Chewing Cycles Research Articles

Functional treatment of bilateral posterior crossbite improves mandibular kinematics during chewing.

To evaluate the effects of treatment of bilateral posterior crossbite (BPXB) on mandibular kinematics by the percentage of reverse chewing cycles (RCCs) during soft and hard bolus chewing before and after the correction of the malocclusion with function-generating bite (FGB). This prospective study included 71 subjects: 19 patients with occlusally symmetric BPXB (M = 9; F = 10; mean age 9.3 ± 2.2[yr.mo]), 32 patients with occlusally asymmetric BPXB (19 with more teeth in crossbite on the right side (right prevalent side), M = 7; F = 12; mean age 8.2 ± 1.6 [yr.mo] and 13 on the left side, M = 7; F = 6; mean age 9.6 ± 1.9 [yr.mo]) and 20 controls without malocclusion (M = 8; F = 12; mean age 10.2 ± 1.7 [yr.mo]). Masticatory patterns were recorded before (T0) and after (T1) the correction of the malocclusion with FGB, with the K7-I® kinesiograph using standardized soft and hard boluses. BPXB was corrected in all included patients. At T0, the percentage of RCCs in BPXB was significantly increased compared to controls (P < .0001); symmetric BPXB showed no difference in RCCs between the sides, whereas asymmetric BPXB showed significantly more RCCs on the side with more teeth in crossbite (prevalent side). After treatment with FGB (T1), the percentage of RCCs was significantly reduced in both symmetric BPXB patients (soft bolus, P = .003; hard bolus, P < .001) and asymmetric BPXB patients (prevalent side: soft and hard bolus, P < .00001; non-prevalent side: soft bolus, P = .01 and hard bolus, P = .0002). Functional correction of BPXB with FGB significantly improved mandibular kinematics during chewing.

Reliability between the two-colour chewing gum and the gummy-jelly tests used for the assessment of masticatory performance.

This study aimed to evaluate the reliability of two methods used to assess masticatory performance and attempt to correlate them to achieve interchangeability between the methods. Twelve healthy dentate volunteers (men = 6, women = 6; mean age = 28.3 ± 4.1) with no known dental or medical pathologies were requested to participate in this study. Each participant completed three masticatory performance assessments, including two two-colour mixing-ability tests using chewing-gums(CG: gum#1 and gum#2) and the gummy-jelly (GJ) test. For each method, participants created five samples each (total = 15 measurements per participant, gum#1 = 5, gum#2 = 5, GJ = 5). For the gum#1 and gum#2 methods, the predetermined chewing cycles were fixed at 10, 15, 20, 25 and 30 cycles, and for the GJ method, the time duration was fixed at 10, 15, 20, 25 and 30 s. The parameter measures were submitted to Z-score transformation, and Bland-Altman plots were generated to graphically compare the differences between two techniques against their means. Additionally, mountain plot was used to assess the cumulative distribution of measurement error between the methods. A total of 180 measurements were recorded. There were significant correlations between the number of chewing cycles/chewing time and masticatory performance using the gum#1 (r = -.753; p < .001), gum#2 (r = -.838; p < .001) and GJ (r = .730). When all tests were considered together for each method, significant correlations were found (p < .001). A descriptive range of mean values aiming to produce reference value ranges for predictive purposes was achieved considering the interchangeably among the methods [CG = GJ (VoH-mg = dL): 10 cycle = 10 s: 0.329 = 110; 15 cycles = 15 s: 0.177 = 164; 20 cycles = 20 s: 0.130 = 205; 25 cycles = 25 s: 0.086 = 200; 30 cycles = 30 s: 0.077 = 267]. The strong correlations and high consistency between the two masticatory performance methods found in this study conclude that the two assessment methods are reliable and interchangeable. Further evaluations are warranted to arrive at a conversion formula for translation of the results between the two methods.

The Kinematics of Proal Chewing in Rats.

