Chickpea Genotypes Research Articles

Arbuscular mycorrhizae reduced arsenic induced oxidative stress by coordinating nutrient uptake and proline-glutathione levels in Cicer arietinum L. (chickpea).

Accumulation of Arsenic (As) generates oxidative stress by reducing nutrients availability in plants. Arbuscular mycorrhizal (AM) symbiosis can impart metalloid tolerance in plants by enhancing the synthesis of sulfur (S)-rich peptides (glutathione- GSH) and low-molecular-weight nitrogenous (N) osmolytes (proline- Pro). The present study, therefore investigated the efficiency of 3 AM fungal species (Rhizoglomus intraradices-Ri, Funneliformis mosseae -Fm and Claroideoglomus claroideum- Cc) in imparting As (arsenate-AsV -40 at 60 mg kg-1 and arsenite- AsIII at 5 and 10 mg kg-1) tolerance in two Cicer arietinum (chickpea) genotypes (HC 3 and C 235). As induced significantly higher negative impacts in roots than shoots, which was in accordance with proportionately higher reactive oxygen species (ROS) in the former, with AsIII more toxic than AsV. Mycorrhizal symbiosis overcame oxidative stress by providing the host plants with necessary nutrients (P, N, and S) through enhanced microbial enzyme activities (MEAs) in soil, which increased the synthesis of Pro and GSH and established a redox balance in the two genotypes. This coordination between nutrient status, Pro-GSH levels, and antioxidant defense was stronger in HC 3 than C 235 due to its higher responsiveness to the three AM species. However, Ri was most beneficial in inducing redox homeostasis, followed by Fm and Cc, since the Cicer arietinum-Ri combination displayed the maximum ability to boost antioxidant defense mechanisms and establish a coordination with Pro synthesis. Thus, the results highlighted the importance of selecting specific chickpea genotypes having an ability to establish effective mycorrhizal symbiosis for imparting As stress tolerance.

Effect of Seed Biopriming on Germination & Seedling Attributes in Chickpea (Cicer arietinum L.)

The Study was conducted during Zaid season of 2022-2023 with IPC-05-59 genotype of chickpea (Cicer arietinum L.) Find out the Effect of seed biopriming on germination & seedling attributes in chickpea (Cicer arietinum L). The design of experiment was Completely Randomized Design comprising treatments [viz.T0-uninoculatedT1-Bacillus sp, T2- Mesorhizobium sp., T3- Pseudomonas sp, T4- Bacillus sp(0.5)+ Mesorhizobium sp.(0.5),T5- Pseudomonas sp.(0.5)+ Mesorhizobium sp.(0.5), T6- Bacillus sp.(0.5)+Pseudomonas sp(0.5).+ Mesorhizobium sp.(0.5), T7- Bacillus sp,T8- Mesorhizobium sp, T9- Pseudomonas sp, T10- Bacillus sp.(0.5) + Mesorhizobium sp(0.5),T11-Pseudomonas sp. (0.5)+ Mesorhizobiumsp(0.5),T12- Bacillus sp.(0.5) + Pseudomonas sp. (0.5) + Mesorhizobium sp. (0.5) . The treated seeds along with control were evaluated for their morpho-physiological, growth, yield, root parameters and biochemical parameters under laboratory conditions, The study revealed that seeds biopriming techniques with Bacillus sp.(0.5)+ Pseudomonas sp(0.5).+ Mesorhizobium sp.(0.5) concentration @ 1.0ml for 1 hours T6 recorded significantly higher germination percent (93%), rate of germination (5.38), mean germination time (11.16), root length (11.73cm),shoot length(13.27cm),seedling length(25.00cm), fresh weight (7.91gm),dry weight (1.62gm), vigor index-I (2324.91), vigor index – II (135.82), seed density (1.27), electrical conductivity (0.817), seed metabolic efficiency (1.018). which was followed by seedprimingwithT12- Bacillus sp (0.5) +Pseudomonas sp (0.5) +Mesorhizobium sp (0.5) @ 1.5ml for 2 hours and minimum was recorded in T0 (control).

