, reproduction, and age at first brood were observed in the descendants tested with the individual compounds, with no recovery for F1E and F1NE. In organisms exposed to binary mixture, there was a delay in the age at first brood and also a significant change in the reproduction parameter, with a strong reduction for the parents, F1E, and F1NE, indicating a higher toxicity than the compounds tested individually. Although the results for acute interactions between AgNP and glyphosate did not provide clear evidence, multigenerational chronic binary mixture trials have resulted in unexpected toxicity compared with individual treatments, increasing the concerns associated with this co-exposure in other scenarios. Therefore, the interaction of binary mixture with the organisms merits further investigation and the results reported in the present study will be useful in this regard. Environ Toxicol Chem 2021;40:1123-1131. © 2020 SETAC.

Threshold concentrations of the road salt for adverse effects on females and resting eggs of cladoceran Moina macrocopa

The salinization of freshwaters due to the use of deicing road salts is a serious anthropogenic threat. We investigated the effects of the road deicer, which is mostly composed (ca. 70%) of NaCl, on the life cycle parameters of the cladoceran Moina macrocopa in acute and chronic toxicity tests and on the hatching success of resting eggs exposed to sediments contaminated with the road salt. The negative effects of the road salt on survival and life cycle parameters of animals were observed at concentrations above 5 g L−1. The 6-month exposure of resting eggs to contaminated sediments had a consistent but relatively weak effect on the postexposure hatching of resting eggs. Experiments demonstrated that the concentration of the deicer in the top water layer in the water-sediment systems is more important for the hatching success of resting eggs than the salt content in the sediment. Only 2.2 ± 1.9% of resting eggs hatched when the deicer content in the top water layer was equal to 12 g L−1. Lethal effects on hatchlings were observed starting from the deicer content in the water equal to 1 g L−1, and 97.0 ± 0.8% of hatchlings were dead at the deicer content in the water equal to 5 g L−1. Thus, the resilience of resting eggs to the contaminated sediments can ensure the replenishment of the population from the egg bank after the salinity disturbance is diminished but the negative effects of the elevated salt content in surface waters on active population will be manifested at lower salinities.

Development and Validation of a Mixture Toxicity Implementation in the Dynamic Energy Budget-Individual-Based Model: Effects of Copper and Zinc on Daphnia magna Populations.

Mechanistic population models are gaining considerable interest in ecological risk assessment. The dynamic energy budget approach for toxicity (DEBtox) and the general unified threshold model for survival (GUTS) are well-established theoretical frameworks that describe sublethal and lethal effects of a chemical stressor, respectively. However, there have been limited applications of these models for mixtures of chemicals, especially to predict long-term effects on populations. We used DEBtox and GUTS in an individual-based model (IBM) framework to predict both single and combined effects of copper and zinc on Daphnia magna populations. The model was calibrated based on standard chronic toxicity test results with the single substances. A mixture toxicity implementation based on the general independent action model for mixtures was developed and validated with data from a population experiment with copper and zinc mixtures. Population-level effects of exposure to individual metals were accurately predicted by DEB-IBM. The DEB-IBM framework also allowed us to identify the potential mechanisms underlying these observations. Under independent action the DEB-IBM was able to predict the population dynamics observed in populations exposed to the single metals and their mixtures (R2 > 65% in all treatments). Our modeling shows that it is possible to extrapolate from single-substance effects at the individual level to mixture toxicity effects at the population level, without the need for mixture toxicity data at the individual level from standard mixture toxicity tests. The application of such modeling techniques can increase the ecological realism in risk assessment. Environ Toxicol Chem 2021;40:513-527. © 2020 SETAC.