Immune System In Children Research Articles

Viral Vectors in Gene Replacement Therapy.

Throughout the years, several hundred million people with rare genetic disorders have been receiving only symptom management therapy. However, research and development efforts worldwide have led to the development of long-lasting, highly efficient, and safe gene therapy for a wide range of hereditary diseases. Improved viral vectors are now able to evade the preexisting immunity and more efficiently target and transduce therapeutically relevant cells, ensuring genome maintenance and expression of transgenes at the relevant levels. Hematological, ophthalmological, neurodegenerative, and metabolic therapeutic areas have witnessed successful treatment of hemophilia and muscular dystrophy, restoration of immune system in children with immunodeficiencies, and restoration of vision. This review focuses on three leading vector platforms of the past two decades: adeno-associated viruses (AAVs), adenoviruses (AdVs), and lentiviruses (LVs). Special attention is given to successful preclinical and clinical studies that have led to the approval of gene therapies: six AAV-based (Glybera® for lipoprotein lipase deficiency, Luxturna® for retinal dystrophy, Zolgensma® for spinal muscular atrophy, Upstaza® for AADC, Roctavian® for hemophilia A, and Hemgenix® for hemophilia B) and three LV-based (Libmeldy® for infantile metachromatic leukodystrophy, Zynteglo® for β-thalassemia, and Skysona® for ALD). The review also discusses the problems that arise in the development of gene therapy treatments, which, nevertheless, do not overshadow the successes of already developed gene therapies and the hope these treatments give to long-suffering patients and their families.

Complex interplay of gut microbiota between obesity and asthma in children.

Obesity is an important risk factor and common comorbidity of childhood asthma. Simultaneously, obesity-related asthma, a distinct asthma phenotype, has attracted significant attention owing to its association with more severe clinical manifestations, poorer disease control, and reduced quality of life. The establishment of the gut microbiota during early life is essential for maintaining metabolic balance and fostering the development of the immune system in children. Microbial dysbiosis influences host lipid metabolism, triggers chronic low-grade inflammation, and affects immune responses. It is intimately linked to the susceptibility to childhood obesity and asthma and plays a potentially crucial transitional role in the progression of obesity-related asthma. This review article summarizes the latest research on the interplay between asthma and obesity, with a particular focus on the mediating role of gut microbiota in the pathogenesis of obesity-related asthma. This study aims to provide valuable insight to enhance our understanding of this condition and offer preliminary evidence to support the development of therapeutic interventions.

Identification of Mercury Content in Children Stunting Patients Aged 0–24 Months in the Regions Small Scale Gold Mine in Krueng Sabee District, Aceh Jaya Regency

Mercury is a type of heavy metal that is included in the category of Hazardous and Toxic Materials (B3) waste because it can harm living things and pollute the environment. Community mining activities are generally known to be the largest contributor of mercury to the environment. In addition, people's mining activities can indirectly result in the destruction of the landscape and vegetation on it. Mercury exposure can have a negative impact on the health of children living in ASGM areas. A reduced immune response or immune system in children living in ASGM areas with a high risk of exposure to mercury and exacerbated by concomitant malnutrition is very at risk of stunting. this study aims to identify mercury content in children stunting patients aged 0–24 months in the region's small-scale gold mine in Krueng Sabee district, Aceh Jaya Regency. The AAS instrument is used to analyze urine mercury levels. Three of the 11 urine samples did not show mercury, while the remaining 08 samples were found to contain mercury, each of the 08 urine samples was found to have mercury levels exceeding the acceptable threshold (7μg/L) Human Biomonitoring (HBM), while three twenty-nine samples had urine mercury levels below the threshold. This study concluded that 88% of stunted toddlers were found to contain mercury that exceeded the threshold. Meanwhile, 03 urine samples did not contain mercury.

Saliva contact during infancy and allergy development in school-age children.

