Chonburi Province Research Articles

Screening and application of microsatellite markers for genetic diversity analysis of captive eastern sarus crane Grus antigone sharpii Blanford, 1895 in Thailand.

The eastern sarus crane, Grus antigone sharpii, is distributed in the Indochina area, though it has become extinct in Thailand. The Thai government has tried to repopulate the cranes using wild individuals from Cambodia as initial breeding stock. Although captive breeding can reintroduce species back into the wild, the genetic diversity of the population is also important. This study aimed to screen microsatellite markers to investigate the genetic diversity of G. a. sharpii from two breeding facilities in Thailand and to assess its potential for future conservation programs. Eighteen microsatellite loci isolated from whooping crane (G. americana) and blue crane (Anthropoides paradisea) were screened in all captive reared cranes from Khao Kheow Open Zoo (n = 11) and Bangpra Water Bird Breeding Station (n = 17), Chonburi Province. Of 18 loci analyzed, 14 were found to be polymorphic, and 3 loci were in linkage disequilibrium. Apparent deviation from Hardy-Weinberg equilibrium was observed only at locus Gram8, resulting from the presence of a null allele. The average expected and observed heterozygosities from 10 loci analyzed are 0.61 and 0.64, respectively. The overall fixation index suggested that cranes from both facilities shared many common alleles (FST = 0.023, p < 0.05); in addition, Bayesian clustering analysis implied that cranes from two breeding facilities are homogeneous (K = 1). Our findings reveal high level of genetic diversity of the captive crane population in Thailand and suggest that the breeding stocks may be suitable for ensuring a sustainable breeding program in the future.

Fuel Station Capacity on Major Arterial Road in Thailand: A Case Study in Chonburi Province, Thailand

Fuel Station Capacity on Major Arterial Road in Thailand: A Case Study in Chonburi Province, Thailand

Participatory capacity building for improving quality of childcare centers in Thailand.

Childcare centers (CCCs) with good quality standards can be effective in reducing the risk of diseases being easily spread from person to person. The aim of the present study's program, adapted from a method used by the United Nations Development Program, was to increase the capacity of local administrators(s) and heads of CCCs to improve quality standards. This study was quasi-experimental, with a one group pretest-post-test design. In this study the authors describe the effects and impacts of the program in Chonburi Province in eastern Thailand. Six LAs and 48 CCC heads were trained regarding (1) knowledge of the Thai Department of Health quality standards, (2) implementation and assurance and (3) program evaluation. The program consisted of three sequential participatory workshops. Effects at the center level were increased overall knowledge of quality standards of CCCs (QCCC), and developed skills of improvement plans. The impact at the center level was CCCs achieving the QCCC. At the child level it was the reduction in the period prevalence of three diseases and two symptoms. The significant (p<0.05) effects and impacts at the center level were increased overall knowledge of QCCC in LAs and CCC heads and increased overall managerial skills of CCC heads. At the child level, the period prevalence of chickenpox and diarrhea symptom were reduced. Participatory capacity building is an appropriate way to enhance the managerial skills of LAs and heads of CCCs for improving quality of CCCs to meet the local authority and the Ministry of Public Health quality standards.

Human capital accumulation of rural female migrants and occupational mobility at destination area: A case study of Chonburi

Occupational mobility influences the social status of female migrants who have migrated from rural to urban areas. Occupational progression leads to social class mobility. The purpose of this research was to examine human capital accumulation and occupational mobility among female migrants in the destination area. Using qualitative methodology, in-depth interviews were conducted with 26 female migrants from the rural Northeast of Thailand to Chonburi Province and with 6 key informants between October 2015 to January 2016. The data were analyzed by analytic description. The findings indicated that the female migrants from the rural Northeast of Thailand relied on multiple ways of accumulating human capital for occupational mobility, namely: 1) learning on the job, resulting in an advancement from working as a cook’s assistant, waitress, or assembly line worker (Level 1 skills) to working as a tourist guide, noodle vendor, or beauty parlor/general store operator (Level 2 skills);2) attending adult education, which led to occupational advancement from being a domestic worker (Level 1 skills) to an accountant in a private company (Level 2 skills);3) studying at a formal educational institution, which helped a domestic worker to assume a position as a clerk at a state agency (Level 2 skills);4) vocational short course training, which led in occupational advancement from being an assembly line worker to opening a sewing shop (Level 2 skills). In conclusion, the findings indicated that these female migrant’s way of human capital accumulation influenced their occupational mobility, but it also depended on their personal characteristics.

