Chitosan Hybrid Research Articles

Chitosan-Peptide Composites for Tissue Engineering Applications: Advances in Treatment Strategies.

One of the most well-known instances of an interdisciplinary subject is tissue engineering, where experts from many backgrounds collaborate to address important health issues and improve people's quality of life. Many researchers are interested in using chitosan and its derivatives as an alternative to fabricating scaffold engineering and skin grafts in tissue because of its natural abundance, affordability, biodegradability, biocompatibility, and wound healing properties. Nanomaterials based on peptides can provide cells with the essential biological cues required to promote cellular adhesion and are easily fabricated. Due to such worthy properties of chitosan and peptide, they find their application in tissue engineering and regeneration processes. The implementation of hybrids of chitosan and peptide is increasing in the field of tissue engineering and scaffolding for improved cellular adherence and bioactivity. This review covers the individual applications of peptide and chitosan in tissue engineering and further discusses the role of their conjugates in the same. Here, the recent findings are also discussed, along with studies involving the use of these hybrids in tissue engineering applications.

Zearalenone degradation by peptide-based enzyme mimics attached membrane reactor: Performance and mechanism

Zearalenone (ZEN) is a nonsteroidal estrogenic mycotoxin with widespread contamination. Inspired by lactone hydrolases, a peptide-based enzyme mimetic material for degrading ZEN was developed by combining serine, histidine and glutamate (S/H/E) catalytic triad with pro-hydrophobic self-assembling sequences and oxyanion hole site. Chitosan hybrid membranes were prepared, followed by immobilizing enzyme mimic on the membrane surface to fabricate biocatalytic membrane reactor. The membrane reactor, with good thermal stability and high catalytic activity after repeated use, can be applied to the degradation of ZEN in food. Computer simulation studies of the degradation mechanism indicated that the carbon atom on the lactone bond within ZEN molecule was susceptible to catalytic triplex attack, leading to lactone bond broken, followed by spontaneous decarboxylation to produce dihydroxyphenyl derivatives with greatly reduced binding capacity to the estrogen receptors. This kind of peptide-based enzyme mimetic material would be very promising in degrading mycotoxins in food safety field.

Fabrication of Kaolin Loaded Chitosan Hybrid Nanofibers for Recovery of Precious Palladium Cations from Aqueous Solution

Fabrication of Kaolin Loaded Chitosan Hybrid Nanofibers for Recovery of Precious Palladium Cations from Aqueous Solution

Preparation of silver-based metal-organic framework and chitosan hybrid material for removing drug and dye

Pharmaceuticals and personal care products and dyes have low biodegradability and high toxicity, seriously threaten the human health and ecological environment. Therefore, seeking effective removal methods has become the focus of research. In this study, silver-based metal-organic framework (Ag-MOF) and chitosan (CS) hybrid adsorbent (Ag-MOF-CS) was synthesized via solvothermal one-pot synthesis to remove diclofenac sodium (DCF) and acid Red 1 (AR1) from water for the first time. The morphology and structure of Ag-MOF-CS were confirmed by various characterizations. The effect on adsorption was investigated by changing the adsorbent dosage, pH and other conditions. The adsorption kinetics, adsorption isotherms and thermodynamics were analyzed. Ag-MOF-CS showed a high adsorption capacity. And the maximum adsorption capacity of Ag-MOF-CS for DCF and AR1 was 351.75 mg/g and 678.83 mg/g, respectively. The adsorbent bound to DCF and AR1 may via electrostatic forces, π-π interactions, hydrogen bonding. Even after four cycles of Ag-MOF-CS, the DCF removal can still be higher than 80 %. The eco-friendly Ag-MOF-CS demonstrated significant potential for utilization in treating wastewater.

