Chicken Farms Research Articles

27. Working time in broiler chicken and turkey farming

27. Working time in broiler chicken and turkey farming

Distribution of extended-spectrum β-lactamase producing Escherichia coli genes in an integrated poultry-fish farming system in Bogor, Indonesia.

The excessive use of antimicrobials in livestock farming leads to the emergence and dissemination of antimicrobial-resistant organisms. This study aimed to detect extended-spectrum β-lactamase (ESBL)-producing Escherichia coli genes in integrated poultry-fish farms in Bogor, Indonesia. A total of 256 samples were collected from six poultry-fish farms. One hundred and seventy-five chicken cloaca swabs, 60 fish skin swabs, six pond water samples, and 15 farmer's hand swabs. ESBL-producing E. coli was confirmed through double-disk diffusion. The specific primers and probe genes for quantitative polymerase chain reaction detection of ESBL-producing E. coli targeted blaTEM, blaCTX-M, blaSHV, and blaOXA-48 genes. Among the 256 samples tested, 145 (56.6%) were positive for E. coli, and 67.6% (98/145) were identified as ESBL-producing E. coli. The most ESBL-producing E. coli isolates were obtained from chicken cloaca (78.3%, 72/92), followed by pond water (66.7%, 4/6), fish skin (47.6%, 20/42), and farmer's hand swabs (40%, 2/5). About 100% of the isolates carried the genes blaTEM and blaCTX-M, whereas 17.3% and 24.5% carried blaSHV and blaOXA-48, respectively. ESBL-producing E. coli genes were investigated in chicken cloaca, fish, pond water, and farmers' hands within an interconnected poultry-fish farming operation. The ESBL-producing E. coli in chickens can transfer resistant genes to aquatic environments. The transfer could harm other aquatic species and food chains, potentially threatening human health.

Evaluation of Productivity and Financial Feasibility of Broiler Chicken Farming Business Models: Cage and Postal Systems

The growth of the human population and increased purchasing power drive greater demand for broiler meat, posing challenges for farmers to enhance their productivity and business efficiency. The development of closed-house cages offers a solution to improve production efficiency. This study compares the productivity and financial analysis between the cage and postal models. Utilizing a quantitative descriptive method, the research was conducted over a single rearing period in cages and postal setups at PT Dinamika Megatama Citra (DMC) without any specific treatment. Research parameters encompassed body weight gain, mortality, FCR, harvest weight, and Performance Index. Financial analysis was conducted using indicators such as the R/C ratio, B/C ratio, Break-Even Point (BEP), and Return on Investment (ROI). Results indicate that in cage conditions, mortality stood at 4.07%, FCR at 1.718, average weight at 2.2 kg, and Performance Index at 287.5. Meanwhile, postal cages exhibited higher mortality at 8.79%, FCR at 1.732, average weight at 2.3 kg, and Performance Index at 285.6. In financial analysis, cage systems had a BEP price of Rp 19,726/kg with a unit BEP of 40,616 kg, an R/C ratio of 1.039, a B/C ratio of 0.039, and an ROI of 3.92%. Conversely, postal cages had a BEP price of Rp 19,507/kg with a unit BEP of 39,924 kg, an R/C ratio of 1.05, a B/C ratio of 0.05, and an ROI of 5.09%.

An efficient transformer network for detecting multi-scale chicken in complex free-range farming environments via improved RT-DETR

Accurate detection and localization of chicken targets in complex environments are the foundation of intelligent free-range chicken farming. However, commercial free-range chicken farming poses several challenges to detection methods, including complex and variable backgrounds, multi-scale targets, aggregated occlusion and changes in the posture of chicken. While there has been significant research on object detection algorithms based on convolutional neural networks, these algorithms have limited effectiveness in detecting small targets and generalizing to new scenes. Therefore, this study proposes a highly accurate and strongly generalizable chicken object detection model called Efficient Multi-scale Chicken DETR (EMSC-DETR), which is based on an improved Real-Time DEtection TRansformer (RT-DETR). To address the issue of easily losing small target features, a new efficient transformer module called space-to-depth transformer module (SDTM) is introduced. SDTM includes space-to-depth (SPD) and BiFormer modules, which facilitate deep interaction between local and global features, and significantly improve the computational efficiency of the transformer. Additionally, to overcome the detection and differentiation difficulties caused by chicken aggregation occlusion, the transformer encoder of EMSC-DETR incorporates a contextual transformer (CoT). CoT utilizes rich contextual information among the nearest neighbors to enhance the feature representation of high-level features, enabling more accurate localization of overlapped chicken targets. To evaluate the proposed model in complex environments, 14 scene data were collected from two fields and divided into training, validation, testing sets and three independent testing sets with unknown scenes. Experimental results show that the proposed EMSC-DETR obtains the best detection performance in the testing set, with an AP50 of 98.6%, surpassing other state-of-the-art models of similar size. In the three independent testing sets, the proposed model shows improvements of 8.2%, 9.2% and 28.3% in the AP50 compared to the original RT-DETR, respectively. Moreover, the model exhibits superior generalization ability for unknown scenes compared to other state-of-the-art models. Robustness and fine-tuning experiments further confirm the reliability and application potential of EMSC-DETR in detecting free-range chickens, which is crucial for complex commercial farming. This study is expected to provide references and new ideas for precision livestock farming.

