Chenopodium Ambrosioides Research Articles

Antibacterial Activity Test Of Epazote Leaf Infusion (Dysphania ambrosioides L.) Against Escherichia coli Bacteria

The most common health problem in Indonesia is bacterial infection. Dysphania ambrosioides L., also known as epazote leaves, is an herbal plant that has antimicrobial potential. This research was conducted to see at what concentration there is antibacterial activity of epazote leaf infusion Dysphania ambrosioides L. against Escherichia coli bacteria. This laboratory experimental study used a complete randomized design (RAL) research. Antibacterial activity testing of epazote leaves was carried out using the disc paper diffusion method with concentrations of 50%, 75%, and 100%. Epazote leaves are boiled in an infusion pan for 15 minutes, until the temperature reaches 90 degrees Celsius. Ampicillin positive control 10 ?g/disc. Based on the test results of secondary metabolites of positive epazote leaf infusion there are alkaloids, flavonoids, tannins, saponins, triterpenoids, and phenolics. The results of the study showed that infusion of epazote leaves inhibited Escherichia coli bacteria by diameter inhibition. Average at a concentration of 50% = 14.04 mm, 75% = 16.92 mm, 100% = 17.94 mm, which means that there is antibacterial activity of epazote leaf infusion against Escherichia coli bacteria.

Ethnopharmacological Insights into the Use of Chenopodium ambrosioides for Cough Treatment among Togolese Traditional Healers

Togo has an ethnobotanical potential with a great diversity of medicinal plants widely used to treat human diseases. Cough associated with respiratory diseases is one of the pathologies traditionally treated with medicinal plants in Togo. Chenopodium ambrosioides (L.) is a species used in the traditional treatment of cough in Togo. The aim of this study was to document the ethnopharmacological uses of Chenopodium ambrosioides in Togolese traditional medicine for treatment of cough. An ethnopharmacological survey was conducted using the semi-structured interview method among Traditional Medicine Practitioners (TMPs) from six health districts spread across four health regions. The ethnopharmacological and ethnobotanical data collected were analyzed using the User Value (UV) and Frequency of Citation (FC) of medicinal plants associated with the species. A total of 127 TMPs participated in this study and 96 (76%) of them were male and 31 (34%) were female. 73% of the informants claimed usually use Chenopodium ambrosioides in the cough treatment compared to 27% who claimed to not use it to treat cough. Chenopodium ambrosioides was used in combination with fifty-two (52) other botanical species in the treatment of cough and in the study area, Abrus precatorius Linn. (13.59%) followed by Ocimum gratissimum Linn. (11.58%), Zingiber officinale Rosc. Trans. Linn. (9.73%), Mangifera indica Linn. (6.21%), Eucalyptus citriodora Hooker (4.87%), Xylopia aethiopica (Dunal) A. Rich. (4.36%) and Eugenia caryophyllata Thunb. (4.19%) were the most popular plants used for cough treatment among the plants recorded. Sixteen (16) other pathologies including pulmonary infections are also treated by Chenopodium ambrosioides (L.). Abscesses, intestinal parasitic diseases, incurable wounds, stomach aches and headaches are the most pathologies commonly treated by this plant. The whole plant (72%) and leaves (22%) are the primary parts utilized for medicinal purposes. Decoction (77.50%) is the main preparation method, with oral (80.83%) and cutaneous (10.83%) routes being the most common modes of administration. The findings from this study provide a solid ethnopharmacological foundation for future pharmacological and toxicological investigations into Chenopodium ambrosioides (L.).

