Chemiluminescence Research Articles

Chemiluminescence-based evaluation of styrene block copolymers' recyclability.

The study presents the chemiluminescence based (CL) evaluation of the recyclability of linear styrene block copolymers. This analysis can provide insight into the material's degradation state and predict its suitability for further recycling cycles, helping to avoid unnecessary energy consumption during processing.The thermal stability of four similar copolymer structures - styrene-isoprene-styrene (SIS), styrene-butadiene-styrene (SBS) copolymers with different styrene/butadiene ratios, and styrene-ethylene-butadiene-styrene (SEBS) - was studied using isothermal and non-isothermal chemiluminescence (CL). The activation energies for oxidative degradation were calculated based on oxidation induction times indicated by the CL intensities evolution. The results, which highlight the influence of molecular structure on stability under aging conditions, as presented by the calculated for the activation energy (kJ mol-1), show the following sequence: SBS (styrene 30%) (117kJ mol-1) ≈ SIS 120 (kJ mol-1) < SBS (styrene 40%) (176kJ mol-1) < SEBS (180kJ mol-1). The CL data were correlated with infrared (IR) spectroscopy and differential scanning calorimetry (DSC) data, providing a comprehensive understanding of the thermal stability and degradation mechanisms during recycling proces. The sequence of the composing units determines the degradation process, with weaker points predominantly attacked in the linear moieties of isoprene, butadiene, and vinyl segments. The experimental data indicate that SIS and SBS (30% styrene) copolymers degrade the fastest likely due to the rapid accumulation of hydroperoxide radicals. The SEBS copolymer also experiences significant degradation, but this occurs at higher temperatures (above 220 ⁰C) and progresses more gradually once it begins. In contrast, the SBS copolymer with 40% styrene content degrades more slowly and exhibits minimal mass loss, primarily due to the formation of less reactive keto degradation products.

Chemiluminescent Fe3O4@Nickel-Cobalt Double Hydroxide Magnetic Core-Shell Nanomaterial as an Analytical Interface for Label-Free CYFRA21-1 Immunosensing.

Cytokeratin 19 fragment (CYFRA21-1) is considered to be a potential marker for the diagnosis and of classification of lung cancer. It is highly desired to develop rapid and highly sensitive label-free chemiluminescence (CL) immunosensors for the detection of CYFRA21-1. In this work, magnetic core-shell nanocomposite Fe3O4@nickel-cobalt double hydroxide (Fe3O4@DH) was used for the first time as a carrier to synthesize N-(4-aminobutyl)-N-ethylisoluminol (ABEI) and gold and silver nanocluster (AuAgNC) bifunctionalized magnetic nanomaterials Fe3O4@DH/AuAgNCs-ABEI. The resulting Fe3O4@DH/AuAgNCs-ABEI demonstrated excellent CL performance, attributed to the exceptional catalytic capability of nickel-cobalt double hydroxide (NiCo-DH) and AuAgNCs, as well as the large specific surface area of NiCo-DH for AuAgNCs and ABEI enrichment. Moreover, Fe3O4@DH/AuAgNCs-ABEI exhibited superior stability compared to Fe3O4-based CL-functionalized magnetic nanomaterials, owing to the presence of NiCo-DH shells. On this basis, a label-free CL immunosensor using CYFRA21-1 antibody-modified Fe3O4@DH/AuAgNCs-ABEI as an analytical interface was constructed for the detection of CYFRA21-1 in the range from 1.0 × 10-13 to 1.0 × 10-8 g/mL. The detection limit of this CL immunosensor was 47.7 fg/mL, 4 orders of magnitude lower than existing CL methods. The CL immunosensor was able to accurately detect the concentration of CYFRA21-1 in serum samples, as evidenced by ELISA results. More importantly, it not only distinguished well between healthy persons and lung cancer patients (90.0% sensitivity and 90.0% specificity), but also effectively distinguished between patients with non-small cell lung cancer and small cell lung cancer (80.0% sensitivity and 86.7% specificity).

DNA Conformation-Regulated Hemin Switch for Lab-on-Chip Chemiluminescent Detection of an Antibody Secreted from Hybridoma Cells.