Hybrid chitosan/HKUST-1 hydrogel with freezing and thawing modification as sustainable porous material for removal and selective separation of dye mixtures

A hybrid chitosan monolithic hydrogel (CS) was modified by incorporating with HKUST-1 and fabricated using an in-situ growth method. The resulting hydrogel CS/HKUST-1 was further modified to enhance the porous structure, in which the porous converted from close pores to interconnected porous structures. The chemical and physical characteristics of the modified hydrogel were examined using various characterization techniques like BET, SEM, EDX, FTIR, XRD, Zeta potential, and compression test to understand the effect of the modification. The outcomes displayed that this method provided homogeneous dispersing of HKUST-1 crystals within the chitosan hydrogel matrix, which can improve the material's specific surface area and adsorption capacity. The CS/HKUST-1 monolithic hybrid hydrogel's permeability is further enhanced by the freezing and thawing process and the produced hydrogel is called FCS/HKUST-1, which helps to open the hydrogel's pores and significantly increases its surface area. FCS/HKUST-1 hydrogels display higher mechanical properties without fracture after compression with elastic properties, whereas the CS/HKUST-1 hydrogel crashed at around 47 % compression strain. Consequently, the obtaining dual modified chitosan/ HKUST-1 hydrogel (FCS/HKUST-1) was evaluated as an adsorbent to eliminate Eosin Y (EY) dye from its aqueous solution and the adsorption mechanism was proposed as multilayer adsorption depending on the Freundlich isotherm model. The modified monolithic hydrogel displayed an excellent maximum adsorption capacity with EY dye about 625 mg·g−1. The FCS/HKUST-1 hydrogel showed extremely selective removal for anionic dye higher than cationic dye, which allows facile separation of the dyes with different charges in a mixture solution. The outcomes also demonstrate that the modified hydrogel would be an eco-friendly material, which shows excellent recyclability, indicating that this material considerable promising adsorbent in water treatment.

Chitosan hybrid nanomaterials: A study on interaction with biomimetic membranes

This study examined the influence of nanomaterials (NMs) on the organization of membrane lipids and the resulting morphological changes. The cell plasma membrane is heterogeneous, featuring specialized lipid domains in the liquid-ordered (Lo) phase surrounded by regions in the liquid-disordered (Ld) phase. We utilized model membranes composed of various lipids and lipid mixtures in different phase states to investigate the interactions between the NMs and membrane lipids. Specifically, we explored the interactions of pure chitosan (CS) and CS-modified nanocomposites (NCs) with ZnO, CuO, and SiO2 with four lipid mixtures: egg-phosphatidylcholine (EggPC), egg-sphingomyelin/cholesterol (EggSM/Chol), EggPC/Chol, and EggPC/EggSM/Chol, which represent the coexistence of Ld, Lo, and Ld/Lo, respectively. The data show that CS NMs increase the membrane lipid order at glycerol level probed by Laurdan spectroscopy. Additionally, the interaction of CS-based NMs with membranes leads to an increase in bending elasticity modulus, zeta potential, and vesicle size. The lipid order changes are most significant in the highly fluid Ld phase, followed by the Lo/Ld coexistence phase, and are less pronounced in the tightly packed Lo phase. CS NMs induced egg PC vesicle adhesion, fusion, and shrinking. In heterogeneous Lo/Ld membranes, inward invaginations and vesicle shrinking via the Ld phase were observed. These findings highlight mechanisms involved in CS NM-lipid interactions in membranes that mimic plasma membrane heterogeneity.

In-situ honeycomb spheres for enhanced enzyme immobilization and stability

This paper presents a promising heterogeneous biocatalyst-immobilized cellulase system, which can facilitate the targeted catalytic conversion of biomass to reducing sugars. Magnetic Zeolite Imidazole Framework-8/chitosan (Fe3O4/ZIF-8/CS, FZC) hybrid honeycomb microspheres were synthesized to immobilize cellulase using an in-situ growth strategy. The enhancement of immobilization efficiency induced by each constituent of the composite carrier was explored. This exploration was grounded in varying combinations of components within the honeycomb composite spheres, revealing the indispensability of ZIF-8. Through in-situ assembly, the composite material presents a microsphere with an internal honeycomb structure and multilevel pores. Effective immobilization, temperature-responsive conformational change, and cyclic stability were achieved due to the scaling effect of the channel, extensive specific surface area, and abundant amino group interactions enable immobilized enzymes to maintain excellent performance under challenging operating conditions, demonstrating an enhanced structure-performance relationship. With the obtained immobilized cellulase, we achieved a high load (461.67 mg/g) of cellulase and a significant reducing sugar yield (328.39 mg/g) from corncob waste. In addition, we investigated the enhancement effect of thermal activation on the immobilized enzyme activity of composite carrier. The results showed that the appropriate heat treatment was conducive to the activation of FZC immobilized enzyme with a reducing sugar yield 1.61 times higher than that of the control. The strategy provided in this study promotes the development of immobilized biocatalysts and provides a useful reference for the further design of biocatalytic systems.

