Objectives The purpose of this study is to longitudinally examine the changes in the life satisfaction of adolescents from multicultural families and the mother's acculturation stress according to the change of time, and the change in the relationship between adolescents' life satisfaction and mother's daily life stress. Methods For this purpose, data from the 4th and 6th years (2014-2016) were used among the multicultural youth panel survey data of the Korea Youth Policy Institute. The subjects of this study were 1,625 mothers from multicultural families and 1,635 adolescent children from 1st to 3rd grade of middle school. Results The research results are as follows. First, it was found that the life satisfaction of adolescents from multicultural families and the mother's acculturation stress gradually decreased as the children progressed from the first to the third year of middle school, and the mother's daily life stress gradually increased. Second, the higher the life satisfaction of children of multicultural families during the first year of middle school, the lower the daily life stress and acculturation stress of foreign mothers measured at the same time. In addition, the rate of change in life satisfaction among adolescents from multicultural families during the first to third year of middle school had a negative effect on the rate of change in mother's daily life stress. Third, it was found that the mother's initial value of acculturation stress plays a partial mediating role in the relationship between the initial value of life satisfaction of adolescents from multicultural families, and the mother's initial value of daily life stress and the rate of change of daily life stress. Conclusions The results of this study have policy implications that social measures should be devised to enable multicultural families to settle healthily in Korean society.