Chicken Manure Compost Research Articles

An assessment of the persistence of pathogenic bacteria removal in chicken manure compost employing clay as additive via meta-genomic analysis.

The aim was to evaluate pathogenic bacteria (PB) survival during the composting of chicken manure (CM) amended with five different dosages of clay compared to CM without clay-applied treatment. The results showed that 85-87% of PB relative abundances (RAs) were significantly reduced in lower dosages of applied clay (T2 and T3). However, the maximum survival of PB was noticed in the T6 and T5 treatments, but most of the PB belong to Firmicutes, Actinobacteria and Proteobacteria phylum and their derivative bacterial species. The changes in PB during the composting were not only strongly influenced by clay amendment but also significantly associated with the succession of bacterial species in compost. Bacillus, Clostridium, Mycobacterium and Klebsiella were the dominant spore-forming bacteria identified in higher dosages of clay (i.e., T4, T5 and T6) treatments, but very low abundance of these bacterial genus and its species were recovered from lower dosages of clay (T2 and T3)-applied treatments. Overall, without clay, amended-CM-derived compost contained a relatively higher PB abundance than other treatments, as the anaerobic bacterial species Clostridium_difficile_AA1, Vibrio_cholerae, and Acinetobacter_calcoaceticus had relatively greater RAs, followed by Klebsiella_oxytoca_10-5248, Paenibacillus_Bacillus_cereus and Bartonella_quintana_RM-11. Thus, CM composting with 4% clay amendment is considered a useful method for the efficient recycling of CM, as this process produced sanitized compost with less survival of PB.

Evaluating the impact of bamboo biochar on the fungal community succession during chicken manure composting

The objective of this study was to investigate the fungal community succession and variations in chicken manure (CM) compost with different concentration of bamboo biochar (BB) as additive via the using of metagenomics method. The consequent obviously revealed that Chytridiomycota, Mucoromycota, Ascomycota and Basidiomycota were the dominant phylum, while Batrachochytrium, Funneliformis, Mucor, Phizophagus and Pyronema were the pre-dominant genera in each treatment. Redundancy analyses indicated that higher dosage of biochar applied treatments has significant correlation between fungal communities and environmental factors. The diversity of fungal community was analogous but the relative abundance (RA) was inconsistent among the all treatments. In addition, the principal component analysis was also confirmed that T5 and T6 treatments have considerably correlation than other treatments. However, the mean value of RA remained maximum in higher dosage of biochar blended treatments. Ultimately, the RA of different fungal genus and species were influenced in CM compost by the BB amendment.

Effects of chicken manure compost and high percentage of biochar on container-grown basil (Ocimum basilicum)©

Effects of chicken manure compost and high percentage of biochar on container-grown basil (<i>Ocimum basilicum</i>)^©

Effects of amendments on phosphorous status in soils with different phosphorous levels

Phosphate fertilizers are used in horticultural production to increase yields, but overuse can result in inefficient crop uptake and large loss of phosphorus (P). Soil amendments could potentially reduce the risk of P loss while maintaining high crop yield. The objective of this study was to investigate the effect of organic and inorganic amendments on P fractions in soils with different P levels (low, medium and high), and the underlying mechanisms. Five treatments were included: no amendment (CK), chemical fertilizers (CF), rice straw (FS), chicken manure compost (CM), and vermicompost (VM). Counterintuitively, the proportion of organic phosphorus (OP) tended to increase in the CF treatment, especially in soil with low P level. Results indicated that the change in the proportion of OP was mainly controlled by soil acid phosphomonoesterase, alkaline phosphomonoesterase and phosphate-solubilizing microorganisms. The proportion of available P increased in soil with low P level, but decreased in soil with high P level in the VM treatment. Non-labile P was preferentially formed in the VM treatment because of the high calcium content in vermicompost. Phosphates associated with aluminum (Al-P) were significantly higher in the CM treatment than in other treatments. This treatment also had a large presence of Pseudomonas and Streptomyces bacteria, which are capable of promoting the formation of Al-P. Our results indicated that mechanisms causing changes in soil P fractions were distinctly different among various amendments, and that vermicompost had the greatest potential to increase P supply in soil with low P level, and to decrease the risk of P loss in soil with high P level.

