Chicken Offal Research Articles

Investigating the Energy Potential and Degradation Kinetics of Nine Organic Substrates: Promulgating Sustainability in Developing Economies

To standardize, systematize, and improve the efficiency of the evaluation of biodegradable materials for large-scale biogas projects to support clean and sustainable energy development in emerging economies from a sub-Saharan African perspective, this paper analyzes and fits the potential for methane production (biochemical methane potential, BMP) and degradation kinetics of materials based on the gas production and degradation dynamics obtained from methane potential experiments. The first-order, modified first-order, and Gompertz models are used for analysis and fitting. The Gompertz model shows higher accuracy in fitting the methane production potential curve of screened materials, and the fitted methane potential values are close to the experimental values. When using BMP1% (cumulative gas production reaching 1% of cumulative gas production per day) as a quantitative indicator for the methane production potential of materials, the cumulative methane production reaches over 85% of the cumulative methane production at the end of the experiment. The BMP test time is shortened by 26.98% to 72.06%. Among the screened materials, the methane production potential (calculated using BMP1%) of dry rice straw, maize leaves, fresh rice, soybean straw, maize stalks, chicken manure hydrolysate, chicken feathers, kitchen/food waste, and chicken offal are 234.14, 241.01, 253.34, 331.40, 305.80, 508.41, 510.10, 630.7, and 621.32 mL/g, respectively. The kinetic parameters show that among the nine materials, cellulose materials (except for maize stalks and soybean straw), chicken manure, and kitchen waste are easily degradable materials. In contrast, chicken feathers and offal are slowly degradable materials. The study posits that comparing standardized methane production potential and methane production kinetic parameters among materials improves the efficiency of screening materials and is critical for biogas projects.

Comprehensive analysis of Salmonella in poultry meat and products in Türkiye: Prevalence, antibiotic susceptibility and genomic characterisation

SummaryThe study aimed to determine the prevalence and characteristics of Salmonella isolated from raw chicken meat and products. For this purpose, a total of 293 samples were collected, including chicken breast (n = 90), skinned drumstick (n = 80), skinned chicken chop (n = 42), wing (n = 32), chicken offal (n = 27) and chicken patty (n = 22). The samples were subjected to Salmonella enterica. detection and the obtained suspicious isolates were confirmed by conventional PCR. Their phenotypical antibiotic resistance profiles were subsequently determined. The prevalence of Salmonella Enteritidis and Typhimurium serovars among S. enterica isolates were investigated using TaqMan probe Real‐Time PCR (qPCR) analysis, and the detected serovars were evaluated with whole genome sequencing. In the study, 112 (38.22%) of the 293 chicken samples contained S. enterica, with five (4.46%) and one (0.89%) of the isolates identified as Salmonella Enteritidis and Typhimurium, respectively. Antibiotic resistance analysis revealed that all isolates were sensitive to Meropenem and Aztreonam, while the most resistant antibiotics were Doxycycline (96.42%) and Trimethoprim‐sulfamethoxazole (71.42%). Whole genome sequencing, specifically SNP‐based phylogenetic analyses, indicated that Salmonella Enteritidis and Typhimurium isolates were distinct clones. All Salmonella Enteritidis isolates shared the same antigenic profiles (9: g, m:‐) and cgMLST types of 11, while the Salmonella Typhimurium isolate had cgMLST type 19 and a 4:i:1,2 antigenic profile. It was observed that the phenotypic resistance profiles of the isolates were consistent with the whole genome characterisation. The data obtained in the study reveal the continued importance of Salmonella monitoring for the poultry industry across different regions of Türkiye to maintain food safety. Chicken meat and products are indispensable to public health in providing healthy nutrition and access to animal protein. The microbiological and epidemiological risks observed in mass production can be minimised, particularly by integrating epidemiological and molecular findings with an effective strategy.

Characterization of Campylobacter jejuni and Campylobacter coli isolates from chicken offal in Metro Manila, Philippines: Insights from virulence gene prevalence and multilocus sequence typing analysis.

