Dechlorination Research Articles

Enhanced thyroxine removal from micro-polluted drinking water resources in a bio-electrochemical reactor amended with TiO2@GAC particles: Efficiency, mechanism and energy consumption

A three-dimensional bioelectrochemical system (3D-BES) with both electrocatalytic and biodegradation functions was designed and developed to enhance iodine-containing hormone removal from micro-polluted oligotrophic drinking water sources and to reduce energy consumption. Thyroxine (T4) removal efficiency was 99.0% in the 3D-BES amendment with TiO2@GAC as the particle electrodes, which was 20.5% higher than the total efficiency of single biodegradation (28.7%) plus electrochemical decomposition (49.8%). The high T4 removal efficiency was a result of biochemical synergistic degradation, enhancement of electron transfer and utilization, enrichment of functional microorganisms, and the expression of dehalogenation functional genes. The electron transfer was increased by 1.63 times in 3D-BES compared to the 2D-BES, which contributed to: (i) ∼17.8% enhancement of dehalogenation, (ii) 2.35 times enhancement of the attenuation rate, and (iii) 60% reduction in energy consumption. Moreover, the aggregation of microorganisms and the hydrophobic T4 onto TiO2@GAC shortened the transfer distance of matter and energy, which induced the degradation steps to be shortened and the toxic decay to be accelerated from T4 and its metabolites. These comprehensive functions also enhanced the 31.8% ATPase activity, 7.3% abundance of the functional reductive dehalogenation genera, and 52.3% dehalogenation genes expression for Pseudomonas, Ancylobacter, and Dehalogenimonas, which contributed to an increase in T4 removal. This work provides an environmental-friendly biochemical synergistic method for the detoxification of T4 polluted water.

Short-chain fatty acids facilitated long-term dechlorination of PCBs in Taihu Lake sediment microcosms: Evidence from PCB congener and microbial community analyses

Microbial reductive dechlorination hosts great promise as an in situ bioremediation strategy for polychlorinated biphenyls (PCBs) contamination. However, the slow dechlorination in sediments limits natural attenuation. Short-chain fatty acids, as preferred carbon sources and electron donors for dechlorinating microorganisms, might stimulate PCB dechlorination. Herein, two sets of short-chain fatty acids, sole acetate and a fatty acid mixture (acetate, propionate, and butyrate), were amended periodically into Taihu Lake (China) sediment microcosms containing nine PCB congeners (PCB5, 12, 64, 71, 105, 114, 149, 153, and 170) after 24 weeks of incubation. Short-chain fatty acids facilitated the long-term PCB dechlorination and the promoting effect of the fatty acid mixture compared favorably with that of sole acetate. By the end of 108 weeks, the total PCB mass concentrations in acetate amended and fatty acid mixture amended microcosms significantly declined by 7.6% and 10.3% compared with non-amended microcosms (P < 0.05), respectively. Short-chain fatty acids selectively favored the removal of flanked meta and single-flanked para chlorines. Notably, a rare ortho dechlorination pathway, PCB25 (24-3-CB) to PCB13 (3-4-CB), was enhanced. Supplementary fatty acids significantly increased reductive dehalogenases (RDase) gene pcbA5 instead of improving the growth of Dehalococcoides. These findings highlight the merits of low cost short-chain fatty acids on in situ biostimulation in treating PCBs contamination.

Reductive dechlorination of trichloroethene by sulfided microscale zero-valent iron in fresh and saline groundwater: Reactivity, pathways, and selectivity

S/mZVI is a promising material for groundwater remediation due to its excellent properties. However, the reactivity and electron selectivity toward target contaminant are critical. Thus, this study investigated the effect of complex groundwater chemistries (Milli-Q water, fresh groundwater and saline groundwater) on the reactivity of S/mZVI toward trichloroethylene (TCE), dechlorination pathway, hydrogen evolution kinetic, electron efficiency and aging behaviors. Results showed that sulfidation appreciably increased the reactivity and electron selectivity. The major degradation product of TCE dechlorination by S/mZVI was acetylene, which was consistent with TCE dechlorination by β-elimination. Moreover, reductive β-elimination was still the dominant dechlorination pathway for the application of S/mZVI in three groundwater conditions. However, the rates and the quantities of major products from TCE degradation varied significantly. S/mZVI in saline groundwater can maintain the reactivity towardTCE due to the protection of Fe0 by Fe3O4 deposited on the surface. Thus, the higher TCE removal efficiency and less hydrogen accumulation resulted in the greatest electron efficiency (4.3–79.2%). Overall, S/mZVI was more effective for the application in saline groundwater. This study proved insight into the comprehensive evaluation and implications for the application of S/mZVI based technologies in saline contaminated groundwater.