Natural infection of questing ixodid ticks with protozoa and bacteria in Chonburi Province, Thailand

Ixodid ticks are important vectors of tick-borne disease agents affecting humans and animals, with wildlife often serving as important reservoirs. This study examined protozoal and bacterial infection in questing ticks in forest habitats in Chonburi Province, Thailand in 2015, using PCR and DNA sequencing techniques. A total of 12,184 ticks were morphologically identified to species and a subset of ticks were confirmed by PCR, targeting the tick mitochondrial 16S rRNA gene. Tick species collected included Haemaphysalis lagrangei (92.8%), H. wellingtoni (0.1%), and Rhipicephalus microplus (7.0%). In total, 419 tick pools [ELM(1] [ST2] were examined by PCR amplification of a fragment of the 18S rRNA gene of Babesia and Theileria species, and the 16S rRNA gene of bacteria in the family Anaplasmataceae. Results revealed a tick infection rate for the tick pools of 57.0% (239/419) including four pathogens and one bacterial symbiont. The highest infection rate in H. lagrangei, H. wellingtoni, and R. microplus pools was recorded for Anaplasma spp. at 55.6% (233/419) including three Anaplasma species genotype groups Anaplasma spp. closely related to A. bovis, A. platys, and unidentified Anaplasma spp. Theileria spp. showed a lower infection rate in H. lagrangei at 4.3% (18/419) with three Theileria species genotypes closely related to T. cervi, T. capreoli, and unidentified Theileria spp. Only 0.2% (1/419) of H. lagrangei pools contained Babesia spp., Ehrlichia spp., or Wolbachia spp. [ELM(3] [ST4] These findings provided information on tick species in wildlife habitats and detected protozoa and bacteria in the ticks. The results suggest that these tick species are possible vectors for transmitting tick-borne disease agents in Thailand wildlife habitats.

Longitudinal study of age-specific pattern of coronavirus infection in Lyle\u2019s flying fox (Pteropus lylei) in Thailand

BackgroundBats are natural reservoirs for several highly pathogenic and novel viruses including coronaviruses (CoVs) (mainly Alphacoronavirus and Betacoronavirus). Lyle’s flying fox (Pteropus lylei)‘s roosts and foraging sites are usually in the proximity to humans and animals. Knowledge about age-specific pattern of CoV infection in P. lylei, prevalence, and viral shedding at roosts and foraging sites may have an impact on infection-age-structure model to control CoV outbreak.MethodsP. lylei bats were captured monthly during January–December 2012 for detection of CoV at three areas in Chonburi province; two human dwellings, S1 and S2, where few fruit trees were located with an open pig farm, 0.6 km and 5.5 km away from the bat roost, S3. Nested RT-PCR of RNA-dependent RNA polymerase (RdRp) gene from rectal swabs was used for CoV detection. The strain of CoV was confirmed by sequencing and phylogenetic analysis.ResultsCoV infection was found in both juveniles and adult bats between May and October (January, in adults only and April, in juveniles only). Of total rectal swab positives (68/367, 18.5%), ratio was higher in bats captured at S1 (11/44, 25.0%) and S2 (35/99, 35.4%) foraging sites than at roost (S3) (22/224, 9.8%). Juveniles (forearm length ≤ 136 mm) were found with more CoV infection than adults at all three sites; S1 (9/24, 37.5% vs 2/20, 10%), S2 (22/49, 44.9% vs 13/50, 26.0%), and S3 (10/30, 33.3% vs 12/194, 6.2%). The average BCI of CoV infected bats was significantly lower than uninfected bats. No gender difference related to infection was found at the sites. Phylogenetic analysis of conserved RdRp gene revealed that the detected CoVs belonged to group D betacoronavirus (n = 64) and alphacoronavirus (n = 4).ConclusionsThe fact that CoV infection and shedding was found in more juvenile than adult bats may suggest transmission from mother during peripartum period. Whether viral reactivation during parturition period or stress is responsible in maintaining transmission in the bat colony needs to be explored.

Scrub Typhus Outbreak in Chonburi Province, Central Thailand, 2013.

Investigation of a scrub typhus outbreak in Thailand during September 2013 found that 9.1% of Thai soldiers and 11.1% of residents living in areas surrounding training sites had antibodies against the causative agent, Orientia tsutsugamushi. Sequence analysis of O. tsutsugamushi from rodents and chiggers identified 7 genogroups and 3 genotypes.

