Chronic Gastritis Patients Research Articles

MEDICO-SOCIAL VALUE OF OSTEOARTHRITIS. SECONDARY PREVENTION AND TREATMENT OF OSTEOARTHRITIS IN COMORBIDITY WITH CHRONIC GASTRITIS

Introduction: Medico-social significance of osteoarthritis is due to a number of factors, one of which is associated with the need for long-term anti-inflammatory therapy, which is associated with undesirable side effects. The aim: Identify the features of the course of chronic gastritis in patients taking selective NSAIDs for osteoarthritis. Materials and methods: Were examined 122 patients with osteoarthrosis, who had verified chronic gastritis in the anamnesis (50 males and 72 females), aged from 42 to 64. Control group included 40 patients with osteoarthrosis without gastroduodenal zone pathology in the anamnesis. For arthralgia relief, patients were prescribed to intake meloxicam (average dose - 12.5±1.39 mg/day) or nimesulide (average dose - 150±14.91 mg/day). Results: It was determined that prescription of selective NSAIDs (meloxicam and nimesulide) raised the risk of NSAIDs gastropathy/dyspepsia in 2.9 times (P<0.03) in patients with chronic gastritis in the anamnesis than in patients without associated gastroduodenal zone pathology. Atrophy of gastric mucous membrane was associated with higher risks (P>0.05) of erosive gastropathy. Conclusions: With the purpose of gastropathy prevention upon taking NSAIDs, patients with chronic gastritis in the anamnesis are recommended to undergo gastroprotective therapy.

The role of high sensitive C-reactive protein and histopathological evaluation in chronic gastritis patients with or without Helicobacter pylori infection.

Purpose To evaluate serum levels of high-sensitivity C-reactive protein (hs-CRP) in chronic gastritis patients to predict Helicobacter pylori (HP) infection, inflammatory activity, and precancerous lesions. Methods A total of 811 patients with upper gastrointestinal symptoms and histopathological diagnosis of chronic gastritis were enrolled in the study. On endoscopy, five gastric biopsies were taken according to Modified Sydney protocol, which were stained with hematoxylin & eosin and Giemsa ResultsHP infection was found in 28.6% of patients, being significantly more common in specimens with acute and chronic inflammatory activity. Mucosal atrophy, intestinal metaplasia, and dysplasia were found in 20.2%, 18.8% and 2.7% of biopsy specimens. Mean hs-CRP was 1.9±1.6 mg/dl for males and 2.2±1.9 mg/dl for females. hs-CRP average were significantly higher in patients with severe acute inflammation (p:0.049), in patients with severe chronic inflammation (p:0.015) and in those with HP (p: 0.001) . The severity of HP infection increased significantly with the increased degree of acute inflammation, chronic inflammation and hs-CRP level (p=0.001 for both). Conclusion Serum hs-CRP level increases in patients with chronic gastritis, it could be an indicator of severity of acute or chronic mucosal inflammation, and presence of HP infection. Therefore, hs-CRP may aid the diagnosis of chronic gastritis, but it is not associated with pre-cancerous lesions.

Immunohistochemical evaluation of P53 and Ki67 in biopsy samples of gastritis and gastric cancer patients

Background: Chronic gastritis (CG) is an inflammatory process that can lead to gastric cancer and Helicobacter Pylori (H. Pylori). In this study, immunohistochemical evaluation of P53 and Ki67 in biopsy samples of CG and gastric carcinoma patients with and without H. Pylori infection was investigated. Methods: From 82 archived paraffin blocks, 42 blocks were selected for CG group and 40 as the gastric cancer group. All CG and gastric cancer cases were subdivided into H. Pylori positive and negative subgroups. Monoclonal antibodies specific for Ki67 and P53 were used for immunohistochemical staining. Results: The results showed that differences of Ki67 and P53 expressions were statistically significant among patients with CG gastritis and gastric cancer (P 0.05). Conclusions: The present study proposed that P53 and Ki67 expressions changed in gastric cancer compared to the CG specimens. It seems that overexpression of these biomarkers probably has important roles in the route of carcinogenesis. Our results suggested that these overexpressions were not associated with H. Pylori infection. Further studies with larger sample size are needed in this field.