Chewing kinematics are well-documented in several mammal species with fused mandibular symphyses, but relatively understudied in mammals with an unfused symphysis, despite the fact that more than half of extant Mammalia have an unfused mandibular symphysis. The Wistar brown rat (Rattus norvegicus) is widely used in human health research, including studies of mastication or neurological studies where mastication is the output behavior. These animals are known to have unfused mandibular symphyses and proal jaw (rostrocaudal) motion during occlusion, but the lack of high resolution, 3-dimensional analysis of rat chewing leaves the functional significance of symphyseal mobility unknown. We used biplanar fluoroscopy and the X-ray reconstruction of moving morphology workflow to quantify chewing kinematics in 3 brown rats, quantifying overall jaw kinematics, including motions about the temporomandibular joint and unfused mandibular symphysis. During occlusion, the teeth and the mandibular condyle translate almost exclusively anteriorly (proal) during occlusion, with little motion in any other degrees of freedom. At the symphysis, we observed minimal flexion throughout the chew cycle. Overall, there are fundamental differences in jaw kinematics between rats and other mammals and therefore rats are not an appropriate proxy for ancestral mammal jaw mechanics. Additionally, differences between humans and rat chewing kinematics must be considered when using rats as a clinical model for pathological feeding research.

Influence of the radius of Monson’s sphere and excursive occlusal contacts on masticatory function of dentate subjects

ObjectiveTo determine the influence of the radius of Monson’s sphere, the number of posterior laterotrusive, mediotrusive, and protrusive contacts, and the chewing rate on food comminution. DesignSixty healthy dentate subjects, aged 21.22 ± 2.30 years, were selected. The three-dimensional coordinates of the cusp tips of the lower canine, premolar, and molar teeth were identified from the subjects’ digital models. Monson’s sphere was designed using the simplex method for function minimisation by adjusting the coordinates on its surface. The contacts were verified using 12 µm metal strips in jaw excursions at 0.5, 1.0, 2.0, and 3.0 mm. The masticatory performance and efficiency, swallowing threshold, and chewing rate were assessed through particle size fractionation. Data were analysed with multiple linear regression (α = 0.05). ResultsThe sphere’s radius, laterotrusive and protrusive contacts at 0.5 mm, and chewing rate were found to be negative predictor variables for masticatory performance until 20 chewing cycles (R2 = 0.429). For 40 cycles, the radius and total contacts (0.5 mm) were also explanatory factors (R2 = 0.223). Only the radius (R2 = 0.176) and the chewing rate (R2 = 0.082) were found to be significant for 60 cycles and swallowing threshold, respectively. Masticatory efficiency was influenced by masticatory performance until 40 and 60 cycles, as well as the radius and total contacts at 2.0 and 3.0 mm (R2 = 0.958). ConclusionA larger radius of Monson’s sphere and a greater number of posterior excursive contacts were found to be related to better masticatory function.

Masticatory Function, Sex, and Risk of Dementia Among Older Adults: A Population-Based Cohort Study.

A decline in masticatory function may indicate brain dysfunction related to dementia, but the relationship between masticatory function and dementia risk remains unclear. This study aimed to investigate whether masticatory function is associated with the risk of cognitive decline and dementia. Data were obtained from the nationwide prospective cohort study of randomly sampled community-dwelling Koreans aged ≥ 60 years. The 5,064 non-demented participants, whose number of chewing cycles per bite was assessed by clinical interview, were followed for 8 years with biennial assessments of cognitive performance and clinical diagnoses of all-cause dementia and Alzheimer's disease (AD). Structural brain magnetic resonance imaging was collected from a subset of cohort participants and their spouses for imaging analyses. Males who chewed ≥ 30 cycles/bite had faster decline in global cognition and memory function and were at higher risk for incident all-cause dementia (hazard ratio [HR], 2.91; 95% confidence interval [CI], 1.18-7.18) and AD (HR, 3.22; 95% CI, 1.14-9.11) compared to males with less than 10 cycles/bite. Additionally, increased chewing cycles in males were associated with reduced brain volume, particularly in regions involved in compensatory cognitive control of mastication. There was no significant association between chewing cycles and the risk of dementia or brain volume in females. Older men who frequently chew their meals could be considered a notable population at risk for dementia who should be carefully assessed for their cognitive trajectories.