Campylobacteriosis is a human infection primarily caused by Campylobacter jejuni and Campylobacter coli. Consumption of contaminated chicken and poultry products is the main mode of transmission. These bacteria possess virulence factors, including adhesins and toxins, which contribute to their pathogenesis. Moreover, their large genomes undergo frequent genetic recombination, resulting in a high degree of genetic diversity. However, limited information is available regarding the virulence and genotypic diversity profiles of these microorganisms in the Philippines. The objective of this study was to address this knowledge gap by characterizing Campylobacter isolates obtained from chicken offal sold in wet markets in Metro Manila, Philippines. Multilocus Sequence Typing (MLST) analysis was performed to determine the sequence types, resulting in the identification of 13 unique sequence types, including nine previously unreported ones, and three clonal complexes. Notably, the widespread sequence type ST-305 was found in samples from different markets. Furthermore, six isolates deposited in the Campylobacter PubMLST database were identified as C. coli based on allele profiles. Profiling using 10 selected virulence genes revealed that more than half of the isolates carried these genes. The most prevalent virulence gene was cadF (100%), followed by flaA (95%), racR, cdtA, cdtB, and cdtC (85%). The genes dnaJ and ceuE were also present in 75% of the isolates. Despite the limited sample size, the findings of this study reveal a significant level of genotypic diversity among the Campylobacter isolates. This diversity has important implications for source attribution studies and the identification of strains involved in campylobacteriosis outbreaks. Furthermore, the investigation of virulence factors associated with colonization and invasion of the avian gut can provide insights for the development of practical applications in Campylobacter control strategies. Understanding and addressing these factors are crucial steps toward mitigating the risk of Campylobacter infections and enhancing public health efforts.

Prevalence and Antimicrobial Resistance of Campylobacter jejuni/coli and Analysis of Macrolide Resistance Isolates from Retail Meat in Tokyo, Japan (2010-2019)

This study aimed to investigate the prevalence and antimicrobial sensitivity of Campylobacter jejuni and Campylobacter coli in retail meat (chicken, beef, pork, venison, wild boar, horse, lamb and mutton) in Tokyo (Japan) from 2010 to 2019. Furthermore, the resistance mechanism of erythromycin (EM)-resistant strains was analysed. C. jejuni had a highly positive rate in domestic chicken meat (53.4%, 334/626 samples), domestic chicken offal (49.3%, 34/69 samples), and domestic beef offal (28.3%, 47/166 samples), while C. coli had a high positivity rate in domestic pork offal (31.7%, 44/139 samples). The positive rate of C. jejuni was significantly higher in offal than that in meat in domestic beef, while the positive rate of C. coli was significantly higher in offal than that in meat in domestic beef and domestic pork (p<0.05). In the isolates, 1.0% (6/631 strains) of C. jejuni and 36.2% (55/152 strains) of C. coli were EM resistant, with 41.5% (262/631 strains) of C. jejuni and 65.1% (99/152 strains) of C. coli being ciprofloxacin resistant. A2075G mutation of the 23S rRNA gene was confirmed in all EM-resistant strains.

Haematological responses of replacement levels of fish meal with chicken offal in catfish (Clarias gariepinus) diets

