In this work, a simple and effective strategy was proposed based on the combination of liquid chromatography diode array detection (HPLC-DAD) with second-order calibration method for fast and simultaneous determination of twelve preservatives in a variety of cosmetic products. The twelve preservatives were completely eluted out within 10.5 min under a simple sample preparation step. The proposed method shows “second-order advantage”, which enables one to accurately quantify the twelve preservatives in complex cosmetics in the presence of co-eluting chromatographic peaks and diverse unknown interferences. As a result, the consumption of organic solvents was markedly reduced and analysis time was greatly shortened. All target analytes showed good linearity in the calibration range with root-mean-square error of calibration (RMSEC) less than 0.84. The average spiked recoveries for all target analytes were in the range of 93.9%-110.5% with standard deviations less than 9.3%. In addition, analytical figures of merit were calculated to validate the performance of the proposed method. All these results demonstrated that the developed strategy was fast, green and accurate. Consequently, it could be used as an attractive alternative for the determination of multiple preservatives in complex cosmetic samples.