This work designed a DNA conformation-regulated hemin switch for rapid chemiluminescent (CL) detection of a monoclonal antibodies. This switch was performed with an affinity probe and an inhibition probe, which were conveniently prepared by hybridizing hemin-labeled DNA1 with KHL peptide-labeled DNA2 and binding biotin-labeled DNA3 to streptavidin, respectively. In the absence of the target antibody, streptavidin-DNA3 could hybridize with hemin-DNA1/KHL-DNA2 to release KHL-DNA2, which led to the loss of hemin activity due to the affinity hindrance of streptavidin-DNA3. After the KHL peptide was recognized by the target antibody, the strand replacement hybridization could be inhibited by the bound antibody, which retained the high catalytic activity of hemin overhung on the antibody-bound affinity probe for a CL reaction, leading to a "signal-on" process for CL antibody detection. Using a KHL-specific antibody, anti-proprotein convertase subtilisin/kexin type 9 antibody (PCSK9-Ab), as a target model and common L012-1,2,4-triazole-H2O2 CL system, the designed switch showed a detection range of 10 ng mL-1 to 1 μg mL-1 with a detection limit of 4.16 ng mL-1 (56.2 pM) and a short analytical time of 6.5 min. The proposed quick method could simply be used for lab-on-chip CL detection of PCSK9-Ab in situ-secreted from PCSK9-6E3 hybridoma cells, which showed an accuracy of 90.2% compared with the statistical results from general fluorescence imaging, providing a potential technique for screening specific hybridoma cells.

Tunable Chemiluminescence Kinetics with Hierarchically Structured HKUST-1 and Its Sensing Application for Concanavalin A Analysis.

Introducing novel catalysts is essential for developing chemiluminescence (CL) systems that exhibit sustained and robust emission. Traditional Luminol-H2O2 systems typically feature flash-type CL emission. In this study, we discovered that the porous material HKUST-1 can induce a long-lasting and intense CL emission when combined with Luminol-H2O2. This long-term emission signal can be directly detected by the smartphone. By changing the calcination temperature, a series of microporous and hierarchically porous HKUST-1 materials were prepared as catalysts to adjust the kinetic characteristics of the CL signal of Luminol-H2O2 system from flash-type to glow-type. A systematic investigation into the influence of the central metal and ligand, aperture, and particle size of HKUST-1 on the CL kinetic properties revealed that the pore structure has the most pronounced impact on the dynamics of the Luminol-H2O2 CL reaction. Capitalizing on the intense emission of the HKUST-1-catalyzed Luminol-H2O2 system, we established a CL sandwich immunoassay strategy for concanavalin A (ConA), demonstrating good linearity and low detection limit. This research presents a significant endeavor in modulating the dynamics of CL signal emissions.

Label-free differential chemiluminescent immunosensor based on magnetic nanoparticles CuFe2O4@ABEI-GNPs with dual catalytic sites

BackgroundCancer has become one of the main causes of death globally. The level of tumor markers in serum is correlated with the occurrence of cancer. Carcinoembryonic antigen (CEA) is the most commonly utilized tumor marker for cancer detection. Recently, various analytical technologies have been reported to detect biomarkers. However, developing a simple, sensitive, and noninvasive approach for CEA detection remains challenging in cancer diagnosis. Consequently, there is an urgent need for researchers to carry out innovative approaches for CEA detection. ResultIn this work, copper ferrite nanoparticles (CuFe2O4 NPs) with excellent dispersity and fascinating magnetism have been successfully synthesized. To get CuFe2O4@ABEI-GNPs, ABEI-gold NPs (ABEI-GNPs) were generated on the surface of CuFe2O4 NPs by using N-(4-Aminobutyl)-N-ethylisoluminol (ABEI) as a mild reduction reagent to reduce chloroauric acid tetrahydrate (HAuCl4·4H2O). The CuFe2O4@ABEI-GNPs exhibited a superior chemiluminescence (CL) performance compared with CuFe2O4@ABEI NPs, which was attributed to the synergistic catalysis effects of CuFe2O4 NPs and GNPs. Interestingly, two unique CL emission peaks were observed in the kinetic curve of CuFe2O4@ABEI-GNPs. Furthermore, it was found that the kinetic curve could be regulated by the pH of hydrogen peroxide (H2O2) and a possible CL mechanism was proposed. Owing to the favorable CL properties of CuFe2O4@ABEI-GNPs, a label-free differential immunosensor was fabricated for CEA monitoring using the intensity difference between CL-1 and CL-2. The developed immunosensor exhibited a wide linear range from 0.1 to 5000 pg/mL, and a low detection limit of 0.05 pg/mL. Significance and noveltyThe immunosensor was capable of determining CEA in real samples with simple operation, high accuracy, and good sensitivity. This study introduces a novel approach for developing CL functionalized materials, which have broad application potential in bioassays. The proposed differential method could serve as a novel tool for determining CEA in the diagnosis of clinical cancer.