Blood flow differences in cun-kou (radial) artery and anterior tibial artery: normal people vs patients with chronic gastritis

OBJECTIVETo explore the blood flow differences in cun-kou (radial) artery and anterior tibial artery between normal people and patients with chronic gastritis METHODSUsing doppler ultrasonography, blood flow charts [peak systolic velocity (VP), maximum blood flow velocity in diastole (VD), mean blood flow velocity (VM), pulse index (PI), resistance index (RI), blood vessel diameter (D), vessel volume (SV), circulation blood flow periodic time (ET)] measured by at cun-kou (radial) artery and anterior tibial artery in normal group (n = 30) and chronic gastritis group (n = 30) in department of ultrasound, Beijing Anzhen hospital, capital medical university. RESULTSIn the doppler flow charts of the normal group, there were statistically significant differences in VD, VM, D, RI, SV, ET between cun-kou artery and anterior tibial artery (P < 0.05), and there were more statistically significant differences in PI between cun-kou artery and anterior tibial artery (P < 0.01). In the comparison of doppler flow charts in the chronic gastritis group, there were statistically significant differences in VD, VM, D, RI, SV, ET between cun-kou artery and anterior tibial artery (P < 0.05), and there were more statistically significant differences in PI between cun-kou artery and anterior tibial artery (P < 0.01). In the comparison of doppler flow charts between normal group and chronic gastritis group, there were statistically significant differences in SV, ET between normal group and chronic gastritis group (P < 0.05), and there were more statistically significant differences in PI between normal group and chronic gastritis group (P <0.01). CONCLUSIONBased on the differences in doppler flow charts between the normal group and the chronic gastritis group, the doppler flow charts of cun-kou artery and anterior tibial artery were not only proved to be significantly different, but also provided quantitative objective indexes for the study of cun-kou artery and anterior tibial artery doppler flow charts of normal people and chronic gastritis patients. This study also proves that the doppler flow chart of the anterior tibial pulse is of great significance for the diagnosis of chronic gastritis.

Development and validation of quality of life instruments for chronic diseases-Chronic gastritis version 2 (QLICD-CG V2.0).

Quality of life is an important outcome indicator to evaluate whether treatment is successful or not. Chronic gastritis leads to ongoing deterioration of subjectively perceived quality of life. There are several generic measures, but they are not developed particularly to assess chronic gastritis problems. The Quality of Life Instruments for Chronic Diseases—Chronic Gastritis (QLICD-CG V2.0) questionnaire is a 39-item, multi-dimensional, self-report instrument to assess chronic gastritis patients’ perception of their health related quality of life in four domains. The instrument was developed in China. The current study aimed to evaluate the psychometric properties of the QLICD-CG V2.0. 194 patients with chronic gastritis were enrolled from 4 hospitals in China. The QLICD-CG V2.0 was administered to patients by trained research assistants. In addition, their demographic characteristics were also recorded. The psychometric testing included construct validity, convergent validity, discriminant validity, test-retest, and responsiveness. The results showed good internal consistency and acceptable floor and ceiling effects (Cronbach’s alpha range from 0.80 to 0.93). CFA showed that the instrument structure has a reasonable fitness (RMSEA = 0.063, 95%CI = [0.057 0.079], CFI = 0.93, GFI = 0.95, SRMR = 0.028). The convergent validity was considered appropriate, with 38 of the 39 items correlated stronger with their assigned scale than a competing scale, except for GPS1. Known groups comparisons showed that the QLICD-CG V2.0 discriminated well between subgroups on the basis of gender, marriage status, and economy status, thus providing evidence of discriminative validity. Convergent validity testing revealed that the QLICD-CG V2.0 domain scores correlated significantly with SF-36 dimension scores, which ranged from 0.21 to 0.58. Test-retest coefficients were satisfactory. A majority of intraclass correlation coefficients were above 0.70, except the psychological domain (0.60) and the items of social support/security (0.61). Responsiveness was tested on 157 patients. Significant differences were found on all QLICD-CG V2.0 domains, between baseline responses and after a treatment, except for the items of appetite and sleep. Robust sensitivity to change was observed. The QLICD-CG V2.0 appears to be a valid and reliable instrument to measure QOL in chronic gastritis patients. Scores were reproducible.

Serum exosomes of chronic gastritis patients infected with Helicobacter pylori mediate IL-1α expression via IL-6 trans-signalling in gastric epithelial cells.