Parent-child saliva contact during infancy might stimulate the child's immune system for effective allergy prevention. However, few studies have investigated its relation to allergy development in school-age children. We sought to investigate the relationship between parent-child saliva contact during infancy and allergy development at school age. We performed a large multicenter cross-sectional study involving Japanese school children and their parents. The self-administered questionnaires including questions from the International Study of Asthma and Allergies in Childhood were distributed to 3570 elementary and junior high school children in 2 local cities. Data were analyzed for the relationship between saliva contact during infancy (age <12 months) and the risk of allergy development, specifically eczema, allergic rhinitis, and asthma. For detailed Methods, please see the Methods section in this article's Online Repository at www.jacionline.org. The valid response rate was 94.7%. The mean and median age of children was 10.8± 2.7 and 11 (interquartile range, 9-13) years, respectively. Saliva contact via sharing eating utensils during infancy was significantly associated with a lower risk of eczema (odds ratio, 0.53; 95% CI, 0.34-0.83) at school age. Saliva contact via parental sucking of pacifiers was significantly associated with a lower risk of eczema (odds ratio, 0.24; 95% CI, 0.10-0.60) and allergic rhinitis (odds ratio, 0.33; 95% CI, 0.15-0.73), and had a borderline association with the risk of asthma in school-age children. Saliva contact during infancy may reduce the risk of developing eczema and allergic rhinitis in school-age children.

Pola Pengobatan Dan Analisis Potensi Interaksi Obat Pada Pasien Anak Demam Berdarah Dengue (Dbd) Di Rumah Sakit Kalimantan Timur

Background: Dengue hemorrhagic fever is caused by transmission of the dengue virus by the Aedes aegypti mosquito. Dengue hemorrhagic fever is common in tropical countries such as Indonesia, where new cases are found every year. According to WHO that dengue fever is the leading cause of hospitalization for children. Dengue hemorrhagic disease is more susceptible to infection in children because the child's immune system is still very weak, so the virus is easy to multiply quickly in the body. Knowing the pattern of treatment and analysis of potential drug interactions in pediatric patients with dengue hemorrhagic fever at the East Kalimantan Hospital. Methods: The research is non-experimental observational with a descriptive research design. The approach method used is the cross sectional method. Identification of data collection is carried out retrospectively, by taking sources from medical records for the years 2020-2021. Results: Of the 47 patient samples, the most cases were male patients as many as 27 patients (57%) with the age of 11 years as many as 11 patients (23%) with a length of stay of less than 7 days as many as 45 patients (96%). Based on the pattern of treatment, the most widely used therapy was the rehydration group (RL) 28 patients (60%). In the case of drug interactions occurred as many as 2 cases (3%). Conclusion: In this study show that there are cases of drug interactions.

THE DYNAMICS OF CHILD'S ORAL MICROBIOTA: A REVIEW

The oral microbiota in children changes with growth and development. The composition of the microbiota is influenced by genetic factors, oral environment, diet, and oral hygiene habits. Changes in oral microbiota composition have significant health implications in children, that is can contribute to the development of dental diseases, such as dental caries and periodontal disease. In addition, oral microbiota also plays an important role in the development of the child's immune system, therefore it is very important to understand the dynamics of child's oral microbiota. The method used in writing this scientific article is the literature review method. Various studies on oral microbiota in children show that in early life, oral microbiota is dominated by bacteria such as Streptococcus, Prevotella, and Veillonella. Along with the introduction of solid foods and the development of teeth, the composition of oral microbiota will change and become more diverse. Genetic factors can influence a child's susceptibility to the growth of pathogenic bacteria, while the oral environment, diet, and oral hygiene habits play an important role in the diversity and balance of oral microbiota. A balanced composition of microbiota plays a role in preventing dental diseases and maintaining overall oral health. Maintaining oral hygiene and adopting a healthy diet are important factors in maintaining the health of a child's oral microbiota. In addition, an understanding of the relationship between oral microbiota and a child's immune system is also an important factor in maintaining overall health.

Urgensi Aktifitas Bermain di Era Game Online untuk Kebugaran Jasmani Anak

The purpose of this writing work is for children to develop gross and fine motor skills, strengthen muscles and bones, improve balance, coordination, and flexibility of the body. In addition, playing can also increase heart and lung strength, and help boost a child's immune system. Play activities also provide important social experiences for children. Through play, children can learn to interact with others, share and work together in teams. This can help improve a child's social and emotional skills. In addition, play activities can also help reduce the risk of obesity in children. This writing method uses a review study method about the fitness condition of children during the online game period. Data analysis in this study used several literature reviews which were then analyzed qualitatively. The conclusion of this writing is that in the increasingly advanced technological era, children tend to spend more time in front of gadget screens and move less. By playing, children can reduce their screen time and increase physical activity. Overall, play activity is very important for the physical fitness and health of children. Parents and caregivers must provide opportunities and support for their children to play and move actively.&#x0D; Keywords: Children's Fitness, Play Activities, Physical Fitness