Assessment of Biological Treatment for Ammonia Removal from Water Resources, Egypt

Egypt is depending entirely on the Nile water. The Rosetta branch of the river Nile serves as a vital freshwater source for domestic, agricultural, industrial, fisheries, and recreational purposes in several western Delta governorates of Egypt, with a daily flow averaging 21,500,000 m3/day. The branch faces significant challenges due to escalating ammonia concentrations stemming from agricultural drains along the river, industrial effluents from industrial activities, and fish farming cages. One particular issue arises during low-demand periods when the flow in the Rosetta branch diminishes. Annually, Egyptian authorities close water flows in a series of channels to facilitate maintenance, resulting in reduced water levels. This winter closure has impacted Kafr El Sheikh and El Beheira, located in northern Egypt, as the drainage of industrial wastes during this period has led to a high pollution load of ammonia and other pollutants. The elevated ammonium content has hindered coagulation, encouraged algal growth, and impeded the chlorine breakpoint during water chlorination processes. Laboratory experiments were conducted to investigate the ability of blue-green algae species (Anabaena sp., Scyndesmos sp., Chlorella sp.) and bacteria (Lactobacillus sp.) to uptake ammonia. This study revealed that single algal species achieved a maximum removal percentage of 30% for ammonia concentrations ranging from 5 to 7.5 mg/l, and mixed algal species attained removal percentages between 6% and 12% at constant retention times of 15 to 60 minutes. For single algal species, retention times exceeding 1 hour (2 and 5 hours) resulted in removal percentages ranging from 85% to 100%. For mixed algal species, removal percentages between 9% and 20% were observed for various retention times ranging from 15 minutes to 1 hour. When comparing the use of algal species mixtures, removal percentages of up to 30-50% were achieved. Additionally, the study found that the application of bacterial species (Lactobacillus sp.) to raw water samples with an average ammonia concentration of 7.8 mg/l resulted in a maximum removal percentage of 100% when using a bacterial content of 100-200 ml per 1-liter raw water sample volume, with varied retention times up to 2 hours.

Formation and estimated toxicity of trihalomethanes and haloacetic acids from chlorination of nonylphenol-containing water: Effect of chlorine and bromide concentration, contact time, and pH

The presence of nonylphenol (NP), an endocrine-disrupting chemical (EDC), in water sources can form toxic chlorination disinfection by-products (DBPs) during the water disinfection process using chlorine. This study investigated the formation of trihalomethanes-4 (THM4) and haloacetic acids-5 (HAA5) as regulated DBPs during the chlorination process of NP-containing water. The effects of chlorine dose, bromide concentration, pH, chlorine contact time, and the presence of NP in real natural water on THM4 and HAA5 formation were investigated. The increase of chlorine dose increased all of the THM4 and HAA5 formation especially chlorinated DBPs (Cl-DBPs), including trichloromethane (TCM) (0.140 %) and dichloroacetic acid (DCAA) (0.722 %) when the chlorine concentration was 5 times higher than NP. However, the increase of bromide concentration increased the formation of brominated DBPs (Br-DBPs), reaching concentration yields of 1.615 % for tribromomethane (TBM) and 0.421 % for dibromoacetic acid (DBAA), but decreased the chlorinated (Cl-DBPs formation). Similarly, the increase of chlorine contact time and pH increased the DBPs formation significantly especially Br-DBPs. The TBM and DBAA concentration yields reached 1.615 % and 0.676 %, respectively, when chlorinated at pH 9 and reached 0.653 % and 0.751 %, respectively, when chlorinated for 168 h. The addition of NP in natural water also raised the hydrophilicity of dissolved organic matter (DOM), indicated by the decrease of specific ultraviolet absorbance (SUVA) value, which enhanced the formation of certain species including THM4 and HAA5. Based on toxicity calculation, the regulated DBPs formed were cytotoxic rather than genotoxic.

