Chinensis Extract Research Articles

Antioxidant Activity and Phenolic Content of Chinese Balsam (Impatiens chinensis)

Plants of the Impatiens genus includes more than 1,000 species. Several of the species are used as medicinal plants and are known to contain the anti‐inflammatory 2‐methoxy‐1,4‐naphthoquinone. Herbal remedies are used to treat insect bites, rashes, and poison ivy contact dermatitis, as well as management of anxiety and stress. Chinese Balsam (Impatiens chinensis) is found in North‐East India, where it is used medicinally for pain relief, promoting blood circulation, and treating urinary infections. However, very little research has been conducted to analyze the phytochemistry of I. chinensis. Here, is described the first report of antioxidant capacity and phenolic content of the seeds, leaves, stems, roots and flowers of I. chinensis.Impatiens chinensis plants were separated into seeds, leaves, stems, roots and flowers, freeze‐dried, and ground into a powder. For antioxidant assays, ground plant powder was extracted using 1% HCl, 90% aqueous methanol for 2 h under shaking. The 2,2‐diphenly‐1picryl‐hydrazyl‐hydrate (DPPH) and (ABTS) assays were used to determine the antioxidant capacity of each extract. Briefly, 10 μL extract was incubated with 10 μM DPPH for 10 min, after which the absorbance was measured spectrophotometrically at 517 nm. For the ABTS assay, 10 μL extract was incubated with 3.5 mM ABTS radical for 30 s. The absorbance at 734 nm was then measured spectrophotometrically. Results from both assays were compared to a trolox standard and calculated as 6‐hydroxy‐2,5,7,8‐tetramethylchroman‐2‐carboxylic acid (trolox) equivalents (TE; μmol/g dry weight). Results from the ABTS assay showed antioxidant activities of 189.5±26.7, 7462±286.3, 195.3±31.6, 85.03±19.7, and 484.1±29.3 TE (mean±SEM) for stems, flowers, leaves, roots, and seeds, respectively. In correlation, the DPPH assay revealed the same pattern of radical scavenging ability in the various tissues, with the flowers exhibiting the highest amount of antioxidant capacity. The stems, flowers, leaves, roots, and seeds exhibited 150.1±6.128, 910.8±31.62, 188.8±10.72, 118.2±13.27, and 486.9±17.81 TE (mean±SEM), respectively.The phenolic content of I. chinensis extracts was determined using the Folin‐Ciocalteu assay. 20 μL extract was incubated with 2N 10 μL extract reagent, ddH2O, and 12.56% sodium carbonate. After 30 min, the absorbance at 750 nm was measured spectrophotometrically. Results were compared to gallic acid as a standard, and the phenolic content was calculated as gallic acid equivalents (GAE; μmol/g dry weight). The phenolic content was 1127±42.6, 5046±151.0, 1206±49.3, 662.2±24.5, and 1270±70.5 GAE (mean±SEM).

The Effect of Supplementing a Novel n-3 Polyunsaturated Fatty Acids Formulation Containing Schisandra chinensis Extract and Vitamin D3 to a Group of Elite Sport Athletes on Competition Related Stress, Anxiety and Self-confidence

The Effect of Supplementing a Novel n-3 Polyunsaturated Fatty Acids Formulation Containing Schisandra chinensis Extract and Vitamin D3 to a Group of Elite Sport Athletes on Competition Related Stress, Anxiety and Self-confidence

Formulation and clinical evaluation of the standardized Litchi chinensis extract for skin hyperpigmentation and aging treatments

IntroductionThe standardized litchi extract had been revealed on phytochemical actives, in vitro and cellular activities against aging and darkening of skin. However, a formulation containing the extract has never been developed as per clinical evaluated. Materials and methodsThe litchi serum was developed, safety and efficacy were clinically evaluated in human volunteers. The stable and none irritated 0.05 and 0.1% litchi serums were randomized-single blind placebo control clinical applied on the inner forearm of 29 volunteers for a consecutive 112 days and monitored by Mexameter® MX18, Cutometer® MPA 580 and Visioscan® VC 98. ResultsSkin lightening efficacy of the 0.1% and 0.05% litchi serum was significantly (P<0.001 and P<0.05) higher than the placebo. Skin elasticity and wrinkle reduction was significantly (P<0.05 and P<0.005) achieved by the 0.1% litchi serum. The efficacy of litchi serums was confirmed by a split-face, randomized, single-blind controlled that the 0.1% litchi serum was significantly (P<0.05) better than the 0.05% one of all examined parameters. ConclusionSafety and efficacy of litchi extract are clinically confirmed for hyperpigmentation and aging of skin treatments.