Impact of Lallemantia royleana seed mucilage on the physicochemical, textural, rheological, and in-mouth breakdown behavior properties of cold-set whey protein isolate emulsion-filled gel

This work focused on the Lallemantia royleana seed mucilage (LRSM) effect on the rheological, physicochemical, and microstructural attributes, and the in vivo oral breakdown of cold-set emulsion-filled gel (EFG) based on the whey protein isolate (WPI). Emulsions containing 0.1 and 0.5% LRSM displayed the largest and smallest droplet sizes (3.38 vs. 1.26 μm) and polydispersity index (0.60 vs. 0.23), respectively. Also, the 0.1–0.5% LRSM-WPI EFGs compared to the control had greater elastic modulus in the linear viscoelastic (LVE) region (7006.1–7167.8 vs. 4438.3 Pa), flow-point stress (484.6–540.1 vs. 327.8 Pa), network strength (7227.8–7451.2 vs. 5380.1 Pa.s1z), network extension (10.91–11.44 vs. 10.05), lower LVE limit (1.74–2.26 vs. 2.53 %), same loss tangent (0.17), similar frequency dependence of elastic modulus (0.087–0.091 vs. 0.095), and inhomogeneous microstructures. Hardness, springiness, and chewiness at 0.1% and adhesiveness and cohesiveness at 0.5% LRSM-WPI EFGs were the highest. Raising the LRSM from 0% to 0.5% improved the water-holding capacity (75.49%–87.68%), swelling ratio (0.20%–2.59%), and decreased syneresis (11.08%–9.13%). The 0.5% LRSM-WPI EFG compared to the control needed a higher chew duration (16.22 vs. 11.80 s) and chew cycle (24.76 vs. 18.31), and its bolus had a smaller particle size (0.94 vs. 1.65 mm).

Influence of Post Length, Post Material, and Substance Loss on the Fracture Resistance of Endodontically Treated Teeth: A Laboratory Study

To evaluate the effects of post length, post material, and substance loss on the fracture resistance of endodontically treated mandibular premolars. A total of 96 extracted human mandibular first premolars were endodontically treated and divided into 12 test groups (n = 8 each) based on the number of residual walls (one/two), post material (glass-fiber/titanium), and post length (5 mm, 7.5 mm, and 10 mm). After luting the posts, specimens received a composite resin core and a crown preparation with a 1.5-mm ferrule. Cast cobalt-chromium crowns were cemented using glass-ionomer cement. After 1,200,000 chewing cycles with a load of 49 N and simultaneous thermocycling (5°C to 55°C), specimens were quasi-statically loaded at 30 degrees to the longitudinal axis of the tooth until fracture. Fracture loads were analyzed using three-way, two-way, and one-way ANOVA (α = .05). Fracture modes were examined under a stereomicroscope (×25 magnification) and recorded. The mean ± SD fracture loads ranged from 642 ± 190 N (one wall, glass fiber, 5 mm) to 1,170 ± 130 N (two walls, titanium, 7.5 mm). The mean fracture load of titanium posts was significantly higher than that of glass-fiber posts (P < .001), and the 7.5-mm post length exhibited significantly higher fracture loads than groups with 5-mm and 10-mm post length (P = .008). Teeth restored with titanium posts revealed considerably higher fracture resistance than teeth restored with glass-fiber posts, especially if 7.5-mm-length posts were used.

A novel image processing procedure for the quantitative evaluation of dental enamel prism arrangement.

