In inclined narrow and long spaces such as tunnels, the buoyancy-driven flow pattern and the resulting ventilation flow rate are different from those in enclosure-type buildings, because of their large length-to-width aspect ratios. In this paper, the buoyancy-driven flow rate induced by a localized heat source in inclined tunnels was investigated. Theoretical analysis and three series of experiments including non-isothermal or isothermal buoyancy sources, i.e., large-scale fire tests, brine-water tests and helium-air tests were performed. Two typical buoyant flow patterns were identified in the experiments, which are stratified and well-mixed patterns, respectively. Both the stratification pattern and the longitudinal decay rate of temperature rise were proved to be correlated with a dimensionless factor, η=B01/3ΔLsina/(g0′1/2H3/2), which is composed of characteristic parameters referring to both buoyancy source and geometrical configuration. The flow stratification results in stack effect attenuation and consequently ventilation flow rate reduction. A model for predicting the buoyancy-driven flow rate in such spaces was proposed, with special emphasis on the effects of stratification pattern. The predictions were validated with the experimental data, and the necessity for accounting for the stack effect attenuation caused by stratification was proved. The prediction model possesses an explicit expression and can thus provide a convenient tool for design calculations.