Upgrading of conventional water treatment plant by nanofiltration for enhanced organic matter removal

Natural organic matter (NOM) is responsible for a variety of problems in water, including taste, odor, and color. If NOM is present in treated water, it can also promote microbial growth in water distribution systems. Another problem related with NOM is that it combines with chemicals such as chlorine in water resulting in the formation of disinfection by-products (DBPs). With the DBP regulations becoming more stringent, several advanced treatment methods started to be applied to enhance NOM removal. Pilot-scale advanced treatment tests employing tight nanofiltration (T-NF) membrane with high ion rejection capacity and loose nanofiltration (L-NF) membrane with low ion rejection capacity were conducted in this study in order to remove total organic carbon (TOC) having an average concentration of 2 mg/L at the sand filter effluent of a full-scale drinking water treatment plant and to avoid DBP production. The cost of incorporating membrane processes into the treatment process scheme was also determined during the study, in addition to the experimental studies. At constant 6 bar, the average fluxes for the T-NF and L-NF were determined to be 12.7 and 7.6 L /m2 h, respectively. L-NF had a TOC removal efficiency of 96.0%, which is quite similar to the removal efficiency achieved by T-NF (96.3%). Heavy metals, anions, and cations removal efficiency in T-NF membranes were greater than in L-NF, as expected. The removal efficiencies of Cl-, and Fe on T-NF membrane were determined to be 90.3 ± 2.2% and 69.6 ± 5.6, respectively, which are much greater than those achieved in L-NF membrane The total cost of 400,000 m3/d L-NF membrane system upgrade is calculated as 28,901,262 $/y including capital and operation and maintenance (O&M) costs, while in T-NF is calculated as 28,074,282 $/y. The total cost of NF upgrading is estimated to be around 0.191–0.198 $/m3, which is nearly 50% of the cost of the conventional treatment plant. When upgrading a conventional treatment system with this type of membrane system, additional expenses are typically incurred, resulting in a higher total cost; however, the resultant water quality is significantly improved.

Relevance of photocatalytic redox transformations of selected pharmaceuticals in a copper- and iron-rich Mediterranean intermittent river

This work aimed at investigating specific attenuation pathways of pharmaceuticals in copper- and iron-rich Mediterranean intermittent and sunlit rivers by combining lab- and field-scale studies. Poorly photodegradable and biodegradable compounds such as fluconazole, oxazepam and venlafaxine attenuated in two river stretches with short hydraulic residence times (<3 h). This result was assumed to be related to their capacity to interact with photoreactive free Cu2+ and Fe3+ or their associated oxides. Lab-scale photodegradation experiments under simulated solar irradiation revealed the beneficial impact of a mixture Cu2+ and colloidal iron hydroxides at environmental concentrations and at neutral pH on the pharmaceuticals photodegradation kinetic rate constants. These latter were consistent with the in-stream attenuation rate constants of targeted contaminants which ranged from 0.104 to 0.154 h−1. Further identification of phototransformation products by LC-HRMS highlighted reductive transformation pathways including reductive dehalogenation and hydrogenation reactions. Several TPs were found to be stable under irradiation and were detected in field monitoring, accordingly. This was ascribed to the formation of a Cu/Fe composite material under solar irradiation with photocatalytic properties. The role of Cu was to trap the electron in the conduction band of the iron-based photocatalyst, which promoted separation efficiency of electron-hole pairs as well as enhanced photoreduction processes at the expense of oxidation ones. Even though, these mechanisms have been reported in water treatment field for organic micropollutants removal, their significance was demonstrated for the first time in natural settings.

Integrating density functional theory into reductive dechlorination research

AbstractChlorinated organics have been frequently detected in groundwaters, threatening the quality of drinking water supplies worldwide. A promising method for groundwater remediation involves reductive dechlorination (RD), in which chlorine atoms are sequentially removed and substituted by hydrogen, producing less harmful byproducts. In this paper, for the first time, RD research is reviewed in light of the growing incorporation of density functional theory (DFT) as a research tool. DFT has been used to uncover a variety of reaction properties for a range of relevant groundwater pollutants, including 1,2,3‐trichloropropane, hexachlorobenzene, and various dioxins. DFT models have revealed the role of surface interactions in driving the kinetics of catalytically driven RD. Mechanisms involved with biologically mediated RD have also been elucidated with insights gleaned from DFT. Issues and challenges for future research are also discussed.