Enamel prism is the main microstructural unit of mammalian enamel which composed of hundreds of bioapatite nanocrystals. Prism structure plays a key role in the excellent mechanical performance of dental enamel during millions of chewing cycles without significant remodeling. Thus, quantitative understanding of prism architecture is of utmost importance for biomechanical materials design. To characterize enamel prism orientation quantitatively, a novel image processing method has been developed. Our method is based on scanning electron microscopy images of etched enamel surface and consists of an ellipse fitting procedure, which provides a numerical approximation of prism shape and orientation in the studied cross section. The obtained analytical data allow to construct color coded orientation maps, which provide quick and useful insight into the microstructure of enamel. Besides striking visualization, orientation maps allow to extract and plot the rich information on the azimuthal and inclination angles of the prisms as function of location. Numerical data on prism arrangement can be analyzed using statistical tools over large areas, which paves the way towards quantifying comparative investigation of prism arrangement either in dentistry research or evolution biology. The application of the method is demonstrated for a distal-mesial cross-section of sound human tooth enamel. HIGHLIGHTS: Scanning electron microscopy images of etched enamel surface are analyzed using ellipse fitting. Geometrical parameters of the fitted ellipses provide numerical data of thousands of prisms. Prism arrangement is visualized on color coded orientation maps and analyzed using statistical tools.

Influence of different surface finishing procedures of strength-gradient multilayered zirconia crowns on two-body wear and fracture load: Lithium silicate or leucite glazing versus polishing?

ObjectivesTo examine the influence of different finishing procedures on the surface roughness, wear resistance and fracture load of strength-gradient multilayered zirconia. Materials and methodsZirconia crowns (Multilayer 3D pro; n = 96) were manufactured and treated with a lithium-silicate- (LISI_S), leucite-based glaze spray (LEU_S), leucite-based glaze mass (LEU_M) or polished (POL). Natural molars (CG; n = 24) acted as a control. Roughness was determined on flat glazed specimens (n = 28). Two-body wear was examined by digitalizing and matching the occlusal surface before and after thermo-mechanical aging (6,000 thermal and 1,200,000 chewing cycles). The groups were split to measure fracture load initially and after aging. Kolmogorov-Smirnov, Spearman correlation, Kruskal-Wallis-H, Levene's test, one-way ANOVA with Scheffé post-hoc and Weibull modulus were computed. ResultsPOL presented the lowest and LEU_S the highest roughness. Following POL, no ceramic loss was observed. LISI_S, LEU_S and LEU_M showed lower ceramic wear than the CG. The lowest wear of the antagonist was observed for the CG. LISI_S showed a lower antagonistic wear than LEU_S, LEU_M and POL. LISI_S, LEU_S, LEU_M and POL showed higher fracture load values and Weibull moduli than the CG. Artificial aging did not impact the fracture load or Weibull moduli. SignificanceWith glazed groups showing height losses closer to the CG, glazing should be preferred to polishing when approximating the wear behavior of a natural dentition. Finishing of monolithic zirconia by glazing with a lithium silicate-based spray is recommended to preserve the antagonists. All examined zirconia crowns presented sufficient mechanical properties to withstand masticatory forces, even after prolonged aging.

Bonding Strength of Various Luting Agents between Zirconium Dioxide Crowns and Titanium Bonding Bases after Long-Term Artificial Chewing

The use of hybrid abutment crowns bonded extraorally to a titanium bonding base has aesthetic and biological benefits for the prosthetic rehabilitation of oral implants. The objective of this study was to evaluate the effects of luting agents between a zirconium dioxide crown and the titanium bonding base on crown/abutment retention and the subsequent durability of the prosthetic superstructure. Fifty-six implant abutment samples, all restored with a lower first premolar zirconium dioxide crown, were used and divided into seven groups (n = 8/group) according to the type of luting agent used: group 1, SpeedCEM Plus; group 2, Panavia SA Cement Universal; group 3, Panavia V5; group 4, RelyX Unicem 2 Automix; group 5, VITA ADIVA IA-Cem; group 6, Ketac CEM; and group 7, Hoffmann’s Phosphate Cement. All specimens were subjected to thermomechanical loading (load of 49 N, 5 million chewing cycles and 54.825 thermocycles in water with temperatures of 5 °C and 55 °C). The surviving samples were exposed to a pull-off force until crown debonding from the bonding base. Overall, 55 samples survived the thermomechanical load. Group 2 showed the highest mean pull-off force value (762 N), whereas group 6 showed the lowest mean value (55 N). The differences between the seven groups were statistically significant (ANOVA, p &lt; 0.001). The debonding failure pattern was mainly adhesive and was noticed predominantly at the zirconium dioxide–luting agent interface. Within the scope of the present investigation, it was shown that most of the luting agents are suitable for “cementation” of a zirconium dioxide crown onto a titanium base since the debonding forces are above a recommended value (159 N).

