Chitosan Succinate Research Articles

Preparation and Characterization of Novel Polyelectrolyte Liposomes Using Chitosan Succinate Layered over Chitosomes: A Potential Strategy for Colon Cancer Treatment.

Chitosan succinate is distinguished by its ability to shield the loaded drug from the acidic environment, localize and keep the drug at the colon site, and release the drug over an extended time at basic pH. The current study attempts to develop polyelectrolyte liposomes (PEL), using chitosan and chitosan succinate (CSSC), as a carrier for liposomal-assisted colon target delivery of 5 fluorouracil (5FU). The central composite design was used to obtain an optimized formulation of 5FU-chitosomes. The chitosan-coated liposomes (chitosomes) were prepared by thin lipid film hydration technique. After that, the optimized formulation was coated with CSSC, which has several carboxylic (COOH) groups that produce an anionic charge that interacts with the cation NH2 in chitosan. The prepared 5FU-chitosomes formulations were evaluated for entrapment efficiency % (EE%), particle size, and in vitro drug release. The optimized 5FU-chitosomes formulation was examined for particle size, zeta potential, in vitro release, and mucoadhesive properties in comparison with the equivalent 5FU-liposomes and 5FU-PEL. The prepared 5FU-chitosomes exhibited high EE%, small particle size, low polydispersity index, and prolonged drug release. PEL significantly limited the drug release at acidic pH due to the deprotonation of carboxylate ions in CSSC, which resulted in strong repulsive forces, significant swelling, and prolonged drug release. According to a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay, PEL treatment significantly decreased the viability of HT-29 cells. When compared to 5FU-liposome and 5FU-chitosome, the in vivo pharmacokinetics characteristics of 5FU-PEL significantly (p < 0.05) improved. The findings show that PEL enhances 5FU permeability, which permits high drug concentrations to enter cells and inhibits the growth of colon cancer cells. Based on the current research, PEL may be used as a liposomal-assisted colon-specific delivery.

Improving the technology for obtaining of low molecular weight chitosan under conditions of controlled enzymatic cleavage

Relevance. Annotation. Experimental data on improving the production technology of regulated enzymatic cleavage of chitosan to obtain its low-molecular derivatives are presented. Experimental studies were carried out in the conditions of the Department of Disease Diagnostics, Therapy, Obstetrics and Animal Reproduction of the Moscow State Academy of Veterinary Medicine and Biotechnology — MVA named after K.I. Skryabin and on the basis of the All-Russian Research and Technological Institute of the Biological Industry.Methods. Acid-soluble chitosan from king crab shell (MM 700 kDa, SDA 85%), chitosan succinate (MM 330 kDa, SZ 75.2%), low-molecular food chitosan (hydrochloride) (MM 50 kDa) were used as raw materials for obtaining new modifications of chitosan, gel chitosan (2% solution in 2% acetic acid) manufactured by Bioprogress LLC.Results. As a result of the experiment, the technological parameters of obtaining low-molecular chitosan by enzymatic hydrolysis were selected. The greatest decrease in the dynamic viscosity of chitosan was reliably observed in the pilot-industrial series II (by 29.3 times), and the pilot-industrial series I and III — by 6.9 and 10.6 times, respectively. As a result of enzymatic hydrolysis of chitosan in the pilot series II, it was possible to reduce the molecular weight from 700 to 24 kDa, and in series I and III — to 102 and 66 kDa. Based on the research results, a technological scheme for obtaining low-molecular-weight chitosan derivatives by enzymatic hydrolysis has been developed, tested and proposed for industrial use, technical conditions have been developed.

