Chicken Intestine Research Articles

Comparative analysis of innate immune responses in Sonali and broiler chickens infected with tribasic H9N2 low pathogenic avian influenza virus

BackgroundH9N2 avian influenza viruses have been circulating in Bangladesh since 2006, affecting multiple avian species and resulting in economic losses. The recent emergence of tribasic strains, along with co-infections, has increased the risk to poultry health. Therefore, the study aimed to compare the immune responses of Sonali (crossbred) and commercial broiler chickens infected with tribasic H9N2 low pathogenic avian influenza (LPAI) virus.MethodsFollowing H9N2 infection, proinflammatory (IL-6, IL-8, IL-1β and TNF-α) and antiviral (IFN-β and IFN-γ) cytokine expressions were observed in the trachea, lungs, intestine, and lymphoid tissues in Sonali and broiler chickens from 1 day post infection (dpi) to 10 dpi by qPCR.ResultsSonali chickens exhibited significantly higher proinflammatory and antiviral cytokine expressions in the trachea at 3–7 days post infection (dpi), while broiler chickens showed lower immune responses. Broiler chickens displayed prolonged IL-6, IL-8, and IL-1β expression in lungs at 3–10 dpi compared to Sonali chickens. In the intestine, broiler chickens showed higher IL-6 and IL-8 expression that peaks at 1–3 dpi, while in Sonali chickens only IL-1β elevated at 10 dpi. In response to the H9N2 viruses, broiler chickens exhibited a stronger early IFN-β responses and a delayed IFN-γ responses in their lymphoid organs compared to Sonali chickens.ConclusionThis suggests distinct immune profiles between the chicken types in response to the H9N2 infection. The information sheds light on the function of innate immunity in the pathophysiology of currently circulating tribasic H9N2 virus and could assist in effective controlling of avian influenza virus spread in poultry and designing vaccines.

Isolation, Potential Beneficial Properties, and Assessment of Storage Stability of Direct-Fed Microbial Consortia from Wild-Type Chicken Intestine.

Direct-fed microorganisms (DFM) are recognized as an alternative to antibiotic-based growth promoters in poultry production due to their health benefits. DFM, however, should undergo rigorous safety testing to ensure they meet the criteria to be "Generally Recognized as Safe." This study assessed eight bacterial consortia (BC) isolated from the ileal and cecal intestinal regions of wild-type chickens, subjecting them to probiotic tests. Subsequently, they were spray- and freeze-dried to evaluate their storage stability for 30days. BC5-I and BC7-I, isolated from the ileum, emerged as promising DFM, displaying a high content of Lactobacillales using a selective medium and higher susceptibility to Gram-positive and Gram-negative antibiotics. These BC showed a high tolerance to temperature (> 90%), pH > 4 (88-98%), and antagonist effects against Escherichia coli and Salmonella. BC5-I exhibited superior survival in the simulated gastric conditions and satisfactory intestine mucus adhesion. Freeze-drying was the best method to obtain BC5-I and BC7-I powders, with a survival efficiency of 80.3% and 73.2%, respectively, compared to the beginning of storage. BC5-I presented the lowest cell death rate and prolonged half-life through survival storage kinetics. BC5-I only contained Lactobacillus, and Limosilactobacillus reuteri was the predominant species in liquid (78.3%) and freeze-dried (59.8%) forms. BC5-I stands out as a promising Lactobacillus-based DFM that could improve chicken intestinal health and enhance meat and egg production.

Waste to Energy: Combustion, Performance, and Emission Characteristics of Waste Animal Fats/Diesel Blends Premixed with Various Alcohols as Port Fuels