Assessment of the humoral immune response in children after immunization with different types of inactivated influenza vaccines in the 2019-2020 season

Causing millions of cases worldwide every year, influenza is one of the most common respiratory infections. The effectiveness of influenza vaccination and the nature of the resulting immune response may vary depending on the vaccine composition and age group. Since children are at the highest risk of disease and act as the main carriers of influenza, the assessment of the immunological efficacy of vaccines in this group is crucial for controlling the epidemic. Therefore, this study aimed to evaluate the characteristics of the humoral immune response in children after immunization with various types of inactivated influenza vaccines. An observational study was conducted in the 2019-2020 season and involved 230 children (&lt; 18 years old) and a comparison group of 87 adults aged 18 to 60 years. The subjects, who provided informed consent to participate, were vaccinated with one of three vaccines (Grippol Plus, Sovigripp, or Ultrix) in an open-label fashion. The humoral immune response was assessed by measuring the hemagglutination inhibition (HI) titer in the paired sera taken before and three weeks after vaccination. The immunogenicity of the vaccines in the age group under 18, met the CPMP criteria for the assessment of inactivated influenza vaccines in terms of the fold increase in antibody titers and the proportion of individuals with seroconversion to all three components (A/H1N1pdm09, A/H3N2, and B/Victoria). Although 6 to 18-year-old participants showed a more robust immune response to the B/Victoria component compared to the adult participants (aged 18 to 60), it was insufficient to ensure that 70% of the participants have a protective antibody titer. A comparative analysis of the vaccines’ immunogenicity was carried out for a subgroup of children aged 6-18 who had initially low antibody levels at the time of vaccination. The analysis showed that the split vaccine Ultrix outperformed the adjuvanted vaccine Grippol Plus in generating an antibody response to the component B/Victoria; however, the antibody responses to the A/H1N1pdm09 and A/H3N2 components did not differ between the two vaccines. The children under 6 years of age demonstrated a less pronounced humoral immune response to vaccination compared with the other age groups, which may be due to the age-related characteristics of the immune system in children of preschool age.

Experts’ Opinion on Immunity and Nutrition to Support Key Cognitive Functions

The interaction of micronutrients and macronutrients has been shown to have a significant impact on children's cognitive development. Furthermore, proper nutrition helps a child's immune system avoid infection and disease, which can impair nutrient absorption and lead to deficiency. Microbiotas in the gut play critical roles in body physiology, including nutrient absorption, infection resistance and immune system development. Furthermore, it is known that the gut microbiota influences immune cell maturation. Finally, these three factors are thought to influence neurogenesis and cognitive development. A virtual meeting was held with five invited experts to gain a better understanding of the relationship between nutritional factors, the immune system and cognitive development.

HUBUNGAN STATUS GIZI DENGAN KEJADIAN PNEUMONIA PADA ANAK DI KABUPATEN LOMBOK UTARA PROVINSI NUSA TENGGARA BARAT (NTB)

Pneumonia is a disease that attacks the lung tissue and is characterized by coughing and difficulty breathing which causes a wide spectrum of disease from mild to severe and deadly infections, depending on the causative pathogen. Nutritional status is a factor that is indirectly related to the incidence of pneumonia in children. Nutritional status has an important role in the child's immune system, malnutrition will be associated with a condition of decreased immune system so that various diseases can arise easily. The purpose of this study was to determine the relationship between nutritional status and the incidence of pneumonia in children in North Lombok District, NTB Province. The method used in this research is observational quantitative analytic research with a cross sectional study design. The sampling technique used consecutive sampling. The research was conducted at the District Hospital and all Community Health Centers in North Lombok Regency in April 2023. The research sample was 75 respondents. The data obtained were analyzed by Chi Square test. The significance value limit is (p≤ 0.05). The study showed that from 75 respondents, 57 people (76.0%) had positive pneumonia with malnutrition. The results of the bivariate analysis showed that the p - value &lt; 0.05 (p - value = 0.03). There is a significant relationship between nutritional status and the incidence of pneumonia in children in North Lombok, West Nusa Tenggara.

