Chinese Crab Research Articles

Analysis and Optimized Design of Rigid–Flexible Coupling Characteristics of Crab Apple Picking Machines and Crab Apple Trees

In recent years, with the gradual expansion of the planting area for Chinese crab apple trees, the traditional method of picking crab apples has become inadequate due to its large workload, low efficiency, and high cost. To address this issue, this paper presents the design of a trunk-type vibration picker specifically for crab apple picking. The design process began with a modal analysis and a harmonic response analysis of a crab apple tree using Ansys Workbench. Through these analyses, the optimal excitation frequency for the crab apple tree was determined to be 12.7 Hz. Subsequently, the picker was designed, and its key components underwent modal and stress analyses. A comparison revealed no resonance between the picker and the key components, ensuring the feasibility of the picker. To further evaluate the performance of the picker, a rigid–flexible coupling analysis was conducted using Adams. This analysis determined the acceleration response curves of the lateral branch points of the crab apple tree at various excitation frequencies: 9.7 Hz, 12.7 Hz, 15.7 Hz, and 18.7 Hz. Finally, field trials were carried out to validate the reliability of the data obtained from Adams. The results showed that the lateral branch point acceleration increased with higher excitation frequencies. However, when the optimal excitation frequency of 12.7 Hz was reached, the lateral branch point acceleration decreased. This indicated that the most effective vibration frequency for picking crab apples was 12.7 Hz.

The second known stygomorphic freshwater crab from China, Phasmon typhlops gen. nov. et sp. nov. (Crustacea, Decapoda, Potamidae), diverged at the beginning of the Late Miocene.

A new genus and new species of blind freshwater cave crab are described from Chongzuo City, Guangxi Zhuang Autonomous Region, China based on morphology and mitochondrial 16S rDNA sequences. The new genus, Phasmongen. nov., is established for P. typhlopssp. nov., which is only the second blind cave crab known from China and East Asia. The combination of a very wide carapace, overall depigmentation, reduced orbits and vestigial unpigmented eyes of Phasmon immediately separates it from all known potamid genera. Molecular divergence estimates based on 16S rDNA suggest that the lineage to which the new genus belongs diverged from other potamids at the beginning of the Late Miocene (10.8 million years ago), much earlier than other Chinese cave crabs.

Effects of Feeding Ice Fish and Feed on the Flavor of Chinese Crab

Effects of Feeding Ice Fish and Feed on the Flavor of Chinese Crab

Intestinal Tract Microbe Communities Associated with Horseshoe Crabs from Beibu Gulf, China.

Until now, there has been little research on the intestinal microbial community of horseshoe crabs. To fill this gap, we investigated the microbiome composition of the Chinese horseshoe crab, Tachypleus tridentatus, and the mangrove horseshoe crab, Carcinoscorpius rotundicauda. We sequenced the 16S rRNA gene of intestinal bacterial species and compared the microbial community structure and diversity. Next, we show that the total effective bacterial sequence was 36,865 reads, and the average annotated operational taxonomic unit (OTU) number was 240. Through hierarchical clustering analysis and principal coordinate analysis samples from two horseshoe crab species, we found that the intestinal flora of the same horseshoe crab species was relatively concentrated, while the microbiome of a different horseshoe crab species were significantly separated. Cluster analysis showed that two samples, one from Chinese horseshoe crabs and one from mangrove horseshoe crabs, had similar microbial community structure, while other samples were relatively discrete. The gut microbiota of the mangrove horseshoe crab were dominated by the phyla Tenericutes (42.71%), Firmicutes (24.27%), and Proteobacteria (20.39%), while the top three phyla in the Chinese horseshoe crab intestinal tract were Tenericutes (57.19%), Proteobacteria (22.14%), and Bacteroidetes (7.38%). To intuitively understand the similarity and overlap of the OTU composition of each group, we performed Venn diagram analysis. The two species shared 284 OTUs, accounting for 81.8% of the total. This indicates that although there is high similarity between mangrove and Chinese horseshoe crab in gastrointestinal microbial community structure, there are also some differences, which deserve further discussion.

Risks and critical issues related to the discovery on the market of unauthorized live alien species on the Italian territory: Chinese crab (Eriocheir sinensis).