Evaluation of chickpea genotypes in Cerrado conditions in the South of the state of Tocantins

The chickpeas (Cicer arietinum L.) has potential for cultivation in the Brazilian cerrado region, its sowing supplies both the domestic market and exports, bringing benefits to the economy. In this context, the objective of this study was to evaluate morphophysiological and yield parameters in six genotypes of chickpeas with different genetic basis, which make it possible to select materials suitable for commercial cultivation under the conditions of the cerrado in the south of the state of Tocantins. Six treatments were used to carry out the experiment, each one characterized by one of the following: BRS Cícero; BRS Aleppo; BRS Khalifa; BRS Cristalino; Jamu 96 and BRS Toro. To define the best genotypes, morphophysiological characteristics of the crop were evaluated, namely: grain yield, weight of a thousand grains, plant height, number of days until flowering, number of days until maturation, chlorophyll a and b index, internal concentration of CO2, photosynthesis, stomatal conductance and transpiration. The cultivation of chickpeas in the cerrado is viable for the region. The Jamu 96 genotype stands out among the others, in the main morphophysiological characteristics, being the one that obtained the highest production averages. The genotypes BRS Cícero, BRS Aleppo and BRS Khalifa showed potential for improvement in production, due to their thousand-grain weight being similar to Jamu 96, making evident the need for genetic improvement in order to obtain genotypes more adapted to the soil and climate conditions of southern Brazil. Tocantins, making them more adapted to the region and thus increasing the production potential.

Evaluation of Desi Chickpea (Cicer arientinum L.) Landraces for Heat Tolerance using Morpho-phenological Traits and Stress Tolerance Indices (STI)

Background: Chickpea is a globally important commercial crop and a key source of protein for vegetarian populations. Though chickpea was domesticated at least 3000 years ago, research into abiotic stress tolerance has been limited compared to cereals. Methods: An experiment was conducted with a set of seventy-one genotypes of Desi chickpea (Cicer arietinum L.) to isolate high temperature tolerant genotypes using randomized block design (RBD) under two different sowing conditions (normal and late/terminal heat stress) and two growing seasons (rabi-2017-18 and 2018-19) at the Pulses Research Station of Junagadh Agricultural University. The screening of heat tolerant genotypes was done based on nine morpho-phenological traits, stress tolerance indices and correlation amongst them. Result: The heat tolerant genotypes of chickpea had less percentage of reduction in numbers of pods, seed yield per plant and 100-seeds weight. Five genotypes viz., ICC 4958, ICC 14595, ICC 8318, GG 4 and ICCV 92944 were identified as highly heat tolerant on the basis of HSI and YSR. These tolerant genotypes and screening criteria’s can be of great importance to identify donor parents to develop new cultivars for the farmers growing chickpea in the heat prone regions.

Shoot transcriptome revealed widespread differential expression and potential molecular mechanisms of chickpea (Cicer arietinum L.) against Fusarium wilt.

The yield of chickpea is severely hampered by infection wilt caused by several races of Fusarium oxysporum f. sp. ciceris (Foc). To understand the underlying molecular mechanisms of resistance against Foc4 Fusarium wilt, RNA sequencing-based shoot transcriptome data of two contrasting chickpea genotypes, namely KWR 108 (resistant) and GL 13001 (susceptible), were generated and analyzed. The shoot transcriptome data showed 1,103 and 1,221 significant DEGs in chickpea genotypes KWR 108 and GL 13001, respectively. Among these, 495 and 608 genes were significantly down and up-regulated in genotypes KWR 108, and 427 and 794 genes were significantly down and up-regulated in genotype GL 13001. The gene ontology (GO) analysis of significant DEGs was performed and the GO of the top 50 DEGs in two contrasting chickpea genotypes showed the highest cellular components as membrane and nucleus, and molecular functions including nucleotide binding, metal ion binding, transferase, kinase, and oxidoreductase activity involved in biological processes such as phosphorylation, oxidation-reduction, cell redox homeostasis process, and DNA repair. Compared to the susceptible genotype which showed significant up-regulation of genes involved in processes like DNA repair, the significantly up-regulated DEGs of the resistant genotypes were involved in processes like energy metabolism and environmental adaptation, particularly host-pathogen interaction. This indicates an efficient utilization of environmental adaptation pathways, energy homeostasis, and stable DNA molecules as the strategy to cope with Fusarium wilt infection in chickpea. The findings of the study will be useful in targeting the genes in designing gene-based markers for association mapping with the traits of interest in chickpea under Fusarium wilt which could be efficiently utilized in marker-assisted breeding of chickpea, particularly against Foc4 Fusarium wilt.