Phytochemical Composition, Antioxidant and Antimicrobial Activities of some Dysphania ambrosioides Extracts

Phytochemical Composition, Antioxidant and Antimicrobial Activities of some Dysphania ambrosioides Extracts

Ethnobotanical survey of medicinal plants in a Caatinga area, in the municipality of Crato (Ceará, Brazil)

The objective of this study was to carry out an ethnobotanical survey of medicinal species and verify the versatility of these species. The research was carried out in the municipality of Crato, in São Bento neighborhood, from semi-structured interviews seeking information on the use of medicinal plants and their therapeutic indications in the region. Versatility was achieved through relative importance. A total of 21 species were described (most of which are exotic), distributed in 16 families. From the total of species, there were 04 with great versatility regarding the use of Mentha spicata L., Chenopodium ambrosioides L., Ruta graveolens L., Rosmarinus officinalis L. The most representative families were Lamiaceae and Rutaceae. The main parts used of the plants were leaves (42.31%), bark (30.76%), inner bark (7.69%), root, flower, and fruit (3.85% each). The most frequent forms of preparation were infusion (54.55%), lambedor (24.24%), sauce (12.12%), pulp (6.06%) and juice (3.03%). The plants are used for various health problems like flu, fever, headache, and inflammation in general. The data show that local populations hold knowledge that may be useful as a subsidy for ethnopharmacobotanical and phytochemical studies that may lead to the discovery of new herbal medicines.

Ethnobotanical survey of five wild medicinal plants used by local population in Taza province (Northeastern Morocco)

Since ancient times, Moroccan people have used medicinal and aromatic plants as traditional medicine to treat various ailments. However, few studies have been made to document and promote traditional knowledge. The present study aims to collect information about folk knowledge on the medicinal use of five plants, namely Globularia alypum, Inula viscosa, Chenopodium ambrosioides, Ajuga iva, and Urtica dioca in Taza province (northeastern Morocco). We used semi-structured and unstructured interviews with herbalists, traditional healers, and the local population in Taza province. Indeed, 240 questionnaires were collected to gather information about each plant (parts used, methods of preparation and administration, treatable diseases, etc.). The collected data were analyzed using quantitative indices such as the use value (UV), fidelity level (FL), and informant agreement ratio factor (IAR). Most of the interviewees were 60 years old (58.33%), and 70% of them were women. Nearly 60 % of the interviewees were illiterate, and heritage related to traditional medicine, was the main source of knowledge (50%). Ten indications were reported for the studied species in traditional medicine where dermatological and gastrointestinal disorders had the highest use reports, and two species had the highest FL (100%). The highest UV was found for C. ambrosioides (0.44) and A. iva (0.31). The main preparation forms were infusion (40%) and decoction (22%). The most frequently used plant parts were leaves (55.83%). The wide traditional uses of the studied species are largely supported. Future experimental research should be focused on their pharmacological properties and mechanisms of action.

Hallazgos histopatológicos en nematodos Anisakidae, expuestos a extractos acuosos vegetales con capacidad nematicida <i>in vitro</i>

Objetive. Expose nematodes of the Anisakidae family to different aqueous extracts and identify the aqueous extracts with nematicidal capacity. Material and methods. The concentrations of the bioactive compounds of the aqueous extracts of epazote (Dysphania ambrosioides), onion (Allium cepa), siempre viva (Kalanchoe pinnata) and xoconostle (Opuntia oligacantha) were identified. Live parasites of the Anisakidae family were obtained from Lisa fish (Mugilidae), to be exposed to different concentrations of aqueous extracts. Results. K. pinnata and O. oligocantha presented a higher concentration of bioactive components of polyphenols, flavonoids and tannins; as well as in the antioxidant activity of DPPH and ABTS. Parasite mortality occurred at a concentration of 855 mg/mL for K. pinnata of 100% at 48 hours, and in pulp and whole fruit of O. oligacantha, with mortality of 66% at 72 hours. The main histopathological changes caused by K. pinnata were muscle vacuoles; the whole fruit of O. oligocantha degeneration of the intestinal epithelium and vacuolization; the seed caused edema, intestinal degeneration, and vacuolization. Conclusions. The results indicate that the use of aqueous extracts of K. pinnata and O. oligacantha on nematodes of the Anisakidae family are an option for their use as nematicidal agents.