Fish demand by consumers keeps increasing, yet constraints to the production of fish are encountered, the commonest of which is the high cost and difficulty in acquiring fish feeds in Nigeria. The objective of this study was to determine the effect of the formulated test diets of 40% protein concentrate using varying levels of chicken offal diets on the haematological parameters of the African Catfish (Clarias gariepinus). The chicken offal was used to replace fish meal at inclusion levels of 0% (control, treatment A), 25% (treatment B), 50% (treatment C), 75% (treatment D) and 100% (treatment E) respectively. Three hundred (300) fingerlings of average length, 8.12±0.48 cm, and average weight, 3.68±0.00 g, were stocked in concrete tanks. Fish were fed at 5 per cent body weight for 8 months. Physicochemical parameters including; water temperature (°C), pH, dissolved oxygen and ammonia were monitored every four days and maintained within acceptable ranges for fish culture. Data obtained were analysed using analysis of variance (ANOVA). Results showed haemoglobin (Hb), Packed cell volume (PCV), Mean corpuscular haemoglobin concentration (MCHC) and mean corpuscular volume (MCV) to be statistically different (ANOVA, p&lt;0.05) between the treatments, although no significant difference (ANOVA, p&gt;0.05) was observed in white blood cell (WBC), Mean Corpuscular haemoglobin (MCH) and erythrocyte sedimentation rate (ESR). Haematological parameters results were within the ranges for fish well-being. Chicken offal were suitable as partial replacement at (25, 50, 75 and 100%) for fish meal, it enhanced the hematological performance of C. gariepinus due to its mineral components.

Virulence and Antimicrobial Resistance Profiling of Salmonella Serovars Recovered from Retail Poultry Offal in KwaZulu-Natal Province, South Africa.

As poultry organ meat is widely consumed, especially in low- and middle-income countries, there is reason to investigate it as a source of Salmonella infections in humans. Consequently, the aim of this study was to determine the prevalence, serotypes, virulence factors and antimicrobial resistance of Salmonella isolated from chicken offal from retail outlets in KwaZulu-Natal, South Africa. Samples (n = 446) were cultured for the detection of Salmonella using ISO 6579-1:2017. Presumptive Salmonella were confirmed using matrix-assisted laser desorption ionisation time-of-flight mass spectrometry. Salmonella isolates were serotyped using the Kauffmann-White-Le Minor scheme and antimicrobial susceptibility was determined by the Kirby-Bauer disk diffusion technique. A conventional PCR was used for the detection of Salmonella invA, agfA, lpfA and sivH virulence genes. Of the 446 offal samples, 13 tested positive for Salmonella (2.91%; CI = 1.6-5). The serovars included S. Enteritidis (n = 3/13), S. Mbandaka (n = 1/13), S. Infantis (n = 3/13), S. Heidelberg (n = 5/13) and S. Typhimurium (n = 1/13). Antimicrobial resistance against amoxicillin, kanamycin, chloramphenicol and oxytetracycline was found only in S. Typhimurium and S. Mbandaka. All 13 Salmonella isolates harboured invA, agfA, lpfA and sivH virulence genes. The results show low Salmonella prevalence from chicken offal. However, most serovars are known zoonotic pathogens, and multi-drug resistance was observed in some isolates. Consequently, chicken offal products need to be treated with caution to avoid zoonotic Salmonella infections.

Assessing growth performance and survival of wild shad Tenualosa ilisha fry in two earthen ponds with different feeding regime: A new insight for conservation

The present study describes the rearing and growth performance of Hilsa (Tenualosa ilisha) fry in experimental trial performed in earthen pond ecosystem located adjacent to river Rupnarayan for developing in-situ rearing protocol of fry collected from the wild sources. The seed was collected during May, 2020 from the river and stocked in two different rearing ponds. One of the ponds was provided with artificial feed and another fed with chicken offal to raise the production of zooplankton. Routine monitoring of water and soil quality parameters as well as other biotic communities like plankton was performed to understand the dynamics of ecological conditions in relation to changes in length and weight. The results of PCA between sediment quality and specific growth of fish indicated the strong positive loading of available nitrogen, available phosphate and free calcium carbonate, while moderate positive loading was observed with percentage of silt. Interestingly, it is observed that survivability rate of fish was approximately 80% in both the ponds. The results indicated natural growth fish was considerably well in both the experimental ponds. However, higher growth of juveniles were observed in the pond 2 with higher phytoplankton density (9120cells/L) than pond 1 having lower phytoplankton density (920cells/L) at the end of the experiments. The water quality parameters were comparable in both the experimental ponds. Based on this study it is evident that plankton (especially zooplankton) has induced better growth in pond 2 and possible incorporation of live feed along with formulated feed might trigger better growth of the stocked Hilsa. The present study provides new insight in developing field based approaches for successful rearing and conservation of threatened Hilsa in Indian waters.