Differential expression of antimicrobial metabolites, phenylpropanoid and phytohormone metabolic pathway genes determines resistance or susceptibility to Ascochyta rabiei in chickpea

AbstractBlight caused by Ascochyta rabiei is a major constraint in the productivity of chickpea (Cicer arietinum). The mechanisms governing resistance/susceptibility to blight in chickpea are poorly understood. We used a blight‐resistant (HC1) and a blight‐susceptible (GPF2) genotype of chickpea and genes of pathogenesis‐related proteins (PRPs), phenylpropanoid pathway metabolites, abscisic acid (ABA), gibberellic acid (GA) and jasmonic acid (JA) to understand the role of these in A. rabiei resistance/susceptibility. The JA, ABA and GA biosynthesis genes of chickpea were retrieved, characterized and gene‐specific primers were used for transcriptional studies. Gene expression revealed that chickpea activated its defences rather quickly and well before initiation of spore germination. In resistant HC1, the majority of the JA, GA and phenylpropanoid pathway genes had peak maxima at 2 h post‐inoculation (hpi) whereas PRPs/defence genes had peak maxima at 24/36 hpi implying that defence to A. rabiei in chickpea is composed of a two‐tier system separated by time: immediately after spore attachment and at or just prior to host penetration. Unlike HC1, susceptible GPF2 was late in activation of defence responses or did not activate them. Another striking difference between HC1 and GPF2 was up‐regulation of ABA biosynthesis genes in inoculated GPF2 and down‐regulation in HC1. This study revealed that phenylpropanoids, PRPs, JA, 8‐(1R,2R)‐3‐oxo‐2‐(Z)‐pent‐2‐enyl cyclopentyl octanoate, (15Z)‐12‐oxophyto‐10,15‐dienoic acid and methyl‐jasmonate govern resistance to A. rabiei in chickpea whereas ABA governs susceptibility.

Unveiling the genetic basis of Fusarium wilt resistance in chickpea using GWAS analysis and characterization of candidate genes.

Introduction: Chickpea is a legume crop that thrives in regions with semi-arid or temperate climates. Its seeds are an excellent source of proteins, carbohydrates, and minerals, especially high-quality proteins. Chickpea cultivation faces several challenges including Fusarium wilt (FW), a major fungal disease that significantly reduces productivity. Methods: In this study, a Genome-wide Association Analysis (GWAS) was conducted to identify multiple genomic loci associated with FW resistance in chickpea. We conducted a comprehensive evaluation of 180 chickpea genotypes for FW resistance across three distinct locations (Ethiopia, Tunisia, and Lebanon) during the 2-year span from 2015 to 2016. Disease infection measurements were recorded, and the wilt incidence of each genotype was calculated. We employed a set of 11,979 single nucleotide polymorphisms (SNPs) markers distributed across the entire chickpea genome for SNP genotyping. Population structure analysis was conducted to determine the genetic structure of the genotypes. Results and Discussion: The population structure unveiled that the analyzed chickpea germplasm could be categorized into four sub-populations. Notably, these sub-populations displayed diverse geographic origins. The GWAS identified 11 SNPs associated with FW resistance, dispersed across the genome. Certain SNPs were consistent across trials, while others were specific to particular environments. Chromosome CA2 harbored five SNP markers, CA5 featured two, and CA4, CA6, CA7, and CA8 each had one representative marker. Four SNPs demonstrated an association with FW resistance, consistently observed across a minimum of three distinct environments. These SNPs included SNP5826041, SNP5825086, SNP11063413, SNP5825195, which located in CaFeSOD, CaS13like, CaNTAQ1, and CaAARS genes, respectively. Further investigations were conducted to gain insights into the functions of these genes and their role in FW resistance. This progress holds promise for reducing the negative impact of the disease on chickpea production.