Assessment of Local Chicken Production Base Area in Minahasa Regency: A Location Quotient Approach

Local chickens have considerable potential to be developed and are reared as a source of animal protein and income. The production of local chickens is distributed across Minahasa Regency under a traditional free-ranging system. This study aims to assess the base area of local chicken farming and to determine the distribution of the superior commodity through the growth and share of local chicken production. Assessing the base area of the region for local chicken production can serve as the basis for the evaluation and design of policies. This study utilized a location quotient (LQ) approach in the empirical works in the regency and district. This analytical tool was applied to analyze the dynamics of the economic base in the cases of districts in Minahasa Regency, Indonesia. The analysis results denoted that 17 of 25 districts in Minahasa Regency ranked among the top base areas for local chicken development, as indicated by the highest LQ coefficients. Concerning the district potential base area, three districts fell into the superior category, four districts belonged to the static category, and 15 districts were in the potential category. This evidence reflects the potential and prospects of local chicken farming and its development plans as a leading commodity in Minahasa Regency. Encouraging farmers to maintain and develop livestock enterprises could support the provision of animal protein for consumption. There has been hope for the future of the local chicken commodity thanks to its inclusive development in resource-rich base areas.

Penerapan Limbah Nenas Fermentasi Untuk Pengembangan Ayam Kampung Di Desa Tangkit Baru Kabupaten Muaro Jambi

The objectives of this community service (PPM) are as follows: (1) the application of pineapple waste fermentation technology with Trichoderma viride fungi for kampung chicken feed, (2) improving the knowledge and skills of poultry farmers in formulating cost-effective rations, (3) enhancing the knowledge and skills of poultry farmers in more efficient management and better production. The implementation method of community service involves active interaction between the project team and the target groups, which include 40 community members and farmer groups. The key stages in the implementation of the PPM are as follows: (1) dissemination and training, (2) active participation of farmer group members with the establishment of a Technical Consultation Service (TCS) forum for consultation and dialogue between the project team and poultry farmers, (3) hands-on practice in kampung chicken production management and pineapple waste processing using fermentation technology, (4) monitoring and evaluation (Monev) of the community service program in the farmer groups of Tangkit Baru, Muaro Jambi. The results of the community service activities are as follows: (1) the dissemination and training sessions have successfully increased the knowledge and skills of the participants, with the dissemination being rated as good, (2) the application of fermented pineapple waste as kampung chicken feed has been well-received by the farmer groups and the community in Tangkit Baru, Muaro Jambi (3) the implementation of this PPM program has proven highly beneficial for poultry farmers and implementing institutions, as it serves as a platform for the transfer of knowledge and technology, particularly in the traditional and non-business-oriented kampung chicken farming practices, thus optimizing the outcomes of these practices and facilitating the development of kampung chicken farming businesses.