Contributions of Pharmaceuticals to DBP Formation and Developmental Toxicity in Chlorination of NOM-containing Source Water.

Pharmaceuticals have been considered a priority group of emerging micropollutants in source waters in recent years, while their role in the formation and toxicity of disinfection byproducts (DBPs) during chlorine disinfection remains largely unclear. In this study, the contributions of natural organic matter (NOM) and pharmaceuticals (a mixture of ten representative pharmaceuticals) to the overall DBP formation and toxicity during drinking water chlorination were investigated. By innovatively "normalizing" chlorine exposure and constructing a kinetic model, we were able to differentiate and evaluate the contributions of NOM and pharmaceuticals to the total organic halogen (TOX) formation for source waters that contained different levels of pharmaceuticals. It was found that at a chlorine contact time of 1.0 h, NOM (2 mg/L as C) and pharmaceuticals (total 0.0062-0.31 mg/L as C) contributed 79.8-99.5% and 0.5-20.2%, respectively, of TOX. The toxicity test results showed that the chlorination remarkably increased the toxicity of the pharmaceutical mixture by converting the parent compounds into more toxic pharmaceutical-derived DBPs, and these DBPs might contribute significantly to the overall developmental toxicity of chlorinated waters. This study highlights the non-negligible role of pharmaceuticals in the formation and toxicity of overall DBPs in chlorinated drinking water.

On-line determination of the water cut and chlorine impurities in crude oil using a pulsed beam of fast neutrons

A neutron-based sensor has been developed able to measure the water cut and the chlorine content in crude oil. The sensor is based upon the use of a pulsed fast neutron source. Two different cases were studied. Case ‘‘A’’: chlorine detection with no water present and case ‘‘B’’: water cut measurements with no chlorine present. The minimum detection limits for case A and case B were found to be (62 ± 6) mg/L and (2 ± 2) vol%, respectively.

Tetrachloroethane (TeCA) removal through sequential graphite-mixed metal oxide electrodes in a bioelectrochemical reactor

Electro-bioremediation offers a promising approach for eliminating persistent pollutants from groundwater since allows the stimulation of biological dechlorinating activity, utilizing renewable electricity for process operation and avoiding the injection of chemicals into aquifers. In this study, a two-chamber microbial electrolysis cell has been utilized to achieve both reductive and oxidative degradation of tetrachloroethane (TeCA). By polarizing the graphite granules cathodic chamber at −650 mV vs the standard hydrogen electrode and employing a mixed metal oxide (MMO) counter electrode for oxygen production, the reductive and oxidative environment necessary for TeCA removal has been established. Continuous experiments were conducted using two feeding solutions: an optimized mineral medium for dechlorinating microorganisms, and synthetic groundwater containing sulphate and nitrate anions to investigate potential side reactions. The bioelectrochemical process efficiently reduced TeCA to a mixture of trans-dichloroethylene, vinyl chloride, and ethylene, which were subsequently oxidized in the anodic chamber with removal efficiencies of 37 ± 2%, 100 ± 4%, and 100 ± 5%, respectively. The introduction of synthetic groundwater with nitrate and sulphate stimulated reductions in these ions in the cathodic chamber, leading to a 17% decrease in the reductive dechlorination rate and the appearance of other chlorinated by-products, including cis-dichloroethylene and 1,2-dichloroethane (1,2-DCA), in the cathode effluent. Notably, despite the lower reductive dechlorination rate during synthetic groundwater operation, aerobic dechlorinating microorganisms within the anodic chamber completely removed VC and 1,2-DCA. This study represents the first demonstration of a sequential reductive and oxidative bioelectrochemical process for TeCA mineralization in a synthetic solution simulating contaminated groundwater.

