We previously reported that MatSep (3 hours/day from days 2‐14) induces exaggerated angiotensin II (AngII)‐induced vasoreactivity with decreased nitric oxide (NO) buffering capacity in aortae of adult rats compared to control rats (normally‐reared littermates). Also, chronic AngII infusion produced exaggerated hypertension in MatSep rats. The aim of this study was to investigate whether MatSep male and female rats display exaggerated vascular reactivity of resistance arteries. Third‐order mesenteric arteries from adult rats (n=5‐6 per group) were isolated and mounted on wire myographs. Concentration‐response curves were generated for phenylephrine or endothelin and normalized to maximal potassium chloride‐induced constriction. Additionally, we tested acetylcholine and sodium nitroprusside‐induced relaxation. No differences in EC50 or %maximum constriction/dilation were observed between MatSep and control rats. Curves in the presence of L‐NAME revealed similar NO buffering capacity in MatSep and control rats. No sex differences were observed across the study. These data suggest that the abnormal vascular phenotype due to MatSep involves large conduit arteries, sparing smaller resistance arteries. However, resistance arteries are known to play an important role in blood pressure control, and thus, further investigation of these arteries in response to chronic AngII infusion is warranted.Grant Funding Source: Supported by NIH Grants: P01 HL69999, K99 HL111354