Emerging evidence has linked the exosomes to many immunological disorders, including infectious diseases. However, knowledge regarding the role of exosomes in Helicobacter pylori infection is limited. Here, we show that serum exosomes from chronic gastritis patients with H. pylori infection (Hp exosomes) stimulate the expression of the soluble interleukin (IL)-6 receptor (sIL-6R), which is involved in IL-6 trans-signalling in gastric epithelial cells. Interestingly, sIL-6R up-regulates expression of the proinflammatory cytokine IL-1α, and the neutralization of sIL-6R suppresses IL-1α secretion. Thus, Hp exosomes regulate IL-1α expression via sIL-6R-mediated IL-6 trans-signaling. Altogether, this study reveals a novel perspective in which exosomes play a vital role in immunological mechanisms during H. pylori infection.

Role of screening colonoscopy for colorectal tumors in Helicobacter pylori-related chronic gastritis with MDM2 SNP309 G/G homozygous: A prospective cross-sectional study in Thailand.

Helicobacter pylori infection is a risk factor for gastric cancer and colorectal cancer (CRC). MDM2 SNP309 G/G homozygosity is known to be the genetic background that influences the severity of inflammation in the gastric mucosa, and it corresponds to CRC development. We examined the role of screening colonoscopy in H. pylori-related chronic gastritis and the association of patients who have MDM2 SNP309 G/G homozygosity and advanced colorectal neoplasia (CRN) susceptibility. A prospective cross-sectional study was used to investigate H. pylori-related gastritis in 331 consecutive asymptomatic patients who had MDM2 SNP309 G/G homozygosity and who were enrolled from November 2014 to July 2017. The MDM2 SNP309 polymorphism was genotyped by real-time PCR hybridization probe assay. Totally, there were 331 patients with H. pylori-related gastritis, of whom 39 (8.76%) had advanced CRN. The H. pylori-positive group comprised 180 patients (54.36%). H. pylori infection was associated with advanced CRN (OR: 2.09, 95% CI: 1.56-2.80; p=0.01) and had an increased risk of advanced CRN (OR: 4.24, 95% CI: 1.76-5.21; p=0.01) after adjusting for confounding factors. Patients with H. pylori infection had a significantly increased risk of high-grade dysplasia or invasive adenocarcinoma (OR: 2.96, 95% CI: 1.48-4.17; p=0.03). Chronic gastritis patients infected with H. pylori and who had MDM2 SNP309 G/G homozygosity had an increased risk of advanced CRN, particularly high-grade dysplasia including invasive adenocarcinoma. Screening colonoscopy in these patients might benefit colorectal polyp diagnosis and prevention and early CRC treatment in the Thai population.

Effect of Huazhuo Jiedu formula on HIF-1α, VEGF, and PTEN in chronic erosive gastritis patients with syndrome of accumulation of turbidity and toxicity

To observe the clinical efficacy of Huazhuo Jiedu formula in treating chronic erosive gastritis (CEG) patients with syndrome of accumulation of turbidity and toxicity, explore its mechanism by observing the changes in expression levels of hypoxia inducible factor (HIF-1α), vascular endothelial growth factor (VEGF) in serum and gastric mucosa tissues after treatment, and provide theoretical basis for the clinical application of Huazhuo Jiedu formula in treating chronic erosive gastritis. All 70 patient of CEG were randomly divided into control group and treatment group, 35 cases in each group. The patients in control group received Alatan Wuwei Wan, bid, 1 bag/time; while the patients in treatment group were given with Huazhuo Jiedu formula, 1 dose/day. The course of the treatment was 6 months in both groups. The changes in clinical symptoms, gastroscopic signs, pathology and the expression levels of HIF-1α, VEGF, and phosphatase and tensin homolog deleted on chromosome ten (PTEN) in serum and gastric mucosa tissues were observed in both groups. The results showed that treatment group was better than control group in clinical efficacy, gastroscopic efficacy and pathological effect after treatment (P<0.05); the levels of HIF-1α and VEGF in serum of treatment group were lower than those in the control group after treatment (P<0.05), while the level of PTEN in serum of treatment group was higher than that in the control group after treatment (P<0.05); the levels of HIF-1α and VEGF in gastric mucosa tissues in the treatment group were lower than those in the control group after treatment, while the level of PTEN in gastric mucosa tissues in treatment group was higher than that in the control group after treatment (P<0.05), with statistically significant differences between these two groups (P<0.05). Huazhuo Jiedu formula can improve the clinical symptoms, gastroscopic signs and pathological conditions in CEG patients with syndrome of accumulation of turbidity and toxicity, and the mechanism may be associated with decreasing the expression level of HIF-1α, VEGF and increasing the expression level of PTEN.