Composite and Nanocomposite Thin-film Structures Based on Chitosan Succinamide

Aim: Currently, developing composite and nanocomposite materials based on natural polymers is attracting the growing attention of scientists. In particular, chitosan succinate, a modified biopolymer, has good biocompatibility, biodegradability, and electrical conductivity, allowing it to be used as a functional material for creating various electronic devices, including sensors for use in medicine and pharmaceuticals. Composite sensors based on chitosan derivatives have found application for the recognition and determination of enantiomers of tryptophan, tyrosine, naproxen, and propranolol in human urine and blood plasma in tablet forms of drugs without a preliminary active substance. Methods: This article discusses the studies on composite and nanocomposite thin-film structures based on chitosan succinamide obtained using various fillers, such as graphene oxide, singlewalled carbon nanotubes, and carbon adsorbents. Result: The studies used cyclic voltammetry, electrochemical impedance spectroscopy, and atomic force microscopy. The results created field-effect transistors based on the films in question as the transport layer. Conclusion: The mobility of charge carriers was estimated, and the following values were obtained: μ(SCTS) = 0.173cm2 /V·s; μ(SCTS-GO) = 0.509 cm2 /V·s; μ(SCTS-CP) = 0.269 cm2 /V·s; μ(SCTS-CB) = 0.351cm2 /V·s; μ(SCTS-SWCNT) = 0.713 cm2 /V·s.

Chitosan and Chitosan Succinate as Photoprotectors for Papain Immobilized on Their Matrix

Chitosan and Chitosan Succinate as Photoprotectors for Papain Immobilized on Their Matrix

Innovative prospects for the creation of biologically active complexes based on a polysaccharide matrix

The possibility of combining complexly soluble arabinogalactan polysaccharides with chitosan polysaccharides to increase the absorption of biologically active substances and compounds characterized by low bioavailability is being considered. Included in the biologically active biopolymer matrix are chitosan succinate, succinic anhydrite, arabinogalactan and dihydroquercetin, respectively, in quantities, wt. %: 3–5.0; 2.0–4.0; 15.0–20.0 and 3.5 can serve as a targeted carrier for the delivery of diagnostic and therapeutic agents, including enzymes, nucleic acids, vitamins, hormones, etc. to certain cells, in particular, hepatocytes (liver parenchyma cells). The high membranotropic properties of arabinogalactan isolated from larch have been established, which allows its use to increase the absorption in the gastrointestinal tract of other functionally active ingredients with low bioavailability. The mechanism of this action is associated with the formation of a binding complex between arabinogalactan and the delivered agent, which has the ability to interact with the asialoglycoprotein cell receptor. Studies have been carried out on the possibility of combining arabinogalactan polysaccharides with chitosan polysaccharides to obtain a biopolymer matrix, which includes chitosan succinate, succinic anhydride and arabinogalactan containing dihydroquercetin not covalently bound to it. Along with the above properties, the effectiveness of using arabinogalactan is shown using the example of its use to improve the technological and functional characteristics of broiler chicken meat with PSE traits. The addition of arabinogalactan to the recipe composition of minced meat during the production of sausages, in an amount of 0.5% of the main raw material, increased the moisture-binding capacity by 5% while reducing losses during heat treatment by 6%. There was an improvement in peroxide and acid numbers during storage, which ensures inhibition of oxidative processes and prevents spoilage of finished products.

СОРБЦИЯ КАДМИЯ И СВИНЦА ВЕЩЕСТВАМИ, ПЕРСПЕКТИВНЫМИ ДЛЯ СОЗДАНИЯ КОМПОЗИЦИОННЫХ ПРЕПАРАТОВ НА ОСНОВЕ ГЕКСАЦИАНОФЕРРАТОВ

The development of new drugs and feed additives to reduce the accumulation of anthropogenic and natural toxicants (heavy metals, radionuclides, mycotoxins, pesticides, etc.) in the body of farm animals and livestock products remains an urgent task. One of the ways to solve it is to create multifunctional products containing ferrocyanides (hexacyanoferrates) of transition metals as active substances. In medicine and veterinary medicine, enterosorbents based on compounds of this class are used as independent pharmacological agents, and in the form of composite preparations, which are obtained by fixing ferrocyanides on carrier substances of different origin and chemical composition. To study the possibility of using certain substances as carriers in the creation of new composite preparations and to assess their effect on the detoxification properties of ferrocyanides, the sorption efficiency of a number of mineral and organic substances with respect to Cd and Pb was studied. It was found that the background content of Cd and Pb in the tested substances does not exceed the maximum permissible levels of these toxicants in feed, feed additives and veterinary preparations. Under in vitro conditions, the biomass of the fungus Inonotus obliquus (chaga), the preparations ferrocin, bifezh and KhZh-90, zeolite tuff shivyrtuin, smectite dioctahedral, white soot and chitosan succinate have the greatest efficiency of Cd and Pb sorption (with respect to Cd – 50.4-89.0%, Pb – 58.3-95.1%). The combined presence of Cd and Pb reduces the sorption capacity of most of the tested substances by 1.6-63.6%.

