Jujube Research Articles

Determination of antioxidant activity, chlorophyll and carotenoids content of Kappaphycus alvarezii: Comparison of seaweed with traditional Chinese Medicines

After the pandemic of COVID-19, there has been a growing interest in food safety concerns. Besides consuming wild-type animals, another focus is the synthetic compounds used in food preparation. Various reports show that antioxidant mechanisms might be closely related to decreasing the risk of disease infection. On 18 March 2020, Malaysia had the first Movement Control Order (MCO) that all communities must stay home. Public also questions whether natural food, mainly traditional Chinese medicine, can act as an antioxidant agent. Therefore, spectrophotometric procedures for determining the antioxidant capacities of selected samples were conducted. This method is based on scavenging 2, 2 diphenyl 1-picryl hydroxyl (DPPH) by adding a radical species or an antioxidant that decolourises the DPPH solution. This study aimed to compare, using the DPPH assay, the antioxidant level of Kappaphycus alvarezii to other traditional Chinese medicines (TCM) such as Andrographis paniculata, Lycium chinense and Ziziphus jujube. This study characterises the antioxidant activity of traditional Chinese medicines associated with carotenoid and total chlorophyll content from seaweed. Antioxidant analysis has shown that the highest antioxidant property was observed in the solvent extract of L. chinense. The DPPH assay provides an easy and rapid way to evaluate potential antioxidants in selected samples.

Quantitative analysis of biochemical characteristics and anti-cancer properties in MCF-7 breast cancer cell line: a comparative study between Ziziphus jujube honey and commercial honey.

There is increasing evidence that honey has anti-inflammatory, antioxidant, and anti-cancer effects. This study aims to assess and contrast the cytotoxic, anti-metastatic, and apoptotic effects of Ziziphus jujube honey and commercial honey on MCF7 cells. Two honey samples, Ziziphus jujube (JH) and commercial honey (CH), were categorized into high and low groups based on their phenolic content, antioxidant capacity, and diastase activity(PAD score). The viability and migration ability of MCF-7 cells treated with JH and CH were evaluated. Also, quantitative polymerase chain reaction (Q-PCR) was performed to assess the effect of the two honey samples on the expression of Bax, p53,p21 and Bcl-2 genes. JH had a total phenolic content of 606.4 ± 0.1µg gallic acid equivalent/mg, while CH had a value of 112.1 ± 0.09µg gallic acid equivalent/mg. The total antioxidant capacity of the two samples was compared. It was 203.5 ± 10.5µM/l in JH and 4.6 ± 10.5 µM/l in CH. In addition, JH had a diastatic activity of 524.1 ± 0.25 U/l, while CH had a value of 209.7 ± 0.56 U/l. According to the results, JH had a high PAD value, while CH had a low PAD value. Cell viability was measured using the results of the MTT assay. The results showed that JH inhibited the growth of MCF-7 cells more strongly (IC50 of 170 ± 4.2µg/ml) than CH (IC50 of 385.3 ± 4.5µg/l). The scratch assay showed that treatment with JH decreased the migration rate of MCF-7 cells in a dose-dependent manner compared to the CH and control groups. In addition, the results of q-PCR analysis showed significant upregulation of Bax, p53 and p21genes and downregulation of Bcl-2 gene in the JH-treated group compared to the CH and control groups. These results showed that honey with an increased content of phenolic compounds, antioxidant capacity, and diastatic activity has anticancer properties by effectively suppressing tumor development. This suppression occurs via several mechanisms, including suppression of proliferation and metastasis, and promotion of apoptosis.

Sour Jujube (Ziziphus jujuba var. spinosa): A Bibliometric Review of Its Bioactive Profile, Health Benefits and Trends in Food and Medicine Applications.

Research on the comprehensive utilization of sour jujube and its beneficial properties to human health has attracted extensive attention. This study aims to conduct a bibliometric analysis of the bioactive profile of sour jujube and future trends in applications. The research advancements within this field from 2000 to 2023 were addressed using the Web of Science database and VOSviewer. Among the 322 results, the most frequent keywords of bioactivity are flavonoids, antioxidants, saponins, insomnia, polyphenols, terpenoids and anti-inflammatory; the most studied parts of sour jujube are seeds, fruits and leaves; the published articles with high citations mainly focus on identification, biological effects and different parts distribution of bioactive compounds. The bioactivity of various parts of sour jujube was reviewed considering their application potential. The seeds, rich in flavonoids, saponins and alkaloids, exhibit strong effects on central nervous system diseases and have been well-developed in pharmacology, healthcare products and functional foods. The pulp has antioxidant properties and is used to develop added-value foods (e.g., juice, vinegar, wine). The leaves can be used to make tea and flowers are good sources of honey; their extracts are rich sources of flavonoids and saponins, which show promising medicinal effects. The branches, roots and bark have healing properties in traditional folk medicine. Overall, this study provides a reference for future applications of sour jujube in food and medicine fields.