Childhood Vaccination Practices and Parental Hesitancy Barriers in Rural and Urban Primary Care Settings.

The purpose of our study was to identify primary care providers' (PCPs') practices in promoting childhood vaccination and their perceptions regarding barriers to vaccination in a primarily rural state. In January-May 2022, we conducted a mail and online survey of PCPs across Montana (n = 829). The survey included modules on routine immunizations in children 0-2 years old and COVID-19 vaccination in children 5-17 years old. The survey response rate was 36% (298/829). We categorized PCPs as working in rural (n = 218) or urban areas (n = 80), based on Rural-Urban Commuting Area codes. We then compared responses between rural and urban PCPs using chi-square tests. Urban PCPs (90-94%, depending on vaccine) stocked routinely recommended vaccines more frequently than rural PCPs (71-84%), but stocked the COVID-19 vaccine less often than rural PCPs (44% vs. 71%, respectively, p < 0.001). A higher percentage of rural providers reported parental beliefs that vaccine-preventable diseases are not severe enough to warrant vaccination (48% vs. 31%, p = 0.01) and concerns that vaccination will weaken their child's immune system (29% vs. 6%, p < 0.001). More rural (74%) compared to urban (59%) PCPs identified a social media campaign from local health departments promoting early childhood vaccinations as an effective strategy to increase childhood vaccination rates (p = 0.01). We identified key differences in some childhood vaccination practices and barriers between rural and urban PCPs. Interventions to increase rural vaccination rates could include increasing the number of providers stocking all recommended vaccines, identifying strategies to address parents' concerns regarding vaccine necessity, and collaborations with public health departments.

Changes in tension of immunity to pertussis by time of immunization

Objective: to identify the features of post-vaccination immunity to whooping cough, the state of immuno-logical reactivity in children of the Stavropol Territory. Material and methods. A comprehensive clinical and laboratory examination of 242 children aged 1 to 17 years was carried out. Serological examination of children was carried out at different times after revaccination, as well as an analysis of the parameters of the immune system in children of this age group. Results. A study of the level of immunity tension in children of early and school age showed that a low level of anti-pertussis antibodies was detected in 55% of children already 2-3 years after immunization, meaning that these children were at risk of pertussis, while protective titers of anti-pertussis antibodies were detected only in 34%, and high titers 11% of children. Conclusion. Children vaccinated in anamnesis in Stavropol Territory lose specific antibodies 3 years after the initial vaccination complex.

Review Paper on Characteristic Study of Water Sample for Public Schools and Colleges in Yavatmal

Abstract: This is a project of public interest and the child's immune system is particularly fragile following the corona phase. Water testing is so crucial, and this is the most crucial truth. why did we select this topic? We believe that the majority of the disease-causing bacteria are present in the water, which is the only reason we are testing the water sample. It is an environmentally friendly and crucial project for Yavatmal City because it is located in a hilly area and that most schoolchildren are no longer attending because of health concerns. Water is a valuable resource, and life on earth is impossible without it. Due to excessive and negligent use, water is becoming more and more polluted, and as a result, less and less of it is available for human use. Water pollution can be caused in a variety of ways, and both living things and non-living things are negatively impacted. In current situation water is a more contaminated that’s why it is verry essential to check the quality of water. The causes of water pollution include sewage systems, industrial waste, acid rain, the oil industry, aquatic plants, and household garbage. We studied environmental engineering in the previous section, when we looked at the water quality found in nature. It is well known; the quality of the water is not verified after a corona. As a result, we are undertaking this endeavour for public benefit.

HUBUNGAN ANTARA PENGETAHUAN, SIKAP, DAN PRAKTIK IBU DALAM PEMBERIAN MAKANAN PENDAMPING ASI (MPASI) PADA ANAK USIA 6-24 BULAN DENGAN KEJADIAN STUNTING DI WILAYAH KERJA PUSKESMAS SINGGAHAN, KABUPATEN TUBAN