Development and Investigation of Hierarchically Structured Thin-Film Nanocomposite Membranes from Polyamide/Chitosan Succinate Embedded with a Metal-Organic Framework (Fe-BTC) for Pervaporation.

Thin-film composite membranes (TFC) obtained by the formation of a selective layer on a porous membrane-substrate via interfacial polymerization (IP) are indispensable for separation procedures in reverse osmosis, nanofiltration, pervaporation, and gas separation. Achieving high selectivity and permeability for TFC membranes is still one of the main challenges in membrane science and technology. This study focuses on the development of thin film nanocomposite (TFN) membranes with a hierarchically structured polyamide (PA)/chitosan succinate (ChS) selective layer embedded with a metal–organic framework of iron 1,3,5-benzenetricarboxylate (Fe-BTC) for the enhanced pervaporation dehydration of isopropanol. The aim of this work was to study the effect of Fe-BTC incorporation into the ChS interlayer and PA selective layer, obtained via IP, on the structure, properties, and performance of pervaporation TFN membranes. The structure and hydrophilicity of the developed TFN membranes were investigated using scanning electron microscopy (SEM) and atomic force microscopy (AFM), along with water contact angle measurements. The developed TFN membranes were studied in the pervaporation dehydration of isopropanol (12–30 wt % water). It was found that incorporation of Fe-BTC into the ChS interlayer yielded the formation of a smoother, more uniform, and defect-free PA ultrathin selective layer via IP, due to the amorpho-crystalline structure of particles serving as the amine storage reservoir and led to an increase in membrane selectivity toward water, and a slight decrease in permeation flux compared to the ChS interlayered TFC membranes. The best pervaporation performance was demonstrated by the TFN membrane with a ChS-Fe-BTC interlayer and the addition of 0.03 wt % Fe-BTC in the PA layer, yielding a permeation flux of 197–826 g·m−2·h−1 and 98.50–99.99 wt % water in the permeate, in the pervaporation separation of isopropanol/water mixtures (12–30 wt % water).

Novel Thin Film Nanocomposite Membranes Based on Chitosan Succinate Modified with Fe-BTC for Enhanced Pervaporation Dehydration of Isopropanol.

The application of environmentally friendly and energy-efficient membrane processes allows improvement the ecological safety and sustainability of industrial production. However, the effective application of membrane processes requires novel high-performance thin film composite (TFC) membranes based on biopolymers to solve environmental problems. In this work for the first time novel thin film nanocomposite (TFN) membranes based on biopolymer chitosan succinate (ChS) modified with the metal organic framework iron 1,3,5-benzenetricarboxylate (Fe-BTC) were developed for enhanced pervaporation dehydration. The formation of a selective layer of TFN membranes on the porous membrane-support was carried out by two methods—dynamic technique and physical adsorption. The effect of the membrane formation method and Fe-BTC content in ChS layer on the structure and physicochemical properties of TFN membranes was investigated. The developed TFN ChS-based membranes were evaluated in the pervaporation dehydration of isopropanol (12–30 wt.% water). It was found that TFN ChS-Fe-BTC membranes prepared by two methods demonstrated improved permeation flux compared to the reference TFC ChS membrane. The best transport properties in pervaporation dehydration of isopropanol (12–30 wt.% water) were possessed by TFN membranes with 40 wt.% Fe-BTC prepared by dynamic technique (permeation flux 99–499 g m−2 h−1 and 99.99% water in permeate) and TFN membranes with 5 wt.% Fe-BTC developed by physical adsorption (permeation flux 180–701 g m−2 h−1 and 99.99% water in permeate).