Autonomous navigation using machine vision and self-designed fiducial marker in a commercial chicken farming house

Indoor navigation within commercial animal farming houses is crucial for the increasing operations carried out by mobile robots. However, existing methods often suffer from issues such as sensitivity to ambient light, insufficient accuracy for precise animal operations, or complexity in implementation. In this study, we proposed a novel navigation method for mobile chassis in a commercial stacked cage farming house, utilizing an active light source, self-designed fiducial marker, and machine vision. Our navigation system incorporated imaging modules equipped with 850 nm infrared light to minimize animal stress and mitigate the effects of low illumination. The self-designed fiducial marker, DRITag, enabled precise maker identification with a recognition rate of 100 % and achieved high positioning accuracy, with an absolute mean error of 0.34 cm within a range of 1.2 m. To determine the chassis’ yaw angle, we employed the U-net deep learning network, which achieved satisfactory root mean square errors of 0.22° and 0.10° on the ground and upper floors, respectively. Global localization of the chassis across different farm areas was achieved through a combination of DRITag and odometry information. Our proposed method provided a flexible and user-friendly approach to navigation path planning, employing a waypoint task text file that enabled easy modification of the path through simple text file editing. Field tests conducted in a two-story commercial stacked cage chicken farming house demonstrated the high accuracy of our navigation method, with standard deviations of longitudinal deviations below 1.85 cm and 1.82 cm, and standard deviations of lateral deviations below 1.50 cm and 1.12 cm at a speed of below 0.3 m/s on the ground floor and at a speed of 0.2 m/s on the upper floor, respectively. Overall, our study presented an independent-of-ambient-light, highly accurate, and easily implementable navigation method for mobile chassis in commercial chicken farming houses, with significant potential for future research in mobile robotics within animal farming environments.

Analisis Kelayakan Usaha Peternakan Ayam Petelur di Kecamatan Mantup Kabupaten Lamongan

The purpose of this study is to examine the profitability, income, and viability of laying hen farms in Lamongan Regency's Mantup District. Lamongan Regency's Mantup District. There were six informants who owned laying hen farms for this research. In this study, total costs, revenues, income, and business viability were the data analysis types used. The average total cost from six informants of IDR 755,812,161/Production Period is the result of this study. IDR 1,581,968,000 is the average revenue received per production period. During the production period, the average income obtained was IDR 826,155,833. Six laying hen breeder informants in Mantup District, Lamongan Regency, had their three business feasibility metrics calculated. The average R/C ratio value was 2.07, the average B/C ratio was 1.07, and the average ROI value was 92.50%. Therefore, it is viable to operate and grow the laying chicken farming company in Mantup District, Lamongan Regency, according to the calculation results.

Implementation of the WHO Tricycle protocol for surveillance of extended-spectrum β-lactamase producing Escherichia coli in humans, chickens, and the environment in Madagascar: a prospective genomic epidemiology study

Antimicrobial resistance (AMR) is a major public health threat, affecting not only people but also animals and the environment. The One Health dimension of AMR is well known; however, data are lacking on the circulation of resistance-conferring genes, particularly in low-income countries. In 2017, WHO proposed a protocol called Tricycle, focusing on extended-spectrum β-lactamase (ESBL)-Escherichia coli surveillance in the three sectors (humans, animals, and the environment). We implemented Tricycle in Madagascar to assess ESBL-E coli prevalence and describe intrasector and intersector circulation of ESBL-E coli and plasmids. In this prospective study, we collected blood culture data from hospitalised patients with a suspected bloodstream infection processed from May 1, 2018, to April 30, 2019, and rectal swabs from healthy pregnant women from July 30, 2018, to April 27, 2019, both from three hospitals in Antananarivo, Madagascar; and caeca from farm chickens and surface waters from the Ikopa river, wastewater, and slaughterhouse effluents in the Antananarivo area, Madagascar, from April 9, 2018, to April 30, 2019. All samples were tested for ESBL-E coli. The genomes of all isolates were sequenced using a short-read method on NextSeq 500 and NovaSeq 6000 platforms (Illumina, San Diego, CA, USA) and those carrying plasmid replicons using an additional long-read method on a MinION platform (Oxford Nanopore Technologies, Oxford, UK). We characterised genomes of isolated strains (sequence type, resistance and virulence gene content, and plasmid replicons). We then compared isolates using the variant calling method (single-nucleotide polymorphism). Data from 1056 blood cultures were collected and 289 pregnant women, 246 chickens, and 28 surface waters were sampled. Of the blood cultures, 18 contained E coli, of which seven (39%) were ESBL. ESBL-E coli was present in samples from 86 (30%) of 289 pregnant women, 140 (57%) of 246 chickens, and 28 (100%) of 28 surface water samples. The wet season (November to April) was associated with higher rates of carriage in humans (odds ratio 3·08[1·81-5·27]) and chickens (2·79 [1·65-4·81]). Sequencing of 277 non-duplicated isolates (82 from pregnant women, 118from chickens, and 77 from environmental samples) showed high genetic diversity (90 sequence types identified) with sector-specific genomic features. Single nucleotide polymorphism (SNP) analysis revealed that 169(61%) of 277 isolates grouped into 44 clusters (two or more isolates) of closely related isolates (<40 SNPs), of which 24 clusters contained isolates from two sectors and five contained isolates from all three sectors. ESBL genes were all blaCTX-M variants (215 [78%] of 277 being blaCTX-M-15) and were located on a plasmid in 113 (41%) of 277isolates. These ESBL-carrying plasmids were mainly IncF (63 [55%] of 114; one strain carried two plasmids) and IncY (42 [37%] of 114). The F31/36:A4:B1 (n=13) and F-:A-:B53 (n=8) pMLST subtypes, and the IncY plasmids, which were all highly conserved, were observed in isolates of differing genetic backgrounds from all sectors and were transferable invitro by conjugation. Despite sector-specific population structures, both ESBL-E coli strains and plasmids are circulating among humans, chickens, and the environment in Antananarivo, Madagascar. The Tricycle protocol can be implemented in a low-income country and represents a powerful tool for investigating dissemination of AMR from a One Health perspective. Fondation Mérieux and INSERM, Université Paris Cité.