Ternary Metal Oxide–Chitosan Hybrids for Efficient Photocatalytic Remediation of Organic Pollutants from Wastewater

Ternary Metal Oxide–Chitosan Hybrids for Efficient Photocatalytic Remediation of Organic Pollutants from Wastewater

Construction of Mesenchymal Stem Cell-Derived Artificial Human Urinary Bladder: A Preliminary Study

Objective: The present study aimed to obtain the required cells and select a suitable scaffold material for constructing an artificial bladder using the tissue engineering approach. 
 Materials and methods: The convenience of obtaining human adipose tissue-derived stem cells (hADMSCs) was used in this study. It was attempted to differentiate these cells into smooth muscle cells (SMC), which are present along the wall of the bladder. Urothelial cells were enzymatically isolated from tissue biopsies. Synthetic (poly-lactide co-glycolic acid, PLGA) and natural (chitosan) polymers were used in scaffold fabrication using a tissue engineering approach.
 Results: In the cellular experiments, urothelial cells couldn’t be cultured in polystyrene culture vessels in vitro and required a support material to maintain viability. Better results were obtained with the feeder layer. The hADMSCs exhibited the expected morphological changes in the serum-rich medium content in the SMC differentiation experiments. Chitosan, biocompatible and biodegradable, was mixed with PLGA as an alternative scaffold combination.
 Conclusion: This study indicated that hADMSCs-derived smooth muscle cells and biopsy-isolated urothelial cells cultured on hybrid chitosan–PLGA scaffolds with appropriate physical properties could serve as a suitable model for tissue-engineered artificial bladder construction.

Conjugated microporous polymer chitosan hybrid materials for enhanced pollutant removal

Highly tailorable adsorbents are the key to addressing specialized pollution problems, such as the release of pharmaceuticals into our wastewaters. Conjugated microporous polymers (CMPs) pose a platform with modifiable functionality and pore system, allowing pollutant-customized adsorption. Herein novel phenanthren-dione based monomers are coupled to triethynylbenzene (TEB) forming new CMPs. These are compared with two literature-known, also TEB based CMPs regarding their adsorption properties towards the model drug diclofenac. To improve the handleability and enhance the adsorption the CMPs are integrated into chitosan gel beads, enabling simple removal using a sieve. The drying process of the beads turned out to significantly influence the swelling. Vacuum-dried beads exhibited 4–8 times higher water uptake than air-dried beads. Further, it proved beneficial to use hexanoyl modified chitosan as hybrid matrix, since it shows even further improved swelling capabilities, leading to a facilitated diffusion of the diclofenac through the chitosan matrix. This resulted in a notable enhancement of the adsorption efficiency of the CMPs, while concurrently exerting control over the adsorption process through the customization of swelling properties.

Decellularized nucleus pulposus matrix/chitosan hybrid hydrogel combined with nucleus pulposus stem cells and GDF5-loaded microspheres for intervertebral disc degeneration prevention

BackgroundIntervertebral disc degeneration (IDD) is considered an important pathological basis for spinal degenerative diseases. Tissue engineering is a powerful therapeutic strategy that can effectively restore the normal biological properties of disc units. In this study, hydrogels loaded with growth/differentiation factor 5 (GDF5) and stem cells were combined to provide an effective strategy for nucleus pulposus regeneration.MethodsNucleus pulposus stem cells (NPSCs) were obtained by low-density inoculation and culture, and their stem cell characteristics were verified by flow cytometry and a tri-lineage-induced differentiation experiment. A decellularized nucleus pulposus matrix (DNPM) and chitosan hybrid hydrogel was prepared, and GDF5-loaded poly(lactic-co-glycolic acid) (PLGA) microspheres were incorporated into the hydrogels to obtain a composite hydrogels with GDF5-loaded microspheres. Taking bone marrow mesenchymal stem cells (BMSCs) as a reference, the effect of composite hydrogels with GDF5-loaded microspheres on the chondrogenic differentiation of NPSCs was evaluated. A model of intervertebral disc degeneration induced by acupuncture on the tail of rats was constructed, and the repair effect of composite hydrogels with GDF5-loaded microspheres combined with NPSCs on IDD was observed.ResultsStem cell phenotype identification, stemness gene expression and tri-lineage-induced differentiation confirmed that NPSCs had characteristics similar to those of BMSCs. The rat DNPM and chitosan hybrid hydrogels had good mechanical properties, and the GDF5-loaded microspheres sustainably released GDF5. NPSCs grew normally in the composite hydrogels and gradually expressed a chondrocyte phenotype. Animal experiments showed that the composite hydrogels with GDF5-loaded microspheres combined with NPSCs effectively promoted nucleus pulposus regeneration and that the effect of the hydrogels on the repair of IDD was significantly better than that of BMSCs.ConclusionGDF5-loaded microspheres combined with DNPM/chitosan composite hydrogels can effectively promote the differentiation of NPSCs into nucleus pulposus-like cells and effectively preventIDD.