P-088 - Genetic polymorphisms and PG1/PG2 and G17 levels can predict gastric carcinoids in autoimmune atrophic chronic gastritis patients

P-088 - Genetic polymorphisms and PG1/PG2 and G17 levels can predict gastric carcinoids in autoimmune atrophic chronic gastritis patients

Psychological distress in patients with chronic atrophic gastritis: the risk factors, protection factors, and cumulative effect

This study aimed to examine the psychological status among Chronic Atrophic Gastritis (CAG) patients and to find the cumulative effects of risk and protective factors. A sample of 101 CAG patients completed the investigation. Hierarchical linear regression was used to find risk and protective factors, and examine the cumulative effects in risk factor index (RFI) and protective factor index (PFI). Results showed that nine symptoms from SCL-90-R were severer among CAG patients than those in adult norm. Risk factors including positive family history of cancer and higher negative life events could predict higher GSI (β = 0.206, p = 0.023; β = 0.398, p < 0.001; R² = 0.203); more household resistant, positive coping and stronger resilience were protective factors and could predict GSI negatively (β = -0.188, p = 0.020; β = -0.350, p = 0.012; β = −0.066, p = 0.621; R² = 0.190). The GSI was positively correlated with RFI (β = 0.338, p < 0.001; R² = 0.113) and negatively related to PFI (β = −0.378, p < 0.001; R² = 0.133). In conclusion, CAG patients suffered from various psychological distress, and the protective factors should be enhanced cumulatively to protect against psychological distress.

Levels of malondialdehyde in the gastric juice: Its association with Helicobacter pylori infection and stomach diseases.

Helicobacter pylori (H.pylori) infection causes elevation of lipid peroxidation product malondialdehyde (MDA) and this association may be due to the bacterium causing reactive oxygen species-mediated damage to DNA in the gastric epithelium. The aim of this study was to investigate the gastric juice MDA levels in relation to H.pylori infection and associated gastric diseases. Gastric juice samples were obtained from 117 patients undergoing endoscopy, and gastric juice MDA levels were determined by high-performance liquid chromatography (HPLC) system. We compared the MDA levels between patients with and without H.pylori infection and assessed the differences of MDA levels between chronic gastritis, gastric intestinal metaplasia, and gastric cancer postsurgical resection. Malondialdehyde levels in gastric juice were significantly higher in chronic gastritis patients with H.pylori infection than in those without H.pylori infection (P<.0001). In patients without H.pylori infection, patients with gastric intestinal metaplasia and gastric cancer postsurgical resection had significantly higher gastric juice MDA level than patients with chronic gastritis. As a whole, patients with gastric intestinal metaplasia and gastric cancer postsurgical resection also had significantly higher MDA levels in gastric juice as compared to patients with chronic gastritis (P<.01). However, the difference of gastric juice MDA levels between gastric intestinal metaplasia and gastric cancer postsurgical resection was not significant. Malondialdehyde in gastric juice could be used as a potential diagnostic biomarker for H.pylori infection and associated gastric diseases. The gastric juice MDA levels increased proportionally with the severity of gastric diseases.

Subtypes of chronic gastritis in patients with celiac disease before and after gluten-free diet.

Celiac disease (CD) often manifests with dyspeptic symptoms and chronic gastritis is a common finding. To evaluate the frequency of lymphocytic gastritis (LG), chronic active gastritis (CAG), and chronic inactive gastritis (CIG) in patients with CD, before and after gluten-free diet (GFD). A five-year prospective study including all consecutive patients with a new diagnosis of CD was conducted. Gastric and duodenal biopsy specimens taken both at the time of the CD diagnosis and at the first endoscopic control after 18-24 months on GFD were evaluated. 213 patients with CD were enrolled. At the time of the diagnosis, 42 patients (19.7%) showed normal gastric mucosa, 34 (15.9%) LG, 67 (31.5%) CAG, and 70 (32.9%) CIG. Out of the 34 patients with LG, all were Helicobacter pylori negative and the majority of them showed an improvement both of gastritis (94.1%) and duodenal lesions (82.3%) after GFD. GFD did not show significant effects on CAG and CIG. LG is present in 16% of CD patients, it is not associated with H. pylori infection, and it improves after GFD. Both CAG and CIG are also frequently associated with CD, but fail to respond to a GFD.