Antibacterial Effects of Schisandra chinensis Extract on Escherichia coli and its Applications in Cosmetic.

Schisandra chinensis (Turcz.) Baill (S. chinensis), an edible traditional medicine herb, has a strong constitution, which extract has good antibacterial activity. The study investigated its antibacterial properties on E. coli, to find a candidate for the development as new preservative. In vitro antibacterial assay showed that S. chinensis extract (SCE) effectively inhibited the growth of test bacteria with MBC of 18mg/mL. In model cosmetic system of O/W emulsions, SCE possessed a great antibacterial capacity. The growth curves of E. coli treated with SCE exhibited an extended lag phase and restricted log phase. Scanning electron microscopy showed that the treated E. coli cells exhibited wrinkled and withered surfaces, and disappearing outmost layer, suggesting S. chinensis extract can damage S. aureus cell member and wall, in addition, the leakage of periplasm enzyme AKPase and the increased activities of Na+/K+-ATPase and Ca++-ATPase in cell membrane were also consistent with the microscopy results. Moreover, the S. chinensis extract can decrease the activities of dehydrogenase and total ATPase and the content of intracellular proteins, and bind with S. aureus DNA by electrostatic and groove binding. The results indicated that SCE might be a candidate cosmetic preservative for its good antibacterial activity and multiple targets on E. coli.

In Vitro and In Vivo Anti-AChE and Antioxidative Effects of Schisandra chinensis Extract: A Potential Candidate for Alzheimer's Disease.

Acetylcholinesterase (AChE) inhibition and antioxidants are two common strategies for the treatment in the early stage of Alzheimer's Disease (AD). In this study, extracts from nine traditional Chinese medical (TCM) herbs were tested for anti-AChE activity by Ellman's microplate assay and cytotoxicity by CCK-8. Based on its excellent AChE inhibition effect and its lowest cytotoxicity, Schisandra chinensis (SC) extract was selected to do the mechanism research. SC extract protected pheochromocytoma (PC12) cells against H2O2-induced toxicity by improving the cell survival rate in a dose-dependent manner. And it also showed significant free radical (DPPH) scavenging activities, ferric reducing antioxidant power (FRAP), and 2,2′-Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging. To confirm these results, the scopolamine-induced mice models were utilized in this study. Compared with the positive drug (piracetam), SC could also exhibit similar effects to alleviate the mice's cognitive deficits. Moreover, in the mice brain samples, the AChE activity and malondialdehyde (MDA) levels of SC-treatment group both showed a reverse as compared to model group. Taken together, these results all suggested that SC extract may be a potential therapeutic candidate for AD.

Characterization of the Omija (Schisandra chinensis) Extract and Its Effects on the Bovine Sperm Vitality and Oxidative Profile during In Vitro Storage.

Schisandra chinensis is a woody vine native to China, Korea, and Russia, which has been used as a traditional herbal remedy to treat male infertility. As very little information is available concerning its effects on ejaculated spermatozoa, the aim of this study was to investigate the chemical, antioxidant, and antibacterial properties of the S. chinensis berry (Omija) extract followed by an assessment of its in vitro effects on bovine sperm function and oxidative balance. Phytochemical components of the Omija extract were determined by high performance liquid chromatography. The content of polyphenols, flavonoids, and carotenoids was assessed by spectrophotometric protocols. Antioxidant characteristics of the Omija extract were determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and molybdenum-reducing antioxidant power (MRAP) assays. The disc diffusion method and determination of the minimal inhibitory concentration were applied to study the antibacterial properties of Schisandra. Thirty semen samples were exposed to different concentrations of Omija (1, 5, 10, 25, 50, and 75 µg/mL) for 0, 2, and 24 h. Sperm motility, mitochondrial activity, and superoxide and reactive oxygen species production, as well as total antioxidant capacity and oxidative damage to proteins and lipids were determined. Our data reveal that the Omija extract, particularly at a concentration range within 5–50 µg/mL, exhibited dose-dependent motion-promoting and metabolism-enhancing properties, accompanied by significant antioxidant effects. We may conclude that the biomolecules present in the Omija extract such as schisandrins and phenolic molecules offer protection to critical sperm structures against oxidative insults and/or possible bacterial contamination, leading to a higher preservation of mammalian sperm viability and functional activity.

