Changes Of Chlorophyll Fluorescence Yield Research Articles

Picosecond time-resolved study of MgCl 2-induced chlorophyll fluorescence yield changes from chloroplasts

The MgCl 2-induced chlorophyll fluorescence yield changes in broken chloroplasts, suspended in a cation-free medium, treated with 3,-(3′,4′-dichlorophenyl)-1,1-dimethylurea and pre-illuminated, has been investigated on a picosecond time scale. Chloroplasts in the low fluorescing state showed a fluorescence decay law of the form exp −At 1 2 , where A was found to be 0.052 ps −1 2 , and may be attributed to the rate of spillover from Photosystem II to Photosystem I. Addition of 10 mM MgCl 2 produced a 50% increase in the steady-state fluorescence quantum yield and caused a marked decrease in the decay rate. The fluorescence decay law was found to be predominantly exponential with a 1/e lifetime of 1.6 ns. These results support the hypothesis that cation-induced changes in the fluorescence yield of chlorophyll are related to the variations in the rate of energy transfer from Photosystem II to Photosystem I, rather than to changes in the partitioning of absorbed quanta between the two systems.