In recent years, many countries implement the TBT in the export trade practice of agricultural products, which has greatly positive influence on the Chinese agricultural products export enterprise. On the basis of green technical innovation concept, the paper had determined the enterprise green technical innovation motivation mechanism, analyzed the influence of TBT on green technical innovation of the enterprise, and integrated Chinese reality to analyze the influence of TBT on green technical innovation of Chinese agricultural enterprise. Finally, based on the above analysis, the paper proposed corresponding suggestions to the Chinese government.