Chemical Characteristics of Chicken Litter Waste in Closed-House System

Waste from the broiler and layer farming industry with a closed-house system continues to increase without optimizing waste utilization and harms the environment. Potential chicken litter waste from the chicken farming industry in West Sumatra is 5 tons per harvest (40 days) from a chicken livestock capacity of 100,000 chickens. This research aims to assess the potential and utilization and study the biochemistry of chicken manure waste in closed-house systems as biosorbents and fertilizers through amelioration technology. Closed-house chicken coop bedding waste (CHCCW) in the form of sawdust has functional groups such as carboxyl that can absorb cations because it can increase the negative charge in the soil so that it can be utilized by plants. In addition, the CHCCW can also absorb cations (pollutants). Chemical characteristics from the analysis results prove the ability of the CHCCW. Chicken litter waste has chemical characteristics that have the potential as a biosorbent and are valid as fertilizer, which has a proximate composition (moisture 4.26%; volatile matter 74.20%; ash 6.78% and fixed carbon 14.76%); pH (pH H2O 8.37 and pH PZC 7.37); electrical conductivity (EC) &gt;2 dS m-1 and cation exchange capacity (CEC) 182.67 Cmol(+)kg-1. The nutrient composition of chicken manure waste in closed-house systems has macro nutrients (6.88% C; 0.06% N; 5.89% P; 34.89% K; 36.28% Ca; 5.76% S) and micronutrients (2.49% Fe; 1.39% Mn; 1.22% Zn; 1.01% Cu; and 5.15% Cl). Chicken manure waste in closed-house systems also has functional groups such as O-H, N-H, C-H, C-OH, C=C, C=O, C-O-C, Si-O, and O-CH3, which play an active role in the absorption of pollutants and nutrients in the soil.

Access to credit and its effect on poultry farmers’ productivity: A case study of layer chicken farmers in Ikorodu Local Government Area of Lagos State, Nigeria

Despite the relevance of the poultry sub-sector to the economy of Nigeria and the capital-intensive nature of poultry production, many poultry farmers are unable to access adequate credit which affects their productivity. This study was therefore conducted to understand the effect access to credit has on farmers’ productivity, with emphasis on layer producers in Ikorodu Local Government Area of Lagos State, Nigeria. A multistage sampling technique was used to select 114 layer producers. The data collected were analyzed using descriptive statistics, Total Factor Productivity, and Tobit regression model. The result of this study showed that though most of the layer producers had access to credit (87.6%), not all of them obtained credit (67.0%) from the sources where credit can be accessed. It also revealed that layer farmers with access to credit (4.88±1.99) were marginally more productive than layer producers without access to credit (4.22±2.22). The study finally revealed that while socio-economic variables such as sex of layer farmers, marital status of layer farmers, household size of layer farmers, and membership of cooperative association significantly influenced productivity of layer farmers, access to credit also significantly influenced the productivity of layer farmers. The study recommended that the government should enforce policies relating to ease of accessing credit from financial institutions.