Comprehensive phytochemical and toxicological analysis of Chenopodium ambrosioides (L.) fractions.

Chenopodium ambrosioides aerial parts have been historically employed in traditional medicine for addressing various ailments such as headaches, abdominal discomfort, joint issues, and respiratory disorders, alongside treatments for lice and warts. This study aimed to conduct a comprehensive phytochemical analysis of C. ambrosioides and assess the acute and subacute toxicity of oral treatments using fractions in preclinical trials. Spectrophotometric analysis via LC-MS/MS was used to characterize the plant's chemical composition. Acute toxicity evaluation followed Organisation for Economic Co-operation and Development code 42 guidelines, conducted on adult male and female Wistar strain mice. Subsequently, Swiss mice were divided into six groups for the subacute toxicity study, receiving oral doses of 200 mg/kg extracts and fractions for 28 days. Daily observations and biochemical analyses were performed, with LC-MS/MS revealing a diverse array of compounds including organic acids, flavonoids, phenolic acids, rutin, hesperidin, nicotiflorine, and fumaric acid. Results indicated no lethality or alterations in body weight in treated groups, though some organ weight changes were noted. Biochemical analyses demonstrated values within the normal range for all groups, suggesting that the treatments did not induce adverse effects. Acute and subacute treatments with fractions did not result in lethality or toxic alterations at therapeutic doses, implying the safety of the product at appropriate levels. This study underscores the potential of C. ambrosioides as a safe therapeutic option warranting further exploration.

Potential anti-liver cancer targets and mechanisms of kaempferitrin based on network pharmacology, molecular docking and experimental verification

AimKaempferitrin is an active component in Chenopodium ambrosioides, showing medicinal functions against liver cancer. This study aimed to identify the potential targets and pathways of kaempferitrin against liver cancer using network pharmacology and molecular docking, and verify the essential hub targets and pathway in mice model of SMMC-7721 cells xenografted tumors and SMMC-7721 cells. MethodsKaempferitrin therapeutical targets were obtained by searching SwissTargetPrediction, PharmMapper, STITCH, DrugBank, and TTD databases. Liver cancer specific genes were obtained by searching GeneCards, DrugBank, TTD, OMIM, and DisGeNET databases. PPI network of “kaempferitrin-targets-liver cancer” was constructed to screen the hub targets. GO, KEGG pathway and MCODE clustering analyses were performed to identify possible enrichment of genes with specific biological subjects. Molecular docking and molecular dynamics simulation were employed to determine the docking pose, potential and stability of kaempferitrin with hub targets. The potential anti-liver cancer mechanisms of kaempferitrin, as predicted by network pharmacology analyses, were verified by in vitro and in vivo experiments. Results228 kaempferitrin targets and 2186 liver cancer specific targets were identified, of which 50 targets were overlapped. 8 hub targets were identified through network topology analysis, and only SIRT1 and TP53 had a potent binding activity with kaempferitrin as indicated by molecular docking and molecular dynamics simulation. MCODE clustering analysis revealed the most significant functional module of PPI network including SIRT1 and TP53 was mainly related to cell apoptosis. GO and KEGG enrichment analyses suggested that kaempferitrin exerted therapeutic effects on liver cancer possibly by promoting apoptosis via p21/Bcl-2/Caspase 3 signaling pathway, which were confirmed by in vivo and in vitro experiments, such as HE staining of tumor tissues, CCK-8, qRT-PCR and Western blot. ConclusionThis study provided not only insight into how kaempferitrin could act against liver cancer by identifying hub targets and their associated signaling pathways, but also experimental evidence for the clinical use of kaempferitrin in liver cancer treatment.