Antibacterial effect of different concentrations of Galla Chinensis extract on cariogenic bacteria in a biofilm model

Antibacterial effect of different concentrations of <i>Galla Chinensis</i> extract on cariogenic bacteria in a biofilm model

Evaluation of flexural strength and antibacterial effect of orthodontic acrylic resins containing Galla chinensis extract.

ABSTRACTObjective: To evaluate different concentrations of Galla chinensis extract (GCE) added to poly(methyl methacrylate) (PMMA), which is widely used for fabrication of removable orthodontic appliances, regarding the effectiveness of this herbal extract on antimicrobial effect and flexural strength of PMMA. Methods: Acrylic resin samples containing 0.4%, 0.8% and 1.6% GCE were prepared. Flexural strength was investigated via three-point flexural strength test for the 15 acrylic resin blocks of each concentration. Disk diffusion test was used to evaluate antibacterial effects of incorporating the same concentrations of GCE into acrylic resin. All these three groups were compared with the control group, with no added GCE, regarding flexural strength and antibacterial properties.Results: Comparison of flexural strength between the three study groups and the control group showed significant differences between the groups (P=0.018). However, there was no significant difference between the groups containing GCE. There were significant differences in antimicrobial activity between the four groups (P=0.026). Conclusion: Within the limitations of this study, it is suggested that incorporation of GCE into PMMA would be beneficial for antimicrobial activity and flexural strength of PMMA, but further studies on other physical properties and antimicrobial effects on other bacterial strain would be beneficial prior to clinical investigations.

Chemical components and functions of Taxus chinensis extract

Taxus chinensis is a National level protected plant and has been for a long time, since it grows slowly and has low regenerative capacity. Consequently, no large base of raw material forest of taxus was formed. This study used Taxus chinensis as the study object, for analyzing the chemical composition of T. chinensis extract via fourier transform infrared spectroscopy (FT-IR) and gas chromatography/mass spectrometry (GC/MS). 37 types of chemical components were detected. These were mainly ethers (asarone, dibutyl phthalate, diisobutyl phthalate), alcohols (nerolidol, Myo-inositol, 4-C-methyl-, trans-Sinapyl alcohol), acids (3,4-dimethoxycinnamic acid, palmitic acid, oleic Acid), flavonoids (macckiain, formononetin), ketone (4-hydroxy-.beta.-ionone, pseudobaptigenin), phenols (methyleugenol), esters (triacetin), aldehydes (sinapinaldehyde), and pyridines (alpha-methylstyrene). This chemical component could be directly or indirectly used for bioengineering, pharmaceutical engineering, the cosmetic industry, and other chemical industries.

Rosa chinensis in vitro cell cultures: a phytocomplex rich of medium molecular weight polysaccharides with hydrating properties

In-vitro cell cultures of selected Rosa chinensis meristematic cells cultivated with an innovative CROP® (Controlled Release of Optimized Plants) platform, allowed obtaining a stable and standardized phytocomplex rich of medium molecular weight polysaccharides. The polysaccharides profile of the rose extract has been analysed with the size exclusion chromatography (HPLC-ELSD-SEC) both in the in vitro extract and in the dried petals of Rosa chinensis. The polysaccharides content in the extract was ≥20%, higher than in the dried petals. The 65–80% of total polysaccharides have a medium molecular weight (1000 Da), known for their moisturizing and anti-age properties. Reconstructed human epidermis in homeostatic conditions was used to evaluate its moisturizing action and the ability to maintain homeostasis. The Rosa chinensis extract increased the Aquaporin-3 expression and cell membrane localization and demonstrated to regulate hydration either in topical and systemic exposure.

Antagonistic Effect of Cuscuta chinensis on a Rat Model with Unilateral Cryptorchidism.

BackgroundThe aim of this study was to investigate the effect of Cuscuta chinensis Lam. on germ cell apoptosis in a rat model of unilateral cryptorchidism.Material/MethodsThirty male SD rats were randomly and equally divided into a control group, a model group, and a Cuscuta group (5.0 g/kg/d) (n=10). The rat model of unilateral cryptorchidism in the model and Cuscuta groups was established by removal of the right gubernaculum, while rats in the control group received no treatment. After modeling, rats in the Cuscuta chinensis group were intragastrically administered Cuscuta chinensis extract (5.0 g/kg/d), while rats in the control group and model group were administered an equal volume of normal saline. After 90 days, all the rats were sacrificed and the testicles were separated and weighed, followed by TUNEL staining to detect germ cell apoptosis, flow cytometry to measure JC-1, ROS, and MDA, and Western blot analysis to evaluate the expression of Bax, Bcl-2, and cleaved caspase3.ResultsNinety days after the operation, Cuscuta chinensis Lam significantly minimized the damage caused by modeling by increasing weight of testis, reducing the germ cell apoptosis, and enhancing the mitochondrial membrane potential of testicles, as shown by levels of JC-1, ROS, and MDA, as well as elevating the level of Bcl-2/Bax and reducing the level of cleaved caspase3 (P<0.05).ConclusionsTreatment with Cuscuta chinensis Lam reduced the germ cell apoptosis in rats with unilateral cryptorchidism, which provides new insight for the development of cryptorchidism therapy in the future.