Antibiotics in eggs: An analytical approach based on low- and high-resolution mass spectrometry techniques

Residues of antibiotics applied in chicken husbandry can be found in eggs for human consumption. To prevent this issue and avoid adverse effects on consumers' health, such as allergies and antimicrobial resistance (AMR), maximum residue limits (LMR) have been established by the European Commission. The aim of this study was to develop a method to extract, detect and quantify antibiotics in eggs, using UHPLC-TOF-MS for semi-quantitative screening and UHPLC-QTRAP-MS/MS for quantitative confirmation, following the performance criteria established by Commission Regulation (EU) Nº 2021/808 for analytical method validation. Different extraction methods were analyzed, with liquid-liquid extraction (LLE) based on acetonitrile (ACN) and ethylenediaminetetraacetic acid (EDTA), proving to be the most efficient method for extraction of antibiotic residues from eggs. UHPLC-TOF-MS and UHPLC-QTRAP-MS/MS were validated for 46 compounds, including penicillin, cephalosporins, tetracyclines, quinolones, macrolides, sulfonamides, and other antibiotics. After validation, robustness of both methods was evaluated by analyzing ten egg samples from backyard production and supermarkets. Erythromycin residues were detected in two eggs from backyard production; residues of up to seven antibiotics were detected in two samples of supermarket eggs. Nonetheless, all positive samples were below the established MRL. The developed methods will be a useful tool to monitor possible contamination in eggs, as they allow the simultaneous detection of several molecules. Furthermore, this methodology can be optimized to reduce the relative matrix effect, extended to a larger group of molecules, include other types of drugs, and be validated for other matrices of animal origin.

Solar Powered Thermoelectric Air Conditioning for Temperature Control in Poultry Incubators

Proper air conditioning is crucial for hatching, growing, and reproducing poultry chickens. The existing methods are often costly and only viable for some chicken farmers. This paper presents a novel solar-powered thermoelectric module that utilizes the Peltier effect for efficient cooling and heating in poultry incubators. The proposed system consists of a Peltier module with cool and hot junctions, powered by a solar panel through a charge controller and battery. The cool junction is located in the chicken-breeding and reproduction unit, while the hot junction is situated in the egg-incubation unit. Temperature controllers maintain the required temperatures of 35–40 °C for the egg-hatching and 10–24 °C for the chicken-growing units. The experimental results demonstrate the system’s effectiveness in maintaining the desired temperatures. This solar-powered thermoelectric air conditioning system offers advantages over traditional methods, including lower energy consumption, reduced costs, and eco-friendliness. It has the potential to benefit off-grid poultry farmers and reduce energy bills for existing chicken farms. The mathematical modeling, load calculations, and prototype results show that the proposed system is best suited for providing the required cooling and heating effects in poultry incubators. This research represents a significant step forward in temperature control for poultry incubators and could revolutionize poultry farming practices, especially in remote locations with limited electricity access.

LEVEL OF BIOSECURITY IMPLEMENTATION IN CLOSED HOUSE BROILER CHICKEN FARMS IN SUSUT DISTRICT, BANGLI REGENCY, BALI

Biosecurity is a management step that must be taken by farmers to prevent disease germs from entering the farm and to prevent diseases on the farm from spreading to other farms. This research aims to determine the implementation and level of application of biosecurity components in closed house cages for broiler chicken breeders in Susut District, Bangli Regency, Bali. The method used is a census of respondents, namely all breeders. The data obtained from the interviews is tabulated and then the percentage of the amount of data that is in accordance with biosecurity implementation practices is calculated. The results show that overall farmers have implemented biosecurity components with each biosecurity component, namely the sanitation component showing the Very Good category (85%), the traffic control component showing the Poor category (38.81%) and the sanitation component showing the Very Good category. (97.7%). From the results obtained, chicken farmers should further improve their biosecurity implementation system, especially for guests/farm workers, because a very big factor in the spread of disease is the traffic component, including people, equipment and vehicles.

The Agricultural Economy of the Sanxingdui Culture (3700–3100 BP): Archaeological and Historical Evidence from the Chengdu Plain