The Transcriptional Regulatory Mechanisms Exploration of Jujube Biological Traits through Multi-Omics Analysis

Jujube (Ziziphus jujuba Mill.) stands as a pivotal fruit tree with significant economic, ecological, and social value. Recent years have witnessed remarkable strides in multi-omics-based biological research on jujube. This review began by summarizing advancements in jujube genomics. Subsequently, we provided a comprehensive overview of the integrated application of genomics, transcriptomics, and metabolomics to explore pivotal genes governing jujube domestication traits, quality attributes (including sugar synthesis, terpenoids, and flavonoids), and responses to abiotic stress and discussed the transcriptional regulatory mechanisms underlying these traits. Furthermore, challenges in multi-omics research on jujube biological traits were outlined, and we proposed the integration of resources such as pan-genomics and sRNAome to unearth key molecules and regulatory networks influencing diverse biological traits. Incorporating these molecules into practical breeding strategies, including gene editing, transgenic approaches, and progressive breeding, holds the potential for achieving molecular-design breeding and efficient genetic enhancement of jujube.

Effect of different drying methods on the drying characteristics, chemical properties and antioxidant capacity of Ziziphus jujuba var. Spinosa fruit

The fruit of Ziziphus jujuba var. Spinosa (ZJF) is a byproduct of the processing of the Suanzaoren commonly used in traditional Chinese medicine. Due to its high nutrient value, ZJF has been one of the most promising food sources. However, they are highly perishable, which severely limits the effective use of ZJF. To find a suitable drying method that facilitates the storage and processing of ZJF while maximizing the preservation of its nutrients and high biological activity, three drying methods, hot air drying (HD), infrared drying (ID), and vacuum drying (VD), were applied to ZJF samples at temperatures of 50, 60, and 70 °C in the present study. The drying kinetics, water activity, and content of active ingredients, including total polysaccharides (TPC), total flavonoids (TFC), total triterpene acids (TTAC), betulinic acid, oleanolic acid, ursolic acid, and antioxidant capacity were analyzed. The results showed that ID required the shortest drying time; with increasing temperature and time, the TPC decreased, the TTAC increased, and the TFC showed a dynamic trend. Furthermore, there was no significant difference between the samples and the antioxidant capacity. This study revealed that ID at 70 °C for 2 h is a suitable drying method for ZJF.

Nontargeted metabolomics and enzyme inhibitory and antioxidant activities for chemical and biological characterization of jujube (Ziziphus jujuba) extracts

The chemical and biologically active characterization of jujube samples (fruits, cores, and leaves) were carried out by the integrated nontargeted metabolomics and bioassay. Firstly, collision cross-section values of active compounds in jujubes were determined by ultrahigh-performance liquid chromatography coupled with ion mobility quadrupole time-of-flight mass spectrometry. Then, a multidimensional statistical analysis that contained principal component analysis, partial least squares-discriminant analysis and hierarchical clustering analysis was employed to effectively cluster different tissues and types of jujubes, making identification more scientific. Furthermore, angiotensin-converting enzyme (ACE) and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) were used to evaluate the quality of jujubes from a double activity dimension. The analytical results obtained by using ACE and DPPH to evaluate the quality of jujube were different from multivariate statistics, providing a reference for the application of jujube. Therefore, integrating chemical and biological perspectives to evaluate the quality of jujube provided a more comprehensive evaluation and effective reference for clinical needs.