Insecticidal activity on Tribolium castaneum and Phenacoccus solenopsis and characterization of active phytonanoparticles

Phytonanoparticles can be used as an alternative method for pest control in agriculture. The objective of this study was to evaluate the insecticidal activity of mono, bi and trimetallic phytonanoparticles (PNPs) synthesized from cachanilla (Pluchea sericea Nutt. Coville) and epazote (Chenopodium ambrosioides L.) against the red flour beetle (Tribolium castaneum) and the cotton mealybug (Phenacoccus solenopsis). Overall, T. castaneum showed greater sensitivity to PNPs than P. solenopsis. The trimetallic PNPs, Zn/Cu/Mn of P. sericea showed significantly higher effect against T. castaneum than the crude aqueous extract of P. sericea. No other PNPs showed more effect than their respective aqueous plant extract. Physicochemical characterization of the most active PNPs showed that the Zn/Cu/Mn NP of P. sericea had a strong zeta potential of +200 mV, hydrodynamic size of 16 nm, and contains zinc (6.73%), copper (0.13%), and manganese (4.69%). This study suggests that the trimetallic Zn/Cu/Mn PNPs of P. sericea have high insecticidal effect on T. castaneum, but low toxicity on P. solenopsis. Additional research on the mode of action of PNPs on insect targets is required.

Anti- Leishmanial activity of a traditional indian herbal plant Chenopodium ambrosioides

The existing antileishmanial drugs for complete cure of visceral leishmaniasis( Kala azar) are limited The available drugs are either toxic or less effective leading to disease relapse or conversion to postkala ?azar dermal leishmaniasis Several herbal extracts have been shown to have antileishmanial activity... In the present study, In vitro anti- leishmanial activitiy of Ethanolic ,Methanolic , Benzene, and Chloroform extracts of Chinopodiumambrosioides have been evaluated . Among those extracts ethanolic extract of Chinopodiumambrosioides shows effective antileishmanial activity against promastigote form of leishmanial donovani. ascoridole present in C.ambrosioides which may be responsible for inhibition the growth of parasites. It was found that IC 50 = 150 ?g /ml( 50% inhibition at 150 ?g /ml) and IC90 =200 ?g /ml (complete inhibition at 200?g /ml).. KEYWORDS :Visceral leishmaniasis, Chinopodium ambrosioides,Leishmaniadonovani,

Diversity and functional traits of seed endophytes of Dysphania ambrosioides from heavy metal contaminated and non-contaminated areas.

Seed endophytes played a crucial role on host plants stress tolerance and heavy metal (HM) accumulation. Dysphania ambrosioides is a hyperaccumulator and showed strong tolerance and extraordinary accumulation capacities of multiple HMs. However, little is known about its seed endophytes response to field HM-contamination, and its role on host plants HM tolerance and accumulation. In this study, the seed endophytic community of D. ambrosioides from HM-contaminated area (H) and non-contaminated area (N) were investigated by both culture-dependent and independent methods. Moreover, Cd tolerance and the plant growth promoting (PGP) traits of dominant endophytes from site H and N were evaluated. The results showed that in both studies, HM-contamination reduced the diversity and richness of endophytic community and changed the most dominant endophyte, but increased resistant species abundance. By functional trait assessments, a great number of dominant endophytes displayed multiple PGP traits and Cd tolerance. Interestingly, soil HM-contamination significantly increased the percentage of Cd tolerance isolates of Agrobacterium and Epicoccum, but significantly decreased the ration of Agrobacterium with the siderophore production ability. However, the other PGP traits of isolates from site H and N showed no significant difference. Therefore, it was suggested that D. ambrosioides might improve its HM tolerance and accumulation through harboring more HM-resistant endophytes rather than PGP endophytes, but to prove this, more work need to be conducted in the future.