Содержание липидов в яйце при добавлении в рацион кур-несушек кислото- и водорастворимого хитозана

The studies were carried out on laying hens of the Ptichnaya cross at the age of 19 weeks. with an initial live weight of 1,67 kg for 1,5 months, who were given a complete mixed feed (recipe No. KK 1-58-59576, (OJSC Istra-Hleboprodukt) at 135 g/bird/day. The hens were divided into 4 groups: the 1st was the control group received BR without additives, the 2nd and 3rd received BR + acid-soluble chitosan (ASC) at 10 and 20 mg/kg of live weight, respectively, the 4th received BR + 20 mg kg/l of live weight of water-soluble chitosan succinate (WSCC). Inclusion in the diet of laying hens of both forms had practically no effect on the content of total lipids in the yolk. In the eggs of group 2 hens, the content of total lipids in relation to the control at the age of 30 weeks was higher by 10,3 %, at 52 weeks it was lower by 3 %, and at 65 weeks it was lower by 0,76 %. In the eggs of hens of the 3rd group, the content of lipids at the age of 30 weeks was higher than in the control samples of eggs by 12,8 %, at 52 weeks by 1,2 %, and 65 weeks no differences were found. WSCC had no effect on the content of phospholipids, and ASC increased their concentration (depending on the dose). At a lower dose, an increase in the level of phospholipids in the yolk in the 1,8 times. In the eggs of hens of the 2nd group, the concentration of phospholipids was higher than in the control ones at the age of 30 weeks. by 85 %, at 52 weeks by 51,6 %, and at 65 weeks by 50%. In the eggs of hens of group 3, the concentration of phospholipids was higher compared to the data of eggs of hens of the control group at the age of 30 weeks. by 36,5 %, at 52 weeks. by 8 % and 65 weeks – by 9,6 %. The introduction of ASC at a dose of 10 mg/kg of live weight into the diet of chickens was accompanied by a decrease in cholesterol content by 1,5 times, and WSCC at a dose of 20 mg – by 2 times. The content of polyunsaturated fatty acids in the eggs of laying hens between the control and experimental groups did not have significant differences and their values are within the established norms. When ASC was included in the diet of laying hens at a dosage of 20 mg/kg of live weight, a 15 % decrease in the amount of polyunsaturated fatty acids was observed, with an increase in the content of fatty acids of the ω-3 family in the whole egg by 24 %.

Cooked sausage enriched with essential nutrients for the gastrointestinal diet

Introduction. People with gastrointestinal disorders should have a sparing diet with a balanced chemical and amino acid composition including all essential components. Based on formulations of meat products, we identified a number of essential nutritional components that could improve the diet for gastrointestinal pathologies. In this study, we aimed to develop a formulation for cooked sausage enriched with deficient essential nutrients.&#x0D; Study objects and methods. Our study object was cooked sausage. First, we analyzed the diet for people with gastrointestinal disorders. Then, we formulated a meat-based product (cooked sausage), determined its chemical and amino acid compositions, as well as vitamin and mineral contents, and assessed the balance of amino acids. Finally, we evaluated the biological value and safety of the formulated sausage on laboratory mice.&#x0D; Results and discussion. The chemical and amino acid compositions of a daily gastrointestinal diet in medical institutions revealed a deficiency of some water-soluble vitamins, vitamin A, calcium, magnesium, and iron, as well as an imbalance of amino acids. To replenish the deficiency, we formulated a meat-based product composed of trimmed beef and pork, beef liver, egg mix, food gelatin, chitosan succinate, rice flour, and soy fortifier. The product was classified as a meat and cereal cooked sausage of grade B, in which most amino acids were used for anabolic purposes. Its daily portion of 100 g eliminated the deficiency of potassium and iron, almost completely replenished magnesium, calcium, and vitamin A, as well as reduced the deficiency of dietary fiber by 4.8 g. The cytological studies of the blood of laboratory animals, whose basic diet contained the formulated sausage, proved its high biological value and safety.&#x0D; Conclusion. We found that the formulated meat and cereal sausage can be included in the diet for patients with gastrointestinal diseases and used in medical institutions to eliminate the deficiency of essential nutrients.