Investigation of ziziphus jujube seeds as a new source of taxifolin and silibinin with three different extraction methods

Many food wastes are potentially worth examining due to the beneficial phenolic substances they contain. Jujube seeds are richer than jujube fruit pulp in terms of bioactive substances, especially phenolics. In this study, the amounts of taxifolin, silibinin, kaempferol, quercetin, rutin, and apigenin in jujube seed extracts obtained by conventional Soxhlet (CSE), ultrasonic assisted (UAE), and supercritical fluid extraction (SFE) methods were investigated. The method yields were examined by comparing the amounts of obtained bioactive substances. Total amounts of these bioactive compounds were 837.91 µg/g, 242.24 µg/g, and 51.44 µg/g by UAE, CSE, and SFC methods, respectively. These substances were found to be 470.82 ± 8.21 µg/g taxifolin, 134.4 ± 3.15 µg/g silibinin, 112.52 ± 3.36 µg/g kaempferol, 60.04 ± 0.25 µg/g quercetin and 54.63 ± 0.69 µg/g rutin in UAE extraction, which was the most efficient method. Taxifolin and silibinin were predominant phenolics. The high levels of taxifolin and silibinin in jujube seeds are known to prevent, treat and reduce the risk of cancer and chronic diseases. As a promising assessment, the examined jujube seed oil appears to be a usable source for food supplementation or pharmaceutical purposes with its high taxifolin and silibinin potential.

Multi-View Jujube Tree Trunks Stereo Reconstruction Based on UAV Remote Sensing Imaging Acquisition System

High-quality agricultural multi-view stereo reconstruction technology is the key to precision and informatization in agriculture. Multi-view stereo reconstruction methods are an important part of 3D vision technology. In the multi-view stereo 3D reconstruction method based on deep learning, the effect of feature extraction directly affects the accuracy of reconstruction. Aiming at the actual problems in orchard fruit tree reconstruction, this paper designs an improved multi-view stereo structure based on the combination of remote sensing and artificial intelligence to realize the accurate reconstruction of jujube tree trunks. Firstly, an automatic key frame extraction method is proposed for the DSST target tracking algorithm to quickly recognize and extract high-quality data. Secondly, a composite U-Net feature extraction network is designed to enhance the reconstruction accuracy, while the DRE-Net feature extraction enhancement network improved by the parallel self-attention mechanism enhances the reconstruction completeness. Comparison tests show different levels of improvement on the Technical University of Denmark (DTU) dataset compared to other deep learning-based methods. Ablation test on the self-constructed dataset, the MVSNet + Co U-Net + DRE-Net_SA method proposed in this paper improves 20.4% in Accuracy, 12.8% in Completion, and 16.8% in Overall compared to the base model, which verifies the real effectiveness of the scheme.

The 'Candidatus Phytoplasma ziziphi' effectors SJP1/2 negatively control leaf size by stabilizing the transcription factor ZjTCP2 in jujube.

Phytoplasmas manipulate host plant development to benefit insect vector colonization and their own invasion. However, the virulence factors and mechanisms underlying small-leaf formation caused by jujube witches' broom (JWB) phytoplasmas remain largely unknown. Here, effectors SJP1 and SJP2 from JWB phytoplasmas were identified to induce small-leaf formation in jujube (Ziziphus jujuba). In vivo interaction and expression assays showed that SJP1 and SJP2 interacted with and stabilized the transcription factor ZjTCP2. Overexpression of SJP1 and SJP2 in jujube induced ZjTCP2 accumulation. In addition, the abundance of miRNA319f_1 was significantly reduced in leaves of SJP1 and SJP2 transgenic jujube plants and showed the opposite pattern to the expression of its target, ZjTCP2, which was consistent with the pattern in diseased leaves. Overexpression of ZjTCP2 in Arabidopsis promoted ectopic leaves arising from the adaxial side of cotyledons and reduced leaf size. Constitutive expression of the miRNA319f_1 precursor in the 35S::ZjTCP2 background reduced the abundance of ZjTCP2 mRNA and reversed the cotyledon and leaf defects in Arabidopsis. Therefore, these observations suggest that effectors SJP1 and SJP2 induced small-leaf formation, at least partly, by interacting with and activating ZjTCP2 expression both at the transcriptional and the protein level, providing new insights into small-leaf formation caused by phytoplasmas in woody plants.

Comparative analysis of jujube and sour jujube gave insight into their difference in genetic diversity and suitable habitat.