Antimicrobial and Antibiofilm Potential of PEGylated Chitosan-Based Nano-Antibiotics Against Multidrug-Resistant E Coli Strains

Antimicrobial and Antibiofilm Potential of PEGylated Chitosan-Based Nano-Antibiotics Against Multidrug-Resistant E Coli Strains

Mitochondrion-targeted carboxymethyl chitosan hybrid nanoparticles loaded with Coenzyme Q10 protect cardiac grafts against cold ischaemia‒reperfusion injury in heart transplantation

BackgroundHeart transplantation (HT) has been approved as an optimal therapeutic regimen for patients with terminal-stage cardiac failure. However, cold ischaemia‒reperfusion (I/R) injury remains an unavoidable and outstanding challenge, which is a major factor in early graft dysfunction and an obstacle to long-term survival in HT. Cold I/R injury induces cardiac graft injury by promoting mitochondrial dysfunction and augmenting free radical production and inflammatory responses. We therefore designed a mitochondrion-targeted nanocarrier loaded with Coenzyme Q10 (CoQ10) (CoQ10@TNPs) for treatment of cold I/R injury after cardiac graft in a murine heterotopic cardiac transplantation model.MethodsHybrid nanoparticles composed of CaCO3/CaP/biotinylated-carboxymethylchitosan (CaCO3/CaP/BCMC) were synthesized using the coprecipitation method, and the mitochondria-targeting tetrapeptide SS31 was incorporated onto the surface of the hybrid nanoparticles through biotin-avidin interactions. Transmission electron microscopy (TEM) and dynamic light scattering (DLS) analysis were used for characterisation. In vitro, the hypoxia-reoxygenation model of H9c2 cells was employed to replicate in vivo cold I/R injury and treated with CoQ10@TNPs. The impact of CoQ10@TNPs on H9c2 cell injury was assessed by analysis of oxidative damage and apoptosis. In vivo, donor hearts (DHs) were perfused with preservation solution containing CoQ10@TNPs and stored in vitro at 4 °C for 12 h. The DHs were heterotopically transplanted and analysed for graft function, oxidative damage, apoptosis, and inflammatory markers 1 day post-transplantation.ResultsCoQ10@TNPs were successfully synthesized and delivered CoQ10 to the mitochondria of the cold ischaemic myocardium. In vitro experiments demonstrated that CoQ10@TNPs was taken up by H9c2 cells at 4 °C and localized within the mitochondria, thus ameliorating oxidative stress damage and mitochondrial injury in cold I/R injury. In vivo experiments showed that CoQ10@TNPs accumulated in DH tissue at 4 °C, localized within the mitochondria during cold storage and improved cardiac graft function by attenuating mitochondrial oxidative injury and inflammation.ConclusionsCoQ10@TNPs can precisely deliver CoQ10 to the mitochondria of cold I/R-injured cardiomyocytes to effectively eliminate mitochondrial reactive oxygen species (mtROS), thus reducing oxidative injury and inflammatory reactions in cold I/R-injured graft tissues and finally improving heart graft function. Thus, CoQ10@TNPs offer an effective approach for safeguarding cardiac grafts against extended periods of cold ischaemia, emphasizing the therapeutic potential in mitigating cold I/R injury during HT. These findings present an opportunity to enhance existing results following HT and broaden the range of viable grafts for transplantation.

Development and Characterization of Bentonite/Hydroxyapatite-Based Chitosan Hybrid Composite for Effective Removal of Methylene Blue

Development and Characterization of Bentonite/Hydroxyapatite-Based Chitosan Hybrid Composite for Effective Removal of Methylene Blue

Photothermal Fibrous Chitosan/Polydopamine Sponge for Intraoperative Hemostasis and Prevention of Tumor Recurrence in Hepatocellular Carcinoma Resection.