Phytoremediation potential and vegetation assessment of plant species growing on multi-metals contaminated coal mining site

This study aims to evaluate the plant species potential to accumulate, concentrate and translocate the heavy metals around the coal mining contaminated site with heavy metals at Harnoi, Abbottabad. The phytosociological surveys involve the systematic study of plant communities within the particular area to show their composition, structure and distribution showed that the contaminated coal mining-associated area was poor in vegetation. Among these, 11 plant species with higher important values (IV) are collected with associated soil and analyzed for the total concentrations of Cadmium (Cd), Copper (Cu), Chromium (Cr), Lead (Pb) and Nickel (Ni) using Atomic Spectrophotometer. The phytoremediation indices (BAF, BCF, TF and TI) were used to evaluate the multi-metals hyperaccumulator and stabilizer plant species. Dodonaea viscosa was evaluated as multi-metals (Cd, Cu and Ni) stabilizer. While the Ajuga bracteosa and Sonchus espera, Sisybrium officinale and Platango ovata stabilize Cd and Cr respectively. The other plant species that can stabilize as single heavy metal are Ajuga bracteosa and Sonchus espera (Cd), Sisybrium officinale and Platango ovata (Cr) and Amaranthus spinosus (Ni) respectively. While the multi-metals accumulator plant species are Bidens pilosa (Cu, Pb and Ni), Chenopodium ambrosioides (Cd, Cu and Ni), Amaranthus spinosus (Cd, Cu and Cr), Ajuga bracteosa (Pb and Ni) and Rumex hastatus (Cd and Ni). However, the single heavy metal accumulator plant species are Sonchus espera (Pb), Conyzea Canadensis (Ni), Platango ovata and Malvastrum coromandelianum (Cu) respectively. These plants could find valuable applications in practical phytoremediation for the remediation near mining tailings at Abbottabad. Moreover, the use of local plants is a promising approach not only for in situ accumulation and stabilization of heavy metals but also for tolerance and environmental adaptations in the contaminated area.

Complete Chloroplast Genome of Krascheninnikovia ewersmanniana: Comparative and Phylogenetic Analysis.

Krascheninnikovia ewersmanniana is a dominant desert shrub in Xinjiang, China, with high economic and ecological value. However, molecular systematics research on K. ewersmanniana is lacking. To resolve the genetic composition of K. ewersmanniana within Amaranthaceae and its systematic relationship with related genera, we used a second-generation Illumina sequencing system to detect the chloroplast genome of K. ewersmanniana and analyze its assembly, annotation, and phylogenetics. Total length of the chloroplast genome of K. ewersmanniana reached 152,287 bp, with 84 protein-coding genes, 36 tRNAs, and eight rRNAs. Codon usage analysis showed the majority of codons ending with base A/U. Mononucleotide repeats were the most common (85.42%) of the four identified simple sequence repeats. A comparison with chloroplast genomes of six other Amaranthaceae species indicated contraction and expansion of the inverted repeat boundary region in K. ewersmanniana, with some genes (rps19, ndhF, ycf1) differing in length and distribution. Among the seven species, the variation in non-coding regions was greater. Phylogenetic analysis revealed Krascheninnikovia ceratoides, Dysphania ambrosioides, Dysphania pumilio, and Dysphania botrys to have a close monophyletic relationship. By sequencing the K. ewersmanniana chloroplast genome, this research resolves the relatedness among 35 Amaranthaceae species, providing molecular insights for germplasm utilization, and theoretical support for studying evolutionary relationships.

Insecticidal Activity of Essential Oils and Their Synergistic Effect on Improving the Efficacy of β-Cypermethrin against Blattella germanica.

We screened the contact activity of 32 commercial essential oils (EOs) and their synergistic effect with β-cypermethrin against Blattella germanica. Results showed that the most effective EOs against B. germanica were from Illicium verum, Syzygium aromaticum, and Cinnamomum camphora, with LD50 values of less than 500 μg/insect. The most potent synergistic effects of β-cypermethrin on B. germanica were from Dysphania ambrosioides and Mentha canadensis. Both oils have a co-toxic factor of 133.33. The results of the major compound testing of the EOs showed that trans-anisaldehyde and thymol have the best insecticidal activity against B. germanica, with LD50 values of 141.30 and 138.61 μg/insect, respectively. The compounds with the best synergistic effect on β-cypermethrin were γ-terpinene and linalool at a concentration of 0.5%. The co-toxic factors for γ-terpinene and linalool were 150 and 133.33, respectively, which were similar to the synergistic effect observed with 2% piperonyl butoxide.