Jujube (Ziziphus jujuba var. jujuba Mill.) and sour jujube (Z. jujuba var. spinosa (Bunge) Hu ex H.F.Chow.) are economically, nutritionally, and ecologically significant members of the Rhamnaceae family. Despite their importance, insufficient research on their genetics and habitats has impeded effective conservation and utilization. To address this knowledge gap, we conducted plastome sequencing, integrated distribution data from China, and assessed genetic diversity and suitable habitat. The plastomes of both species exhibited high conservation and low genetic diversity. A new-found 23 bp species-specific Indel in the petL-petG enabled us to develop a rapid Indel-based identification marker for species discrimination. Phylogenetic analysis and dating illuminated their genetic relationship, showing speciation occurred 6.9 million years ago, in a period of dramatic global temperature fluctuations. Substantial variations in suitable climatic conditions were observed, with the mean temperature of the coldest quarter as the primary factor influencing distributions (-3.16°C-12.73°C for jujube and -5.79°C to 4.11°C for sour jujube, suitability exceeding 0.6). Consequently, distinct conservation strategies are warranted due to differences in suitable habitats, with jujube having a broader distribution and sour jujube concentrated in Northern China. In conclusion, disparate habitats and climatic factors necessitate tailored conservation approaches. Comparing genetic diversity and developing rapid species-specific primers will further enhance the sustainable utilization of these valuable species.

Systematic exploration of Ziziphus species collection and gene identification influencing physiological traits using genotype-by-sequencing technology.

The Ziziphus genus, belonging to the Rhamnaceae family, holds significant economic, nutritional, and medicinal value. However, much remains to be discovered about its diversity and physical characteristics. Factors such as growth, resilience to changes, disease resistance, and unique features contribute to the quality of Ziziphus species. This study aims to investigate the genomes of 200 genotypes from five Ziziphus species: Ziziphus jujuba (Zj), Ziziphus nummularia (Zm), Ziziphus oxyphylla (Zx), Ziziphus mauritiana (Zm), and the cultivated variety Ziziphus jujube var. jujube, collected from Pakistan and China. Our goal is to identify single nucleotide polymorphisms (SNPs) associated with eight different traits and understand the genetic diversity within the selected Ziziphus species and their genotypes. Using high-quality SNPs obtained through genotype-by-sequencing (GBS), we conducted population structure, phylogenetic, and principal coordinates analyses, identifying a total of 10,945 clean SNPs. These genotypes were categorized into two groups, A and B. Natural Ziziphus variants in Pakistan, specifically Z. jujuba and Z. nummularia, exhibited high levels of genetic diversity and polymorphic information content (PIC) of 0.46 and 0.41, respectively, compared to other species. Furthermore, we identified 15 influential candidate genes that play crucial roles in regulating agronomic traits, such as fruit width and diameter, leaf width, plant height, and stem diameter within this group. This study provides valuable insights that can be utilized in Ziziphus breeding efforts.

COAGULATION POTENTIALS OF THE LEAVES OF DESERT DATES, GUIERA, JUJUBE TREE, KAMEL’S FOOT AND MAHOGANY

Coagulation activity in water and waste water treatment tends to reduce turbidity by agglomerating the dissolved and suspended particles thereby making them dense enough to settle. Alluminium phosphate is the common solution that is used to achieve coagulation. In case of lacking aluminum phosphate, an alternative has to be considered. In this study, leaves of five trees are considered. The trees include Jujube Tree (Prunus Domestica), Desert dates (Balanites Eagyptiaca), Kamel’s Foot (Piliostigma reticulatum; P. Thonningii), Guiera (Guiera Senegalensis) and Mahogany (Khaya Senegalensis). Leaves of these trees were dried and grinded to powder before they were dissolved in water. Extract of these solution were used for coagulation potentialities with a concentration of 2 ml per liter. Turbidity of the water was tested before coagulation and was found to be 233 NTU. The results of the turbidity after coagulation showed that the water has 53 NTU, 69 NTU, 70 NTU, 74 NTU and 700 NTU for Mahogany, Jujube, Guiera, Desert Dates and Kamel’s Foot respectively. Drinking water limit of 5NTU for turbidity in accordance to W.H.O has not been achieved in all the tests made

The Sequence Characteristics and Binding Properties of the Odorant-Binding Protein SvelOBP1 from Sympiezomias velatus (Coleoptera: Curculionidae) to Jujube Volatiles.