Antibiotic-Resistant Bacteria and Antibiotic Residue Contamination in Fresh Raw Foods Sold at Wholesale Markets in Thailand

Objective: To investigate the extent of antibiotic-resistant bacteria and antibiotic residue contamination in fresh raw foods sold at wholesale markets in Thailand, which may be the important drivers of antibiotic-resistant bacteria colonization and antibiotic-resistant bacterial infection in Thai population. Materials and Methods: Fresh raw foods, including food from animal products, seafoods, vegetables, fruits, and honey were purchased from two large wholesale markets in Thailand. Food samples were cultured for antibiotic-resistant bacteria and tested for the presence and amount of antibiotic residue. Results: Among 521 samples for bacterial culture, 86.9% grew at least one kind of bacteria. Enterobacteriaceae were commonly isolated and were commonly resistant to ampicillin (76.7% to 100%). ESBL-producers and ceftriaxone-resistant Enterobacteriaceae were prevalent in swine and duck samples (56.7% to 91.7%). Some isolates were resistant to co-amoxiclav (13.3% to 60.0%) and cefoxitin (5.0% to 30.0%). Colistin-resistant Enterobacteriaceae were observed in pork meat (1.4%) and chicken offal (7.0%). Ertapenem-resistant Enterobacteriaceae were detected in cha-om (26.7%). Among 501 samples for antibiotic residue testing, 37.1% contained at least one antibiotic residue. Enrofloxacin was the most prevalent antibiotic residue, followed by doxycycline and tilmicosin. Although most samples contained less antibiotics than the maximum residue limit (MRL), 7.0% contained an amount of at least one antibiotic above the MRL. Conclusion: Many fresh raw foods sold at wholesale markets in Thailand were contaminated with antibiotic-resistant bacteria, and some contained antibiotic residues. Therefore, Thai people are at risk of being colonized with antibiotic-resistant bacteria and developing antibiotic-resistant bacterial infection due to consuming foods contaminated with antibiotic-resistant bacteria or containing antibiotic residues. Keywords: antibiotic-resistant bacteria, antibiotic residue, fresh raw foods, wholesale market, Thailand

PROTEIN AND ENERGY UTILIZATION BY WEANERS PIGS FED CHICKEN OFFAL MEAL OR BLOOD MEAL WITH GRADED LEVELS OF MAIZE OFFAL

Thirty six weaner pigs (Large White x Landrace) averaging 8.86 ± 0.22kg body weight were randomly assigned to six isonitrogenous (20% crude protein) dietary treatment groups in a 2 x 3 factorial design of two protein sources (Blood Meal (BM) and Chicken Offal Meal (COM) and three replacement levels of maize offal (MO) for maize (0, 25 and 50%) in a fifty six (56) day feeding trial after an adjustment period of seven days. Dry matter intake was not significantly (P &gt; 0.05) affected by feeding COM or BM at the three levels of MO. The pigs fed the 0 and 25% levels of MO for both protein sources had comparable (P &gt; 0.05) growth performance. They performed significantly (P &lt; 0.01) better than for those fed BM. The metabolisable energy intake per day was not significantly (P &gt; 0.05) affected by varying MO levels and the protein sources. Conversely, the metabolisable energy intake per kilogramme gain was significantly (P &lt; 0.05) influenced by both treatments. The cost of feed per kg live weight gain followed the same pattern as the performance parameters. The 0 and 25% MO diets with comparable costs were more cost effective (P &lt; 0.05) than the 50% MO diets while COM supported cheaper cost of feed per gain. The serum metabolites were not significantly (P &gt; 0.05) influenced by the varying MO levels and the protein sources except for the urea and globulin contents. The results establish the fact that weaner pigs can tolerate maize offal inclusion in feed up to 25% but not up to 50% (P &lt; 0.01) and that COM enhanced performance than does BM as an unconventional protein supplement in the diet for weaner pigs.