AbstractAnimal flesh is a major food source with economic and industrial value for consumer demand. These meats produced biowaste during and after preparation and use. Chicken intestines make up most of the waste thrown away after processing or frying. This study considers it a biodiesel source. Transesterification turns chicken intestine waste fat oil into biodiesel. This oil is used in compression ignition (CI) engines but performs poorly compared to diesel. Diesel, the base fuel, is mixed with 20% biodiesel. The remaining 10% and 20% of butanol and pentanol are port fuels, improving combustion and lowering emissions in the 5.2 kW, 1500 rpm CI engine. 20% pentanol premixing outperformed butanol premixing, blending, and engine CIWFOB operation. The greater heating value improves combustion, therefore 20% pentanol premixing with blend produces 32.76% BTE, 10.57% more than diesel. It produced 55.18% less CO and 50.92% less smoke than diesel, which has a greater heat release rate (48.86 J/CAD) and peak pressure (64.76 bar). This premixing costs NOx emissions. The CIWFOB blend with 20% pentanol premixing improves engine performance. For SDGs 7, 9, 12, and 13, this study is supported.

Assessing the Impact of Clove (Syzygium Aromaticum) Extract on Intestinal and Hepatic Histological Alterations in Broiler Chickens Infected with Salmonella typhimurium

This study investigated effects of aqueous and ethanolic clove extracts in small intestine and liver of chickens exposed to Salmonella typhimurium. Two hundred fifty-day-old chicks were divided into five groups. Group 1 served as the control and received a basic diet.Group 2 was orally infected with Salmonella typhimurium. Group 3 received same infection but was treated with Ciprofloxacin. Groups 4 and 5 were infected but received treatments of aqueous and ethanolic clove extracts, respectively. The findings revealed that Group 1 exhibited normal small intestine, while Group 2 displayed severe abnormalities, including flattened mucosa, damaged villi, and inflammation. Group 3 showed improvements, increased cell growth and goblet cells in the small intestine. Group 4 displayed nearly normal intestinal features. Group 5 had fully restored intestinal health. In the liver, Group 1 showed typical hepatic structure, whereas Group 2 exhibited signs of inflammation and hepatocyte necrosis. Group 3 displayed severe hepatocyte damage, while Group 4 demonstrated restored liver structure with slight sinusoidal dilation. Group 5 had a well-preserved hepatic architecture with minor inflammation. Administration of 2 mg/kg of aqueous and ethanolic clove extracts demonstrated alleviate histological alterations in small intestine and liver caused by Salmonella typhimurium infection. Notably, the aqueous extract is more effective than ethanolic extract in reducing liver and intestine damage.

Analyzing the morphology and avian β-defensins genes (AvβD) expression in the small intestine of Cobb500 broiler chicks fed with sodium butyrate

BackgroundSodium butyrate is a potential antibiotic growth promoter and has had advantageous effects on the poultry industry.MethodsEvaluating the effect of sodium butyrate on the intestinal villi and the humoral part of innate immunity of the male Cobb 500 broiler using scanning electron microscopy and quantitative real-time PCR analysis, the control group and treated group of Cobb 500 with SB supplemented received water containing 0.98 mg sodium butyrate.ResultsThe administration of sodium butyrate changed the villi characters, as the shape changed from tongue to long tongue. They were mainly parallel to each other and long finger-like at the duodenum. The tips of the villi in the control group appeared thin-slight curved with a prominent center in the duodenum, thin rectangular in the jejunum, and ileum in the control group. In contrast, in the treatment group, they changed to thick rectangular in the duodenum and ileum zigzag shape in the jejunum. The epithelium lining of the duodenal villi showed a dome shape, the jejunal villi showed a polygonal shape, and the ileal villi appeared scales-like. The epithelium lining showed irregular microfolds and many different-sized pores, and the treatment group showed islands of long microvilli in the duodenum and solitary long microvilli in the ileum. Real-time PCR of AvBD 1, 2, 10, and 12 significantly (P < 0.01). The better expression of AvBD 1, 2, and 12 was determined in the duodenum, while AvBD 10 was in the jejunum.ConclusionSodium butyrate enhanced the chicks’ growth and small intestine parameters, modified the morphology of the intestinal villi, and improved the humoral part of innate immunity.