How does manganese dioxide affect humus formation during bio-composting of chicken manure and corn straw?

The aim of this study is to reveal the roles of MnO2 in Maillard reaction of biotic composting, and to identify its effectiveness in promoting humus formation. Corn straw (CS) and chicken manure (CM) have been chosen to be composted. During CS composting, addition of MnO2 rapidly reduced reducing sugars concentration (decreased by 84.0%) in 5 days and significantly improved humus production by 38.7% compared with treatment without MnO2. Whereas in CM composting, the promoting effect of MnO2 on humus formation was relatively weak by comparing with the treatment group of CS. Additionally, the presence of MnO2 has reshaped bacteria community, which might be the reason of MnO2 stimulated bacteria to utilize organic matter during CM composting. Therefore, the structural equation modeling has confirmed that MnO2 mainly performed as chemical catalyst to promote humus formation during CS composting. Besides catalyst, MnO2 also played as a bioactive activator in CM composting.

Composting of chicken manure with a mixture of sawdust and wood shavings under forced aeration in a closed reactor system

PurposeThis study aimed to achieve successful composting and aeration rate and to optimize the carbon:nitrogen (C:N) ratio to provide favourable conditions for the process. In the current experiment, investigation were made on variations in physico–chemical properties, i.e., temperature, ammonia and carbon dioxide emissions, pH, electrical conductivity (EC), organic matter (OM) and seed germination index (GI%) of composting chicken manure mixed with sawdust and wood shavings under different aeration rates in a closed reactor system.MethodsThree cylindrical reactors (total volume, 60 L) were used with three aeration rates of 0.25, 0.50 and 0.75 L min−1 kg−1 OM. The air was dispensed from the bottom of an air compressor. The ambient and compost temperature were monitored thrice daily over 30 days of composting.ResultsThe highest temperatures were 56.9, 55.8 and 48.1 °C, with 0.25, 0.50 and 0.75 L min−1 kg−1 OM aeration rates, respectively. At the end of composting, lowest ammonia and carbon dioxide emissions were observed with 0.25 L min−1 kg−1 OM aeration, indicating that this compost was more stable than other composts. The lowest GI was recorded on day 30 with 0.75 L min−1 kg−1 OM aeration, indicating severe phytotoxicity in the substrate. Maximum OM degradation occurred with 0.25 L min−1 kg−1 OM aeration.ConclusionThis study, therefore, suggested that 0.25 L min−1 kg−1 OM aeration in the composing of the chicken manure mixed with sawdust and wood shavings in closed a reactor system provided the most favourable conditions for maturation.

New insight of tertiary-amine modified bentonite amendment on the nitrogen transformation and volatile fatty acids during the chicken manure composting

In this study, the main objective was to investigate the potential effect of tertiary-amine modified bentonite (TAMB) on the nitrogen transformation and the volatile fatty acids (VFAs) degradation during the chicken manure (CM) composting. Six dosages of TAMB (0%, 2%, 4%, 6%, 8% and 10%) were amended into the mixture of CM and wheat straw and then composted for 50 days. The results revealed the TAMB amendment could prolong the thermophilic phase and enhance the organic matter (OM) degradation. With the increasing dosage of TAMB, the ammonia volatilization was reduced by 15.41%–65.35%. Meanwhile, the TAMB addition had a positive effect on VFAs degradation and reducing odor unitMAX (OUMAX) by 17.61%–59.24%. Moreover, CH4 was reduced by 12.15%–32.78% in TAMB applied treatments compared to control. Finally, combined with all results, it indicated that TAMB amendment could reduce VFAs, CH4 emission and nitrogen loss to improve the compost quality.