Hepatectomy, a surgical procedure for liver cancer, is often plagued by high recurrence rates worldwide. The recurrence of liver cancer is primarily attributed to microlesions in the liver, changes in the immune microenvironment, and circulating tumor cells in the bloodstream. To address this issue, a novel intervention method that combines intraoperative hemostasis with mild photothermal therapy is proposed, which has the potential to ablate microlesions and improve the immune microenvironment simultaneously. Specifically, the integrated strategy is realized based on the fibrous chitosan/polydopamine sponge (CPDS), which is constructed from shearing-flow-induced oriented hybrid chitosan fibers and subsequent self-assembly of polydopamine. The CPDS demonstrates high elasticity, excellent water absorption, and photothermal conversion performance. The results confirm the efficient hemostatic properties of the fibrous CPDS in various bleeding models. Notably, in subcutaneous and orthotopic postoperative recurrence and metastasis models of hepatocellular carcinoma, the fibrous CPDS significantly inhibits local tumor recurrence and distant metastasis. Moreover, the combination with lenvatinib can substantially enhance the antitumor effect. This comprehensive treatment strategy offers new insights into hepatectomy of liver cancer, representing a promising approach for clinical management.

Innovating superparamagnetic chitosan hybrid nanoparticles for a high-efficiency separation of oil from oil–water emulsions

Abstract In an era marked by rapid industrialization and heightened automobile usage, the demand for crude oil has surged, inducing ecological degradation and resource depletion. Effective management of intricate oily wastewater presents a formidable challenge. While diverse methods like gravity separation, centrifugation, and membrane techniques are employed for oil-water separation, gravity separation is the prevailing choice, yet limited to unstable emulsions. These methods often involve toxic substances harmful to marine life. Our research focuses on separating oil microemulsions in aqueous solutions. This study explores the application of superparamagnetic chitosan coagulants, revealing an optimal 10 ml dosage for peak efficiency. Aiming for rapid oil separation, we achieved a breakthrough with just 30 minutes, establishing a new benchmark. Rigorous VSM testing solidified the particles' magnetic capabilities, augmented through size reduction. Notably, at a 15% oil concentration, a remarkable 99.26% efficiency in oil separation was achieved, offering potential in microbiology, medicine, and drug delivery systems.

Synthesis and Characterization of Environmentally Friendly Chitosan-Arabic Gum Nanoparticles for Encapsulation of Oregano Essential Oil in Pickering Emulsion.

The encapsulation of bioactive agents through the utilization of biodegradable nanoparticles is a topic of considerable scientific interest. In this study, microcapsules composed of chitosan (CS) and Arabic gum (GA) nanoparticles were synthesized, encapsulating oregano essential oil (OEO) through Pickering emulsions and subsequent spray drying. The optimization of hybrid chitosan and Arabic gum (CS-GA) nanoparticle formation was carried out via complex coacervation, followed by an assessment of their behavior during the formation of the emulsion. Measurements of the size, contact angle, and interfacial tension of the formed complexes were conducted to facilitate the development of Pickering emulsions for encapsulating the oil under the most favorable conditions. The chitosan-Arabic gum capsules were physically characterized using scanning electron microscopy and fitted to the Beerkan estimation of soil transfer (BEST) model to determine their size distribution. Finally, the OEO encapsulation efficiency was also determined. The optimum scenario was achieved with the CS-GA 1-2 capsules at a concentration of 2% wt, featuring a contact angle of 89.1 degrees, which is ideal for the formation of oil/water (O/W) emulsions. Capsules of approximately 2.5 μm were obtained, accompanied by an encapsulation efficiency of approximately 60%. In addition, the hybrid nanoparticles that were obtained showed high biodegradability. The data within our study will contribute fundamental insights into CS-GA nanoparticles, and the quantitatively analyzed outcomes presented in this study will hold utility for forthcoming applications in environmentally friendly detergent formulations.

