Abstract The purpose of this study was to determine how cold-induced stress affects the maturing of mouse bone marrow-derived dendritic cells (BMDCs) and to see the dynamics of cytokines production associated with T helper subtypes. Quantitative real time PCR and ELISA were used to measure gene expression and production of cytokines in co-culture of BMDC and Naïve CD4+ T cells. Culturing BMDC in vitro stimulated with LPS in the presence of norepinephrine (NE) or Feroterol, β-AR agonist resulted in a marked increase (75 pg/ml) of IL-10 compared to that of stressed mice. Treatment with β-AR antagonist, ICI 118,551 resulted in lowering production of IL-10 in BMDC of nonstressed (control) mice. In contrast, IL-12 production in stressed group was significantly low (132 vs 239 pg/ml) compared to that of control mice. Naïve T cells co-cultured with BMDC pretreated with NE or Feroterol showed decreased IL-12 production compared to controls. In contrast IL-4 production was increased with pretreatment of NE or Feroterol (64 vs 28 pg/ml) compared to control group. Increased IL-4 production in the genital tracks of mice compared to control (114 vs 0 pg/ml) was observed. The relative expression of GTAT 3 in T cells co-cultured with BMDC of stressed mice was high (2.8 vs 0.49) compared to control mice. The same gene expression trend of IL-4 in stressed mice group (1.8 versus 0.12) compared to control group was observed. In control mice, T-bet expression was (1.78 vs 1.0) and interferon gamma (2.12 versus 1.0) compared to that of stressed mice group. Our results demonstrate that stress influences the activation and differentiation of DCs stimulated by LPS shifting the Th1/Th2 ratio and impairing the production of Th1 protective cytokines during Chlamydia muridarum infection.