Tongue, palatal, hyoid and pharyngeal muscle activity during chewing, swallowing, and respiration

ObjectiveChewing, swallowing, and respiration are synchronized oropharyngeal functions. This study aimed to analyze the dynamics and coordination during natural chewing and swallowing in relation to respiratory phases. DesignEight oropharyngeal muscles in minipigs were recorded using electromyography, X-ray fluoroscopy, and nasopharyngeal dynamics. Chewing cycles and swallowing episodes were analyzed for timing and activity amplitude along respiratory cycles. Digastric and middle pharyngeal constrictor were used as zero-points for timing analysis in chewing cycles and swallowing episodes, respectively. The beginning of these cycles and episodes were used as the zero-point for timing analysis in respiration during feeding. ResultsThe timing of jaw closing (57.8%) was longer than opening (42.2%) during chewing. Muscle activity occurred 20% later than digastric onsets and 15% earlier than jaw closing phase. Duration of muscle activity was shorter in ipsilateral than contralateral sides except for palatal muscles. Pharyngeal, palatal, and hyoid muscles showed longer durations than tongue muscles in jaw opening (p < 0.05). Palatal and hyoid muscles showed 2-phased activity in chewing while hyoid muscles showed higher amplitude in chewing and swallowing than other muscles. About 80% of the chewing cycles and swallowing episodes occurred in expiration. Nasopharyngeal airflow velocity increased from jaw opening to swallowing while airflow pressure decreased. ConclusionThese findings indicate key activity of palatal and pharyngeal muscles mostly in chewing. The respiratory cycle changes in chewing and swallowing simultaneously with the activation of the tongue, palatal, and pharyngeal muscles. These findings will be useful for further understanding the mechanisms in swallowing and breathing disorders.

Rhythmic chew cycles with distinct fast and slow phases are ancestral to gnathostomes.

Intra-oral food processing, including chewing, is important for safe swallowing and efficient nutrient assimilation across tetrapods. Gape cycles in tetrapod chewing consist of four phases (fast open and -close, and slow open and -close), with processing mainly occurring during slow close. Basal aquatic-feeding vertebrates also process food intraorally, but whether their chew cycles are partitioned into distinct phases, and how rhythmic their chewing is, remains unknown. Here, we show that chew cycles from sharks to salamanders are as rhythmic as those of mammals, and consist of at least three, and often four phases, with phase distinction occasionally lacking during jaw opening. In fishes and aquatic-feeding salamanders, fast open has the most variable duration, more closely resembling mammals than basal amniotes (lepidosaurs). Across ontogenetically or behaviourally mediated terrestrialization, salamanders show a distinct pattern of the second closing phase (near-contact) being faster than the first, with no clear pattern in partitioning of variability across phases. Our results suggest that distinct fast and slow chew cycle phases are ancestral for jawed vertebrates, followed by a complicated evolutionary history of cycle phase durations and jaw velocities across fishes, basal tetrapods and mammals. These results raise new questions about the mechanical and sensorimotor underpinnings of vertebrate food processing. This article is part of the theme issue 'Food processing and nutritional assimilation in animals'.

Dynamic finite element modelling of the macaque mandible during a complete mastication gape cycle.