Synthesis and characterization of a novel magnetic chitosan–nickel ferrite nanocomposite for antibacterial and antioxidant properties

A novel nanomagnet modified with nickel ferrite nanoparticles (NPs) coated with hybrid chitosan (Cs–NiFe2O4) was synthesized using the co-precipitation method. The resulting nanomagnets were characterized using various techniques. The size of the nanomagnetic particles was estimated to be about 40 nm based on the transmission electron microscopy (TEM) image and X-ray diffraction analysis (XRD) pattern (using the Debye–Scherrer equation). Scanning electron microscopy (SEM) images indicated that the surface of Cs–NiFe2O4 NPs is flatter and smoother than the uncoated NiFe2O4 NPs. According to value stream mapping (VSM) analysis, the magnetization value of Cs–NiFe2O4 NPs (17.34 emu/g) was significantly lower than NiFe2O4 NPs (40.67 emu/g). The Cs–NiFe2O4 NPs indicated higher antibacterial properties than NiFe2O4 NPs and Cs. The minimum inhibitory concentrations of Cs–NiFe2O4 NPs against S. aureus and E. coli were 128 and 256 mg/mL, respectively. Antioxidant activity (evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging test) for NiFe2O4 NPs and Cs–NiFe2O4 NPs at the concentration of 100 µg/mL were 35% and 42%, respectively. Consequently, the synthesized Cs–NiFe2O4 NPs can be proposed as a viable material for biomedical applications.

Hybrid chitosan gold nanoparticles for photothermal therapy and enhanced cytotoxic action of 6-mercaptopurine on breast cancer cell line

BackgroundOne of the most popular anti-inflammatory and anti-leukemic medications is 6-mercaptopurine, along with its riboside derivatives. Because of their potent adverse effects and limited biological half-life, they are rarely used. These problems might be solved by a novel medication delivery technique based on gold nanoparticles (AuNPs). In present work, gold/chitosan nanohybrid was manufactured and assessed for photothermal therapy as well as a drug carrier to minimize the unwanted harmful effects of 6-Mercaptopurine (6-MP). We estimate loading of 6-MP on gold nanoparticles by chitosan reduction (Au@CS NPs) creating (Au@CS-6MP).ResultsAuNPs were green sensitized in one step via chitosan. UV–visible spectroscopy, Zeta potential, TEM, FTIR spectroscopy, and HPLC technique for loading efficiency were used to characterize AuNPs and Au@CS-6MPC NPS. Our results estimate that AuNPs and Au@CS-6MPC NPS with small sizes of 16 ± 2 and 20 ± 4 nm, respectively, and Zeta potential 53.6 ± 5.2 and 55 ± 3 mV, respectively, and loading efficiency of 52% were achieved. Cytotoxicity of the Au@CS-6MPC NPs was significantly increased compared to free 6MP with IC50 1.11 µM. Cell viability was inhibited in AuNPs exposed to DPSS laser light, reaching 10% inhibition after 8 min.ConclusionsThe prepared Au@CS-6MPC NPs resulted in an additive effect in therapeutic managing of breast cancer. It can be predicted that this nanocomposite along with synergistic effect of laser light will definitely result in better therapeutic efficacy and reduced side effects of 6-MP in a combination photothermal chemotherapy treatment. This combination can be explored as future alternative for cancer therapy.

Chitosan/hybrid ceria supported nickel nanoparticles: Study of magnetic and optical properties, photodegradation of rhodamine B, and antimicrobial activity, with DFT

In this study, we explore the magnetic and optical properties of nickel nanoparticles decorated with chitosan/hybrid ceria (iron doped ceria) alongside their potential use in the photodegradation of rhodamine B dye. To support our experimental results, we use Density Functional Theory (DFT) analysis. In addition, the antibacterial activity of the synthesized nanocomposite is evaluated. The hybrid nanoparticles have interesting magnetic and optical characteristics, which make them a viable option for a variety of applications. The chitosan hybrid ceria-nickel nanoparticles also successfully photodegrade the dye rhodamine B, suggesting their potential uses in environmental remediation. Additionally, the nanocomposite antimicrobial activity against the gram-positive Streptococcus pneumoniae and gram-negative Klebsiella pneumonia, which cause a variety of diseases including pneumonia, urinary tract infection, sinusitis, osteomyelitis, meningitis, and septic arthritis, was evaluated demonstrating the nanocomposite ability to fight infectious infections. Overall, this paper thoroughly evaluates the nickel nanoparticles supported on the chitosan hybrid ceria, emphasizing their various features and prospective uses.