Interaction of Bacterial Collagenase with the Matrix of Chitosan Ion-Exchange Fibers, Chitosan, and Chitosan Succinate During Immobilization

Interaction of Bacterial Collagenase with the Matrix of Chitosan Ion-Exchange Fibers, Chitosan, and Chitosan Succinate During Immobilization

Результаты экспериментального исследования гидрогелевого дренажа, содержащего ранибизумаб при антиглаукомных операциях

Purpose. Еxperimental substantiation of the effectiveness of biocompatible biodegradable hydrogels based on hyaluronic acid and chitosan succinate as a carrier of ranibizumab in antiglaucoma operations. Material and methods. Hydrogel drainage (HD) was obtained immediately before surgery. A solution of ranibizumab (0.23 ml) was mixed with a solution of hyaluronic acid dialdehyde (0.5 ml), then a solution of chitosan succinate (0.5 ml) was added. Experimental studies were performed in 12 (12 eyes) healthy rabbits. The first group consisted of 6 eyes – 0.187 ml of ranibizumab per 1 ml of gel. In the control group, HD was used intraoperatively without the addition of ranibizumab (6 eyes). Morphological studies were performed on 7th, 21st, and 42nd days. Results. In experimental studies in vitro and in vivo, it was proved that ranibizumab, administered as a part of 0.1 ml of hydrogel drainage in the antiglaucoma surgery area is released within 3 weeks and suppresses vascularization, scarring of the operating area, and preserves the intrascleral cavity. The optimal concentration of ranibizumab was selected-0.02 ml in 0.1 ml of gel. Conclusion. The safety and effectiveness of the use of hydrogel drainage with ranibizumab based on hyaluronic acid dialdehyde and chitosan succinate in anti-glaucoma operations has been proven. Key words: experimental research, hydrogel drainage, ranibizumab, glaucoma surgery.

Hemostatic Compositions Based on Chitosan Complexes with Calcium Ions

The production of new effective hemostatic materials based on a polymer composition of modified chitosan with a three-dimensional cross-linked structure as a polymer matrix and calcium ions coordinated to it is addressed. The optimum concentration of calcium ions in the chitosan-based composition was established as 10 – 20 mM and ensured an effective in vitro blood coagulation time of 40 sec. Compositions of chitosan succinate and chloride in addition to chitosan in solutions with nicotinic acid were shown to have effective hemostatic activity. Statistical differences in the effects of various calcium salts on the blood coagulation time were not found. In vivo tests showed that a chitosan-based composition was effective for stopping bleeding of any degree of severity, both with external and internal injuries to tissues.

Experience of using stimulating feeding for honey bees "BiHit" in the Moscow region and the Republic of Crimea

Unfavorable environmental factors: pesticides, chemicals, poor nutrition of bee colonies lead to a decrease in the bee's immune defense. Currently, there is a continuous search for biologically active substances that stimulate the vital activity of bees and increase the egg-laying of queens. Analysis of scientific sources data has shown high efficiency for stimulating the viability and increasing the productivity of bees of such biologically active additives as chitosan and its derivatives, probiotic strains of bacteria Bacillus subtilis and Bacillus Lichineformis, as well as sources of vital amino acids. The experience accumulated to date in the use of chitosan and its derivatives in veterinary medicine makes it possible to classify chitosan as an effective adaptogen with multifunctional properties capable of providing the body's resistance to numerous adverse environmental factors and pathogens of various diseases. Due to the excess positive charge, chitosan acquires the properties of a highly active anionite. It binds and firmly holds ions of various heavy metals, radioactive isotopes, toxic elements with their subsequent removal from the body of animals, including bees. Chitosan and its derivatives have high antibacterial, sorption, fungicidal, antiviral activity, being a natural biocompatible product that is biodegradable to CO2 and H2O and does not pollute the environment. To stimulate the vital activity of bees and increase the egg-laying of queens, stimulating protein supplements are needed to cover the lack of vital amino acids, probiotic preparations that improve the microbiocenosis of the intestines of bees, sugar syrup. Earlier, at the preliminary stage of research, we developed a recipe for stimulating feeding for bees "BiChit", which includes low molecular weight chitosan, chitosan succinate, dry bacterial mass of Bacillus subtilis VKM B-2716D and Bacillus licheniformis VKM B-2717D, lactose-free edible glucose hydrolyzate, lactose-free whey. In this work, the influence of the multifunctional stimulating feeding "BiHit" on the economically useful traits of bee colonies based on private apiaries in the Moscow region and the Republic of Crimea has been studied. Studies have shown a positive effect of feeding on family strength, brood and honey production.