Three-dimensional finite element models (FEMs) are powerful tools for studying the mechanical behaviour of the feeding system. Using validated, static FEMs we have previously shown that in rhesus macaques the largest food-related differences in strain magnitudes during unilateral postcanine chewing extend from the lingual symphysis to the endocondylar ridge of the balancing-side ramus. However, static FEMs only model a single time point during the gape cycle and probably do not fully capture the mechanical behaviour of the jaw during mastication. Bone strain patterns and moments applied to the mandible are known to vary during the gape cycle owing to variation in the activation peaks of the jaw-elevator muscles, suggesting that dynamic models are superior to static ones in studying feeding biomechanics. To test this hypothesis, we built dynamic FEMs of a complete gape cycle using muscle force data from in vivo experiments to elucidate the impact of relative timing of muscle force on mandible biomechanics. Results show that loading and strain regimes vary across the chewing cycle in subtly different ways for different foods, something which was not apparent in static FEMs. These results indicate that dynamic three-dimensional FEMs are more informative than static three-dimensional FEMs in capturing the mechanical behaviour of the jaw during feeding by reflecting the asymmetry in jaw-adductor muscle activations during a gape cycle. This article is part of the theme issue 'Food processing and nutritional assimilation in animals'.

Three-dimensional mandibular kinematics of mastication in the marsupial Didelphis virginiana.

Didelphis virginiana (the Virginia opossum) is often used as an extant model for understanding feeding behaviour in Mesozoic mammaliaforms, primarily due to their morphological similarities, including an unfused mandibular symphysis and tribosphenic molars. However, the three-dimensional jaw kinematics of opossum chewing have not yet been fully quantified. We used biplanar videofluoroscopy and the X-Ray Reconstruction of Moving Morphology workflow to quantify mandibular kinematics in four wild-caught opossums feeding on hard (almonds) and soft (cheese cubes) foods. These data were used to test hypotheses regarding the importance of roll versus yaw in chewing by early mammals, and the impact of food material properties (FMPs) on jaw kinematics. The magnitude of roll exceeds that of yaw, but both are necessary for tooth-tooth or tooth-food-tooth contact between complex occlusal surfaces. We confirmed the utility of the four vertical kinematic gape cycle phases identified in tetrapods but we further defined two more in order to capture non-vertical kinematics. Statistical tests support the separation of chew cycle phases into two functional groups: occlusal and non-occlusal phases. The separation of slow close into two (occlusal) phases gives quantitative kinematic support for the long-hypothesized multifunctionality of the tribosphenic molar. This article is part of the theme issue 'Food processing and nutritional assimilation in animals'.

Fracture strength of endocrown maxillary restorations using different preparation designs and materials

PurposeThis study investigated the impact of preparation design and material types on fracture strength in maxillary premolars endocrowns after thermodynamic aging. Materials and methodsEighty two-rooted maxillary premolar crowns underwent endodontic treatment (N = 80, n = 10). The teeth were categorized into ten groups (4-mm deep with no intracanal extension lithium disilicate glass ceramic & multilayer zirconia endocrowns (LE0 & ZE0); 4-mm deep with 4-mm intracanal extension in one canal (LE1 & ZE1); 4-mm deep with 2-mm intracanal extensions in both canals (LE2 & ZE2); flat overlays with no endocore (LO & ZO); glass fiber reinforced post & core and crown (LC & ZC)). After cementation, all specimens were subjected to 1500 thermocycles and 1,200,000 chewing cycles with an axial occlusal load of 49 N. A static loading test was performed at a non-axial 45° loading using a universal testing machine and failure modes (Type I: restoration debonding; Type II: restoration fracture; Type III: restoration/tooth complex fracture above bone level; Type IV: restoration/tooth complex fracture below bone level) were evaluated using a stereoscope. Data were ananalzed using 2-way ANOVA and Tukey's tests (alpha = 0.05). ResultsThe endocrowns manufactured from multilayered zirconia and pressed lithium disilicate glass ceramic exhibited a fracture load ranging between 1334 ± 332 N and 756 ± 150 N, with ZC presenting the highest and LE2 the lowest values. The differences were not statistically significant (p > 0.05). ConclusionAll endocrowns tested in this study performed similar considering the different designs and materials tested. The distribution of fracture modes did not differ significantly depending on the design of the restoration and the type of material used.