Factors Affecting the Knowledge on Local Wisdom for the Handicraft in Phanat Nikhom District, Chonburi Province, Thailand

The wicker handicraft is the local wisdom of the communities very essential for living. It represents the smart wisdom of local people in applying the things already existing in the communities to make the daily utensils beneficial for living. Besides, it can be seen that the wicker handicraft has existed for long time ago and has been continually developed by means of knowledge transfer from one generation to the following generations. The learning can be done by practicing, training, and telling. However, it lacks the system to record the information reflecting the learning accumulated and transferred from the past until present. Therefore, the knowledge transfer in the works is very important in maintaining such local wisdom. The results of study on the knowledge in the local wisdom works can be used in developing the process of knowledge transfer in the local wisdom of wicker handicraft more efficiently and sustainably. The researcher uses the mixed methodology which is the quantitative study to focus on finding the facts of information in numbers to confirm the correctness. The questionnaires are used for the data collection on 400 samples from the population in the municipal area of Phanat Nikhom District, Chonburi Province. The qualitative study is also conducted from the in-depth interview on 10 persons from the group of leaders and members. The data is analyzed by using the descriptive statistics including percentage, mean, standard deviation, and inferential analysis. The causal relationships are analyzed to find the relationships of factors of organization and the factors of knowledge in works. The research results reveal that the factors of organization in the leadership, technology, and communication including the organizational culture are all important to the knowledge in works. Such factors have direct influences on the effectiveness of development of local wisdom in handicraft. If it is supported, promoted, and developed in the knowledge process in the works which respond the organization’s demand appropriately. This can yield more efficiency and effectiveness. Therefore, these factors are necessary to be used in developing the knowledge in local wisdom for handicraft for the locality and community people to have continual development with the maximum efficiency.

Factors Affecting the Knowledge on Local Wisdom for the Handicraft in Phanat Nikhom District, Chonburi Province, Thailand

The wicker handicraft is the local wisdom of the communities very essential for living. It represents the smart wisdom of local people in applying the things already existing in the communities to make the daily utensils beneficial for living. Besides, it can be seen that the wicker handicraft has existed for long time ago and has been continually developed by means of knowledge transfer from one generation to the following generations. The learning can be done by practicing, training, and telling. However, it lacks the system to record the information reflecting the learning accumulated and transferred from the past until present. Therefore, the knowledge transfer in the works is very important in maintaining such local wisdom. The results of study on the knowledge in the local wisdom works can be used in developing the process of knowledge transfer in the local wisdom of wicker handicraft more efficiently and sustainably. The researcher uses the mixed methodology which is the quantitative study to focus on finding the facts of information in numbers to confirm the correctness. The questionnaires are used for the data collection on 400 samples from the population in the municipal area of Phanat Nikhom District, Chonburi Province. The qualitative study is also conducted from the in-depth interview on 10 persons from the group of leaders and members. The data is analyzed by using the descriptive statistics including percentage, mean, standard deviation, and inferential analysis. The causal relationships are analyzed to find the relationships of factors of organization and the factors of knowledge in works. The research results reveal that the factors of organization in the leadership, technology, and communication including the organizational culture are all important to the knowledge in works. Such factors have direct influences on the effectiveness of development of local wisdom in handicraft. If it is supported, promoted, and developed in the knowledge process in the works which respond the organization’s demand appropriately. This can yield more efficiency and effectiveness. Therefore, these factors are necessary to be used in developing the knowledge in local wisdom for handicraft for the locality and community people to have continual development with the maximum efficiency.

Network Management for Preventing and Resolving Human Trafficking Issues in Chonburi Province, Thailand

The objective of this research is to study, analyze, and synthesize network management for preventing and resolving human trafficking issues in human trafficking. A qualitative approach, including in-depth interview, group discussion, and network forum among 33 main informants, were used for data collection. Content analysis was bringing to apply afterwards. From the study, it has been found that network management for preventing and resolving human trafficking in Chonburi province are applied at both provincial and district levels. At the provincial level, the Provincial Office of Social Development and Human Security is the operational center that helps the affected persons, in collaboration with the police, the Provincial Office of Labor Protection and Welfare, the Provincial Office of Employment, and the Provincial Office of Public Health. In addition, training sessions are organized to educate workers and business owners. At the district level, the Provincial Office of Social Development and Human Security, the local police, and sub-district health promoting hospitals, with the chief district officer as the head of the working team. The suggestions include involving all agencies in the network in resolving human trafficking problems in order to unify the team and maximize the efficiency, improving human trafficking-related data organization system to make it more up-to-date and accessible to all relevant parties, expanding the collaboration to the public and other networks in the community to monitor and resolve human trafficking issues.