Why do low-cost point-of-use water treatment technologies succeed or fail in combating waterborne diseases in the field? A systematic review

In a world where the consequences of inequalities in access to safe drinking water persist, Household Drinking Water Treatment Technologies (HDWT) remains the most readily available and promising alternative solution to prevent waterborne diseases. While successful cases of combating waterborne diseases with HDWT have been documented, there are also reports of failures in reducing water-related illnesses. Understanding the reasons behind these inconsistent results is crucial. This work aimed to identify and critically discuss barriers, enablers, and technology delivery strategies for HDWT-based interventions to improve drinking water safety. Data from 147 articles covering Solar Disinfection, Chlorination, Flocculation-Disinfection, BioSand Filter, and Ceramic Water Filter were extracted from a total of 11,982 systematically retrieved records. Among the identified factors, 77 barriers and 76 enablers were distributed across different domains: psychosocial (37.7, 47.4%), promotion (22.1, 26.3%), technology (28.6, 10.5%), economic (9.1, 14.5%), and environmental (2.6, 1.3%) for barriers and enablers, respectively. Technology delivery strategies primarily included water education and technology promotion, training for technology usage, provision of HDWT through donation or promotional sale, donation of safe water storage, technical assistance, and the use of a diarrhea control diary. All barriers and enablers affect the initial adoption, regular, and sustained use of HDWT and should be considered when planning and implementing interventions. The health impacts of interventions decreased over time since HDWT adoption. Adequate user training, HDWT donation, and high compliance are important predictors of success for HDWT-based interventions. In general, identifying and exploring the listed enablers can help overcome many of the reported limitations.

Diclofenac Degradation by New AOP System Zero-Valent Aluminium–Acid (ZVAl/H+) Process and its Modifications

Drugs and other pharmaceutical compounds are often used substances around the world. Diclofenac is one of the widely used anti-inflammatory drug for the treatment of acute and chronic pain, rheumatoid and osteoarthritis. In recent years diclofenac was considered as a new emerging environmental contaminant of water and soil ecosystems. In the present work the new AOP system based on the ZVAl/H+ and its modifications was applied to degrade diclofenac water solutions with its initial concentration of 25 mg L−1. The effects of initial pH, dosage of Al0 , addition of hydrogen peroxide, ferrous, ferric and aluminium ions were studied in the dark experiments. In addition, effects of irradiation with sunlight lamp were also investigated. Finally, environmental toxicity studies were conducted according to OECD tests with terrestrial plants. Degradation efficiency was measured by UV-VIS spectroscopy. After all experiments the degree of resulting TOC removal was determined. Concentration of dissolved oxygen in reaction mixture during and after reaction was also monitored. The results showed that all used systems are able efficiently remove diclofenac. The best diclofenac removal reached 98 % efficiency at pH of 3.0 in 4 hours, with Al0 dosage used of 2.0 g L−1 and with addition of 10 mg L−1 of aluminium ions (Al3+). The best TOC removal (83 %) was achieved under experimental condition of ZVAl/H+ with Al0 dosage of 4.0 g L−1 at pH of 3.0 after 4 hours. Negative results with the utilization of radical scavenger for hydroxyl radicals (methanol) led us to conclusion that degradation process in the first steps of reaction proceeds parallel by both two different mechanisms – reductive dehalogenation and hydrogenolysis. Finally, before and after treatment processes toxicity tests according to OECD Guideline 208 were performed. Resulted toxicity values showed that diclofenac solutions in concentration range from 5 to 25 mg L−1 and solutions after treatment processes had only a low toxic effect on terrestrial plants.

Site-specific response of sediment microbial community to supplementation of polyhydroxyalkanoates as biostimulants for PCB reductive dechlorination

The use of biodegradable plastics is constantly raising, increasing the likeliness for these polymers to end up in the environment. Environmental applications foreseeing the intentional release of biodegradable plastics have been also recently proposed, e.g., for polyhydroxyalkanoates (PHAs) acting as slow hydrogen releasing compounds to stimulate microbial reductive dehalogenation processes. However, the effects of their release into the environment on the ecosystems still need to be thoroughly explored. In this work, the use of PHAs to enhance the microbial reductive dechlorination of polychlorobiphenyls (PCBs) and their impact on the metabolic and compositional features of the resident microbial community have been investigated in laboratory microcosms of a polluted marine sediment from Mar Piccolo (Taranto, Italy), and compared with recent findings on a different contaminated marine sediment from Pialassa della Baiona (Ravenna, Italy). A decreased biostimulation efficiency of PHAs on PCBs reductive dechlorination was observed in the sediment from Mar Piccolo, with respect to the sediment from Pialassa della Baiona, suggesting that the sediments' physical-chemical characteristics and/or the biodiversity and composition of its microbial community might play a key role in determining the outcome of this biostimulation strategy. Regardless of the sediment origin, PHAs were found to have a specific and pervasive effect on the sediment microbial community, reducing its biodiversity, defining a newly arranged microbial core of primary degraders and consequently affecting, in a site-specific way, the abundance of subdominant bacteria, possibly cross-feeders. Such potential to dramatically change the structure of autochthonous microbial communities should be carefully considered, since it might have secondary effects, e.g., on the natural biogeochemical cycles.