Improving Lead Phytoremediation Using Endophytic Bacteria Isolated from the Pioneer Plant Ageratina adenophora (Spreng.) from a Mining Area.

This study aimed to isolate and characterise endophytic bacteria from the pioneer plant Ageratina adenophora in a mining area. Seven strains of metal-resistant endophytic bacteria that belong to five genera were isolated from the roots of A. adenophora. These strains exhibited various plant growth-promoting (PGP) capabilities. Sphingomonas sp. ZYG-4, which exhibited the ability to secrete indoleacetic acid (IAA; 53.2 ± 8.3 mg·L-1), solubilize insoluble inorganic phosphates (Phosphate solubilization; 11.2 ± 2.9 mg·L-1), and regulate root ethylene levels (1-aminocyclopropane-1-carboxylic acid deaminase activity; 2.87 ± 0.19 µM α-KB·mg-1·h-1), had the highest PGP potential. Therefore, Sphingomonas sp. ZYG-4 was used in a pot experiment to study its effect on the biomass and Pb uptake of both host (Ageratina adenophora) and non-host (Dysphania ambrosioides) plants. Compared to the uninoculated control, Sphingomonas sp. ZYG-4 inoculation increased the biomass of shoots and roots by 59.4% and 144.4% for A. adenophora and by 56.2% and 57.1% for D. ambrosioides, respectively. In addition, Sphingomonas sp. ZYG-4 inoculation enhanced Pb accumulation in the shoot and root by 268.9% and 1187.3% for A. adenophora, and by 163.1% and 343.8% for D. ambrosioides, respectively, compared to plants without bacterial inoculation. Our research indicates that endophytic bacteria are promising candidates for enhancing plant growth and facilitating microbe-assisted phytoremediation in heavy metal-contaminated soil.

Response of plants and soils to inundation duration and construction of the plant‒soil association mode in the hydro‒fluctuation belt of the reservoir wetland

Hydro-Fluctuation Belt (HFB), a periodically exposed bank area formed by changes in water level fluctuations, is critical for damaging the reservoir wetland landscape and ecological balance. Thus, it is important to explore the mechanism of hydrological conditions on the plant-soil system of the HFB for protection of the reservoir wetland and landscape restoration. Here, we investigated the response of plant community characteristics and soil environment of the HFB of Tonghui River National Wetland Park (China), is a typical reservoir wetland, to the duration of inundation, as well as the correlation between the distribution of dominant plants and soil pH, nutrient contents, and enzyme activity by linear regression and canonical correlation analyses. The results show that as the duration of inundation decreases, the vegetation within the HFB is successional from annual or biennial herbs to perennial herbs and shrubs, with dominant plant species prominent and uneven distribution of species. Soil nutrient contents and enzyme activities of HFB decreased with increasing inundation duration. Dominant species of HFB plant community are related to soil environment, with water content, pH, urease, and available potassium being principle soil environmental factors affecting their distribution. When HFB was inundated for 0–30 days, soil pH was strongly acidic, with available potassium content above 150 mg kg−1 and higher urease activity, distributed with Arundo donax L., Polygonum perfoliatum L., Alternanthera philoxeroides (Mart.) Griseb., and Daucus carota L. communities. When inundated for 30–80 days, soil pH was acidic, with lower available potassium content (50–150 mg kg−1) and urease activity, distributed with Beckmannia syzigachne (Steud.) Fern.+ Polygonum lapathifolium L., Polygonum lapathifolium L., Medicago lupulina L. + Dysphania ambrosioides L. and Leptochloa panicea (Retz.) Ohwi communities. Using the constructed HFB plant-soil correlation model, changes in the wetland soil environment can be quickly judged by the succession of plant dominant species, which provides a simpler method for the monitoring of the soil environment in the reservoir wetland, and is of great significance for the scientific management and reasonable protection of the reservoir-type wetland ecosystem.