Substitution effect of fish meal with various sources of animal by‐product meals in feed on growth, feed utilization, body composition, haematology and non‐specific immune response of olive flounder ( Paralichthys olivaceus , Temminck &amp; Schlegel, 1846)

Suitability of various animal by-product meals was evaluated as an alternative source for fish meal (FM) in olive flounder feed when 30% FM was substituted. Juvenile fish (n = 480) was distributed into 24 flow-through tanks. Seven experimental diets were prepared. 65% FM and 12% soybean meal were included as the protein source in the control (Con) diet. Thirty per cent of FM was substituted with tuna by-product, chicken by-product, hydrolysed chicken offal, meat, meat and bone, and blood meals, referred to as the TBM, CBM, HCOM, MM, MBM and BM diets respectively. Each diet was hand-fed to satiation for 8 weeks. Weight gain, specific growth rate (SGR) and feed efficiency ratio (FER) of fish fed the TBM diet were greater than those of fish fed all other diets. Weight gain, SGR and FER of fish fed the Con diet was also greater than those of fish fed the MM and BM diets. The proximate composition of the whole body fish was unaffected by the experimental diets. Lysozyme activity of fish fed the Con, TBM and CBM diets was higher than that of fish fed the MM and BM diets. Amino acid profiles of fish were not affected by the experimental diets except for tryptophan. All fatty acid profiles of fish except for eicosadienoic acid were affected by the experimental diets. In conclusion, tuna by-product and chicken by-product meals are the good substitutes for FM in olive flounder feed when 30% of FM at 65% in diet was replaced with animal by-product meals.

GROWTH AND SOME CARCASS CHARACTERISTICS OF GROWING PIGS FED FULL-FAT SOYBEAN-BASED CONTAINING THREE PROTEIN SUPPLEMENTS

Thirty-six large White X landrace growing pigs were randomly in a 3 x 3 factorial arrangement. The two dietary variables were protein source (Fish meal FM Chicken offal meal-COM and full-fat soybean - FFSB) and processing time of FFSB (30, 60, 90 minutes). Pigs on 90 minutes processed FFSB performed slightly better than those on 60 and 30 minutes as regard growth rate and feed to gain ration. The diets had no significant effect on slaughtering performance. However, the general trend showed a numerical increase in estimated loin eye area, back fat and abdominal fat with increased processing time of FFSR. Chicken offal meal also slightly increased the backfat and abdominal fat

Comparison of Antimicrobials and Hormone-Like Anabolic Agents Residuals in Chicken Meat and Their Offal Under Two Different Country Legislation

Antibiotics and hormone-like anabolic agents use in order to intensify the production of chicken farms. These may reflect on formation the harmful chemical residues in their meat and offal. The purpose of the current study was to analyze some chemical hazards in local and imported chicken meat and their edible offal including: Quantitative assessment of ciprofloxacin and sulfonamide and growth promoters’ 17β-Estradiol. A total of 214 fresh and imported chicken carcasses were purchased from different commercial chicken markets at Ismailia governorate, Egypt and Bahrain. 10 grams of meat sample was blended, centrifuged, homogenized, extracted and analyzed by HPLC to estimate the concentration levels of ciprofloxacin and 17β-Estradiol residues. Spectrophotometer used to detection of sulfonamide residues. The mean concentrations ciprofloxacin values for Egyptian and Bahrainis chicken meat were; 0.339 ± 0.026 and 0.126 ± 0.031 µg/g respectively. While, the mean concentrations ciprofloxacin values for Egyptian and Bahrainis chicken offal were; 0.65 ± 0.075 and 0.545 ± 0.045 µg/g respectively. The mean concentrations Sulfadiazine values were; 0.020 ± 0.003 and 0.006 ± 0.002 µg/g respectively while, in chicken offal were 0.045 ± 0.020 and 0.025 ± 0.015 µg/g respectively. The mean concentrations 17β-Estradiol values for chicken meat were; 1.626 ± 0.076 and 1.593 ± 0.069 µg/g respectively. While, in chicken offal were 2.60 ± 0.089 and 2.95 ± 0.075 µg/g respectively. Results of this study observed that the Egyptian chicken meat under investigation contained several chemical residues than Bahrain samples which may represent a sever public health hazards for consumers if increase their daily intake level of chicken meat. In addition to, such chemical residues were more concentrated in the chicken offal than chicken meat. More attention from the concerned authority must be considered in controlling antibiotics and hormone-like anabolic agents at chicken farms.