Eriocheir sinensis, Chinese Crab or Chinese Mitten Crab is a catadromous species belonging to the Varunidae family, native to river and estuarine areas of North and South East China and Korea. At European level, E. sinensis is widespread in the main water basins of Central and Northern Europe and, since 2016, it has been included in the list of invasive species important for the European Union and subjected to confinement and eradication measures which include the prohibition of collection, transit and placing on the market of live specimens (Regulation (EC) N° 1143/2014). The Chinese Crab can represent a significant danger for the local ecosystem and for the native biota as well as contributing to the appearance of hydrogeological instability phenomena resulting from the intense excavation and erosion of the riverbanks.The first finding of 5 kg of live specimens of Eriocheir sinensis was recorded in the official control by the UFS (Functional Simple Unit) veterinary public health and food safety of the ASL Toscana Centro at an ethnic catering establishment. The specimens were subjected to seizure, photographed, identified morphologically, and subjected to euthanasia and destruction in accordance with the European requirements for welfare and management of animal by-products.From the sanitary point of view, the dangers associated with the consumption of this crab are mainly biological and chemical therefore, risk communication is fundamental, not only at the level of the competent authorities in the sector, but also for the food business operators.

Effects of Elevated pCO2 on the Survival and Growth of Portunus trituberculatus.

Identifying the response of Portunus trituberculatus to ocean acidification (OA) is critical to understanding the future development of this commercially important Chinese crab species. Recent studies have reported negative effects of OA on crustaceans. Here, we subjected swimming crabs to projected oceanic CO2 levels (current: 380 μatm; 2100: 750 μatm; 2200: 1500 μatm) for 4 weeks and analyzed the effects on survival, growth, digestion, antioxidant capacity, immune function, tissue metabolites, and gut bacteria of the crabs and on seawater bacteria. We integrated these findings to construct a structural equation model to evaluate the contribution of these variables to the survival and growth of swimming crabs. Reduced crab growth shown under OA is significantly correlated with changes in gut, muscle, and hepatopancreas metabolites whereas enhanced crab survival is significantly associated with changes in the carbonate system, seawater and gut bacteria, and activities of antioxidative and digestive enzymes. In addition, seawater bacteria appear to play a central role in the digestion, stress response, immune response, and metabolism of swimming crabs and their gut bacteria. We predict that if anthropogenic CO2 emissions continue to rise, future OA could lead to severe alterations in antioxidative, immune, and metabolic functions and gut bacterial community composition in the swimming crabs through direct oxidative stress and/or indirect seawater bacterial roles. These effects appear to mediate improved survival, but at the cost of growth of the swimming crabs.

Determination of Perfluorinated Compounds Level of Domestic and Imported Crabs in South Korea

Perfluorinated compounds(PFCs) are an organic fluorine compound that hydrogens of all of carbons compound were replaced by fluorine. PFCs were demonstrated for toxic effects by various studies. PFCs tend to accumulate because of the long half-life in vivo. Research on PFCs level of food material in South Korea is still in insufficient. In this study, we analyzed 19 PFCs in crab meat with LC-MSMS.A total of 151 crabs were collected from fish market of South Korea. The samples taken included countries of origin information; South Korea (n=104), China (n=37) and India (n=10). Crab samples were homogenized with water. Each sample was weighed (1g) and spiked 20 µl internal standard. PFCs were extracted from samples using ion-pairing extraction after enzyme hydrolysis. Quantitative analysis was performed with liquid chromatography–tandem mass spectrometry(LC-MS/MS).As result, PFOA was detected in South Korean and Chinese crab samples. PFTrDA and PFDS levels were higher in Chinese crab than South Korean Crabs.Although the legal criteria of PFCs in food is not yet defined, the levels of PFCs in South Korean crabs were similar to or less than other studies performed in aboard; Belgium (PFOS ranged from 93 to 292 ppb), China (PFOS up to 82.2 ppb) and Japan (PFOA ranged from 5.6 to 14 ppb).

A new optimization particle filtering navigation location method for aquatic plants cleaning workboat in crab farming