Sympiezomias velatus (Chevrolat) (Coleoptera: Curculionidae) has caused serious damage on jujube trees (Ziziphus jujuba Mill) in northern China. Semiochemicals emerging from the host are essential in the process of insects identifying and localizing the host. The highly expressed odorant-binding protein 1 of S. velatus (SvelOBP1) was assumed to play a possible role in the recognition of host volatiles. In this study, SvelOBP1 was cloned based on the antennal transcriptome of S. velatus. The recombinant SvelOBP1 protein was expressed in Escherichia coli and purified by Ni-NTA resin. The predicted protein SvelOBP1 belonged to a classic OBP subfamily. The expression patterns revealed that SvelOBP1 was mainly expressed in the antennae of both males and females, whereas the expression of SvelOBP1 in other body parts could be neglected. The fluorescence binding assay indicated that SvelOBP1 displayed very strong binding affinities to dibutyl benzene-1,2-dicarboxylate and (Z)-hex-3-en-1-ol (Ki = 6.66 ± 0.03 and 7.98 ± 0.06 μM). The molecular docking results showed that residues Trp114, Phe115 and Asp110 may be involved in binding to both dibutyl benzene-1,2-dicarboxylate and (Z)-hex-3-en-1-ol and may have a great impact on odorant recognition of S. velatus. Our results provide evidence that SvelOBP1 might participate in the olfactory molecular perception of S. velatus and would promote the development of pest attractants for S. velatus control.

In Vitro Wound-healing Activity of Ziziphus jujuba and Its Anti-inflammatory Effects in Rats

In Vitro Wound-healing Activity of Ziziphus jujuba and Its Anti-inflammatory Effects in Rats

Effect of Infrared-Combined Hot Air Intermittent Drying of Jujube (Zizyphus jujuba Miller) Slices: Drying Characteristics, Quality, and Energy Consumption Dimensions

The objective of this research was to investigate the effect of infrared-combined hot air intermittent drying (IIRHAD) on energy consumption, drying characteristics, and the quality of jujube slices. The water content of jujube slices decreased from 0.267 g/g to 0.05 g/g during the experiment, and the infrared heating plate’s temperature was fixed at 70 °C while the hot air temperature was fixed at 50 °C. Nine different intermittent ratios were used to dry jujube slices, and the results showed that intermittent treatment had varying effects on drying characteristics, energy consumption, and quality. In comparison to infrared-combined hot air drying (IRHAD), the effective drying time of red jujube slices was reduced by 40 to 100 min, the energy consumption decreased by 11.91% to 34.34%, and there were also varying degrees of improvement in the quality indicators. It was discovered that excessively long or short active drying and tempering periods had a negative impact on the drying process. Therefore, these factors should be further broken down and improved in the future. This research holds great importance for the future advancement and widespread use of IIRHAD in fruit and vegetable materials.

Cultivation and morphology of jujube (Ziziphus Jujuba Mill.) in the Qi River Basin of Northern China during the Neolithic Period

This transition from gathering to cultivation is a significant aspect of studying early agricultural practices. Fruit trees are an essential component of food resources and have played a vital role in both ancient and modern agricultural production systems. The jujube (Ziziphus jujuba Mill.), with its long history of cultivation in northern China, holds great importance in uncovering the diet of prehistoric humans and understanding the origins of Chinese agricultural civilization. This paper focuses on the domestication of jujube by analyzing the morphology of jujube stones found in three Neolithic sites in northern China's Qi River basin, Zhujia, Wangzhuang, and Dalaidian. The measurements of these jujube kernels are compared with those found in other areas of northern China, as well as modern jujube kernels that were collected. The measurements revealed that the length-to-diameter (L/D) ratio of sour jujube kernels ranged from 1.36 to 1.78, whereas the L/D ratio of cultivated jujube stones varied between 1.96 and 4.23. Furthermore, jujube stones obtained from Zhujia and Wangzhuang sites exhibit pointed ends and possess an elongated oval or narrow oval shape overall, which is indicative of clearly artificial domestication traits. Therefore, this study suggests that jujube was selected and cultivated as an important food supplement in the Qi River basin no later than around 6200 BP.