Valorization of chicken slaughterhouse by-products: Production and properties of chicken trachea hydrolysates using commercial proteases

ABSTRACT Collagen is an abundant structural protein found in many organs of animals, for example skin, bones, and connective tissues. Chicken trachea is a collagen-rich fraction of chicken offal that is converted to a solid waste stream during poultry processing. Collagen hydrolysates and peptides have unique biological properties, which is potentially converted from chicken trachea and can be used as a useful functional ingredient. Fresh chicken trachea was sterilized by autoclaving at 121°C for 30 min and dried using freeze drier to obtain chicken trachea flakes (CTF). CTF had 68.56% protein with the 50% solubility at pH 6–10. The enzymatic hydrolysis of chicken trachea was performed using Alcalase®, Flavourzyme®, Protamex®, and Papain under the optimal condition of each enzyme. An improvement in solubility was observed. Chicken trachea hydrolysates (CTH) with the highest solubility were detected in samples hydrolyzed with Alcalase®, follow by Protamex®, Papain, and Flavourzyme®, respectively. Within an hour of hydrolysis, the CTH obtained from Alcalase® hydrolysis had the highest total amino acid contents. The top three amino acids found in this hydrolyzate powder were Glutamic acid (Glu), Glycine (Gly), and Aspartic acid (Asp). Moreover, this sample exhibited the greatest antioxidant and bioactive properties as shown by the highest antioxidant capacity from DPPH and FRAP assay (4.42 TEAC µMol/mg CTH and 22.48 TEAC µMol/mg CTH, respectively) and the lowest IC50 of ACE I inhibitor (0.41 mg/mL). These results suggested that Alcalase® hydrolysis provided the chicken trachea collagen hydrolyzate with unique properties that could be used in various food systems.

Salmonella Serovars Isolated from Retail Meats in Tokyo, Japan and Their Antimicrobial Susceptibility

To identify the risk of Salmonella in meat, we investigated the prevalence of Salmonella, serovars and their antimicrobial susceptibility patterns. Salmonella was found in 353 out of 849 (41.6%) chicken, 9 out of 657 (1.4%) pork, 1 out of 517 (0.2%) Beef, 6 out of 8 (75.0%) chicken offal, 43 out of 142 (30.3%) pork offal and 4 out of 198 (2.0) beef offal samples collected from retail meats in Tokyo, Japan between 2009 and 2017. Salmonella Infantis was the most common serovar, followed by S. Schwarzengrund in the isolates from domestic chicken meats. The prevalence rate of S. Infantis decreased while that of S. Schwarzengrund increased by the year. Apart from this, the most prevalent serovars were S. Heidelberg in the imported chicken meat isolates, S. Typhimurium and Salmonella O4:i:- in pork, and S. Derby in beef isolates. Antimicrobial testing revealed high resistance to tetracycline (TC) in all meat sample isolates; however, all the isolates were sensitive to carbapenem and fluoroquinolone. Fourteen cefotaxime (CTX) resistant strains, seven extended spectrum β-lactamase (ESBL) producing strains and twenty-three AmpC producing strains were isolated from chicken meat samples. These findings indicate that the serovar and antimicrobial susceptibility varied among meat samples.