Chinese river crabs are important aquatic products in China, and the accurate operation of aquatic plants cleaning workboat is an urgent need for solving various problems in the aquaculture process. In order to achieve the accurate navigation positioning, this article introduces the visual-aided navigation system and combines the advantages of particle filter in nonlinear and non-Gaussian systems. Meanwhile, the generalized regression neural network is used to adjust the particle weights so that the samples are closer to the posterior density, thus avoiding the phenomenon of particle degradation and keeping the diversity of particles. In order to improve the network performance, the fruit fly optimization algorithm is introduced to adjust the smoothing factor of transfer function for the generalized regression neural network model layer. On this basis, the location filtering navigation method based on fruit fly optimization algorithm-generalized regression neural network-particle filter is proposed. According to the simulation results, the meanR of root-mean-square error of the proposed fruit fly optimization algorithm-generalized regression neural network- particle filter method decreases by 12.39% and 6.87%, respectively, compared with those of particle filter and generalized regression neural network methods, and the meanT of running time decreases by 16.04% and 9.14%, respectively. From the repeated experiments on the aquatic plants cleaning workboat in crab ponds, the latitude error of the proposed method decreases by 23.45% and 12.68%, respectively, and that the longitude error decreases by 29.11% and 17.65%, respectively, compared with those of particle filter and generalized regression neural network methods. It is proved that our proposed method can effectively improve the navigation positioning accuracy of aquatic plants cleaning workboat.

Fatty acids from controlled feeding as dietary markers of juvenile Chinese horseshoe crab, Tachypleus tridentatus

The potential use of fatty acid (FA) profile as a dietary tracer in juvenile Chinese horseshoe crab Tachypleus tridentatus was assessed in a feeding experiment. Selected feed was provided to the juveniles at various growth stages according to their food sources in the wild: larval and adult brine shrimp Artemia salina for the first six months, followed by a mixture of short-neck clam Ruditapes philippinarum and greasyback shrimp Metapenaeus ensis for a further six months. Juvenile horseshoe crab haemolymph and tissues of book gill, opisthosomal spine, chelicera and swimming leg were sampled after the feeding experiment. The FA profiles revealed that the juvenile haemolymph and various tissues had a total of 23–24 FA components. The major FA components consisted of saturated (palmitic and stearic) at 22–34%, monosaturated (oleic) at 7–34% and polyunsaturated (eicosapentaenoic and arachidonic) FAs at 9–27%. According to principal component analysis, the FA signatures of juvenile haemolymph were greatly influenced by the shift of food sources from brine shrimp to clam and shrimp meat, whereas the FA profiles of different tissues were more resistant to the nutrient alteration. The present findings confirmed that the FA profile of both juvenile haemolymph and different tissues reflected the FA composition of recent and previous diets, respectively; and thus may be useful in indicating the foraging information of juvenile horseshoe crabs in the field.

The Establish of Four Kinds of Chinese Primary Economic Crab Species Fatty Acid Fingerprints

In order to establish our common economic crabs fatty acid fingerprints, the total lipid and phospholipid fatty acid composition of our common economic crabs were determined and analyzed by GC-MS. The results show that: the highest proportion of Eriocheir sinensis is monounsaturated fatty acid (MUFA), while the highest proportion of the other three crab is polyunsaturated fatty acid (PUFA). The percentage of EPA+DHA in Total fat of four kinds of crab is between 7.58%~36.82% .The ratio of EPA and DHA of three kinds of sea crab is higher than it in Eriocheir sinensis. Through the comparative analysis of the fatty acid composition of the total lipid and the phospholipids, we identified seven characteristic fat acids of the four species of crabs.They are C16:0, C18:1n-9, C18:2n-6, C18:3n-3, C20:5n-3, C22:6n-3,and PUFA(PL). Chemical information of four kinds of crabs in lipid composition can be mastered through fatty acids fingerprints of four kinds of crabs which used seven kinds of characteristic fatty acid as index.

Effect of salinity on the metabolism of female Chinese crabs, Eriocheir sinensis

We evaluated the effect of salinity on the metabolism of female Chinese crabs(Eriocheir sinensis).The crabs,body weight(55.59 ± 6.24) g,were held at salinities of 0,6,12,18 and 24,(16 ± 1)℃,and we measured oxygen consumption,CO2 production,and ammonia-N excretion.Using this data,we calculated the respiratory quotient,oxygen-nitrogen ratio,energy consumption rate,and metabolic substrate ratio.Salinity had a significant effect on the oxygen consumption rate and ammonia–N excretion rate(P0.05).Between salinity 0–24,the rates of oxygen consumption and ammonia–N excretion both decreased as salinity increased.We observed a similar pattern for CO2 production and energy consumption,but the differences were not significant relative to the control group at salinity 6(P0.05).Between salinity 12–24,CO2 production and energy consumption were significantly lower than in the control group.Salinity had no effect on the respiratory quotient and oxygen–nitrogen ratio(P0.05).Our results suggest that lipids are used preferentially as an energy source.Furthermore,proteins were utilized to a much greater extent than carbohydrates.The proportion of lipids in the metabolic substrate increased as salinity increased.Conversely,the carbohydrate metabolic substrate ratio decreased as salinity increased,whereas the protein metabolic substrate ratio remained constant.At salinity 24,the ratio of protein,carbohydrate,and lipid used as metabolic substrate was 6.4:14.6:79.0.During the reproductive migration,the female Chinese crab migrates from fresh water into the Yangtze River estuary.Thus,individuals must adjust to the higher salinity and modify their energy metabolism.Our results suggest that energy consumption decreases after entering salt water,allowing female crabs to divert energy into reproduction.