Investigation of the chemiluminescence behavior of Ti3C2 MXene quantum dots as a sensing platform for uric acid

The current study explores the application of Ti3C2 MXene quantum dots (MQDs) as a chemiluminescent (CL) probe to determine uric acid (UA) in biological samples. The MQDs sensor was synthesized using ultrasonic-assisted hydrothermal method and characterized through Fourier transform infrared spectroscopy, Raman spectroscopy, scanning electron microscopy, energy dispersive X-ray analysis, high-resolution transmission electron microscopy, and X-ray diffraction. The presence of MQDs significantly enhances the ultra-weak Fe(II)–S2O82− CL system. Subsequently, quenching of the probe’s CL signal was observed in the presence of UA. Thus, the designed CL sensor offers an excellent analytical capability for determining UA in the 0.15–2.5 µM range, with a limit of detection of 0.093 µM. The method was effectively used to analyze UA in human serum and urine, yielding recovery rates between 94.4 % and 108.3 % with relative standard deviations (RSD) ranging from 1.9 % to 5.9 %.

Persistent chemiluminescence-based nanosensor for portable point-of-care testing of H2O2.

Anovel glow-type chemiluminescence (CL)-based nanosensing system was developed for sensitive and rapid point-of-care testing (POCT) of H2O2 in food. CuSe nanoparticles (CuSeNPs) have excellent peroxidase-like activity. After being modified with thiols of 4-mercaptophenylboronic acid (MBA) (CuSeNPs@MBA), luminol can be catalyzed to produce long-lasting CL in the presence of H2O2. The possible reason for the long-lasting glow-type CL behavior was explored. Under the optimized condition, H2O2 can be sensitively detected with improved repeatability. The limit of detection isas low as 0.30 μM. To meet the requirement of in situ and outside of laboratory detection, a 3D-printed portable device was designed which can eliminate the environmental interference to improve detection accuracy. The developed multifunctional platform also has the advantages of simple operation and low cost, suggesting its great potential for applications in food and agricultural fields.

Flow injection chemiluminescence determination of lambda-cyhalothrin and clodinafop-propargyl in freshwater samples

ABSTRACT A flow injection with chemiluminescence (CL) detection system applying tris-2,2′-bipyridylruthenium(II)–potassium permanganate ([Ru(bpy)3]2+–KMnO4) CL reaction was established to determine lambda-cyhalothrin (LCT) and clodinafop-propargyl (CP) pesticides in freshwater samples. Experimental parameters were optimised, and solid phase extraction was employed as phase separation techniques. Linearity of calibration curves for the standard solutions ranges of LCT and CP were of 0.001–1.0 mg/L (y = 1230.3x +23.895, R 2 = 0.9998 (n = 7)) and 0.001–2.0 mg/L (y = 680.12x +18.5, R 2 = 0.9998 (n = 9)), respectively. The respective limits of detection (LODs) and limits of quantification (LOQs) for LCT and CP were found as 3.4 × 10−4 and 3.5 × 10−4 mg/L (3σ blank) and 9.3 × 10−4 and 9.6 × 10−4 mg/L (10σ blank). The injection throughput was 115 h−1. As a consequence of applying tests of significance, the obtained results by the suggested method were not significantly different from those of the reported methods at a 95% confidence level. Recoveries for LCT and CP were achieved over the ranges of 93.8–108% (relative standard deviation (RSD) = 1.7–3.4%) and 93.4–108.2% (RSD = 2.2–3.5%), respectively. The most possible CL reaction mechanism was investigated and formulated.

Near-Infrared Unimolecular Chemiluminescence Probes for Deep-Tissue Imaging.

Chemiluminescence (CL) imaging has emerged as a promising optical imaging technique due to minimal background autofluorescence and being excitation-free. However, the emission of most chemiluminescent probes was concentrated in the visible light region, which limited the tissue penetration. Although some NIR chemiluminescence probes have been reported based on the chemiluminescence resonance energy transfer (CRET) strategy, the energy loss was inevitable. Thus, it is crucial to develop near-infrared (NIR) unimolecular probes with direct chemiluminescence. Herein, we propose a strategy of increasing conjugation for designing and synthesizing novel NIR chemiluminescence unimolecular probes that consist of luminol, electron acceptor, π-bridge, and electron donor. Luminol was conjugated to the unimolecular backbone to produce direct NIR chemiluminescence. Notably, the direct CL mechanism of probes was investigated. Compared with CRET-based chemiluminescence, this direct CL was more advantageous to immediately convert the chemical energy into chemiluminescence, avoiding energy degradation. Furthermore, the corresponding nanoparticles with great biosafety were prepared by self-assembly with amphiphilic DSPE-PEG. Especially, TTBL@PEG-NPs with NIR-I emission were successfully used in the sensitive in vivo chemiluminescence imaging of various inflammation models, such as peritonitis, ear swelling, and colitis. This study paves the way for the design of NIR unimolecular chemiluminescence probes and deep-tissue imaging.