Agriculture is a critical foundation for the development of large-scale complex and urban societies. Sanxingdui, located on the Chengdu Plain in western Sichuan and linked with the ancient Chinese Shu Kingdom, is one of the most distinctive archaeological sites in the world. However, despite its importance, the agricultural economy of the Sanxingdui culture and the Chengdu Plain remains poorly understood and heavily debated. This study synthesizes recent archaeological and historical evidence concerning the agricultural economy underpinning the increasing scale and social complexity at Sanxingdui and the greater Chengdu Plain prior to 2200 BP. Our analysis finds a mixed rice and dry-land millet farming economy, in conjunction with pig and chicken husbandry, during the Sanxingdui culture (3700–3100 BP). This integrated agricultural system likely began with the Baodun culture (4500–3700 BP), passing to the Sanxingdui culture (3700–3100 BP), Shierqiao culture (3100–2600 BP) and later, the Shu culture (2600–2300 BP). In addition, although gathering, hunting, and fishing accounted for relatively low proportions of the overall subsistence, the ancient peoples at Sanxingdui and across the Chengdu Plain continued these practices, supporting a diverse agricultural and food system. Understanding the subsistence at Sanxingdui offers key insights into the development of complex societies in southwest China, the contributions to Chinese culture, and the role of agriculture worldwide.

A multicriteria decision analysis for selecting rainwater harvesting systems in rural areas: a tool for developing countries

Global water provision challenges have promoted decentralized water supply alternatives such as rainwater harvesting systems (RWHS). RWHS sustainability demands involve social, technical, and economic criteria in planning. Generally, in rural areas, water provision is more complex due to multiple uses of water, scattering of households, and low economies of scale. This research proposes a multicriteria tool for selecting RWHS in rural areas, considering social, technical, and economic criteria. The tool was developed by systematically identifying subcriteria and their hierarchization through the analytical hierarchy process (AHP), the technique for order of preference by similarity to ideal solution (TOPSIS), and a case study validation. Seven subcriteria were identified. The hierarchy of criteria was social (49.7%), technical (26.4%), and economic (23.9%). The tool involved: (i) users’ consultation about the perceived ease of use and availability of water sources other than rainwater; (ii) system dimensioning to establish supply size, maintenance requirements, and required water quality; and (iii) costs and benefits estimation. Tool validation in a rural area included the evaluation of the alternatives proposed: (a) alternative 1: potable domestic uses (PD) and non-potable (NPD); (b) alternative 2: PD and NPD, irrigation of crops and chicken farming for self-consumption; and alternative 3: PD and NPD and chicken farming for profit sale. The sensitivity analysis showed the tool’s consistency and robustness. Tool validation highlights the importance of integrating the three dimensions in selecting RWHS. The study provides a systematic methodology to assess and prioritize RWHS, appealing to policymakers, engineers, and practitioners facilitating water management and supply processes in rural areas.

Assessment of biosecurity status of commercial chicken farms from Maharashtra, India

Assessment of biosecurity status of commercial chicken farms from Maharashtra, India

Characterization of the prevalence of Salmonella in different retail chicken supply modes using genome-wide and machine-learning analyses

Salmonella is a foodborne pathogen that causes salmonellosis, of which retail chicken meat is a major source. However, the prevalence of Salmonella in different retail chicken supply modes and the threat posed to consumers remains unclear. The prevalence, serotype distribution, antibiotic resistance, and genomic characteristics of Salmonella in three supply modes of retail chicken (live poultry, frozen, and chilled) were investigated using whole-genome sequencing (WGS) and machine learning (ML). In this study, 480 retail chicken samples from live poultry, frozen, and chilled supply modes in Guangzhou from 2020 to 2021, as well as 253 Salmonella isolates (total isolation rate = 53.1 %), were collected. The prevalence of isolates in the live poultry mode (67.5 %, 81/120) was statistically higher than in the frozen (50.0 %, 120/240) and chilled (43.3 %, 52/120) (P < 0.05) modes. Serotype identification showed significant differences in the serotype distribution of Salmonella in different supply modes. S. Enteritis (46.7 %) and S. Indiana (14.2 %) were predominant in the frozen mode. S. Agona (23.5 %) and S. Saintpaul (13.6 %) were predominant in live poultry, while S. Enteritis (40.4 %) and S. Kentucky (17.3 %) were predominant in chilled mode. Antibiotic testing showed that frozen mode isolates were more resistant; the multidrug-resistant (MDR) rate of isolates in the frozen mode reached 91.8 %, significantly higher than in the chilled (86.5 %) and live (74.1 %) (P < 0.05) modes. WGS was performed on 155 top serotypes (S. Enteritidis, S. Kentucky, S. Indiana, and S. Agona). The antibiotic resistance gene analysis showed that the abundance and carrying rate of antibiotic resistance genes of Salmonella in the frozen mode (54 types, 16.1 %) were significantly higher than in other modes (live poultry: 36 types, 9.4 %, P < 0.05; chilled: 31 types, 11.6 %). The blaNDM-1 and blaNDM-9 genes encoding carbapenem resistance were found in frozen mode isolates on a complex transposon consisting of TnAS3-IS26. Virulence factors and plasmid replicons were abundant in the studied frozen mode isolates. In addition, single nucleotide polymorphism (SNP) phylogenetic tree results showed that in the frozen supply mode, the S. Enteritidis clonal clade continued to contaminate retail chicken meat and was homologous to S. Enteritidis strains found in farm chicken embryos, slaughterhouse chicken carcasses, and patients from hospitals in China (SNP 0 = 10). Notably, the pan-genome-based ML model showed that characteristic genes in frozen and live poultry isolates differed. The narZ gene was a key characteristic gene in frozen isolates, encoding nitrate reductase, relating to anaerobic bacterial growth. The ydgJ gene is a key characteristic gene in the live mode and encodes an oxidoreductase related to oxidative function in bacteria. The high prevalence of live poultry mode Salmonella and the transmission of frozen mode MDR Salmonella in this study pose serious risks to food safety and public health, emphasizing the importance of improving disinfection and cold storage measures to reduce Salmonella contamination and transmission. In conclusion, the continued surveillance of Salmonella across different supply models and the development of an epidemiological surveillance system based on WGS is necessary.

