Cholylglycine Research Articles

Exercise in rats with liver injury induced by carbon tetrachloride or bile duct obstruction. Blood cholic acid and liver histology.

Tolerance of exercise in rats with liver injury was evaluated by the response of plasma cholylglycine (CG) and by liver histology in two experiments with CCl4 hepatocellular damage and in two other experiments with cholestatic liver injury induced by obstruction of the common bile duct. (1) Rats swimming daily for 1 h while exposed to five successive doses of CCl4 and studied at rest, had a trend (P less than 0.06) to higher CG and a higher plasma SGPT (P less than 0.05) than sedentary CCl4 rats. (2) In rats recovering from four successive doses of CCl4 there were no differences between CG curves before, during, and after exercise, except at 48 h of recovery when exercise elevated CG (P less than 0.05). (3) Sixteen days after ligation of the bile duct CG was high but not affected by exercise, 45 days after ligation exercise resulted in further increase of CG (P less than 0.05). (4) When ligation of the bile duct lasted only 24 h, exercise prior to release of the obstruction did not affect CG. Exercise 8 h and 27 h after release of ligation resulted in an increase in CG (P less than 0.05). Return to pre-exercise CG occurred 30 min after exercise. CG was a useful indicator of liver injury showing a positive correlation with the CCl4 dose and a negative correlation with recovery from CCl4. Exercise had only mildly adverse effect in hepatotoxic and cholestatic liver injury. CG appeared to respond more sensitively to exercise after release of bile duct obstruction than in CCl4 hepatocellular injury.

Evaluation of enzymic and radioimmunoassay methods for measuring serum bile acids

Serum bile acids were measured in control subjects and in 23 patients with cirrhosis or cholestasis using an enzymic method which measures total non-sulphated bile acids and 2 commercially available radioimmunoassay methods which measure cholylglycine (CG) and sulpholithocholylglycine (SLCG) respectively. All 3 methods gave abnormal values in patients with cholestasis. In cirrhosis the CG method gave abnormal values more often than the enzymic method. The SLCG method was much less sensitive. Measurement of CG in 2 postprandial samples per patient detected only one patient with normal fasting but abnormal postprandial levels. Analysis of samples by gas liquid chromatography (GLC) showed that the enzymic and CG methods gave a good estimation of total non-sulphated bile acids and cholic acid respectively. Values determined by the SLCG method were much higher than the equivalent GLC values. The CG method is faster than the enzymic method and is less expensive provided sample are analysed in sufficiently large batches.

Serum disappearance of cholylglycine (CG)

Serum disappearance of cholylglycine (CG)

Serum levels of cholylglycine (CG) are acutely suppressed by oral administration of chenodeoxycholic acid (CDCA)

Serum levels of cholylglycine (CG) are acutely suppressed by oral administration of chenodeoxycholic acid (CDCA)