Self-Illuminating Copper-Luminol Coordination Polymers for Bioluminescence Imaging of Oxidative Damage.

Timely detection of reactive oxygen species (ROS) accumulated during inflammation is essential for an early disease diagnosis. Compared to fluorescence probes with limited sensitivity and accuracy, chemiluminescence (CL) imaging offers the potential for highly sensitive molecular visualization of ROS by minimizing background interferences. However, the development of bright and easily manufacturable CL probes for ROS imaging remains challenging. In this study, a novel chemiluminescent nanoprobe named Cu-Lum@NPs for ROS imaging in inflammation was synthesized by using a one-step solvothermal method. The Cu-Lum@NPs, which are composed of coordination polymers containing copper ions and luminol (Lum), demonstrate intrinsic peroxidase-like activity that relies on Cu(I) as the catalytic active center to initiate the Fenton reaction. This catalytic process facilitates the decomposition of hydrogen peroxide (H2O2) into hydroxyl radicals (•OH) and superoxide anion radicals (O2•-), leading to the oxidation of Lum and inducing strong luminescence. Cu-Lum@NPs, displaying nanozyme characteristics, were observed to accelerate and enhance the ROS-responsive luminescence (10-1600-fold in solution and over 100-fold in neutrophils) and notably extend persistent luminescence. The Cu-Lum@NPs allowed for CL imaging of endogenous ROS in living cells and animals with an outstanding signal-to-noise ratio exceeding 96 and facilitated oxidative damage luminescence imaging for tissue-specific detection. The study presents Cu-Lum@NPs, a highly sensitive and easily manufacturable chemiluminescent nanoprobe for ROS imaging both in vitro and in vivo, exhibiting enhanced luminescence and prolonged persistence for ROS-related disease detection.

Sulfur doped hollow cobalt–nitrogen–carbon oxidase-mimicking nanozyme enable sensitive chemiluminescence sensing serum total antioxidant capacity

Heteroatom doping is one of most important methodology for heightening catalytic activity of a nanozyme through modulating electronic configuration and coordinated environment of metal atoms. Here, a nitrogen/sulfur co-doped cobalt-decorated hollow carbon structure (CoS/NC) was fabricated through a simple and manageable template-assisted synthetic strategy, following high-temperature carbonization treatment. Remarkably, the CoS/NC has superior oxidase-mimicking activity than its counterparts and displays excellent catalytic action on the chemiluminescence (CL) reaction between luminol and dissolved oxygen (O2). The CoS/NC nanozyme-based CL system is highly sensitive to physiologically relevant antioxidants of both species including single electron transfer and hydrogen atom transfer. The detection limits can be reached as low as 3 nmol·L−1 ascorbic acid, 30 nmol·L−1 uric acid, 16 nmol·L−1 glutathione, and 28 nmol·L−1 cysteine. The total antioxidant capacity (TAC) of human serums has been determined in order to assess the feasibility of the CL system. The results of serum TAC, with ascorbic acid as an equivalent model, show a good consistency with those obtained by the standard CUPRAC method. This work not only benefits the rational design of a high catalytic functionality nanozyme, but also constructs a robust TAC detection platform for monitoring oxidative stress-related diseases.

Long-Lasting Chemiluminescence Based on Functionalized Multicolor Protein Capsules for Multiple Visualization Detection of Avian Influenza Virus Biomarkers.

Long-lasting chemiluminescence (CL) emissions are necessary for improving the detection accuracy and expanding the application scope. Here, we have synthesized three oil-in-water (O/W) multicolor protein capsules (LCBA, F/LCBA, and RB/F/LCBA) using a simple ultrasound method and have engineered specific target-triggered catalytic hairpin assembly on their surface and chemiluminescence resonance energy transfer inside. Consequently, three multicolor capsules exhibit excellent structural stability, generate blue-, green-, and red-colored emissions when reacting with H2O2, have long-lasting CL emission over 1 h, and successfully achieve the accurate multiple visualization detection of avian influenza virus subtype targets. Without the need for complex instruments and analysis procedures, the CL imaging assays can be carried out and recorded with a common smartphone. The detection limits for visualizing H1N1, H7N9, and H5N1 are 5.5, 7.6, and 9.0 pM, respectively. There is a linear range between 20.0 and 625 pM and excellent selectivity against interfering DNA. Furthermore, visualization detection has been successfully applied for the detection of H1N1, H7N9, and H5N1 in healthy human serum samples. With these merits, this facile, ultrasensitive, and multiple visualization sensor has potential applications in point-of-care testing and early diagnosis.