Enhanced Growth and Activities of the Dominant Functional Microbiota of Chicken Manure Composts in the Presence of Maize Straw

As a consequence of intensive feeding, the bulk deposition of livestock manure causes severe environmental problems. Composting is a promising method for waste disposal, and the fermentation process is driven by microbial communities. However, chicken manure contains diverse gut microbes, mainly species derived from Proteobacteria, which may include pathogens that threaten human health. To evaluate composting as a harmless treatment of livestock manure, the dynamics of the microbiota in two chicken manure composts were studied, and the influences of adding maize straw on the compost microbiota were compared. The results revealed that microbes from Firmicutes including Bacillus and Lentibacillus are the most dominant degraders with a strong amino acid metabolism, and they secrete a diverse array of proteases as revealed in metaproteomics data. The addition of maize straw to the chicken manure compost accelerated species succession at the initial stage, and stimulated carbohydrate metabolism in the dominant microbiota. Besides, under the resulting high temperature (>70°C) conditions, the relative abundance of Proteobacteria was reduced by 78% in composts containing maize straw by day 4, which was faster than in compost without added maize straw, in which the abundance was reduced by 66%. Adding maize straw to chicken manure composts can therefore increase the fermentation temperature and inhibit the growth of Proteobacteria. In general, these findings provide increased insight into the dynamic changes among the dominant functional microbiota in chicken manure composts, and may contribute to the optimization of livestock manure composting on an industrial scale.

N2O and CO2 emissions following repeated application of organic and mineral N fertiliser from a vegetable crop rotation

Accounting for nitrogen (N) release from organic amendments (OA) can reduce the use of synthetic N-fertiliser, sustain crop production, and potentially reduce soil borne greenhouse gases (GHG) emissions. However, it is difficult to assess the GHG mitigation potential for OA as a substitute of N-fertiliser over the long term due to only part of the organic N added to soil is being released in the first year after application. High-resolution nitrous oxide (N2O) and carbon dioxide (CO2) emissions monitored from a horticultural crop rotation over 2.5 years from conventional urea application rates were compared to treatments receiving an annual application of raw and composted chicken manure combined with conventional and reduced N-fertiliser rates. The repeated application of composted manure did not increase annual N2O emissions while the application of raw manure resulted in N2O emissions up to 35.2 times higher than the zero N fertiliser treatment and up to 4.7 times higher than conventional N-fertiliser rate due to an increase in C and N availability following the repeated application of raw OA. The main factor driving N2O emissions was the incorporation of organic material accompanied by high soil moisture while the application of synthetic N-fertiliser induced only short-term N2O emission pulse. The average annual N2O emission factor calculated accounting for the total N applied including OA was equal to 0.27 ± 0.17%, 3.7 times lower than the IPCC default value. Accounting for the estimated N release from OA only enabled a more realistic N2O emission factor to be defined for organically amended field that was equal to 0.48 ± 0.3%. This study demonstrated that accounting for the N released from repeated application of composted rather than raw manure can be a viable pathway to reduce N2O emissions and maintain soil fertility.

Manipulation of the rhizosphere microbial community through application of a new bio-organic fertilizer improves watermelon quality and health.

Bio-organic fertilizers (BOFs) combine functional microbes with a suitable substrate and have been shown to effectively suppress soil-borne diseases and promote plant growth. Here, we developed a novel bio-organic fertilizer (BOF) by fermentation of a cow plus chicken manure (M) compost using Fen-liquor Daqu (FLD) as a fermentation starter and compared the compositions of bacterial and fungal communities in the rhizosphere soil of watermelon plants after treatment with different fertilizers. Further, we aimed to explore the mechanisms underlying plant-promoting and disease (Fusarium wilt)-suppressing activities of each rhizosphere microbial community. The microbial communities of soil amended with cow plus chicken manure compost (S+M), soil amended with the BOF (S+BOF), and untreated control soil (S) without plants were analyzed through sequence analysis using the Illumina MiSeq platform. The results showed that a new microbial community was formed in the manure compost after fermentation by the Daqu. Application of the BOF to the soil induced remarkable changes in the rhizosphere microbial communities, with increased bacterial diversity and decreased fungal diversity. Most importantly, S+BOF showed the lowest abundance of Fusarium. Moreover, watermelon quality was higher (P < 0.05) in the S+BOF than in the S+M treatment. Thus, application of the BOF favorably altered the composition of the rhizosphere microbial community, suppressing Fusarium wilt disease and promoting plant quality.