Evaluation of in vitro probiotic properties and colonization of Brevibacillus Laterosporus S62-9 in the intestine of broiler chickens

Brevibacillus laterosporus has been used as a direct-fed microbial additive in chicken feed owing to its strain safety and the ability to produce broad-spectrum antimicrobial peptides. To assess the efficacy and persistence of Brevibacillus laterosporus, it is necessary to track the supplied strains throughout the gastrointestinal tract. This study aims to evaluate the in vitro probiotic properties of Brevibacillus laterosporus S62-9 and its capacity to colonize and persist in the intestines of broiler chickens. Results indicated that the spores of Brevibacillus laterosporus S62-9 exhibited higher tolerance to the digestive condition, and higher adhesion rate to mucus and intestinal cells compared to vegetative cells. The spores remained dormant during digestion in the crop and stomach but germinate in the small intestine with a germination rate exceeding 70%. Subsequently, the whole genome of S62-9 was sequenced, and specific primers were designed and validated based on comparative genome analysis. The quantitative PCR-based method had a detection limit ranging from 2.71 to 7.71 log10 CFU/mL in pure culture and from 3.25 to 9.25 log10 CFU/g in broiler cecal content. In vivo colonization assay indicated that the Brevibacillus laterosporus S62-9 can transiently colonize the broiler cecum. In summary, our results indicated that the Brevibacillus laterosporus S62-9 spores exhibited high tolerance to the digestive tract environment, germinated in the intestine, and establish colonization in the cecum of broilers. The findings provide a theoretical basis for the future application of Brevibacillus laterosporus S62-9 in broiler farming.

Effects of probiotic Subalin on body weight gain in broiler chickens with spontaneous eimeriosis

The purpose of the research is to study the probiotic Subalin effects on body weight gain in broiler chickens with spontaneous eimeriosis.Materials and methods. On a poultry farm in the Moscow Region, the body weight gain dynamics was studied in 120 floormanaged broiler chickens aged 9 to 40 days spontaneously infected with eimeria after administration of different doses of the probiotic Subalin vs. control. The chickens selected for the experiment were divided into four similar groups of 30 birds each. Subalin was administered to the first group chickens at a dose of 1.5 mL per 1 L of drinking water, while the broilers of the second and third groups were administered Subalin at a dose of 2 and 2.5 mL per 1 L of water, respectively. Subalin doses were given to the chickens of groups 1–3 in three stages: on 11–12; 18–19 and 26–27 days of age for consecutive 48 hours. The broilers of group 4 did not receive the drug and served as a control. The chickens of all groups underwent clinical, hematological, and coprological examinations; they were kept in similar conditions on the floor, in isolation, and fed as per zootechnical standards. Control weighing of the chickens was done on days 10 and 40 of their growth.Results and discussion. The total weight gain of the group 1 chickens was 1985 g in the experiment; 2203 g and 2214 g in groups 2 and 3, respectively, and 1803 g in the control group. The productivity of the experimental chickens was 10.1; 22.2 and 22.8% higher vs. control. The Subalin optimal dose was 2 mL per 1 L of drinking water according to the above scheme. The tested doses of Subalin had positive effects on the number of enterobacteria in the chicken intestines. Blood tests showed no statistically significant changes in clinical or hematological parameters in the experimental and control chickens.

The Occurrence of Colistin Resistance in Potential Lactic Acid Bacteria of Food-Producing Animals in India.

The overuse of colistin, the last-resort antibiotic, has led to the emergence of colistin-resistant bacteria, which is a major concern. Lactic acid bacteria which are generally regarded as safe are known to be reservoirs of antibiotic resistance that possibly pose a threat to human and animal health. Therefore, this study assessed the prevalence of colistin antimicrobial resistance in livestock in India, that is lactic acid bacteria in healthy chickens, sheep, beef, and swine of Mysore. Diverse phenotypic and genotypic colistin resistance were examined among the lactic acid bacterial species (n = 84) isolated from chicken (n = 44), sheep (n = 16), beef (n = 14), and swine (n = 10). Hi-comb, double-disk diffusion tests, Minimum Inhibitory Concentration (MIC), and biofilm formation were assessed for phenotypic colistin resistance. Specific primers for colistin-resistant genes were used for the determination of genotypic colistin resistance. Around 20%, 18%, and 1% were colistin-resistant Lactobacillus, Enterococcus, and Pediococcus species, respectively. Among these, 66.67% exhibited MDR phenotypes, including colistin antibiotic. The identified resistant isolates are Levilactobacillus brevis LBA and LBB (2), Limosilactobacillus fermentum LBF (1), and Pediococcus acidilactici CHBI (1). The mcr-1 and mcr-3 genes were detected in Levilactobacillus brevis LBA, LBB, and Pediococcus acidilactici CHBI isolated from chicken and sheep intestines respectively. The study identified colistin resistance determinants in lactobacilli from food animals, emphasizing the need for enhanced surveillance and monitoring of resistance spread. These findings underscore colistin resistance as a significant medical concern and should be integrated into India's ongoing antimicrobial resistance monitoring programs.