Unilateral Hypofunction of the Masseter Leads to Molecular and 3D Morphometric Signs of Atrophy in Ipsilateral Agonist Masticatory Muscles in Adult Mice.

Mice are commonly used to study mandibular dynamics due to their similarity in chewing cycle patterns with humans. Adult mice treated unilaterally with botulinum toxin type A (BoNTA) in the masseter exhibit atrophy of this muscle characterized by an increase in the gene expression of atrophy-related molecular markers, and a reduction in both muscle fiber diameter and muscle mass at 14d. However, the impact of this muscle imbalance on the non-treated masticatory muscles remains unexplored. Here, we hypothesize that the unilateral masseter hypofunction leads to molecular and 3D morphometric signs of atrophy of the masseter and its agonist masticatory muscles in adult mice. Twenty-three 8-week-old male BALB/c mice received a single injection of BoNTA in the right masseter, whereas the left masseter received the same volume of saline solution (control side). Animals were euthanized at 2d, 7d, and 14d, and the masticatory muscles were analyzed for mRNA expression. Five heads were harvested at 14d, fixed, stained with a contrast-enhanced agent, and scanned using X-ray microtomography. The three-dimensional morphometric parameters (the volume and thickness) from muscles in situ were obtained. Atrogin-1/MAFbx, MuRF-1, and Myogenin mRNA gene expression were significantly increased at 2 and 7d for both the masseter and temporalis from the BoNTA side. For medial pterygoid, increased mRNA gene expression was found at 7d for Atrogin-1/MAFbx and at 2d-7d for Myogenin. Both the volume and thickness of the masseter, temporalis, and medial pterygoid muscles from the BoNTA side were significantly reduced at 14d. In contrast, the lateral pterygoid from the BoNTA side showed a significant increase in volume at 14d. Therefore, the unilateral hypofunction of the masseter leads to molecular and morphological signs of atrophy in both the BoNTA-injected muscle and its agonistic non-injected masticatory muscles. The generalized effect on the mouse masticatory apparatus when one of its components is intervened suggests the need for more clinical studies to determine the safety of BoNTA usage in clinical dentistry.

IN VITRO SURVIVAL AND FRACTURE FORCE OF LITHIUM DISILICATE HYBRID ABUTMENT CROWNS.

To evaluate the behavior of hybrid abutment crowns fabricated from monolithic lithium disilicate ceramic (LDC) and to compare the influence of different in vitro artificial aging protocols. A total of 32 monolithic hybrid abutment crowns of monolithic LDC were fabricated. Of these, 24 were artificially aged using three different protocols up to a 20 year simulation (1.2 × 106, 2.4 × 106, 4.8 × 106 chewing cycles, thermocycling), a control group underwent no artificial aging (n &#61; 8, N &#61; 32). Load-to-failure tests were conducted for all specimens, and failure values were compared (P < .05). All specimens passed in vitro aging. Mean failure load values between 532.6 and 562.8 N were found but did neither differ significantly among the test groups nor from the control group. Within the limitations of this in vitro pilot study, hybrid abutment crowns manufactured from monolithic LDC seem to offer appropriate long-lasting mechanical stability over a simulation period up to 20 years. The failure values and complication pattern seem to be independent of several aging protocols in this test set-up.