Is Composting a Route to Solubilize Low-Grade Phosphate Rocks and Improve MAP-Based Composts?

ABSTRACT: In alkalinized and Ca-rich composts, solubilization of apatite from phosphate rocks (PRs) is not guaranteed; however, chelating agents and humified substances produced during composting may alter the soluble contents and P forms of monoammonium phosphate (MAP)-based composts. These effects may depend on the proportions of organic wastes and P source used in the compost piles. The aim of this study was to evaluate the effect of composting chicken manure, coffee husk, and Araxa PR, Bayovar PR, or MAP in [...]

Antibiotic Uptake Reducing Effect of Zeolite and Shell Meal Fertilizer Amendment for Lettuce (Lactuca sativa L.) Cultivation Fertilized with Chicken Manure Compost

Antibiotic Uptake Reducing Effect of Zeolite and Shell Meal Fertilizer Amendment for Lettuce (Lactuca sativa L.) Cultivation Fertilized with Chicken Manure Compost

The rhizosphere microbial community response to a bio-organic fertilizer: finding the mechanisms behind the suppression of watermelon Fusarium wilt disease

We aimed to evaluate the capability of bio-organic fertilizer suppressing watermelon Fusarium wilt disease, compare the variations of the rhizosphere bacterial and fungal community compositions after treatment with different fertilizers, and explore mechanisms causing disease suppression in rhizosphere microbial community. A rhizobacterium (Bacillus amyloliquefaciens JDF35) was identified to control watermelon Fusarium wilt disease. Bio-organic fertilizer JDF35 (BOF) was generated by inoculating JDF35 into the organic fertilizer (OF) composed of cow and chicken manure compost (1:50 v/w). A three successive growing season pot experiment was designed to evaluate the effects of BOF compared with OF and chemical fertilizer (CF). Next-generation sequencing using the Illumina MiSeq platform was used to investigate the variations in rhizosphere microbial community composition. The growth of the watermelon plants, soil pH, and available N, P and K concentrations were the highest in the BOF treatment. The Fusarium wilt incidence in the BOF treatment was lower than that in the CF and OF treatment, and the differences for disease incidence were significant (P < 0.001). The diversity of the rhizosphere bacterial community was higher, and that of the fungal was lower in the BOF treatment. Most importantly, the BOF treatment had lowest abundances of Fusarium. The application of the BOF altered the composition of rhizosphere microbial community, suppressing Fusarium wilt disease and promoting plant growth.

PEMANFAATAN LIMBAH KOTORAN AYAM SEBAGAI BAHAN BAKU PEMBUATAN KOMPOS

Farm poultry, especially chicken, is a producer of meat and eggs. In addition to producing meat and eggs, chicken farms also have negative impacts, which can cause odorless gas that can pollute the environment. In the village there are six places Srikaton laying chicken farms that always make chicken manure every day. Alternative technology that can be applied and has a positive outlook is to convert poultry farm waste, ie chicken manure into compost. IBM outer target groups laying chicken farming is to produce compost chicken that can be utilized by the villagers Srikaton (short-term targets), and the long-term target is to sell the compost to other consumer good in the village Srikaton, and outside the village Srikaton . Effective technology for the transformation of society is using extension methods, training by making demonstrations, or direct practice coupled with mentoring. The target of this service is Srikaton village community, which consists of chicken breeders, youth organization group, the PKK, and residents who do not have a job (unemployed). Based on the activities carried out, the people were very enthusiastic. Through these activities, the village Srikaton has the skills to produce compost chicken manure that can be used for fertilizer in the garden itself, and or for sale, so as to improve their welfare. Keywords: chicken manure, compost

Evolution of various fractions during the windrow composting of chicken manure with rice chaff