Carry-over of some Fusarium mycotoxins in tissues and eggs of chickens fed experimentally mycotoxin-contaminated diets

Fusarium mycotoxins are fungal contaminants found in different crops intended for human and animal consumption. Due to the co-occurrence of several of mycotoxins, the present study aimed at examining the transfer of these toxins into tissues of broiler chickens and eggs of laying hens fed contaminated diets. After an adaptation period, the chickens were fed contaminated diets containing mg/kg levels of deoxynivalenol (DON), enniatins (ENN A, A1, B, B1) and beauvericin (BEA) and high μg/kg levels of HT-2 toxin (HT-2), T-2 toxin (T-2) and zearalenone (ZEN) during a repletion period of two weeks, followed by a depletion period of two weeks. DON, ZEN, T-2 and HT-2 were not carried out into the skin and the liver of broiler chickens. ENN B (20.5 ± 6.6 μg/kg) and BEA (162 ± 55 μg/kg) were found in the liver, while in the skin their respective concentrations were 50 ± 17 μg/kg and 120 ± 16 μg/kg during the first week of the repletion period. Carry-over rates into liver and skin were higher for BEA (1.6% and 1.2%, respectively) than for ENNs (0.1 and 0.4%, respectively). During the depletion period, ENNs and BEA were eliminated from the skin and the liver. ENN B, ENN B1 and BEA were carried over into eggs at 0.1%, 0.05% and 0.44% upon 2–3 days of feeding the contaminated diet, respectively. These transfers were fully eliminated 9–10 days after feeding the control diet again. These results indicate the transfer of ENN B, ENN B1 and BEA from feed to chicken offal, meat products and eggs at a very low degree, thus marginally contribute to the total dietary intake of these fusariotoxins for consumers. Nevertheless, taking precautionary measures in the field, harvest, transport and storage of the raw materials is required to keep the mycotoxin concentration in feed below the safe levels.

Risk of acquiring listeriosis from consumption of chicken offal among high risk group

Listeria monocytogenes causes mild foodborne infection in healthy adults but serious complication in high risk group such as infant, pregnant women, immunocompromised and elderly. This study aims to estimate the probability of infection based on the survivability of L. monocytogenes in chicken offal, particularly chicken liver, gizzard and heart. A total of 20 µl of L. monocytogenes with inoculum size of 9.02 log CFU/ml was spiked on the chicken offal and stored at room temperature (28oC) for 0, 1, 3, and 6 h. This experimental design was aimed to simulate the contaminated chicken offal stored at temperature danger zone. It was found that chicken offal supported the growth of L. monocytogenes that it significantly grows at 3 and 6 h incubation. Risk assessment was performed by combining this result with the data from our previous studies as well as data from government agencies and other studies. Dose-response model was used to estimate the probability of listeria infection per year. Immunocompromised was the highest risk group, followed by pregnant women, elderly and general population with probability of infection per year estimated at 3.78 x 10-3, 1.76 x 10-4, 5.68 x 10-8 and 5.75 x 10-9, respectively. It can be concluded that high risk group requires extra caution in their food consumption in order to prevent listeriosis which can result in serious complication and death.

New poultry meat product

In the modern world, an increasing number of people prefer poultry sausages, since this product has a low calorie level. In addition, poultry sausages contain less fat and cholesterol, which is ideal for people who have a healthy and balanced diet. The creation of new recipes for products from poultry meat with the inclusion of components of animal and vegetable origin is aimed at increasing meat resources and improving the quality of finished products. In many cases, the addition of new components, as well as changing the ratio and methods of introduction into the recipe, can increase the nutritional value of the finished product. A chicken roll recipe was developed using chicken offal, Unicell-500 hydrated wheat fiber and heat-treated carrots. Kiwi was used to soften offal. Organoleptic, physico-chemical and microbiological studies of chicken rolls, developed according to classical technology and a new recipe, were carried out. The research results showed that the use of carrots and offal in chicken roll improves the taste of the product, allows you to reduce calorie content and get the product with the necessary consistency, improve functional properties, improves economic performance by reducing the cost of finished products from poultry meat. The use of Unicell-500 dietary fiber reduces the consumption of expensive meat raw materials due to the high moisture-binding ability of fiber. Enrichment of chicken roll allowed to improve physico-chemical, organoleptic, microbiological indicators.