Bioelectrochemical system accelerates reductive dechlorination through extracellular electron transfer networks

Bioelectrochemical system is considered as a promising approach for enhanced bio-dechlorination. However, the mechanism of extracellular electron transfer in the dechlorinating consortium is still a controversial issue. In this study, bioelectrochemical systems were established with cathode potential settings at −0.30 V (vs. SHE) for trichloroethylene reduction. The average dechlorination rate (102.0 μM Cl·d−1) of biocathode was 1.36 times higher than that of open circuit (74.7 μM Cl·d−1). Electrochemical characterization via cyclic voltammetry illustrated that electrostimulation promoted electrochemical activity for redox reactions. Moreover, bacterial community structure analyses indicated electrical stimulation facilitated the enrichment of electroactive and dechlorinating populations on cathode. Metagenomic and quantitative polymerase chain reaction (qPCR) analyses revealed that direct electron transfer (via electrically conductive pili, multi-heme c-type cytochromes) between Axonexus and Desulfovibrio/cathode and indirect electron transfer (via riboflavin) for Dehalococcoides enhanced dechlorination process in BES. Overall, this study verifies the effectiveness of electrostimulated bio-dechlorination and provides novel insights into the mechanisms of dechlorination process enhancement in bioelectrochemical systems through electron transfer networks.

Insight into the Mechanism Underlying Dehalococcoides mccartyi Strain CBDB1-Mediated B12-Dependent Aromatic Reductive Dehalogenation.

Anaerobic bacteria transform aromatic halides through reductive dehalogenation. This dehalorespiration is catalyzed by the supernucleophilic coenzyme vitamin B12, cob(I)alamin, in reductive dehalogenases. So far, the underlying inner-sphere electron transfer (ET) mechanism has been discussed controversially. In the present study, all 36 chloro-, bromo-, and fluorobenzenes and full-size cobalamin are analyzed at the quantum chemical density functional theory level with respect to a wide range of theoretically possible inner-sphere ET mechanisms. The calculated reaction free energies within the framework of CoI···X (X = F, Cl, and Br) attack rule out most of the inner-sphere pathways. The only route with feasible energetics is a proton-coupled two-ET mechanism that involves a B12 side-chain tyrosine (modeled by phenol) as a proton donor. For 12 chlorobenzenes and 9 bromobenzenes with experimental data from Dehalococcoides mccartyi strain CBDB1, the newly proposed PC-TET mechanism successfully discriminates 16 of 17 active from 4 inactive substrates and correctly predicts the observed regiospecificity to 100%. Moreover, fluorobenzenes are predicted to be recalcitrant in agreement with experimental findings. Conceptually, based on the Bell-Evans-Polanyi principle, the computational approach provides novel mechanistic insights and may serve as a tool for predicting the energetic feasibility of reductive aromatic dehalogenation.

Unlocking the potential of resuscitation-promoting factor for enhancing anaerobic microbial dechlorination of polychlorinated biphenyls