English

English

Salinity regulates N-methylation of phosphatidylethanolamine in euryhaline crustaceans hepatopancreas and exchange of newly-formed phosphatidylcholine with hemolymph

Phosphatidylcholine (PC), the main phospholipid in eukaryotes, is synthesized via two different routes, the phosphatidylethanolamine N-methyl transferase (PEMT) and the CDP-choline pathways. We previously showed in euryhaline fish that salinity impacts the relative contribution of the two pathways for PC biosynthesis, with PEMT pathway being activated in the liver of sea water (SW)-adapted animals. To address the occurrence of such phenomenon in other animals we performed in vivo metabolic studies in two crustacean species: the Chinese crab (Eriocheir sinensis) and the green crab (Carcinus maenas). In both species, the levels of PC and phosphatidylethanolamine in hepatopancreas and hemolymph were not modified by SW-adaptation. In E. sinensis, SW-adaptation activated PC labeling from L-(U-(14)C)-serine in the hepatopancreas and resulted in an increased ratio of PC specific activities between hemolymph and hepatopancreas. In C. maenas, incorporation of L-(3-(3)H)-serine and L-(2-(14)C)-ethanolamine into PC of hepatopancreas was strongly inhibited after acclimation to fresh water (FW). The results show that PC synthesis via the PEMT pathway and its subsequent release into hemolymph are both activated in SW- compared to FW-adapted animals. SW-adaptation also resulted in increased tissue concentrations of betaine and labeling from L-(U-(14)C)-serine, suggesting that the PEMT-derived PC is used for the synthesis of organic osmolytes. The physiological relevance of these observations is discussed.

Effect of salinity on hemolymph osmotic pressure, sodium concentration and Na+-K+-ATPase activity of gill of Chinese crab, Eriocheir sinensis

The effects of salinity on hemolymph osmotic pressure, Na+ concentration and Na+-K+-ATPase activity of gill of Chinese crab Eriocheir sinensis were studied. The results showed that hemolymph osmotic pressure and Na+ concentration increased significantly (P 0.05); However, the protein concentration decreased gradually with the increase of salinity from 0.25 d to 1 d, and then tended to be stable from day 1 to day 15.

Is HSP70 Involved in Acclimation to Cadmium in the Chinese Crab, Eriocheir sinensis ?

Is HSP70 Involved in Acclimation to Cadmium in the Chinese Crab, Eriocheir sinensis ?

Analysis of Genetic Diversity of Processing Apple Varieties

Genetic diversity of 18 processing apple varieties and two fresh varieties were evaluated using 12 simple sequence repeats (SSR) primer pairs previously identified in Malus domestica Borkh. A total of 87 alleles in 10 loci were detected using 10 polymorphic SSR markers selected within the range of 5–14 alleles per locus. All the 20 varieties could be distinguished using two primer pairs and they were divided into four groups using cluster analysis. The genetic similarity (GS) of groups analyzed using cluster analysis varied from 0.14 to 0.83. High acid variety Avrolles separated from other varieties with GS less than 0.42. The second group contained Longfeng and Dolgo from Northeast of China, the inherited genes of Chinese crab apple. The five cider varieties with high tannin contents, namely, Dabinette, Frequin rouge, Kermerrien, M.Menard, and D.Coetligne were clustered into the third group. The fourth group was mainly composed of 12 juice and fresh varieties. Principal coordinate analysis (PCO) also divided all the varieties into four groups. Juice and fresh apple varieties, Longfeng and Dolgo were clustered together, respectively, using both the analyses. Both the analyses showed there was much difference between cider and juice varieties, cider and fresh varieties, as well as Chinese crab apple and western European crab apple, whereas juice varieties and fresh varieties had a similar genetic background. The genetic diversity and differentiation could be sufficiently reflected by combining the two analytical methods.

