HighlightsThis study investigates the use of laser speckle to evaluate chloroplast movement in plant cells.The analysis value of the laser speckle corresponds to chloroplast movement.The run-length matrix analysis that we propose is more sensitive than existing methods.Abstract. Abiotic and biotic stresses change the cytoplasmic streaming in plant cells. In particular, the state of chloroplast movement changes rapidly in response to environmental changes. Thus, detecting chloroplast movement allows us to comprehend the physiological state of plants. This study investigated the applicability of the laser speckle method for evaluating chloroplast movement. Moreover, we propose a new analysis method for sensitively evaluating chloroplast movement. Leaves of Egeria densa were used, and electrical stimulation was applied as recoverable abiotic stress. Laser speckle measurement and light microscopic observation of the same specimen were performed before and after stimulation. The results showed that the laser speckle method can nondestructively evaluate macroscopic and collective chloroplast movement. The proposed method was more sensitive to chloroplast movement than existing methods. Keywords: Chloroplast, Image processing, Laser speckle, Textual analysis.