Efficacy of household washing pre-treatments and cooking methods for reduction of Listeria monocytogenes in artificially contaminated chicken offal

Consumption of chicken offal is common and famous among Malaysians as it is often served as one of the side dishes with rice. Chicken offal can be a potential source of Listeria monocytogenes because slaughtered animals are recognized as a reservoir for foodborne pathogens. L. monocytogenes is a dangerous foodborne pathogen which can cause severe foodborne listeriosis with high fatality rate. This study aimed to determine the efficacy of different washing pre-treatment and cooking methods to reduce L. monocytogenes in artificially contaminated chicken offal. All the washing pre-treatments (dip treatment in different water sources and wash treatment with different water flow rates) showed significant reduction of the pathogen (p&lt;0.05) when the inoculated samples were treated from 2 mins onwards. Washing the inoculated samples under the water flow rate of 2 L/min was the most effective way to reduce the number of L. monocytogenes (approximately 1.97 log reduction after washing for 10 mins). For heat treatment study, deep-frying was the most effective cooking method followed by boiling and pan-frying to reduce L. monocytogenes where all L. monocytogenes cells (7.91 log10 CFU/g) were killed within 45 s under deep-frying treatment. Overall, the study indicated that washing under running tap water (2 L/min) and deep-frying was effective in reducing and controlling the microbial populations during food preparation. The findings from this study can serve as a safe preparation step and cooking guideline. It is necessary to implement safe steps in food handling practices among food handlers to minimize the risk of foodborne infection.

Nutritional composition, sensory evaluation and Halal perspective African Catfish (Clarias gariepinus) fed with diets containing najasa (impurities)

Lately, Muslim consumers in Malaysia, are avoiding catfish (Clarias gariepinus) owing to the uncertainty of its halal status. This is due to the use of unclean/impure substances (najasa in Islamic law) as feed from animal carcasses or feed incorporated with animal waste (fat, blood or bone). Under the islamic law, animals which consume large quantities of najasa and led to noticeable changes of its odour, colour and taste of the meat are haram (forbidden) or makruh (undesirable). To our knowledge there is no investigation on whether the feed could alter the odour, taste or colour of the catfish from this perspective. Thus a study was undertaken to evaluate if feeding diets comprising of najasa will affect the nutritional composition and sensory characteristics of catfish.The fish were fed with imported pellets (consisting of 10-15% blood meal with porcine DNA detected), local pellets and 100% chicken offal for 12 weeks. The results showed treatment with imported pellets do not have a significant (p&gt;0.05) effect on the nutritional composition (proximate composition, amino acids and fatty acids profiles), colour, odour and flavour of catfish fillet, but significantly (p&lt;0.05) change the odour and flavour.

Prevalence of Microbial Contamination and Antibiotic Residue in Chicken Meat, Beef, and Offal in South Sulawesi

Chicken meat, beef, and offal are important animal products. They easily spoiled due to bacterial contamination and might contain antibacterial residues derived from treatments during rearing and processing. This present work aimed to investigate location- and time-dependent value of TPC (Total Plate Count), and analyze antibiotic residue present in chicken meat, beef, and offal sampled in the Province of South Sulawesi. This current study was conducted by analyzing data released from UPT. Animal Product Quality Analysis in Department of Animal Husbandry and Animal Health, Province of South Sulawesi. Our experimental design was arranged according to completely randomized design with factorial (4×3), and carried out 5 replications. The results showed that two locations (Takalar District and Palopo District) had the lowest TPC value, while Palopo City possessed the highest TPC value. Regarding to antibiotic residue, the positive sample was only found in 2015, a chicken meat sample collected in Luwu Utara District.Keywords : Total Plate Count (TPC), Antibiotic Residues.