Stunting is a chronic nutritional problem caused by many factors such as socioeconomic conditions, maternal nutrition during pregnancy, child morbidity, and child malnutrition. Complementary Foods can meet the nutritional needs of the baby's body which can no longer be supported by breast milk, as well as build the body's resistance and the development of the child's immune system against food and drink. The purpose of this study was to determine the relationship between knowledge, attitudes, and practices of mothers in giving complementary foods to children aged 6-24 months and the incidence of stunting in the working area of the Puskesmas Singgahan. This type of research is analytic observational with a cross-sectional design. The sampling technique used was proportionate simple random sampling. Samples were taken from as many as 38 respondents. Data were collected by conducting direct interviews using a questionnaire. Data analysis was performed in univariate, bivariate, and multivariate. The results showed that there was a relationship between the mother's knowledge about complementary foods p=0.030), the mother's attitude in giving complementary foods (p=0.019), the mother's practice in giving complementary foods (p=0.009) with the incidence of stunting. Based on the multivariate analysis, the variable of the mother's practice in giving complementary foods is the variable that has the most strong contribution to predicting stunting. The suggestion from this study is that mothers or child caregivers have to increase their knowledge and practice in giving complementary foods by frequently seeking information that is supported by an attractive stunting prevention program from the government.

Maternal provisions in type 1 diabetes: Evidence for both protective & pathogenic potential.

Maternal influences on the immune health and development of an infant begin in utero and continue well into the postnatal period, shaping and educating the child's maturing immune system. Two maternal provisions include early microbial colonizers to initiate microbiota establishment and the transfer of antibodies from mother to baby. Maternal antibodies are a result of a lifetime of antigenic experience, reflecting the infection history, health and environmental exposure of the mother. These same factors are strong influencers of the microbiota, inexorably linking the two. Together, these provisions help to educate the developing neonatal immune system and shape lymphocyte repertoires, establishing a role for external environmental influences even before birth. In the context of autoimmunity, the transfer of maternal autoantibodies has the potential to be harmful for the child, sometimes targeting tissues and cells with devastating consequences. Curiously, this does not seem to apply to maternal autoantibody transfer in type 1 diabetes (T1D). Moreover, despite the rising prevalence of the disease, little research has been conducted on the effects of maternal dysbiosis or antibody transfer from an affected mother to her offspring and thus their relevance to disease development in the offspring remains unclear. This review seeks to provide a thorough evaluation of the role of maternal microorganisms and antibodies within the context of T1D, exploring both their pathogenic and protective potential. Although a definitive understanding of their significance in infant T1D development remains elusive at present, we endeavor to present what has been learned with the goal of spurring further interest in this important and intriguing question.

Pijat Bayi Untuk Peningkatan Nafsu Makan Di Desa Oebola

Children under five years old (toddlers) are an age group that is vulnerable to nutrition and health. At this time, the child's immune system is not strong enough to catch infectious diseases. In addition, children also often have bad eating habits, namely children often do not want to eat or have a decreased appetite, causing their nutritional status to decrease and their growth and development to be disrupted. One effort that can be done to increase a child's appetite is to do baby massage. Currently massage techniques have been widely used for health and weight gain in babies. This community service method is counseling and demonstration. The community service sample is 10 mothers and toddlers. The location is in the village of Oebola, Fatuleu District, Kupang Regency. The instruments used in this community service are leaflets, and demonstrations of baby massage for each toddler. The activity was carried out on November 20 2022 at 08.00 WITA with 2 sessions namely counseling and demonstrations. The results of community service showed an increase in the knowledge of toddler mothers from the majority (50%) having sufficient knowledge to the majority (50%) having good knowledge. For children's appetite also experienced an increase from initially almost all (80%) had less appetite to almost all (90%) had a good appetite. The conclusion from this community service activity is that the provision of health education about infant massage can increase the knowledge of toddler mothers and infant massage can increase the appetite of children aged 0-5 years.&#x0D; &#x0D;

Identification of Parental Efforts in the Prevention of COVID-19 Infection in Children

COVID-19 is a global pandemic that has an impact on various aspects of life, one of which is the health of children. Children’s behavior in preventing transmission of COVID-19 needs to be controlled by parents. Reports of an increase in the number of infections in children, apart from the immune system of children who are still vulnerable, also need to be investigated for other causes, namely the efforts of parents to protect against transmission. Behavior and infection control efforts at home greatly affect the prevalence of virus transmission. The purpose of this study was to identify the efforts of parents in preventing COVID-19 infection in children in the New Normal era. This research was descriptive research. The population in this study were parents who had children aged 0-18 years. The sampling technique used was incidental sampling, and data analysis was done through frequency distribution. The results of the study found that the efforts of parents in preventing COVID-19 infection were mostly in the moderate category. This may be related to the level of parental education, the factor of domicile in the Malang Raya area, which has almost the same characteristics, the level of income, and the number of children. These three demographic factors have become a philosophy in efforts to prevent COVID-19 infection in children by parents.&#x0D; Keywords: infection, Covid-19, children, prevention efforts, parents, philosophy

Molecular Determinants of the Early Life Immune Response to COVID-19 Infection and Immunization.