FLOW MEDIATED DILATION AND CAROTID INTIMA MEDIA THICKNESS IN PATIENTS WITH CHRONIC GASTRITIS ASSOCIATED WITH HELICOBACTER PYLORI INFECTION.

Endothelial dysfunction is one of the early stages of vascular diseases. The aim of this study was to investigate the endothelial dysfunction markers in patients with chronic gastritis associated with Helicobacter pylori (H. pylori) infection. By a cross sectional study, basic and clinical information of 120 participants (40 patients with positive H. pylori infection, 40 patients with negative H. pylori infection and 40 healthy people) were analyzed. Carotid intima media thickness and flow-mediated dilation levels were measured in all patients and controls. Soluble vascular cell adhesion molecule-1 (sVCAM-1) and intercellular adhesion molecule-1 (ICAM-1) were measured with Elisa for all subjects. IgG level was assessed in chronic gastritis patients. The flow-mediated dilation level in patients with positive H. pylori infection (0.17%±0.09) was significantly lower than those with negative H. pylori infection (0.21% ±0.10, P<0.05) and compared to the control group (0.27% ±0.11, P<0.05). Carotid intima media thickness level in patients with positive H. pylori infection (0.58±0.13 mm) was significantly higher than those with negative H. pylori infection (0.48±0.32 mm, P<0.05) and compared to the control group (0.36±0.44mm, P<0.05). The mean level of sICAM-1 in positive H. pylori infection group (352.16±7.54 pg/mL) was higher than negative H. pylori infection group (332.64±8.75 pg/mL =0.75) and compared to the control group (236.32±12.43 pg/mL, P<0.05). A direct relationship was revealed between flow-mediated dilation and carotid intima media thickness changes and between sICAM-1 and sVCAM-1 associated with the level of H. pylori IgG in chronic gastritis. The levels of flow-mediated dilation, carotid intima media thickness and sICAM-1 were higher among patients with positive H. pylori infection. Patients with chronic gastritis associated with H. pylori infection are at risk of endothelial dysfunction due to flow-mediated dilation and carotid intima media thickness abnormalities and increased level of sICAM-1 and sVCAM-1.

Genetic variants of Helicobacter pylori type IV secretion system components CagL and CagI and their association with clinical outcomes

BackgroundHelicobacter pylori infection is associated with risk for chronic gastritis (CG), gastric ulcer (GU), duodenal ulcer (DU), and gastric cancer (GC). The H. pylori Cag type IV secretion system (TFSS) translocates the virulence factor cytotoxin-associated gene A protein into host cells and plays an important role in initiating gastric carcinogenesis. The CagL and CagI proteins are components of the TFSS. The Arg-Gly-Asp (RGD) motif of CagL, and the six most distal C-terminal amino acids (Ser-Lys-Ile-Ile-Val-Lys, and Ser-Lys-Val-Ile-Val-Lys) of CagL and CagI are essential for TFSS adhesion to host cells. Additionally, the CagL variant Tyr58Glu59 was previously shown to be associated with GC patients.ResultsWe isolated 43 H. pylori isolates from 17 CG, 8 GU, 8 DU, and 10 GC patients in Southeast Asia. Total DNAs were extracted and sequenced with MiSeq. H. pylori strain ATCC 26695, which was isolated from CG patients, was used as a reference. We examined the full sequences of H. pylori cagL and cagI using whole-genome sequencing (WGS), and analyzed whether single nucleotide variants and amino acid changes (AACs) correlated with adverse clinical outcomes. Three isolates were excluded from the analysis due to cagPAI rearrangements. CagL RGD motifs were conserved in 39 isolates (97.5%). CagL-Glu59 and Ile234 in the C-terminal motif were more common in 10 H. pylori isolates from GC patients (p < 0.001 and p < 0.05, respectively). When 5 Vietnamese isolates from GC patients were excluded, CagL-Glu59 still remains significant (p < 0.05), but not Ile234. CagL-Tyr58 was seen in only one isolate. The CagI C-terminal motif was completely conserved across all 40 isolates, and there were no significant AACs in CagI.ConclusionsUsing WGS, we analyzed genetic variants in clinical H. pylori isolates and identified putative novel and candidate variants in uncharacterized CagL and CagI sequences that are related to gastric carcinogenesis. In particular, CagL-Glu59 has the possible association with GC.