USO DE PLANTAS MEDICINAIS DA COMUNIDADE MARRUÁS DO MUNICÍPIO DE CRAÍBAS-AL, COMO FERRAMENTA NA CONSTRUÇÃO DO CONCEITO DE EDUCAÇÃO AMBIENTAL.

Environmental awareness was applied in this work with the aim of instigating environmental awareness in local communities in the Alagoas countryside, with the aim of building, together with the community, a concept of environmental education, using medicinal plants as a tool. For this purpose, visits were made to the target communities, and semi-structured questionnaires were applied, emphasizing the informants' socio-economic data, as well as ethnobotanical data. The study was carried out in the Marruás community, district of Craíbas – AL, between the months of February and October 2010. 100 interviews were carried out with residents, aged 12 to 80, appointed by the community leader. 40 plant species were surveyed, distributed in 35 families. Most interviewees cited Myracrodruon urundeuva Fr. Allem, known as aroeira, as the most used, followed by Melissa officinalis L. (lemon balm) and Cymbopogon citratus (DC) Stapf. (Holy grass). The predominance of leaves (97%) was observed in relation to other vegetative organs, followed by the bark (6%). The local disappearance of some species such as Chenopodium ambrosioides L. (mastruz) and Eucalyptus globulus Labill was recorded. (eucalyptus). The data obtained reveals the need for instructions to promote the sustainable use of medicinal plants in communities like this, creating a perspective of conservation and management of local vegetation.

Medicinal plants, biodiversity, and local communities. A study of a peasant community in Venezuela

ObjectiveThe main objective of this research was to record ethnobotanical data on the use and exploitation of medicinal plants, highlighting their importance for the biodiversity, culture, and tradition of a peasant community in Venezuela.Sample/methodThe study involved a population of 120 individuals, from which a sample size of 34 people was calculated using the formula for finite populations. A simple random sampling technique was employed, and all the participants were administered the TRAMIL (Traditions Medicine in Island) survey.Statistical analysisThe ethnopharmacological table was constructed, and descriptive statistics were used for analysis.ResultsA total of 116 species of medicinal plants were documented to treat various health conditions. The informants reflected through their responses that they used medicinal plants in the first instance to address a health condition, employing varied forms of plant preparation, which include decoction (65.16%), raw consumption (16.77%), maceration (8.38%), and infusion (7.09%). The most commonly used plant parts are leaves, flowers, fruits, bark, peels, roots, and bulbs, while the most commonly used botanical families are Lamiaceae, Fabaceae, Rutaceae, Malvaceae, Verbenaceae, Acanthaceae, Asteraceae, and Euphorbaceae. On the other hand, the species with the highest TRAMIL Significant Use Level were Oregano orejón (Coleus amboinicus Lour.) (68.29), Malojillo [Cymbopogon citratus (D.C.) Stapf.] (60.97), Tua (Jatropha gossypiifolia L.) (34.15), Colombiana [Kalanchoe pinnata (Lam.) Pers.] (34.15), Poleo [Micromeria brownei (Sw.) Benth.] (29.27), Pasote (Chenopodium ambrosioides L.) (29.27), Llantén (Plantago major L.) (26.83), Te negro [Phyla stoechadifolia (L.) Small] (26.83), Yerbabuena (Mentha sp.) (21.85), and Curia (Justicia pectoralis Jacq.) (21.95).ConclusionThe community of El Onoto de El Valle de Tucutunemo, Aragua State, Venezuela has a notable utilization of medicinal plant species in their instance to treat different health conditions, with the predominant focus on treating flu and stomach ailments. It is important to emphasize that all individuals approached through various data collection instruments reported using medicinal plants, both individually and within their families, spanning a wide range of ages from children to the elderly. This reflects that the use of medicinal plants is part of their cultural heritage and ancestral roots.