Collaborations and Design Development of Local Craft Products: Service Design for Creative Craft Community

Local craft product is one of the key elements in expressing different identities of local culture in different countries. Local craft communities in Thailand have quite unique skills in producing different craft using local materials and techniques. However, the craft production is later facing the problem of missing their own identity. The products are also unable fit to the demand of international market. This research is a result of the co-creation project on developing local craft products which is a part of the research on a service design for creative craft community: A case study of Phanusnikom district, Chonburi province, Thailand. The benefits of this study will stimulate the development and preservation of local craft products and communities in a more sustainable way. This study aims to explore the possible methods of how designers, local craftsmen, academic institutions and local business clusters can collaborate and raise new opportunities for the communities to become a creative craft space. The study has concentrated on a case study of Phanusnikom District, Chonburi Province, Thailand where most of the people are creating wickerwork from bamboo. The data collected within the research come from ethnographic fieldwork, which consists of basic methods such as site visits and interviews. The research also includes the concept of Service design thinking and tools, which can help to analyze the data into different sets of relationship between service users, service providers, and environment. Groups of product design students, local craftsmen, local business clusters, professional designers, and artists are co – creating new craft products. The results express throughout the process which emphasize on presenting the ability of local craftsmen beyond what they are expected; exchanging knowledge; creating a more comfortable collaboration between the stakeholders; and strengthen the local craft community for more future sustainable developments.

Cryopreservation of sperm from the coral Acropora humilis

Corals are sensitive to minute changes in their environments, and their continued existence is substantially threatened by the increasing number of destructive anthropogenic activities and unprecedented rates of climate change. Although cryopreservation has been successfully to preserve mammalian gametes for decades, coral cryopreservation was attempted for the first time less than 15 years ago, and freezing protocols exist for only a handful of coral species. The present study developed a cryopreservation protocol for the sperm of the common Indo-Pacific reef-builder Acropora humilis. Colonies of reefs of Sattahip Bay, Chonburi Province, Thailand were collected from 3 m depth with a mesh net during a spawning event. Immediately after collection, the sperm were isolated and subjected to a two-step freezing method featuring DMSO, polyethylene glycol, or methanol as the cryoprotectant. Viability and motility were assessed via a bioluminescence technique and a “computer-assisted semen analysis, and it was found that a 15-min equilibration with 2 M DMSO followed by cooling at 41.7 °C was the optimum cryopreservation protocol for A. humilis sperm. The post-thaw sperm achieved 45% fertilization success, and 35% of the fertilized eggs developed into blastopore larvae. The present optimized protocol can therefore facilitate the preservation of sperm for future propagation efforts of this species and provide an experimental platform for optimizing cryopreservation protocols for gametes of other scleractinian coral species.

A Survey of Metal Contamination in Blood Collection Tubes on Toxicology Assays

OBJECTIVE: To investigate metal contamination in lavender top blood collectiontubes (BCTs) coated with ethylenediaminetetraacetic acid (EDTA) from the Departmentof Occupational Health at hospitals in Chonburi Province.MATERIALS AND METHODS: One hundred and fifty lavender top blood collectiontubes (BCTs) that had been stored properly and were not expired from the Departmentof Occupational Health at 9 hospitals in Chonburi Province were collected. Deionizedwater which had already been screened for metal contamination was added to alllavender top BCTs. The samples were analyzed for metal contamination by inductivelycoupled plasma mass spectrometry (ICP-MS).RESULTS: The metal contamination in BCTs included aluminum (Al), cadmium (Cd),chromium (Cr), cobalt (Co), copper (Cu), manganese (Mn), nickel (Ni), lead (Pb), tin(Sn) and zinc (Zn). The first top 5 with highly contaminated median were zinc (20.39μg/L), aluminum (6.64 μg/L), copper (1.59 μg/L), nickel (1.14 μg/L), and lead (0.71μg/L). Aluminum and manganese were remarkable contamination when compared withthe reference values in humans. The contamination was different in each brand. However,arsenic (As), mercury (Hg), selenium (Se) and thallium (TI) were not found.CONCLUSION: This study showed that there was metal contamination in lavendertop BCTs. However, the laboratory errors can occur in several stages. Hence, it isimportant to review the validity and quality of the entire procedure prior to the adoptionof the outcomes. Many decontamination techniques were suggested, but they are notpractical. Therefore, royal blue top BCTs were recommended for blood collection formetal or trace element analysis.