Clinical manifestations from primary COVID infection in children are generally less severe as compared to adults, and severe pediatric cases occur predominantly in children with underlying medical conditions. However, despite the lower incidence of disease severity, the burden of COVID-19 in children is not negligible. Throughout the course of the pandemic, the case incidence in children has substantially increased, with estimated cumulative rates of SARS-CoV-2 infection and COVID-19 symptomatic illness in children comparable to those in adults. Vaccination is a key approach to enhance immunogenicity and protection against SARS-CoV-2. Although the immune system of children is functionally distinct from that of other age groups, vaccine development specific for the pediatric population has mostly been limited to dose-titration of formulations that were developed primarily for adults. In this review, we summarize the literature pertaining to age-specific differences in COVID-19 pathogenesis and clinical manifestation. In addition, we review molecular distinctions in how the early life immune system responds to infection and vaccination. Finally, we discuss recent advances in development of pediatric COVID-19 vaccines and provide future directions for basic and translational research in this area.

Progress in perioperative management of ABO-incompatible pediatric liver transplantation

ABO incompatible(ABO-I) liver grafts will affect the prognosis of liver transplantation. With the improvement of perioperative treatment,including plasma exchange,rituximab,splenectomy,etc.,the prognosis of ABO-I liver transplantation has been greatly improved. Because children's immune systems are not fully developed,the perioperative management of ABO-I pediatric liver transplantation is significantly different from that of adults. Reducing the perioperative anti-donor ABO antibody titer is the key to the perioperative management of ABO-I liver transplantation. This article summarizes literatures on the perioperative management of ABO-I pediatric liver transplantation, including the perioperative anti-rejection regimen in pediatric recipients of different ages, splenectomy, postoperative monitoring and postoperative complications, etc.

Features of the pathology of the lymphoid ring of the pharynx in children during the COVID-19 pandemic

Introduction. For many years, one of the problems in medicine that attracted the attention of specialists from different areas of medicine was herpes virus infection. There are many reasons for this, including the often persistent nature of the course, a wide variety of clinical manifestations and routes of transmission, and many target organs of this infection. The disease can proceed in different ways, it has both asymptomatic, mild and rather severe course. For otorhinolaryngologists, Epstein-Barr virus (EBV), cytomegalovirus (CMV) and herpes virus 6 (HHV-6) are the most relevant and important in the context of the treated site of the pathological process. Since the end of 2020, humanity has faced a new, previously unexplored viral infection – a new coronavirus infection. And just like the previously studied herpes virus infection, COVID-19 has a large number of clinical manifestations and affects a huge number of organs and organ systems.Aim. To determine the types of interplay between COVID-19 and herpesviruses (EBV, CMV, HHV-6) in children of different ages with abnormalities of the lymphoid ring of pharynx.Materials and methods. In the course of the study, a comprehensive examination was carried out and the results of examination of children with pathology of the lymphoid ring of the pharynx who had herpes virus infection and COVID-19 were analyzed.Results. The results of the study obtained in the course of the work are consistent with the data that the virus carrier. The presence of an infectious agent triggers the production of macrophages and leads to clonal selection of T and B cells. Further, lymphocytes are transformed into lymphoblasts, which are capable of division and their transformation into plasma and B-memory cells occurs. As a result of this process, hypertrophy of the lymphoid tissue occurs and its reactive activity increases. (persistent herpesvirus infection) is one of the important causes of lymphoid proliferation in children. The combination of antigenic load and immaturity of the immune system in children is accompanied by an increase in the activity of lymphoid tissue and its compensatory activity.Conclusions. Past mild and asymptomatic COVID-19 can contribute to the reactivation of lymphotropic herpesviruses, first of all EBV, in 55-65% of children of preschool and school age.