Tissue-specific cadmium accumulation and metallothionein-like protein levels during acclimation process in the Chinese crab Eriocheir sinensis

Aquatic organisms chronically exposed to cadmium can increase their resistance to a subsequent elevated exposure. In order to investigate mechanisms involved in acclimation process in the Chinese crab Eriocheir sinensis, we compared Cd level as well as metallothionein-like protein (MTLP) content in different tissues after direct acute exposure (i.e. 500 μg Cd L −1 for 3 days), and after acute following chronic (i.e. 10 or 50 μg Cd L −1 for 30 days) exposure. Cadmium accumulation occurred in the following order: anterior gill>hepatopancreas>posterior gill>carapace>hemolymph>muscle. As high concentrations as 188 μg Cd g −1 w.w. were reported in anterior gills and seem to reach a saturation level. In these gills, the highest MTLP induction was observed after a direct acute exposure, for which a correlation with Cd content occurred. However, the Cd-binding potential by MTLPs was exceeded for any exposure condition. In hepatopancreas, the highest Cd level was reported for crabs acclimated during 30 days to 50 μg Cd L −1 before challenging with an acute exposure. Moreover, we showed that MTLPs were induced during the acclimation process. In this organ, MTLPs are theoretically sufficient to bind all Cd. These results suggest that during a chronic exposure to 50 μg Cd L −1, Chinese crabs acquire the capacity to hold more cadmium in hepatopancreas where it can be sequestrated by MTLPs. On the contrary, MTLP induction seems to be a rapid response to acute exposure in anterior gill, but is not sufficient to sequester all Cd. Other sequestration and/or detoxification mechanisms must take place in anterior gill to cope with high Cd levels.

Active Absorption of Cl– and Na+ in Posterior Gills of Chinese Crab Eriocheir Sinensis: Modulation by Dopamine and cAMP

Abstract Isolated posterior gills from the Chinese crab Eriocheir sinensis acclimated to freshwater (FW) were used for perfusion. Dopamine and dibutyryl cAMP (db-cAMP) induced a significant increase of 36Cl– influx. When amiloride, a specific sodium selective channels blocker, was added to the incubation saline, it induced a dramatic decrease of 22Na+ influx, and such an effect could be reversed by dopamine or db-cAMP. In the same experimental condition, we observed that ouabain added to the serosal side induced a marked decrease of the stimulating effect of db-cAMP on 22Na+ uptake across the epithelium to the hemolymph, suggesting that dopamine stimulates Na+ and Cl– influxes via cAMP and Na+/K+-ATPase pathways.

Dopamine D 1 receptors in the gills of Chinese crab Eriocheir sinensis

Our investigations indicate the existence of binding sites for [ 3H]SCH23390 on crude membrane preparations in the anterior and posterior gills of the Chinese crab, Eriocheir sinensis acclimated to freshwater (FW) or seawater (SW). Maximum specific binding in the posterior gills is always higher than in the anterior gills, independent of saline acclimation. K d values are similar in the two regions, suggesting the same affinity of both types of gills for the ligand, either from FW or from SW crabs.

Ionic balance in the freshwater-adapted Chinese crab, Eriocheir sinensis.

Ionic regulation by the gills of the freshwater-adapted Chinese crab, Eriocheir sinensis, was examined. The balance of uptake and loss of NaCl in crabs living in freshwater was established. Urine production was measured directly by cannulating the nephropores. Daily urinary loss of Na+ is equivalent to 16% of the haemolymph Na+ content and is substantially higher than that based on data from indirect measurements reported in the literature. Weight and area of anterior and posterior gills are proportional to body weight. The role of the gills in compensating urinary loss by uptake was determined by analysing changes in Na+ and Cl- concentrations in the external medium in which isolated perfused gills were suspended. In posterior gills, salt loss is quantitatively balanced by NaCl net uptake from an external concentration of 1.3 mmol l(-1) NaCl upwards. The transport constant (Kt) for half maximum saturation of net uptake and saturation of NaCl uptake are 1.5 mmol l(-1) and 4 mmol l(-1), respectively. In contrast to previous studies in which tracer fluxes or transepithelial short-circuit currents were determined, our method of direct ion determination shows that no net uptake of Na+ or Cl- occurs in posterior gills in the absence of the respective counter ion, or when uptake of one ion is blocked by a specific inhibitor. Net uptake of Na+ and Cl- was about equal. We conclude that the uptake of the two ions is coupled. The properties of the branchial ion uptake of E. sinensis correlates with the distribution of this crab in river systems.