In this cross-sectional study, we aimed to identify subgroups of job satisfaction and health-related quality of life (HRQoL) in Chinese male nurses and examine the factors associated with subgroup profiles. A convenience sample of 626 Chinese male nurses were enrolled from January to October 2021. Latent profile analysis was performed to identify profiles based on self-esteem, psychological resilience, social support, neuroticism, perceived prejudice, occupational stress, job satisfaction, and HRQoL. Chi-squared tests were used to examine predictors of profiles. Results indicated that a three-profile model provided the best fit: low job satisfaction and health (9.90%), moderate job satisfaction and health (64.06%), and high job satisfaction and health (26.04%). The average number of monthly night shifts was negatively correlated with the male nurses' subgroups. Psychological resilience, social support, and neuroticism were the key factors associated with the HRQoL of male nurses, while perceived prejudice, occupational stress, and self-esteem were the key factors associated with job satisfaction. Nurse administrators could improve their job satisfaction and health by reducing perceived prejudice, and job stress, increasing organizational support and resilience.