In vitro analysis of quercetin-like compounds from mistletoe Dendrophthoe pentandra (L.) Miq as a potential antiviral agent for Newcastle disease

Background Recent evidence suggests that some flavonoid compounds obtained from crude methanol extract of mistletoe leaves (Dendrophthoe pentandra L. Miq), also known as Benalu Duku (BD), have antimicrobial effects. Thus, the plant has the potential to eliminate viruses that may cause outbreaks in chicken farms. This study aimed to prove the in vitro ability of flavonoid compounds, namely quercetin-like compounds (QLCs), to eliminate field viruses, specifically the Newcastle disease virus (NDV). Methods This research was performed in two stages. An in vitro test was used with a post-test of the control groups designed at a significance of 0.05. BD leaves (5 kg) were extracted using a maceration method with methanol and then separated into hexane, chloroform, ethyl acetate, and methanol fractions. The final extracted products were separated using semi-preparative high-performance liquid chromatography (HPLC) to obtain QLCs. The QLCs were identified and compared with quercetin using HPLC, proton and carbon nuclear magnetic resonance spectrometry, Fourier transform infrared spectrophotometry and ultra-performance liquid chromatography-mass spectrometry. The activity of QLCs was tested in vitro against the NDV at a virulence titter of 10−5 Tissue Culture Infectious Dose 50% (TCID50) in chicken kidney cell culture. Results Solutions of 0.05% (w/v) QLCs were discovered to have antiviral activity against NDVs, with an average cytopathogenic effect antigenicity at a 10−5 dilution (p&lt;0.05). Conclusions QLCs from flavonoids from the leaves of BD have in vitro antiviral bioactivity against NDV at a virulence titter of 10-5 Tissue Culture Infectious Dose 50% (TCID50) in chicken kidney cell culture. QLCs may have the potential to be developed as medicinal compounds for the treatment of other human or animal viral infections.

The Influence of Capital, Food Costs, and Use of Labor on Production and Income of Broiler Chicken Farmers in the Tabanan District, Bali

This research is motivated by the development of broiler chicken farming businesses in the Bali province. This is influenced by the level of demand for the production of meat, eggs and broiler chicken seeds which is increasing day by day, especially in the domestic market. So that it becomes a driving factor for broiler chicken farming businesses to increase their production. The aim of this research is to analyze how capital development, feed costs, and labor use affect the production and income of broiler chicken farmers.This research uses quantitative and qualitative methods. Data was collected using questionnaires and interviews. The respondents in this study were 100 people who met the criteria as business owners and those who produce broiler chicken products. Three hypotheses were formulated and tested using Path Analysis. Meanwhile, qualitative analysis is taken from data interpretation by providing information and explanations. It can be concluded that this research aims to determine the direct influence of capital, feed costs and labor on the production of broiler chicken farmers in Tabanan Regency. To find out the direct influence of capital, feed costs, labor and production on the income of broiler chicken farmers in Tabanan Regency. And to find out the indirect influence of capital, feed costs and labor on income through broiler chicken production in Tabanan Regency.