Regulation of Gastric Carcinogenesis by InflammatoryCytokines.

Chronic inflammation caused by infection with Helicobacter pylori and autoimmune gastritis increases an individual’s risk of developing gastric cancer. More than 90% of gastric cancers are adenocarcinomas, which originate from epithelial cells in the chronically inflamed gastric mucosa. However, only a small subset of chronic gastritis patients develops gastric cancer, implying a role for genetic and environmental factors in cancer development. A number of DNA polymorphisms that increase gastric cancer risk have mapped to genes encoding cytokines. Many different cytokines secreted by immune cells and epithelial cells during chronic gastritis have been identified, but a better understanding of how cytokines regulate the severity of gastritis, epithelial cell changes, and neoplastic transformation is needed. This review summarizes studies in both human and mouse models, describing a number of different findings that implicate various cytokines in regulating the development of gastric cancer.

The Progression of Atrophic Gastritis to Gastric Cancer: A Retrospective Study in 61,810 Cases

Background and Aim: Chronic atrophic gastritis is a known precancerous lesion for gastric cancer, with an overall 5-year survival less than 20%. However, there is few new data describing the progression rate from atrophic gastritis (AG) to gastric cancer in China. We retrospectively analyzed the risk of gastric cancer among patients with AG and aimed to determine the accuracy of endoscopy diagnosis of AG in China. Methods: Clinical features and endoscopic profiles of chronic atrophic gastritis patients from Wenzhou People’s Hospital between January 2006 and December 2016 were analyzed retrospectively. Results: There were 61,810 cases analyzed retrospectively. 3641 cases of atopic gastritis were diagnosed by endoscopy, in which 1704 cases were confirmed by pathological biopsy; the diagnostic coincidence rate was 46.80%; 2631 cases were diagnosed as atrophic gastritis by pathological biopsy, in which 927 cases were ignored by endoscopy; the miss diagnosis rate was 35.23%. The progression rate from chronic atrophic gastritis and non-atrophic gastritis to gastric cancer respectively was 0.79% and 0.43%. The rate of intestinal metaplasia in mild AG, moderate AG and severe AG respectively was 75.63%, 86.18% and 90.32%, 3.42%, 6.27% and 5.16% about dysplasia in three different degree atrophic gastritis. Conclusions: Endoscopy diagnosis and pathology diagnosis of AG were statistically different. Patients with atrophic gastritis have higher risk of developing into gastric cancer, but it needs further investigation. Different degrees of atrophic gastritis have the different proportions of intestinal metaplasia and dysplasia.

The study of the correlation between helicobacter pylori infection and TCM syndrome of chronic gastritis in Yongwai district of Beijing

Objective To explore the correlation between Helicobacter pylori infection and TCM syndrome of chronic gastritis in Yongwai of Beijing. Methods A total of 202 patients with chronic gastritis in the Yongwai district of Beijing were detected with H.pylori infection and tested by the 13C-urea breath test. The patients were classified by the disease severity according to the results of gastroscope, and diagonsed with TCM Syndromee. The distribution characteristics and correlation of H.pylori infection and the TCM syndromes were analyzed. Results The chronic gastritis patients with atrophic gastritis showed the higher infection rate of 81.2%, among which the gastric antrum was most inflammatory areas with 80.7%. The patients with chronic gastritis with positive H.pylori always correlated with the TCM syndromes such as the blood stasis of stomach Luo and the dampness-heat of spleen and stomach. And among these two TCM syndromes, the frequency of the intestinal metaplasia and atypical hyperplasia were higher than other syndromes. Conclusions The H.pylori infection is the common cause of chronic gastritis, and it was always correlated with the TCM syndromes blood stasis of stomach Luo and the dampness-heat of spleen and stomach. Key words: Helicobacter pylori; Chronic gastritis; The Chinese medicine dialectical

Comparison of Helicobacter pylori Detection Using Immunohistochemistry and Giemsa and Its Association with Morphological Changes in Active Chronic Gastritis