Seasonal variation in nutritional composition and anti-proliferative activity of brown seaweed, Sargassum oligocystum

This study investigated the nutritional components and anti-proliferative activity of Sargassum oligocystum against an adriamycin-resistant human small cell lung carcinoma cell line (GLC4/Adr). The seaweed samples were collected from Nang Rong Beach, Chonburi Province, Thailand in February (the hot/dry season), May (the early monsoon season), August (the monsoon season) and December (the cool/dry season) of 2012. In general, the nutritional composition of S. oligocystum was high during the hot dry and early monsoon season. Additionally, four different types of extract were obtained: ethanolic extract (E1), lipophilic extract (E2), acidic extract (E3) and alkali extract (E4). The highest anti-proliferative activity was found in samples collected during the monsoon season. It is noteworthy that the E2 extract collected during the monsoon season was the most effective, with an IC50 (half maximal inhibitory concentration) value of 2.52 ± 0.54 μg mL−1. The anti-proliferative activity showed a positive correlation with vitamin E (α-tocopherol) in the extracts. However, there were no clear correlations between the total phenolic or fucoxanthin contents and anti-proliferative activity. These results indicate that S. oligocystum has potential as an alternative source for functional food or cancer treatments in the future.

Coastal debris analysis in beaches of Chonburi Province, eastern of Thailand as implications for coastal conservation

This study quantified coastal debris along 3 beaches (Angsila, Bangsaen, Samaesarn) in eastern coast of Thailand. Debris samples were collected from lower and upper strata of these beaches during wet and dry seasons. The results showed that Bangsaen had the highest average debris density (15.5m−2) followed by Samaesarn (8.10m−2), and Angsila (5.54m−2). Among the 12 debris categories, the most abundant debris type was plastics (>45% of the total debris) in all beach locations. Coastal debris distribution was related to economic activities in the vicinity. Fishery and shell-fish aquaculture activities were primary sources of debris in Angsila while tourism activities were main sources in Bangsaen and Samaesarn. Site-specific pollution control mechanisms (environmental awareness, reuse and recycling) are recommended to reduce public littering. Management actions in Angsila should focus on fishery and shell-fish culture practices, while Bangsaen and Samaesarn should be directed toward leisure activities promoting waste management.

Accumulation of domoic acid in marine organisms from Sriracha bay, Chonburi province, Thailand

Accumulation of domoic acid in marine organisms from Sriracha bay, Chonburi province, Thailand

Structural Equation Models for Building Awareness and Acceptance of Public Participation in Thailand’s Comprehensive Urban Planning Procedures

A comprehensive planning procedure is the key for urban development. It is considered as a highly concrete approach to enable the enforcement of a comprehensive urban plan, which indicates the future direction of urban development. This study aims to develop Structural Equation Modelling (SEM) to build perception and acceptance in civic society to participate in the comprehensive planning procedure of the SEM technique. Factors were identified that affect the perception and acceptance of public participation in urban planning procedures. The Baan Bueng District of Chonburi Province and the Khok Toom Community in Lopburi Province were selected as case studies because these areas were operating with comprehensive planning procedures at the time of the study. The study argued that planning model combines with participatory approach plays an important role in filling this gap. This method of planning procedures will need to be adapted with existing factors and influences in urban setting. A literature review of these factors was conducted to explain the empirical results that indicated most people lacked a knowledge and access to the information of urban planning to prepare for participation process. This study focuses on those residents who receive information or attitude to accept on a comprehensive urban planning procedures, which SEM is an instrument for analyzing development process in their areas and to participate in a comprehensive plan. From a preliminary analysis of the social-economic group with participation factors. The results showed that there is contras variation in negative with attitudes and accept of the urban planning procedures. In principle of a statistically significant result considering from the group’s participants in the planning procedures. The Respondents are volunteer group of community and elderly group, who had less education and unstable career prepared to the working group did not participate in any activities. Hence, people participation is the key in development of urban planning procedures, which stakeholders must participate in order to accept in the urban planning process. This forms of participation process in currently process is currently in progress, is considered at the first stages of participation concept and most abstract, affect in lower perception and acknowledgement of participation. In performing the many tasks required in this process the planning procedure is costly both in terms of processing time and budget. Local administrative agencies still need concrete decision-making tools and measures to overcome difficulties and obstacles.