Method Development for Ficin Entrapment into Gels Based on Food-Grade Chitosan and Chitosan Succinate

Ficin was immobilized by entrapment into gels based on food-grade chitosan and chitosan succinate. The optimum ratio of protein content (mg/g of carrier), total activity (in U/mL of solution), and specific activity (in U/mg of protein) was achieved when ficin was immobilized by entrapment into the gels (based on food-grade chitosan and chitosan succinate) using glycine buffer at pH 9.0 and 9.5, respectively. The proposed technology is accessible for Russian laboratories and is promising for further investigation of the immobilized ficin preparations in medicine and the pharmaceutical industry because of the combination of relatively inexpensive components.

Chitosan succinate and food-grade chitosan for the creation of tissue-engineering structures by the laser method

Laser radiation makes it possible to form a composite material with laser radiation, which is intended for use as cellular- and tissue-engineering structures. It is important to obtain a uniform distribution of components throughout the volume of such material. Intermolecular interactions between chitosan and single-walled carbon nanotubes (SWCNTs) affect the structure of the composite. The results of IR spectroscopy and nonlinear optical studies have shown that the effect of laser radiation has different effects on the two types of chitosan, which was clearly demonstrated by SEM studies. Upon detailed examination, agglomerates in the composite material are clearly visible. Chitosan succinate less prevents the formation of agglomerates from SWCNTs, as a result of which a high agglomeration of nanotubes occurs among themselves in the composite material. The corresponding formations are distinguishable in the SEM images.

Morphological and biochemical parameters of cow blood when using chitosan preparations

The study of the effect of chitosan succinate use on the hematological indicators of dairy cows is relevant. It has been established that there was a clear tendency to increase in the number of white blood cells by 10.12% in animals of group 3 (P &lt; 0.1) on the 10th day of the experiment in cows receiving low-molecular-weight chitosan succinate, in the second group the increase in this indicator by 2.94% was unreliable. On the 20th day of the experiment, an intensive increase in the number of white blood cells was observed in animals of both experimental groups by 14.63–17.38% (P≤0.01), respectively, for the experimental groups of cows. On the 10th day on the background of the use of low-molecular-weight chitosan (group 3), a significant decrease in the number of red blood cells was found, compared with the background, by 8.34% (P≤0.05). On the 20th day, the decrease in the number of red blood cells was already significant in both experimental groups, and their level was 6.09±0.33...6.38±0.20·109/l (P≤0.05), respectively. On the 60th day of the study, this trend was maintained only in group 3, and the concentration of red blood cells was lower by 12.64 (P &lt; 0.05) compared to the initial values. By the 60th day, there was an increase in the level of hemoglobin index up to 126.12±9.10 g/l.

Адьювантная активность хитозана при конструировании вакцины против инфекционного ринотрахеита крупного рогатого скота

The paper presents the results of studying the immunostimulating properties of chitosan in the design of a vaccine against infectious rhinotracheitis in cattle. The optimal concentration of chitosan succinate as an adjuvant of the vaccine against infectious bovine rhinotracheitis was established at 0.5%. At the same time, the optimal interval between immunizations was 21 days. The work shows that the use of chitosan succinate as an adjuvant can significantly increase the level of post-vaccination immunity by 5.33 log2 in rabbits, which indicates the high adjuvant properties of chitosan.