Correlation studies for seed yield and its components in chickpea (Cicer arientinum L.) germplasm

The association between seed yield and yield contributing characters was studied by using 41 chickpea genotypes. The characters viz. number of pods per plant, 100 seed weight, harvest index, number of secondary branches per plant and plant height recorded a highly positive significant correlation with seed yield at both genotypic and phenotypic levels. This indicates the simultaneous improvement of these characters through selection.

Characterization of Spanish chickpea genotypes (Cicer arietinum L.): proximate, mineral, and phenolic compounds composition

Characterization of Spanish chickpea genotypes (Cicer arietinum L.): proximate, mineral, and phenolic compounds composition

Optimizing Chickpea Yield: GGE Biplot Analysis of Sowing Seasons

Maximizing chickpea production in varying environmental conditions is crucial for ensuring food security and agricultural sustainability. This study addresses this challenge by evaluating sixty-six chickpea genotypes across winter and spring sowing seasons over two years (2016-17 and 2017-18). Utilizing an Augmented Trial Design with three replications and six check varieties, the study reveals significant effects of genotype, sowing season, and their interaction on all examined traits (p≤0.01). Notably, winter sowing season led to a substantial increase in yield, demonstrating up to a 57.1% improvement compared to spring sowing season. The first two components of principal component analysis accounted for 67% of the total variation of chickpea genotypes. In the examined traits, the lowest variation was observed in 100 seed weight, followed by seed yield. Biplot analysis, Pearson’s correlation, and heatmap analysis indicated a positive association of seed yield with pod number of plant and seed number per plant, yet a negative association for plant height, first pod height and 100 seed weight. SMN56, SMN57, and SMN51 genotypes consistently performed well across both sowing and growing seasons for desired traits. Furthermore, genotypes SMN13, SMN20, SMN37, SMN38, and SMN54 excelled during spring sowing season, while Gülümser, İnci, Aydın 92, SMN14, SMN20, and SMN39 were superior in winter. This study not only underscores the advantage of winter sowing for improved yield and other traits but also demonstrates the effectiveness of GGE biplot analysis in genotype selection, offering valuable implications for future chickpea breeding efforts.

Effect of salinity stress on Morphophysiological traits in chickpea genotypes

Effect of salinity stress on Morphophysiological traits in chickpea genotypes

Study of yield and biochemical parameters of newly introduced chickpea (Cicer arietinum L.) genotypes and use of valuable forms in breeding

Study of yield and biochemical parameters of newly introduced chickpea (Cicer arietinum L.) genotypes and use of valuable forms in breeding

Morphological and biochemical characterization of some chickpea (Cicer arietinum L.) genotypes in Bangladesh

Morphological and biochemical characterization of some chickpea (Cicer arietinum L.) genotypes in Bangladesh

Field screening of chickpea genotypes for locating resistance sources against pod borer Helicoverpa armigera (Hübner)

Field screening of chickpea genotypes for locating resistance sources against pod borer Helicoverpa armigera (Hübner)

Genetic Diversity and Inter-Relationships of Morpho-Agronomic Traits in Kabuli Chickpea Genotypes under Autumn Sowing Condition

Genetic Diversity and Inter-Relationships of Morpho-Agronomic Traits in Kabuli Chickpea Genotypes under Autumn Sowing Condition