Side switch frequency while masticating different chewing materials, and its relationship with other masticatory behaviors and sensory perceptions

ObjectiveThis cross-sectional study aimed to establish normative values for masticatory side switch (MSS) frequency in young Mexican adults and to assess the relationship between various indices and MSS frequency when masticating different chewing materials. DesignWe enrolled 101 dentate adults and performed four masticatory assays that involved masticating different chewing materials (i.e., two-colored chewing gum, sweet cracker, salty cracker, and bread). Participants were asked to eat and swallow these foods and to chew the gum for 40 cycles and the following indices were determined: MSS index (MSSI), unilateral chewing index, chewing cycle duration, and number of cycles before terminal swallowing. The participants then rated perceived flavor intensity, salivary flow, and muscle fatigue during each trial. ResultsThe MSSI ranged from 0.03–0.06 (10th percentile) to 0.48–0.54 (90th percentile). A repeated-measures general linear model revealed a mean MSSI value of 0.28 (95 %CI, 0.25–0.30) adjusted by several factors. Male sex, soft food, and the last chewing period were associated with lower MSS frequency. Spearman’s test showed a high correlation for the MSSI among the different foods. MSSI correlated negatively with the unilateral chewing index for each chewing material and with number of cycles for the sweet cracker. However, no significant correlation was detected between MSSI and sensory perception. ConclusionsIn healthy dentate individuals, the mean MSS relative frequency is 25–30 % with an 80-central percentile of 5–50 % of the maximum possible side changes. Lower MSS frequencies were detected in men, when chewing soft food, and during the final chewing period.

DESIGNING OF A CUSTOM DEVICE FOR INVESTIGATING THE MASTICATORY AND TEMPORAL MUSCLES IN PATIENTS EXPERIENCING TMJ DYSFUNCTION

Relevance: At present, electromyography (EMG) serves as the standard method for diagnosing the condition of masticatory muscles. This technique enables the evaluation of bioelectric potentials, including bioelectric rest, average biopotential amplitude, the duration of chewing cycle and the number of chewing movements performed. In dental practice, EMG is conducted using electromyographs with electrodes attached to the skin using adhesive or electrically conductive gel. However, this installation process is often time-consuming, and the mobility of the sensors can compromise result reliability. Furthermore, when performing multiple EMG studies on a patient during different stages of treatment, there is no guarantee that the motor point will be consistently selected, leading to inaccurate data and comparisons. The aim is to develop and implement a personalized device design for performing EMG on masticatory muscles, ensuring consistent repetition of the study in clinical practice. Materials and methods: Through a comprehensive analysis of scientific literature, information sources were utilized to develop and manufacture an EMG device. Results: The technical outcome achieved includes a simplified methodology, improved sensor fixation, reduced EMG duration, and the ability to replicate the study consistently at various stages of a patient's treatment. Conclusion: The developed device and EMG methodology offer a simplified approach to obtaining accurate study results with reduced time requirements. Additionally, if repetition of the study is necessary, the obtained data would be representative.

An Ex-Vivo Model for Investigating Bacterial Extrusion from Infected Root Canals during Masticatory Function

IntroductionThe extrusion of bacteria from infected root canals may lead to increase in symptoms, expansion of periapical lesions, and contribution to systemic diseases. The aim of this study is to investigate a potential proof-of-concept model to study the extent to which bacteria can escape from infected root canals under dynamic loading (simulated chewing). MethodsThe study was completed in 2 experiments performed at 2 institutions. Biofilms of Streptococcus intermedius in the first experiment and S. intermedius and Actinomyces naeslundii were allowed to grow in root canals of single-rooted extracted teeth for 3 weeks. The roots of the teeth were suspended in a small chamber containing dental transport medium and were mounted on a lower sample holder of a chewing simulator. In the experimental group, simulated chewing cycles equivalent to 1 year of function were conducted, and then bacterial migration was quantified and compared with stationary teeth. ResultsAll experimental samples of the loading group revealed bacterial penetration in both experiments. Several of the unloaded samples revealed no bacterial penetration. In the first experiment, a significantly higher number of bacteria were able to escape into the periapex of the loaded group compared with the unloaded group (P = .017). In the second experiment, there was no significant difference between the 2 bacterial species used in the amount of extruded bacteria; however, there was a highly significant effect for occlusal loading (P = .0001). ConclusionsThe potential for occlusal forces to enhance bacterial extrusion from infected root canals should be further explored.