Hox gene activity directs physical forces to differentially shape chick small and large intestinal epithelia.

Hox transcription factors play crucial roles in organizing developmental patterning across metazoa, but how these factors trigger regional morphogenesis has largely remained a mystery. In the developing gut, Hox genes help demarcate identities of intestinal subregions early in embryogenesis, which ultimately leads to their specialization in both form and function. Although the midgut forms villi, the hindgut develops sulci that resolve into heterogeneous outgrowths. Combining mechanical measurements of the embryonic chick intestine and mathematical modeling, we demonstrate that the posterior Hox gene HOXD13 regulates biophysical phenomena that shape the hindgut lumen. We further show that HOXD13 acts through the transforming growth factor β (TGF-β) pathway to thicken, stiffen, and promote isotropic growth of the subepithelial mesenchyme-together, these features lead to hindgut-specific surface buckling. TGF-β, in turn, promotes collagen deposition to affect mesenchymal geometry and growth. We thus identify a cascade of events downstream of positional identity that direct posterior intestinal morphogenesis.

Measurement of temperature, pH and substrate levels on the activity of the Galactose-1-Phosphate Uridyltransferase enzyme

Galactose-1-phosphate uridyltransferase (GALT) is an important enzyme involved in galactose metabolism. Understanding the factors influencing GALT activity is critical to elucidate its physiological role and potential therapeutic implications in galactosemia. In developing new drugs, chicken intestine/liver powder can be used as an enzyme source, GALT, to treat galactosemia. Therefore, it is necessary to research the characterization of the GALT enzyme in chicken intestine and liver powder. In this study, we investigated the influence of temperature, pH, and substrate level on GALT enzyme activity using an experimental approach in vitro. The optimum pH extraction results show that the optimum pH for the extraction of the chicken intestine and liver GALT is pH 7, with activity values ​​of 0.47 units/mL and 0.3953 units/mL, respectively. The optimum temperature for the extraction of chicken intestine and liver GALT is 37℃ with substrate hydrolysis capabilities of 0.48 U/mL and 0.57 U/mL, respectively. Meanwhile, the optimum substrate content is 400x. These insights provide a valuable foundation for further research aimed at comprehensively understanding GALT function, developing targeted interventions for disorders of galactose metabolism, and possible application in the development of new drugs for galactosemia

Isolation of Bacillus licheniformis and its protective effect on liver oxidative stress and apoptosis induced by aflatoxin B1

Aflatoxin B1 (AFB1) is one of the most toxic mycotoxins. The use of probiotics is an effective approach to reduce aflatoxins content in foods. To find efficient bacterial species that can eliminate or detoxify AFB1, a bacterial strain S51 capable of degrading AFB1 was isolated from chicken intestine and soil samples by using a culture medium containing coumarin as the sole carbon source. Based on the results of 16S rRNA gene sequence analysis, this isolate (strain S51) was identified as Bacillus licheniformis strain QT338. Further characterization of strain S51 showed that it could degrade AFB1 by 61.3% after incubation at 30°C for 72 h. Additional studies demonstrated that S51 promoted good growth performance of the treated chickens, showed no hemolytic activity, carried few drug resistance genes, and exhibited a certain level of tolerance to acid and bile salts. Furthermore, to verify whether strain S51 exerts a protective effect on AFB1-induced liver injury in chickens and to elucidate the underlying mechanism, a chicken toxicity model was induced with AFB1 (100 μg/kg BW) and treated with S51(1×109CFU/mL) for 12 d. The results showed that S51 decreased the level of alanine transaminase, aspartate transaminase, and total bilirubin (P < 0.05); increased glutathione activity and total antioxidant capacityin the liver induced by AFB1, and decreased malondialdehyde production (P < 0.05). S51 also up-regulated the mRNA expression level of the antioxidant proteins HO-1 and Nrf2 and down-regulated the expression of the oxidation-related factor Keap1 in the Nrf2/Keap1 signaling pathway (P <0.05). S51 inhibited hepatocyte apoptosis induced by AFB1 and decreased the mRNA expression levels of the apoptosis-related genes Bax, caspase-3, caspase-9, and Cyt-C (P < 0.05). These results indicate that S51 regulates apoptosis and alleviates AFB1-induced oxidative stress in chicken liver by controlling the Nrf2/Keap1 signaling pathway.