Neuroprotective Effect of Schisandra Chinensis on Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine-Induced Parkinsonian Syndrome in C57BL/6 Mice.

Schisandra chinensis (Turcz.) Baill. (S. chinensis) is a well-known botanical medicine and nutritional supplement that has been shown to have potential effects on neurodegeneration. To investigate the potential neuroprotective effect of S. chinensis fruit extract, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) was used to induce behavioral disorders and dopaminergic neuronal damage in mice, and biochemical indicators were examined. Male C57BL/6 mice were used to establish the MPTP-induced parkinsonian syndrome model. Open field and rotarod tests were performed to evaluate the overall manifestation of motor deficits and rodent motor coordination. The mice were divided into 8 groups as follows: normal control; MPTP alone (25 mg/kg, i.p.); S. chinensis extract pretreatment (0.5, 1.5, 5 g/kg, p.o.); and S. chinensis extract treatment (0.5, 1.5, 5 g/kg, p.o.). Liquid chromatography coupled to electrochemical detection was used to monitor neurochemicals in the striatum. Tyrosine hydroxylase content was measured by immunohistochemistry, and biochemical antioxidative indicators were used to evaluate the potential neuroprotective effects of S. chinensis fruit extract. The results demonstrated that treatment with S. chinensis fruit extract ameliorated MPTP-induced deficits in behavior, exercise balance, dopamine level, dopaminergic neurons, and tyrosine hydroxylase-positive cells in the striatum of mice. Among the pretreated and treatment groups, a high dose of S. chinensis fruit extract was the most effective treatment. In conclusion, S. chinensis fruit extract is a potential herbal drug candidate for the amelioration and prevention of Parkinson’s disease.

Hypolipidemic effects of herbal extracts by reduction of adipocyte differentiation, intracellular neutral lipid content, lipolysis, fatty acid exchange and lipid droplet motility

An increase in adipose tissue is caused by the increased size and number of adipocytes. Lipids accumulate in intracellular stores, known as lipid droplets (LDs). Recent studies suggest that parameters such as LD size, shape and dynamics are closely related to the development of obesity. Berberine (BBR), a natural plant alkaloid, has been demonstrated to possess anti-obesity effects. However, it remains unknown which cellular processes are affected by this compound or how effective herbal extracts containing BBR and other alkaloids actually are. For this study, we used extracts of Coptis chinensis, Mahonia aquifolium, Berberis vulgaris and Chelidonium majus containing BBR and other alkaloids and studied various processes related to adipocyte functionality. The presence of extracts resulted in reduced adipocyte differentiation, as well as neutral lipid content and rate of lipolysis. We observed that the intracellular fatty acid exchange was reduced in different LD size fractions upon treatment with BBR and Coptis chinensis. In addition, LD motility was decreased upon incubation with BBR, Coptis chinensis and Chelidonium majus extracts. Furthermore, Chelidonium majus was identified as a potent fatty acid uptake inhibitor. This is the first study that demonstrates the selected regulatory effects of herbal extracts on adipocyte function.

The Pathogenicity and Transcriptome Analysis of Methicillin-Resistant Staphylococcus aureus in Response to Water Extract of Galla chinensis.