In vivo protective effects of vitamin C against cyto-genotoxicity induced by Dysphania ambrosioides aqueous extract

Abstract Dysphania ambrosioides (L.) holds a prominent place in Moroccan folk medicine due to its therapeutic attributes. Despite its widespread use, instances of inadvertent intoxication linked to its consumption have been reported. This study aims to evaluate the potential cytogenotoxic effects of D. ambrosioides leaf aqueous extract (DAAE) and explore the prospective protective role of vitamin C (l-ascorbic acid) through the micronucleus test conducted on (1) Vicia faba root-tip meristem and (2) mouse bone marrow cells. In addition, antioxidant enzyme activities, specifically superoxide dismutase (SOD) and catalase (CAT), were evaluated in V. faba treated with DAAE. After a 7-day daily administration of DAAE to mice, serum biochemical parameters (aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, creatinine, and uric acid) were measured, and histological examination of liver and kidney tissues was performed. The results indicated that DAAE had dose-dependent cytotoxic and genotoxic effects on both biological models. Furthermore, V. faba treated with DAAE showed significant increases in the activities of SOD and CAT enzymes. Mice treated with DAAE exhibited significant elevations in serum biochemical parameters compared to the control group. Histological examination of liver and kidney tissues revealed hepatic degeneration, glomerular shrinkage, and distinct vacuolated tubular epithelial cells. The cotreatment with vitamin C demonstrated a significant protective effect against DAAE-induced cytogenotoxicity. These findings underscore the importance of vitamin C as a protective agent against oxidative stress and cytogenotoxicity induced by DAAE and recommend its use in any DAAE-based preparation.

Chenopodium Ambrosioides Linn Mitigates Bone Loss in Rats with Periodontitis.

Periodontitis is an inflammatory disease that causes bone loss. Some patients do not respond well to the classic treatment and need therapies that minimize bone loss, the main sequel of the disease. Chenopodium ambrosioides L. has stood out due to its anti-inflammatory and anti-oxidative activities. However, no study has yet investigated its effect on periodontitis. This study aimed to evaluate the bone protective effect of Chenopodium ambrosioides L. (CAL) extract on ligature-induced periodontitis model in rats. For this, a pre-clinical assay was performed, using male Wistar rats divided into 3 groups: Naive (N) (n=6), not submitted to any procedure; Saline (SAL) (n=6), submitted to ligature-induced periodontitis and receiving 2 ml/kg of 0.9% saline solution; and CAL extract, which was subdivided into 3 subgroups (n=6/subgroup) receiving the CAL at 3 (CAL3), 10 (CAL10) or 30 mg/kg (CAL30). All agents were given, by oral gavage, 30 min before periodontitis induction and daily until euthanasia (11th day). By then, maxillae were removed for macroscopic, histological, and histometric analyses. Kidneys, liver, and stomach were collected to evaluate the safety of CAL extract. High-performance liquid chromatography (HPLC) assay was used to investigate the flavonoid content in the extract. Chenopodium ambrosioides L. extract at 30mg/kg showed a reduction by 58% in bone loss marked by an increase (+35%) in the number of osteoblasts and a reduction (-51%) on the number of osteoclasts (p< 0.05). No significant alteration in the liver, kidney, or stomach was seen. Rutin was the main flavonoid found. In summary, it was observed that Chenopodium ambrosioides L. extract has shown important anti-inflammatory and bone anabolic and anti-resorptive properties without causing toxicity in the main organs. Rutin, as the main flavonoid of the extract, seems to be responsible for the beneficial effect of this agent.