VirBR, a transcription regulator, promotes IncX3 plasmid transmission, and persistence of blaNDM-5 in zoonotic bacteria

IncX3 plasmids carrying the New Delhi metallo-β-lactamase-encoding gene, blaNDM-5, are rapidly spreading globally in both humans and animals. Given that carbapenems are listed on the WHO AWaRe watch group and are prohibited for use in animals, the drivers for the successful dissemination of Carbapenem-Resistant Enterobacterales (CRE) carrying blaNDM-5-IncX3 plasmids still remain unknown. We observe that E. coli carrying blaNDM-5-IncX3 can persist in chicken intestines either under the administration of amoxicillin, one of the largest veterinary β-lactams used in livestock, or without any antibiotic pressure. We therefore characterise the blaNDM-5-IncX3 plasmid and identify a transcription regulator, VirBR, that binds to the promoter of the regulator gene actX enhancing the transcription of Type IV secretion systems (T4SS); thereby, promoting conjugation of IncX3 plasmids, increasing pili adhesion capacity and enhancing the colonisation of blaNDM-5-IncX3 transconjugants in animal digestive tracts. Our mechanistic and in-vivo studies identify VirBR as a major factor in the successful spread of blaNDM-5-IncX3 across one-health AMR sectors. Furthermore, VirBR enhances the plasmid conjugation and T4SS expression by the presence of copper and zinc ions, thereby having profound ramifications on the use of universal animal feeds.

Effect of Triticale Grain in Diets on Performance, Development of Gastrointestinal Tract and Microflora in Crop and Ileum of Broiler Chickens.

The purpose of the research was to determine the effect of the use of a diet containing 30% triticale grain. In an experiment lasting 28 days, 180 one-day Ross-308 chickens (sex ratio 1:1) with an average initial body weight in treatment of 44.6 g were randomly assigned to 30 metabolic cages/replications, 6 birds in each. To compare the results between treatments, a one-way ANOVA was used with uneven replication numbers. The control group (I) received a standard diet containing maize and soybean meal. In the other treatments, 30% of different cereals were used: II-wheat, III-barley, and IV-triticale. Significant differences in body weight (BW) and feed conversion ratio (FCR) were observed on the 4th day of the life of broiler chickens (p < 0.05). Differences were determined between the control group (90.7 g BW and 1.32 kg of feed/kg BWG in the case of FCR) and birds receiving barley (93.0 g BW and 1.29 kg of feed/kg BWG in the case of FCR), compared to chickens fed diets with a 30% share of wheat grain (86.2 g BW and 1.53 kg feed/kg BWG in the case of FCR) and triticale (86.6 g BW and 1.53 kg feed/kg BWG in the case of FCR). Later, the differences in performance of birds between treatments did not occur (p > 0.05). In the nutrition of broiler chickens, control or 30% of the triticale diet caused a significant reduction (p < 0.01) of the number of Escherichia coli (E. coli) in the crop of broiler chickens (0 log cfu/g), compared to birds obtaining feed with 30% of wheat (1.78 log cfu/g). The diet containing triticale also reduced the number of E. coli (p < 0.05) within the ileum (0.78 log cfu/g) compared to chickens obtaining barley grain in the diet (2.12 log cfu/g). As a result of the use of triticale grain (p < 0.05), the total length of the bird intestines (199.64 cm) was compared to 30% of barley grain (209.76 cm). The increase in the length of the large intestine of broiler chickens in treatments was positively correlated (r = 0.613, p < 0.05) with the number of Lactobacillus sp. in the ileum. Triticale increased the pH in the crop of broilers chickens. The research results indicate that triticale, after longer storage, can be used in amounts of 30% of the diet without significant effect on the performance of broiler chickens, with a reduction in E. coli in crop in comparison with wheat and in ileum with barley.