Background: Gastritis is an inflammation of the gastric mucosa as a response to infection or irritation of the gaster. The most common aetiology of chronic gastritis is Helicobacter pylori (H. pylori) infection. Presence of H. pylori is associated with the occurrence of inflammation, atrophy, and intestinal metaplasia. In terms of morphology, H. pylori is known in 2 forms, which are rod-shaped and coccoid-shaped. Coccoid-shaped bacteria are difficult to detect using Giemsa staining. Therefore, immunohistochemistry staining of H. pylori and evaluation of the sensitivity of coccoid-shaped of H. pylori are needed.Method: Cross-sectional study on 90 biopsy tissues of chronic gastritis patients in year 2015 and 2014, which included 30 Giemsa cases with positive H. pylori, 30 cases of active chronic gastritis with negative H. pylori but coccoid-shaped was found, and 30 non-active chronic gastritis, were subsequently stained with immunohistochemistry staining of H. pylori.Results: Expression of coccoid-shaped H. pylori in active chronic gastritis was significantly different (p 0.05) in immunohistochemistry staining. There was a significant difference between active chronic gastritis with positive H. pylori and negative H. pylori in immunohistochemistry staining with degree of inflammation. Sensitivity and specificity test between Giemsa and immunohistochemistry staining showed sensitivity of 65% and specificity of 100%.Conclusion: Immunohistochemistry staining in active chronic gastritis was more sensitive compared to Giemsa staining in detecting H. pylori, particularly the coccoid-shaped bacteria.

Functional dyspepsia through chronic gastritis in patients with marfan syndrome and marfan-like states

The article presents the results of clinical and endoscopic examination of patients (n = 89; 33 ± 9,3 years; F : M/23 : 66) with confirmed chronic gastritis associated with hereditary disorders of connective tissue (Marfan syndrome phenotype and Marfan-like states) in order to examine the relationship associated HDCT as monogenic diseases - Marfan syndrome, as well as related pathology in the form of Marfan-like states with the clinical manifestations of functional dyspepsia and endoscopic picture of H. pylori infection in chronic gastritis. Diagnosis of Marfan syndrome was based on the revised criteria of Ghent. For the differential diagnosis with other “related” diseases of the connective tissue used syndrome diagnostic criteria of hypermobility of joints, Ehlers - Danlos syndrome. Evaluation of complaints and diagnosis of functional dyspepsia was based on the criteria of Rome III (2006) and the Florence Agreement. Evaluation of the symptoms of functional dyspepsia showed different from control (p &lt; 0,05) lower frequency of epigastric pain syndromes and postprandial distress syndrome in a group with Marfan syndrome not associated with H. pylori infection and, at the same time, does not correlate with available in this group of patients a high frequency of precancerous lesions in the gastric mucosa. Frequent endoscopic manifestations associated with the HDCT served esophagitis gastroduodenal motility disorders and the development of GERD with catarrhal esophagitis (p &lt; 0,05) and the absence of erosive and ulcerative lesions of gastroduodenal mucosa (p &lt; 0,05). The findings strongly indicate the absolute necessity of endoscopic and morphological screening regardless of the presence of gastrointestinal complaints in patients with associated HDCT.

Bacillus as a potential diagnostic marker for yellow tongue coating.

Observation of tongue coating, a foundation for clinical diagnosis and treatment in traditional Chinese medicine (TCM), is a major indicator of the occurrence, development, and prognosis of disease. The biological basis of tongue diagnosis and relationship between the types and microorganisms of tongue coating remain elusive. Thirteen chronic erosive gastritis (CEG) patients with typical yellow tongue coating (YTC) and ten healthy volunteers with thin white tongue coating (WTC) were included in this study. Patients were provided a 2-course targeted treatment of a herbal medicine Ban Xia Xie Xin decoction, traditionally prescribed for CEG patients with YTC, to evaluate the relationship between tongue coating microbiota and diagnosis of CEG with typical YTC. The tongue coating segregation structure was determined using Illumina Miseq sequencing of the V4–V5 region of the 16S ribosomal RNA gene. Bacillus was significantly observed only in CEG patients with YTC, but not in patients who received the decoction. YTC (n = 22) and WTC (n = 29) samples were collected for bacterial culturing to illustrate the relationship between Bacillus and YTC. The Bacillus positivity rate of YTC samples was 72.7%; Bacillus was not observed in WTC samples. In conclusion, Bacillus was strongly associated with YTC.