Survey of the Risk Points for Alcohol Consumption of Youth Around the Higher Education Institutions in Eastern Thailand

This study was aimed to determine the risk factor related to the availability of shops that offered alcoholic beverages, which located near the university or college in provinces in Eastern Thailand. The cross-sectional survey was carried out with shops, bars, pubs, and restaurants where alcoholic beverages were available for consumption. The criteria used in selection of the places were the distances between the institutions and the selected places, where four distances were selected, i.e., 300, 500, 1000, and more than 1,000 meters. Data collection was done during March and May 2015, which ten institutions were selected. The record forms and photos were used as instruments. It was shown that the availability of the places that offered alcoholic beverages located near the higher education institutions was highest in Chachoengsao Province where the selected places were located within 300 meters from the institutions, followed by those in Chonburi Province. There were many illegal unregistered places where alcoholic beverages available located within the distance less than 300 meters away from the education institutions. Moreover, it was found that the risk factor for the alcohol consumption of youth around the education institutions was probably not depended on the distances among the places and the institutions, but it was related to the ability to access the places because students could travel to these places easily, where various places that sold alcoholic beverages were available, i.e., grocery, convenience stores, nightlife entertainment places, bars, and pubs.

Micromonospora sediminis sp. nov., isolated from mangrove sediment.

The novel actinomycete, strain CH3-3T, was isolated from mangrove sediment collected from Chonburi Province, Thailand. On the basis of its spore morphology and chemotaxonomic characteristics, the strain belonged to the genus Micromonospora. It contained meso-diaminopimelic acid, glucose, mannose, xylose, ribose and rhamnose in the whole-cell hydrolysate, MK-10(H4), MK-10(H6) and MK-10(H8) as major menaquinones, and iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0 as major cellular fatty acids. blast analysis of 16S rRNA gene sequences revealed that the strain showed highest similarity with Micromonospora palomenae NEAU-CX1T (98.97 %) and Micromonospora coxensis 2-30-b(28)T (98.97 %). Based on phylogenetic tree analysis of the 16S rRNA gene sequence, the strain formed a cluster with M. palomenae NEAU-CX1T, Micromonospora halophytica DSM 43171T, M. coxensis 2-30-b(28)T and Micromonospora purpureochromogenes DSM 43821T. On the basis of phenotypic differences and DNA-DNA relatedness evidence, strain CH3-3T could be clearly distinguished from the closely related species of the genus Micromonospora and represents a novel species of the genus Micromonospora for which the name Micromonospora sediminis sp. nov. is proposed. The type strain is CH3-3T (=JCM 18523T=PCU 350T=TISTR 2396T).

Study of Electricity Utilization in Beach Resort Hotel, Chonburi Province for Energy Conservation

This research investigated the standard electricity utilization in beach resort hotels based on a survey of four 4-star beach resort hotels in Chonburi, Thailand conducted in 2014. The main objective of the project was to know proportion of electricity in end-use category. Each hotel has total usable areas of 34,878.50, 24,706.56, 17,533.40 and 10,910.56 m2 with total air-conditioned areas of 17,364.45, 8,327.48, 5368.35 and 8,212.56 m2 respectively. The results showed that the hotel with most usable areas, air-conditioned space and using central air conditioning system has the minimum index value of electricity utilization for air conditioning system by compared with electrical consumption for air conditioning system per air-conditioned area equal to 72.36 kWh/m2/year. Unlike other three hotels with using split type air conditioning system that have this value equal to 89.92, 103.67 and 136.21 kWh/m2/year. Every hotel has the lowest proportion of electricity in lighting system compared with electrical consumption of air conditioning system and electric-mechanical system, which accounted for 5 – 10% of total electricity utilization and the maximum value of lighting density power is lower than 12 W/m2, which is considered the standard of Thailand Energy Conservation Promotion Act. Moreover, the study also found that the hotel with usable area most has annual electricity consumption for electric-mechanical equipment system that most in refrigerators, followed by pumps and other kitchen equipment, respectively which accounted for 53% of total electricity utilization. Finally, the results can also use as guidelines for designing beach resort hotels, Chonburi province for energy conservation, including the use of air conditioning systems appropriately.