Different fractions during the 85-day windrow composting were characterized based on various parameters, such as physiochemical properties and hydrolytic enzyme activities; several technologies were used, including spectral scanning techniques, confocal laser scanning microscopy (CLSM) and 13C Nuclear Magnetic Resonance Spectroscopy (13C NMR). The evaluated parameters fluctuated strongly during the first 3 weeks which was the most active period of the composting process. The principal components analysis (PCA) results showed that four classes of the samples were clearly distinguishable, in which the physiochemical parameters were similar, and that the dynamics of the composting process was significantly influenced by C/N and moisture content. The 13C NMR results indicated that O-alkyl-C was the predominant group both in the solid and water-soluble fractions (WSF), and the decomposition of O-alkyl-C mainly occurred during the active stage. In general, the various parameters indicated that windrow composting is a feasible treatment that can be used for the resource reuse of agricultural wastes.

Study on Effects of Different Medium Compositions on Growth and Seedling Quality of Two Tomato Varieties Under Greenhouse Conditions

ABSTRACTThis research was conducted to investigate the effects of different medium compositions on physicochemical properties and the growth of two tomato varieties. Five treatments were applied, combining medium composition with rice husk ash (RHS) and coconut fiber (CF) with the following proportions: 1/3 Peat moss + 1/3 rice husk ash + 1/3 coconut fiber (T1); 1/3 Vermicompost + 1/3 rice husk ash + 1/3 coconut fiber (T2); 1/3 Cattle manure compost + 1/3 rice husk ash + 1/3 coconut fiber (T3); 1/3 Chicken manure compost + 1/3 rice husk ash + 1/3 coconut fiber (T4); 1/3 Hog manure compost + 1/3 rice husk ash + 1/3 coconut fiber (T5). The results demonstrated that between pH, electrical conductivity (EC) value, and nutrient content in the media, there was a significant difference that led to different concentrations of total macro- and micronutrients in the shoots of both varieties. In addition, the root weight, shoot weight, and root volume were affected by medium composition for two varieties in seedling stage. A mixture of cattle manures composted with RHS and CF under the rate (1:1:1 by volume), respectively, gave the highest value of germination rate, plant height, leaf number, and plant biomass. Medium composition significantly influenced the germination rate and elongation of seedlings, specifically for each tomato variety. Therefore, cattle manure compost as a medium supplement improves seedling quality and growth of the two tomato varieties.

The Effects of Fresh and Composted Chicken Manures on Some Soil Characteristics

ABSTRACTThe effects of fresh and composted chicken manures on some soil characteristics were investigated. Fresh and composted chicken manures were mixed with the soil at the rates of 0%, 1%, 2%, 3%, and 4%, and then were incubated for 90 days. At the end of this incubation period, some soil physical properties closely related to soil aggregation (such as hydraulic conductivity, water stable aggregates, aeration porosity, and available water) and some soil chemical characteristics (such as organic carbon, total nitrogen, pH, and electrical conductivity, EC) were determined. As doses of both manures in the soil mixtures increase, the amounts of water stable aggregates, hydraulic conductivity, and aeration porosity increased, whereas the available water content decreased. Increasing doses of both manures increased the amount of the soil organic carbon and total nitrogen. Application of both manures to the soil increased the EC values, but it was limited in the composted chicken manure.

Is biochar-manure co-compost a better solution for soil health improvement and N2O emissions mitigation?