ESTIMATING THE STABILITY OF METAL–LIGAND BONDING IN CARBOXYL-CONTAINING POLYMER COMPLEXES BY IR SPECTROSCOPY

The stability of metal–ligand (carboxyl-containing polymer (CCP)) bonding in transition metal complexes is estimated and juxtaposed using Δν(COO) values. The Δν(COO) value is determined from the difference between stretching vibrations of the carboxylate ion in the IR spectra. It is shown that Δν(COO) values for transition metal complexes with ethylenediaminetetraacetic acid (EDTA) increase in the order that directly depends on the order of their stability in the series Fe3+ > Cr3+ > Cu2+ > Ni2+ > Co2+ > Mn2+. The established relationship between the Δν(COO) value and the stability of metal complexes is used to determine the series of metal affinity for carboxyl-containing biopolymers such as citrus pectin (CP) and chitosan succinate (CS). The obtained data indicate that the affinity for metal corresponds to series Cr3+ > Cu2+ > Co2+ > Mn2+ > Ni2+ for citrus pectin (CP) and to series Cu2+ > Cr3+ ≈ Ni2+ ≈ Co2+ > Mn2+ for chitosan succinate (CS).

БІОТЕХНОЛОГІЧНІ ОСНОВИ ТЕХНОЛОГІЇ КОНСТРУЮВАННЯ «GREEN ARTICLE» ДЛЯ УПРАВЛІННЯ ХАРАКТЕРИСТИКАМИ БІОКЕРАМІЧНОГО ЗАХИСНОГО ШАРУ ШКАРАЛУПИ ЯЄЦЬ КУРЕЙ В ТЕХНОЛОГІЧНОМУ ПРОЦЕСІ ІНКУБАЦІЇ

Авторами теоретично обґрунтована та практично доведена ефективність застосування розробленого способу передінкубаційної обробки яєць з використанням біоміметичної технології «штучна кутикула» «ARTICLE» («ARTIficial cutiCLE») «GREEN ARTICLE» для поліпшення структурних і фізіологічних характеристик шкаралупи як біокерамічного захисного шару інкубаційних яєць, котра полягає в утворенні на поверхні яйця захисної плівки з суміші штучних і природних матеріалів, що регулює рівень газопроникності шкаралупи пташиного яйця. Захисна плівка являє собою полікомпонентне покриття для відновлення та посилення бар’єрних властивостей біокерамічних структур шкаралупи і шкаралупних мембран, якому притаманна біоцидна (антибактеріальна та антивірусна) активність, а також здатність оптимізувати газообмін ембріонів протягом інкубації та поліпшувати процеси обміну речовин ембріона і якість молодняка птиці. Метою нашої роботи була розробка технології передінкубаційної обробки курячих яєць «GREEN ARTICLE» з використанням захисних нанокомпозитних покриттів «Штучна кутикула», що були створені за біоміметичним принципом, для зниження контамінації патогенною мікрофлорою та підвищення виводимості яєць. Для проведення досліду було сформовано три партії яєць, котрі були отримані від курей-несучок Легорн білий, по 540 штук в кожній групі. В кожній партії був контроль і дослідна група. Інкубацію проводили в інкубаторі «Універсал» протягом 21 доби згідно методики. Першу групу яєць перед закладкою на інкубацію обробляли кислото розчинним хітозаном, другу – водорозчинним хітозаном, третю – водорозчинним хітозаном сукцинат. Рахували відсоток браку інкубації, вивід та виводимість курчат. Перед закладкою на інкубацію, а також на сьому та вісімнадцяту добу інкубації, перед перенесенням яєць у вивідну шафу, брали змиви з поверхні шкаралупи і досліджували їх на наявність мікробної контамінації. Доведено, що використання для передінкубаційної дезінфекції курячих яєць композиції на основі кислоторозчинного хітозана з додаванням надоцтової кислоти, пероксиду водню (H2O2) та наночасток металів (нанодисперсного діоксиду титану TiO2, оксиду заліза Fе2O3 та сульфату міді CuSO4), стимулює розвиток ембріонів та забезпечує підвищення виводимості яєць на 7,7 % відносно контролю. Технологія «GREEN ARTICLE» для передінкубаційної обробки яєць з використанням препарату «Штучна кутикула» призводить до значного зниження кількості патогенних мікроорганізмів на поверхні шкаралупи, що призводить до підвищення виводимості яєць.