Aim Antibiotic abuse contributes to the emergence of methicillin-resistant Staphylococcus aureus (MRSA). It is increasingly important to screen new antimicrobial agents for the management of MRSA infections. G. chinensis, a nontoxic Chinese herbal medicine, is considered a potential antibacterial agent. The aim of this study was to investigate the bactericidal effects of the aqueous extracts of G. chinensis on MRSA. The potential mechanisms of G. chinensis aqueous extract inhibition of the pathogenicity of MRSA in vivo are also discussed. Methods G. chinensis aqueous extract was prepared and its antimicrobial activities were examined by determining its minimum inhibitory concentration (MIC). Biofilm biomass was determined by scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). RNA sequencing (RNA-seq) was used to evaluate differentially expressed functional pathways in MRSA treated with G. chinensis aqueous extract. We validated the role of G. chinensis aqueous extract in the invasive ability and pathogenicity of MRSA in vivo using a rat infectious model. Results The results indicated that MRSA was sensitive to the G. chinensis aqueous extracts at concentration of 31.25μg/mL. G. chinensis extract led to a reduction in dextran-dependent aggregation and biofilm formation in MRSA. Based on the transcriptome analysis, G. chinensis aqueous extracts significantly downregulated the gene expression related to biofilm formation and carbohydrate metabolism. G. chinensis aqueous extract inhibited the invasive ability and the pathogenicity of MRSA in vivo. Conclusion The antimicrobial properties of G. chinensis aqueous extract are likely related to its modulation of MRSA biofilm formation and carbohydrate metabolism. G. chinensis aqueous extract is a promising supplementary therapy to lessen or eliminate the use of antibiotics and is a potential tool for the management of MRSA infections.

Comparative Histological Study of Two Different Pulp Capping Agents in Rabbits Teeth

Objective: Evaluation of the histological pulp response following direct pulp capping with Galla Chinensis Extract (GCE) in comparison to Mineral Trioxide Aggregate (MTA) in rabbits’ teeth. Methodology: Intentional pulp exposure on the labial surfaces of permanent central incisors of twenty male rabbits was done. Split mouth technique was utilized for applying the capping material where MTA was used for capping upper and lower right central incisors while, GCE was used for capping upper and lower left central incisors. The teeth were restored by glass inonomer cement (GIC) as permanent restorations. Rabbits were divided randomly into two groups 10 rabbits each according to pulp capping period (two and four weeks). After animal scarification teeth were dissected and prepared for histological evaluation using Hematoxylin Eosin (HE) stains.Results: GCE group showed significantly higher mean dentin bridge thickness than MTA group at four weeks, also it showed significantly higher median inflammatory cell scores than MTA group at both time intervals. Conclusions: GCE is a promising direct pulp capping material with less biological pulp response in comparison to MTA.

Intestinal absorption of phenolic acids in Rhus chinensis extracts by in situ single-pass perfusion model in rats

The intestinal absorption properties of four main effective components(gallic acid, ocinolglucoside, ethyl gallate and penta-O-galloyl-β-D-glucose) in Rhus chinensis extracts were investigated by in situ single-pass intestinal perfusion model in rats. The liquid accumulation of perfusion was corrected by gravimetry. The HPLC method was established to determine the concentration of the four effective components in the intestinal perfusion. It showed significant differences(P<0.05) in absorption rate constant(K_a) and effective permeability(P_(eff)) among the three concentrations of components, and the absorption of the four effective components in different intestinal segments was saturated at high concentrations. At the same concentration, there were significant differences in K_a and P_(eff) of the four components in each intestinal segment(P<0.05). The order of K_a and P_(eff) of the four components in the intestine was penta-O-galloyl-β-D-glucose>ethyl gallate>gallic acid>ocinolglucoside, with significant differences between them(P<0.05). In conclusion, gallic acid, orpheolglucoside, ethyl gallate and pentacyl-glucose could be absorbed in the whole intestine. Their absorption rate and permeation ability were related to the intestinal section and the perfusate concentration. These results indicated potential active transport or facilitated diffusion in the intestinal transport process of the four effective components.

Antioxidant activity of Coridius chinensis extracts on manganese-induced testicular damage in rats.

ABSTRACT Coridius chinensis (C. chinensis) is a traditional Chinese medicine that has been used to treat pain, erectile dysfunction, and other diseases. Our previous study demonstrated that manganese‐induced reproductive damage was partially rescued by a medium dose of C. chinensis treatment in rat. However, the underlying mechanism is unknown. In this study, we found that the weight of reproductive organs and the sperm count in manganese‐exposed rat were partially rescued by C. chinensis extracts (CcE) treatment. The number of apoptotic cells was significantly decreased and the expression of malondialdehyde, cytochrome c, and caspase‐3 in manganese‐exposed rats was significantly decreased after high dose of CcE treatment. Further studies revealed that the activity of superoxide dismutase, total antioxidant capacity, and glutathione peroxidase enzymes was significantly increased in testis tissues and serum of manganese‐exposed rats with high dose of CcE treatment. Taken together, the results of this study suggest that CcE inhibits the Mn2+‐induced apoptosis in testes by inducing the activity of antioxidants.