Benefit and potential risk: Effects of in ovo copper oxide nanoparticles supplementation on hatchability traits, organ weightsand histological features of newly hatched chicks.

This investigation was directed to examine the influence of copper oxide nanoparticles (CuO-NPs) on the hatchability traits, and chick quality of newly hatched broiler chicks. A total of 480 eggs were randomly divided into four treatment groups, each consisting of three duplicates. As a negative control (NC), the first group was not injected; the second group was injected with saline and served as a positive control (PC), the third and fourth groups were injected with 30 and 60 ppm of (CuO-NPs)/egg. Eggs were injected into the amniotic fluid on the eighteenth day of the incubation period. Results showed that the hatchability, chick yield %, yolk free-body mass (YFBM), chick length, shank length (SL), and relative weight of the heart, gizzard and intestine of day-old broiler chicks were all unaffected by the in ovo injection of CuO-NPs. The Pasgar Score was slightly improved compared to the NC and PC groups. Also, the in ovo administration of CuO-NPs (60 ppm/egg) significantly increased the intestine length. Both levels of CuO-NPs significantly increased the concentration of Cu ions in the hepatic tissue. Additionally, different levels of tissue damage were seen in the liver of the birds that were given low or high dosages of CuO-NPs. Conclusively, the in ovo injection of CuO-NPs has a good result on the appearance of the chicks (Pasgar score). However, negative effect of CuO-NPs on liver tissue may raise concerns about the potential risks of applying CuO-NPs in ovo administration.

Genomic tailoring of autogenous poultry vaccines to reduce Campylobacter from farm to fork

Campylobacter is a leading cause of food-borne gastroenteritis worldwide, linked to the consumption of contaminated poultry meat. Targeting this pathogen at source, vaccines for poultry can provide short-term caecal reductions in Campylobacter numbers in the chicken intestine. However, this approach is unlikely to reduce Campylobacter in the food chain or human incidence. This is likely as vaccines typically target only a subset of the high genomic strain diversity circulating among chicken flocks, and rapid evolution diminishes vaccine efficacy over time. To address this, we used a genomic approach to develop a whole-cell autogenous vaccine targeting isolates harbouring genes linked to survival outside of the host. We hyper-immunised a whole major UK breeder farm to passively target offspring colonisation using maternally-derived antibody. Monitoring progeny, broiler flocks revealed a near-complete shift in the post-vaccination Campylobacter population with an ~50% reduction in isolates harbouring extra-intestinal survival genes and a significant reduction of Campylobacter cells surviving on the surface of meat. Based on these findings, we developed a logistic regression model that predicted that vaccine efficacy could be extended to target 65% of a population of clinically relevant strains. Immuno-manipulation of poultry microbiomes towards less harmful commensal isolates by competitive exclusion, has major potential for reducing pathogens in the food production chain.

Fecal microbiota transplantation provides insights into the consequences of transcriptome profiles and cell energy in response to circadian misalignment of chickens

The circadian misalignment (CM) disordered circadian rhythms exert adverse effects on animals. Poultry as one of animals suffers health and welfare problems due to long-term lighting photoperiods caused by CM. However, the roles of CM on organ development, cell growth, metabolism and immune are still unclear in chickens. In this study, a Chinese dual-purpose native breed, was used to explore the effects of CM on transcriptomic pattern of brain and cell energy biogenesis, and further fecal microbiota transplantation (FMT) was applied to investigate its "therapy" effect from CM suffering. Our results showed that the CM led to stunting in brain and small intestine of chicken. CM decreased of cell proliferation, and energy production, mtDNA copies and expression of genes related to cell cycle or mitochondrial biogenetics, while it upregulated the reactive oxygen species (ROS) level and the sensitivity to inflammation. Interestingly, FMT rescued the organ developmental defects and cell dysfunctions induced by CM. Circadian misalignment brought about abnormal tissue and cell developments, energy biogenesis, and immune response in birds. This study provided a comprehensive perspective on understanding the regulation of CM and FMT on bird development and welfare.