Genetic variability and stability of Desi chickpea (Cicer arietinum L.) genotypes under late sown terminal heat stress conditions

Genetic variability and stability of Desi chickpea (Cicer arietinum L.) genotypes under late sown terminal heat stress conditions

Identifying Genetic Linkage Groups and Markers for Leaf Miner Resistance in Chickpea through QTL Analysis and Field Validation

Chickpea is a commonly grown crop, but it is vulnerable to biotic and abiotic stresses. Leaf miner (Liriomyza cicerina) is a pest that can cause severe yield losses of up to 40% if not properly controlled. This study was conducted at ICARDA (Aleppo, Syria) during the 20112012 growing seasons. Two recombinant inbred lines, ILC 5901 (LM resistant) and ILC 3397 (LM susceptible), were crossed to yield 350 F2 plants, which were then screened for pathogen tolerance. The resistance of the plants was screened using a scale of one to nine, with 1 indicating complete resistance and 9 indicating complete susceptibility. A set of 600 simple sequence repeat (SSR) markers were validated on both parents, and 51 of these markers showed variation and were used to construct a genetic linkage map. QTL analysis was performed to determine the linkage groups responsible for line variations. The QTL analysis found that linkage groups TA37, TA34, and H4F03 were responsible for 22% of line variations, while unmapped NCPRG48 and H1C092 revealed 55.3% and 26.8% of the LIS variance, respectively, and displayed a warped dominance toward the susceptible parent. The H1C092 marker, which is significantly associated with LM, is located on Chr3 near a gene encoding the glutathione S-transferase gene family enzyme, which protects cellular macromolecules from attack by reactive electrophiles. The highly associated markers were field tested for three years to confirm their connection with LM resistance in 200 chickpea genotypes. The study showed marker-associated selection, which could accelerate the conventional breeding of LM-resistant chickpea germplasm. The markers linked to LM resistance and the identification of the protective enzyme gene offer promising avenues for further research. This study represents a significant step forward in understanding the genetics of LM resistance in chickpea and provides valuable information for breeding programs aimed at improving chickpea production.

Unclenching the potentials of global core germplasm for root nodulation traits for increased biological nitrogen fixation and productivity in chickpea (Cicer arietinum L.)

Chickpea being an important food legume crop is able to fix the atmospheric nitrogen and form root nodules that support biologicalnitrogen fixation- a sustainable alternative for nitrogen supply to agriculture worldwide. In order to support findings on nodule formation in chickpeas, a diverse core set of 300 chickpea genotypes, including four checks, was evaluated for morphological and nodulation traits in four different environments. The maximum genotypic and phenotypic coefficient of variation was observed for a number of nodules, nodule fresh weight, shoot fresh weight, and number of seeds per plant. The heritability for most of the characters ranged from 26.56 for days to 50% flowering to 99.61 for a number of pods per plant. The genotypic and phenotypic correlation coefficient analysis revealed that the number of nodules was positively correlated with seed yield. Further, seed yield was partitioned into direct and indirect effects at genotypic and phenotypic levels through path coefficient analysis. Significant genotypic correlations and direct positive effects were exhibited by a number of nodules and number of pods per plant on grain yield. Based on the D2 statistics, the number of seeds per plant, number of pods per plant, shoot fresh weight and number of nodules have been identified as top-ranking yield contributing traits. The genotypes ICC1013 and ICC16569 for a number of nodules and ICC1049 for the trait number of seeds per plant were identified as potential stable donors for the respective traits with high mean yield, heritability and genetic advance across the locations and can be used as donors in the chickpea breeding programs for increasing biological nitrogen fixation and enhancement of the crop productivity.

