Abstract
English
Highlights
The Chinese horseshoe crab (Tachypleus tridentatus) distributes mainly along the coast of the East and South China Sea, extending northward to the Pacific coast of Japan and southward to Southeast Asia (Sekiguchi, 1988; Yang et al, 2007)
Our result showed that no polymerase chain reaction (PCR) bands were achieved with these twenty-two pairs microsatellites primer and DNA extracted from T. tridentatus in PCR reaction in our previous study, which indicated that those primer pairs from L. polyphemus are not suited for the application in T. tridentatus
Microsatellite analyses Mitochondrial analyses (PIC > 0.5) and only two loci (CHR53, CHR91) were deviated from Hardy-Weinberg equilibrium, no significant linkage disequilibrium was detected among them (Table 2), they were suitable for genetic analysis
Summary
The Chinese horseshoe crab (Tachypleus tridentatus) distributes mainly along the coast of the East and South China Sea, extending northward to the Pacific coast of Japan and southward to Southeast Asia (Sekiguchi, 1988; Yang et al, 2007). T. tridentatus was once abundant in the southeast coast of China, distributed from the estuary of Changjiang River to Beibu Bay (Sekiguchi, 1988; Liao and Li, 2001). Microsatellite has been considered as one of the efficient molecular markers that provide population genetic information, due to its abundance, randomly distributed in the genome, highly polymorphic, its codominant inheritance and ease of scoring (Guo and Gui, 2008; Kaya and Yildiz, 2008). Twenty-two microsatellite markers were developed from Limulus polyphemus (King et al, 2004), which revealed significant population difference among two Atlantic coast and one Gulf of Mexico site. Our result showed that no polymerase chain reaction (PCR) bands were achieved with these twenty-two pairs microsatellites primer and DNA extracted from T. tridentatus in PCR reaction in our previous study (not published), which indicated that those primer pairs from L. polyphemus are not suited for the application in T. tridentatus
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