Influence of Grass Mulching and Organic Fertilizer Addition on Soil Microbial Community Composition and Function in a ‘Dongzao’ Jujube (Ziziphus jujuba Mill. ‘Dongzao’) Orchard

Influence of Grass Mulching and Organic Fertilizer Addition on Soil Microbial Community Composition and Function in a ‘Dongzao’ Jujube (Ziziphus jujuba Mill. ‘Dongzao’) Orchard

Innovative Bicultured Lactic–Acetic Acid Co-fermentation Improves Jujube Puree’s Functionality and Volatile Compounds

Jujubes (Ziziphus jujuba Mill), characterized by a rich profile of bioactive compounds, have been historically less exploited due to their unappealing sensory characteristics when dried, including delayed bitterness and a limited shelf life when fresh. Co-fermented jujube puree has emerged as a strategy for enhancing its functional food potential. This study examined the impact of 8-day bicultured Junzao jujube puree, employing both commercial and indigenous Chinese lactic and acetic acid bacteria. Our investigation encompassed an assessment of functionality (cellular profile, antioxidant properties, color, free amino acids, phenolic profiling, volatiles elucidation using headspace-solid phase microextraction gas chromatography–mass spectrometry (HS-SPME-GC-MS), aroma analysis using electronic nose), and microstructural analysis using scanning electron microscopy (SEM). Viable counts of bicultured purees showed probiotic effects exceeding 6–7 log CFU/mL. Strong positive correlations were observed between phenolic compounds (chlorogenic acid, rutin, p-coumaric acid) and antioxidant capacities (ABTS-RSA and DPPH-RSA). The darker color of raw jujube puree was modified, exemplified by a significant (p < 0.05) negative correlation between overall color difference and cyanidin 3-O-rutinoside (R2 = −0.768). Purees, particularly those containing bicultures of Lactobacillus helveticus Lh 43 and Acetobacter pasteurianus Ap-As.1.41 HuNiang 1.01 exhibited the highest potential free amino acid content (157.17 ± 1.12 mg/100 g FW) compared to the control (184.03 ± 1.16 mg/100 g FW) with a distinctive formation of L-methionine in biculture of Lactiplantibacillus plantarum Lp 28 and A. pasteurianus Ap-As.1.41 HuNiang 1.01. The phenolic profile of Lacticaseibacillus casei Lc 122 and A. pasteurianus Ap-As.1.41 HuNiang 1.01 increased by 22.79% above the control (48.34 mg/100 g FW) while biculture: L. helveticus Lh 43 and A. pasteurianus Ap-As.1.41 HuNiang 1.01 were enhanced by 4.37%, with the lowest profile in Lp. plantarum Lp 28 and A. pasteurianus Ap-As.1.41 HuNiang 1.01 (46.85 mg/100 g FW). The electronic nose revealed the predominant presence of sulfur, terpenes, and alcohol sensor bioactives in the fermented purees. HS-SPME-GC-MS analysis identified 80 volatile compounds in the bicultured purees, with esters constituting the major group (42%). Furthermore, SEM analysis unveiled massive microscopic alterations in the bicultured purees compared to the unfermented puree. These results collectively demonstrated that lactic–acetic acid co-fermentation serves to biovalorize Junzao jujube puree, enhancing its organoleptic appeal and extending its shelf life.

Yanıt yüzey yöntemi kullanılarak Ziziphus jujube Mill yapraklarından fenolik bileşenlerin ultrason destekli ekstraksiyonunun optimizasyonu

The phenolic compounds in jujube (Ziziphus jujuba Mill) leaf were extracted using ultrasound assisted extraction (UAE) for potential of antioxidant food additive in this study. The extraction factors such as methanol concentration, temperature and time were optimized using response surface methodology (RSM) to maximize of the total phenolic content (TPC), 2,2 -diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and phenolic profile in jujube leaf extracts (JE). The best possible range for methanol concentration (25-50%), duration of ultrasound (20-40 min), ultrasonic temperature (40–60 °C) were obtained using the Box-Behnken design (BBD). The optimum extraction parameters were obtained with 25% methanol concentration, 20 min duration of ultrasound and 49.89 °C ultrasonic temperature. Ellagic, caffeic, rosmarinic acid, and rutin were determined as major phenolics in JE under optimal extraction parameters. The results revealed that UAE is an effective pretreatment for extracting bioactive ingredients from JE as potential functional food additive. Furthermore, RSM is an effective method for optimizing the UAE factors.

Development of InDel markers based on chloroplast DNA for the identification of Ziziphus jujuba Mill. cultivars

Development of InDel markers based on chloroplast DNA for the identification of Ziziphus jujuba Mill. cultivars