Abstract It was recently shown that dietary triglycerides of different degree of unsaturation had different effects on 7α-hydroxylation of cholesterol in rats (Bjorkhem, I., Blomstrand, R., Svensson, L., J. Lipid Res. 19, 359 (1978)). It is shown here that lymphatic drainage in general stimulates 7α-hydroxylation of cholesterol about two-fold regardless of presence or absence of triglycerides in the diet or type of triglycerides. Thus the major differences between the effect of different triglycerides are retained after lymphatic drainage. These results, together with previous results, support the contention that at least part of the effects of different dietary triglycerides on 7α-hydroxylation of cholesterol is related to differences in absorption from the intestine. The possibility is discussed that chylomicrons from the lymph have a direct inhibitory effect on 7α-hydroxylation of cholesterol.