Disease Surveillance and Pathogenic Variability of Ascochyta Rabiei causing Blight of Chickpea in Punjab Pakistan

Chickpea is an important legume crop and plays a significant role in global food security. Production is affected by many biotic and abiotic factors. The most significant biotic factor, Ascochyta rabiei is a fungal pathogen that causes Ascochyta blight in chickpeas, leading to substantial yield losses globally and is the primary cause in Pakistan. This pathogen causes heavy yield losses annually in the chickpea industry. The knowledge of the pathogenic variability of A. rabiei isolates can significantly impact the effectiveness of blight disease management strategies. A survey was conducted to collect blight samples to assess the pathogenic variability of A. rabiei isolates from different chickpea-growing regions of Punjab. The disease incidence and severity data were recorded using the quadrat square method in the fields. After isolation and purification, the pathogenicity test was conducted for conformation of the isolates. The virulence of ten isolates of A. rabiei was analyzed on six differential chickpea genotypes under artificial inoculation conditions in the glasshouse. The differential genotypes showed different levels of resistance and susceptibility to the isolates. Based on the reactions, the population of A. rabiei was grouped into different groups using a 1-9 rating scale. Only two isolates, BKR-01 and LAY-01, produced a high virulent effect against six germplasm. These isolates were collected from the Bhakkar and Layyah. The isolate MTN-1 were categorised as the least virulent. At the same time, the remaining isolates showed a moderate level of reaction.

Genetic Characterization and Variability Analysis of Chickpea (Cicer arietinum) Elite Germplasm under Different Cropping Conditions

In current study 113 diverse genotypes of chickpea has been evaluated during rabi 2019-20 and 2020-21 under timely sown (TS), late sown (LS) and very late sown (VLS) cropping environment in augmented block design at research farm of ICAR-IIPR, Kanpur for genetic characterization to access the presence of variability among the major grain yield attributing traits under changing cropping conditions. Variation due to block were insignificant and error variance was significant for all three different trials (ie., TS, LS and VLS) conducted during rabi 2019-20 and 2020-21(Table 5) In the present study highest genotypic and phenotypic coefficient of variation was observed for UFP, SYP (g), PB, Y (kg/ha), PY (g), HI (%), BMP (g), HSW (g) and FP (Table 5) While the traits viz., DFI, DFF, DPI, DFP, DMI, DM and PHT (cm), BY (g) and NSP exhibited the moderate to low range of GVC and PCV value under TS, LS and VLS cropping conditions During rabi 2019-20 and 2020-21. Highest value of heritability (%) >60% have been observe for the traits DFI, DFF, DMI, DM, PB, Y (kg/ha), HSW (g), SYP (g), BMP (g) and FP in all three different cropping environments (Table 6 in the current study correlation coefficient analysis have been estimated for the Correlation values (Table 6) for all three different trials viz Timely sown (TS), Late sown (LS) and Very Late sown (VLS) conducted in Rabi 2019-20 and 2020-2021. The Pearson correlation coefficients of pooled data were calculated for Eighteen morphological traits The major yield contributing traits such as DFF, PHT (cm), PB, SYP (g), PY (g), Y (g), HSW (g), BY (g) and HI (%) have significantly correlated with all the traits except UFP and NSP (Table 6) The PY (g) exhibited strong positive correlation with Y (Kg/ha) (0.857** and 0.964**); HSW (g) (0.544* and 0.412*); BY (g) (517* and 0.856**); SYP (g) (0.628**and 0.506*); BMP (g) (0.553* and 0.494*); HI (%) (0.459* and 0.706**). Similarly, another chief yield contributing traits like SPY (g) is also positively correlated BMP (g) (0.536* and 0.682**); HI (%) (0.678** and 0.779**); FP (0.774** and 0.964**) and NSP (0.456* and 0.503*) except UFP (Table 7 maximum percentage of variance for all 113 diverse chickpea genotypes has been recorded for PC1(26.83, 26.63 & 31.46 in 2019-20; 29.87, 35.15 & 30.2 in 2020-21) and PC2 (24.19, 22.81 & 12.66 in 2019-20; 18.96, 15.43 & 17.4 in 2020-21) for all three separate trials i.e., TS, LS and VLS (Table 7).