The Utilization of Processing Waste (Chicken Intestines and Shrimp Heads) as Alternative Bait in Blue Swimming Crab Fishing in Betahwalang, Demak, Indonesia

The Utilization of Processing Waste (Chicken Intestines and Shrimp Heads) as Alternative Bait in Blue Swimming Crab Fishing in Betahwalang, Demak, Indonesia

THE INFLUENCE OF GREEN MARKETING AND DIGITAL MARKETING ON CUSTOMER LOYALTY IN CHICKEN INTESTINE CHIPS MSMEs IN KEDIRI

The problem addressed in this research concerns marketing management, focusing on the impact of green marketing and digital marketing on customer loyalty. The objective is to determine and analyze the effects of green marketing and digital marketing on customer loyalty both individually and collectively. This study uses a quantitative approach, with a population consisting of customers of chicken intestine chips MSMEs in Kediri. The sample includes 85 respondents. Data collection was conducted using questionnaires, and data analysis involved validity, reliability, classical assumption tests, multiple linear regression tests, and hypothesis testing. The research hypotheses testing revealed that green marketing has a significant partial effect on customer loyalty, digital marketing also has a significant partial effect on customer loyalty, and both green marketing and digital marketing have a significant simultaneous effect on customer loyalty in chicken intestine chips MSMEs in Kediri. Overall, this study contributes to the existing body of knowledge in marketing management by shedding light on the effects of green marketing and digital marketing on customer loyalty. The findings suggest that implementing these strategies can be beneficial for MSMEs in the chicken intestine chips industry in Kediri, as they can enhance customer loyalty and potentially lead to increased business success.

Growth Performance of Crocodile Fed with Chicken Intestine and Trash Fish at Smallholder Farm in Siem Reap Province, Cambodia

Growth Performance of Crocodile Fed with Chicken Intestine and Trash Fish at Smallholder Farm in Siem Reap Province, Cambodia

NEWCASTLE DISEASE AND AVIAN INFLUENZA IN BROILER THAT VACCINATED ON ONE DAY OLD

Newcastle disease and Avian Influenza are highly contagious viral diseases that can affect poultry with high mortality rates, and are zoonotic diseases that endanger humans. In this case study, broiler chickens aged 12 days were vaccinated at hatching and came from Yangapi Village, Tembuku, Bangli showed clinical symptoms of drooping neck or torticollis, no appetite, and greenish white diluted feces. Examinations are continued at each laboratory to obtain a definite diagnosis. The methods used are anatomical pathology examination, histopathology preparations, HA-HI examination, bacterial identification, and qualitative examination for paracites. The results of anatomical pathology examination found pathognomonic lesions, like ptechie in the proventriculus and ventriculus, enlargement and congestion in the lungs, hemorrhagic trachea, and hyperemic intestines. The results of histopathological examination showed marked changes with single-core inflammatory cells or lymphoid suggesting an infectious agent in the form of a virus. The results of the virology laboratory examination with the HA and HI test showed that the case chicken was positively infected by Newcastle Disease and Avian Influenza viruses. Bacteriology laboratory by culture of lung and intestinal organ samples was carried out on Nutrient Agar, followed by selective media Eosin Methylene Blue Agar, primary tests, gram staining, biochemical tests and sugar tests until the results showed that Escherichia coli bacteria grew from the chicken intestines and were classified as normal flora. The results of the examination related to parasites in the feces of case chickens by qualitative methods, natif, sedimentation concentration, and floating were confirmed not to be found worm eggs or coccidia. From this case study, it can be concluded that the case chicken was infected with Newcastle Disease and Avian Influenza viruses.