Land application of compost has been a promising remediation strategy for soil health and environmental quality, but substantial emissions of greenhouse gases, especially N2O, need to be controlled during making and using compost of high N-load wastes, such as chicken manure. Biochar as a bulking agent for composting has been proposed as a novel approach to solve this issue, due to large surface area and porosity, and thus high ion exchange and adsorption capacity. Here, we compared the impacts of biochar-chicken manure co-compost (BM) and chicken manure compost (M) on soil biological properties and processes in a 120-d microcosm experiment at the soil moisture of 60% water-filled pore space. Our results showed that BM and M addition significantly enhanced soil total C and N, inorganic and KCl-extractable organic N, microbial biomass C and N, cellulase enzyme activity, abundance of N2O-producing bacteria and fungi, and gas emissions of N2O and CO2. However, compared to the M treatment, BM significantly reduced soil CO2 and N2O emissions by 35% and 27%, respectively, over the experimental period. The 15N-N2O site preference, i.e., difference between 15N-N2O in the center position (δ15Nα) and the end position (δ15Nβ), was ∼17‰ for M and ∼26‰ for BM during the first week of incubation, suggesting that BM suppressed N2O from bacterial denitrification and/or nitrifier denitrification. This inference was well aligned with the observation that soil glucosaminidase activity and nirK gene abundance were lower in BM than M treatment. Further, soil peroxidase activity was greater in BM than M treatment, implying soil organic C was more stable in BM treatment. Our data demonstrated that the biochar-chicken manure co-compost could substantially reduce soil N2O emissions compared to chicken manure compost, via controls on soil organic C stabilization and the activities of microbial functional groups, especially bacterial denitrifiers.

Phosphorus Extraction from Soil Constituents Using Bray P-1, Mehlich-1 and Olsen Solutions

The extraction methods using Bray P-1, Mehlich-1, and Olsen solutions are the most frequently used in equilibrium condition to estimate the available P in the soil constituents. However, each method can gives some different values that may not describe the availability of soil P. Therefore, it is necessary to conduct a laboratory experiment to compare the three solutions in equilibrium and kinetics conditions for P release from soil colloids as a basic data for the future related to plant productions. The objective of this study was to compare the amounts of P release in equilibrium and kinetics conditions using Bray P-1, Mehlich-1, and Olsen solutions and the rate constants (k) of P release from soil colloids using the three solutions of five soil constituents or treatments: (1) Soil (100% soil), (2) P-rock (100% phosphate rock), (3) compost (100% chicken manure compost), (4) soil + P-rock (75% soil + 25% phosphate rock), and (5) soil + P-rock + compost (50% soil + 25% phosphate rock + 25% chicken manure compost). Each extraction for each treatment was conducted in triplicate. The results indicate that the amounts of extracted P employing equilibrium condition in all treatments are significantly lower compared to those employing kinetics condition. The results also show that Mehlich-1 solution is the most powerful solution to extract P from soil constituents followed by Bray P-1 solution and Olsen solution, except that in the soil + phosphate rock + compost treatment in which the amount of P extracted using Mehlich-1 solution is the highest then followed by that of P extracted by Olsen solution and Bray P-1 solution both in equilibrium and kinetic conditions. The highest rate constants (k) of the reaction in all treatments were found in the extractions using Mehlich-1 solution for desorbed P followed by that of using Bray P-1 solution and Olsen solution. Keywords: Compost, equilibrium, kinetics, P extraction solutions, phosphate rock, soil

Effects of bamboo charcoal on antibiotic resistance genes during chicken manure composting

Composting is widely used for animal waste disposal, and bamboo charcoal (BC) can be used for nitrogen conservation during composting. However, the effects of BC on antibiotic resistance genes (ARGs) during chicken manure composting are still unclear. This study investigated the effects on ARGs of adding different proportions of BC (0%, 5%, 10%, and 20% w/w) to chicken manure compost. After 26 days, the relative abundances (RAs) of most ARGs (tetC, tetG, tetW, tetX, sul2, drfA1, drfA7, ermB, ermF, ermQ, and ermX) and intI1 declined by 21.6–99.5%, whereas sul1 increased by 7.5–17.7 times. The average RAs reductions with 0%, 5%, 10%, and 20% BC were 0.85, 1.05, 1.08, and 1.15 logs, respectively. The most important environmental factor for the ARG profiles was temperature according to redundancy analysis. Furthermore, BC significantly decreased the bio-Cu and bio-Zn levels, thereby reducing the co-selection pressure from heavy metals. Different proportions of BC had no significant effects on the removal of tetG, tetW, tetX, sul2, drfA1, and ermB. Supplementation with 10% BC was more effective at removing tetC and drfA7 compared with the other treatments. The results suggested that 10% BC supplementation is appropriate for reducing ARGs in chicken manure compost.