Microbial dechlorination of polychlorinated biphenyls (PCBs) is limited by the slow growth rate and low activity of dechlorinators. Resuscitation promoting factor (Rpf) of Micrococcus luteus, has been demonstrated to accelerate the enrichment of highly active PCB-dechlorinating cultures. However, it remains unclear whether the addition of Rpf can further improve the dechlorination performance of anaerobic dechlorination cultures. In this study, the effect of Rpf on the performance of TG4, an enriched PCB-dechlorinating culture obtained by Rpf amendment, for reductive dechlorination of four typical PCB congeners (PCBs 101, 118, 138, 180) was evaluated. The results indicated that Rpf significantly enhanced the dechlorination of the four PCB congeners, with residual mole percentages of PCBs 101, 118, 138 and 180 in Rpf-amended cultures being 16.2–29.31 %, 13.3–20.1 %, 11.9–14.4 % and 9.4–17.3 % lower than those in the corresponding cultures without Rpf amendment after 18 days of incubation. Different models were identified as appropriate for elucidating the dechlorination kinetics of distinct PCB congeners, and it was observed that the dechlorination rate constant is significantly influenced by the PCB concentration. The supplementing Rpf did not obviously change dechlorination metabolites, and the removal of chlorines occurred mainly at para- and meta- positions. Analysis of microbial community and functional gene abundance suggested that Rpf-amended cultures exhibited a significant enrichment of Dehalococcoides, Dehalogenimonas and Desulfitobacterium, as well as non-dechlorinators belonging to Desulfobacterota and Bacteroidetes. These findings highlight the potential of Rpf as an effective additive for enhancing PCB dechlorination, providing new insights into the survival of functional microorganisms involved in anaerobic reductive dechlorination.

Degradation of 1,2-dichloroethane in real polluted groundwater by using enriched bacterial consortia in aerobic and anaerobic laboratory-scale conditions

The aim of this work was to gain insights about the feasibility of chlorinated solvents removal through biostimulated and bioaugmented biological processes in laboratory-scale permeable reactive barriers (PRBs) under anaerobic and aerobic conditions. The experimental plant consisted of two Plexiglas cylindrical columns filled with silica sand and fed with real groundwater contaminated by chlorinated solvents (mainly 1,2-dichloroethane, 1,2-DCA, at a concentration of 20 mg l−1). Column A simulated a PRB containing poly-β-hydroxybutyrate (PHB) powder as electron donor and worked under anaerobic conditions; in Column B an inlet air flow rate ensured aerobic conditions. Both columns were inoculated with dechlorinating bacterial consortia obtained by enrichment cultures from the same contaminated groundwater. Results from Column A showed that PHB can be fermented and used as a slow-releasing carbon source for sustaining reductive dechlorination, as revealed by acetate production up to 267 mg l−1 and 100% 1,2-DCA removal. The microbial community detected in Column A at the end of the experimental period was mainly enriched in sulfate reducing bacteria that could act as both fermenting and dechlorinating agents. Column B showed a slight lower 1,2-DCA removal efficiency (98%) likely related to the establishment of aerobic (co)metabolic processes.

The effects of arsenic on dechlorination of trichloroethene by consortium DH: Microbial response and resistance

Inorganic arsenic and organochlorines are frequently co-occurring contaminants in anoxic groundwater environments, and the bioremediation of their composite pollution has long been a rigorous predicament. Currently, the dechlorination behaviors and stress responses of microbial dechlorination consortia to arsenic are not yet fully understood. This study assessed the reductive dechlorination performance of a Dehalococcoides-bearing microcosm DH under gradient concentrations of arsenate [As(V)] or arsenite [As(III)] and investigated the response patterns of different functional microorganisms. Our results demonstrated that although the dechlorination rates declined with increasing arsenic concentrations in both As(III/V) scenarios, the inhibitory impact was more pronounced in As(III)-amended groups compared to As(V)-amended groups. Moreover, the vinyl chloride (VC)-to-ethene step was more susceptible to arsenic exposure compared to the trichloroethene (TCE)-to-dichloroethane (DCE) step, while high levels of arsenic exposure [e.g. As(III) > 75 μM] can induce significant accumulation of VC. Functional gene variations and microbial community analyses revealed that As(III/V) affected reductive dechlorination by directly inhibiting organohalide-respiring bacteria (OHRB) and indirectly inhibiting synergistic populations such as acetogens. Metagenomic results indicated that arsenic metabolic and efflux mechanisms were identical among different Dhc strains, and variations in arsenic uptake pathways were possibly responsible for their differential responses to arsenic exposures. By comparison, fermentative bacteria showed high potential for arsenic resistance due to their inherent advantages in arsenic detoxification and efflux mechanisms. Collectively, our findings expanded the understanding of the response patterns of different functional populations to arsenic stress in the dechlorinating consortium and provided insights into modifying bioremediation strategies at co-contaminated sites for furtherance.

Community‐driven science and science education: Living in and navigating the edges of equity, justice, and science learning

Community‐driven science and science education: Living in and navigating the edges of equity, justice, and science learning