Preparation and characterization of Juniperus chinensis extract-loaded polyurethane nanofiber laminate with polyurethane resin on polyethylene terephthalate fabric

To maximize the utility of natural extracts, they must be atomized and mixed with other useful materials to ensure their function is realized. We conducted experiments that involved electrospinning a natural extract in a polymer solution to fuse the extracts in polymer nanofibers. The Juniperus chinensis extracts (JCE) were mixed with a polyurethane (PU) solution to make a nanofiber web (PU/JCE), which was laminated with polyethylene terephthalate at 90, 110, and 130 °C (PU/JCE-LC). We analyzed the morphology, air permeability, water vapor transmission, resistance to water penetration, contact coldness (Qmax), and antibacterial properties of the fabricated PU/JCE nanofiber laminate composites. It was found that as the laminating temperature increased, the air and moisture permeability of the PU/JCE nanofiber laminate decreased, and its water resistance and Qmax increased. Also, the antibacterial test confirmed that the laminate provided antimicrobial activity against Staphylococcus aureus and Klebsiella pneumoniae. The results reveal that the combination process of electrospinning and laminating can provide an effective way to assign functionalities of natural extracts, which opens up the possibility of imparting a variety of functionalities from nature into polymeric nanofiber.

Enhanced Growth Performance,Haemato-biochemical and Immune Parameters of Asian Seabass, Lates calcarifer (Bloch,1790) Fed Dietary Supplementation with Polygonum chinense

The present study evaluated the effects of Polygonum chinense extract (PCE) on the growth performance, haemato-biochemical and innate immunity parameters of Asian seabass, Lates calcarifer (Bloch, 1790). The fish with an average weight of 9.5 ± 0.2 g were fed for 60 days using three supplementation diets of PCE at 0.2 %, 0.5 % and 1.0 %, and a non-supplemented diet as a control. Fish fed on 0.5 % PCE diet revealed significant higher body weight gain, specific growth rate, protein efficiency ratio, condition factor, and significantly lower feed conversion ratio. Dietary supplementation with 0.5 and 1.0 % showed a significant increase in red blood cell count and haemoglobin level, but the white blood cell count significantly increased only in 1.0 % diet. The alkaline phosphatase level significantly increased in fish group fed 0.2 and 0.5 % diets, but the aspartate transaminase level significantly decreases in 0.2 and 1.0 % groups. Meanwhile, all PCE supplemented diets showed significant increase in total protein, albumin, globulin, albumin: globulin ratio, total immunoglobulin, lysozyme, respiratory burst and phagocytic activities, and significantly reduced the alkaline transaminase level. These results suggest that dietary P. chinense supplementation at 0.5 % was found to be suitable to enhance the growth performance and immune status of Asian seabass.

Preventive Effect of Bupleurum chinense on Nasal Inflammation via Suppressing T Helper Type 2, Eosinophil and Mast Cell Activation.

Bupleurum chinense is distributed in East Asia and has beenused as a traditional herbal medicine for more than a thousand years. Though B.chinense has been reported to have immunomodulatory, anti-inflammatory, anti-oxidant, hepato-protective, antipyretic, analgesic and antifibrotic effects, its specific effect on allergic rhinitis disease hasnot been clarified. In this study, we investigated the anti-allergic and anti-inflammation effects of B. chinense extract (BCE) in an ovalbumin (OVA)-induced allergic rhinitis (AR) mouse model. Oral administration of BCE in a dose-independent manner regulated the balance of Th1/Th2/Treg cell differentiation in AR mice. Accordingly, BCE attenuated the expression of Th2-related cytokines such as IL-4, IL-5 and IL-13 in nasal lavage fluid (NALF) and nasal tissue and up-regulated the secretion of Th1/Treg cells including IL-10, IL-12 and IFN- . Also, BCE inhibited the formation and migration of eosinophils to the nasal mucosa and NALF, as well as suppressed CCL24, an eosinophil-specific chemoattractant in NALF. The levels of anti-OVA specific IgE and anti-OVA specific IgG1 were decreased, and as a result, the allergic response was attenuated by BCE via inhibiting mast cells accumulation in nasal mucosa and serum histamine release. The nasal allergy symptoms, nasal mucosal swelling, epithelial barrier disruption and mucus hyperplasia were obviously ameliorated. These results suggest that BCE may have therapeutic potential for treating allergic rhinitis through modulating the accumulation and activation of important leukocytes in the immune system such as Th1, Th2, Treg, eosinophils and mast cells.