Comparison of Dietary Supplementation with Krill Oil, Fish Oil, and Astaxanthin on an Experimental Ethanol-Induced Gastric Ulcer Model: A Biochemical and Histological Study

Background/Objectives: Despite advances in ulcer treatment research, the search for new, safe, and effective strategies for preventing and treating ulcer diseases persists. Methods: In this study, the protective effects of dietary supplementation with krill oil (KO), fish oil (FO), and astaxanthin (ASX) on an ethanol-induced gastric ulcer model were compared during biochemical and histological observations. Sprague–Dawley (n = 64) rats randomly divided into four groups—normal control (vehicle), KO, FO, and ASX groups—received the supplements via the orogastric route at a rate of 2.5% (v/w) of their daily feed consumption for 4 weeks. Then, ulcer induction was performed with ethanol. Results: The ulcer group showed increased levels of malondialdehyde (MDA), chemiluminescence (CL), and myeloperoxidase (MPO) activity and decreased levels of glutathione in the gastric tissues. While KO, FO, and ASX supplementation decreased chemiluminescence levels in the ulcer group, only ASX supplementation decreased MDA levels and MPO activity. Conclusions: In conclusion, supplementation with KO or FO has a similar protective effect against ethanol-induced ulcer damage, as it inhibits ROS formation and reduces lipid peroxidation. However, ASX supplementation has a higher protective effect than KO or FO supplementations against experimental ethanol-induced gastric lesions in rats, as it inhibits ROS formation and reduces neutrophil infiltration and lipid peroxidation.

Novel sulphur-selective method for simultaneous determination of thiram and asomate based on carbon dots and its application in fruits

Thiram (THI) and asomate (ASO) are frequently detected agrochemicals in food samples due to widespread use as fungicides. However, their detection suffers from matrix interference, or the necessity of derivatization before measurement. Integrating HPLC with a post-column carbon dots (CDs)–enhanced chemiluminescence (CL) system makes it possible to simultaneously separate and selectively detect ASO and THI in fruit samples free of derivatization and deep purification. The studies on mechanisms demonstrated that CD intermediates of anionic radical were responsible for the remarkable CL enhancement of ASO or THI. The CL intensity is directly proportional to the fungicide concentration, with the ranges of linearity for ASO and THI being 5–250 μg·L−1 (R2 = 0.9993) and 4–200 μg·L−1 (R2 = 0.9986), respectively, and the corresponding limits of detection (S/N = 3) being 1.61 and 1.33 μg·L−1, respectively. The developed method enables the exploration of the novelly sulphur-targeted CL application based on carbon nanomaterials.

Cu3(HITP)2 with peroxidase- and ascorbic acid oxidase-like catalytic activity for fluorescence/chemiluminescence sensing of ascorbic acid

Nanomaterials with intrinsic enzyme mimicking activity have achieved widespread application. However, developing novel nanomaterials with multienzyme mimicry activity remains challenging. Herein, Cu3(HITP)2 (HITP = 2,3,6,7,10,11-hexaiminotriphenylene) with ascorbic acid oxidase (AAO)- and peroxidase (POD)-like activity are successfully synthesized. Cu3(HITP)2 exhibits excellent AAO-like activity and can specifically catalyze the oxidation reaction of ascorbic acid (AA). Dehydroascorbic acid (DHAA) obtained from the oxidation of AA is allowed to react with nonfluorescent o-phenylenediamine (OPD) to form 3-(1,2-dihydrox-yethyl) furo[3,4-b]quinoxaline-1-one (DFQ) with strong fluorescence emission. Moreover, Cu3(HITP)2 is able to catalyze the chemiluminescence (CL) reaction of ABEI-H2O2 to generate a strong and glow-type emission based on its POD activity. Inspired by the multienzyme mimicry activity of Cu3(HITP)2, the simple and sensitive fluorescence and chemiluminescence sensing platforms are successfully constructed and applied for the detection of AA. The sensors show high sensitivity and excellent selectivity. We believe that this multienzyme mimicry activity nanomaterial not only can be used to construct the multiple-mode biosensing platform, but also enables the extensive applications in the fields of biomedicine, energy, and environment.

Polyoxometalate-induced hydrogel with in situ nano/bioenzyme cascade reaction generating intensive and long-lasting glow-type chemiluminescence

In this study, we introduce a straightforward one-step, in-situ strategy for the generation of long-lasting and intensive glow-type chemiluminescence (CL) for glucose sensing in biological samples. The approach involves the utilization of polyoxometalate (POM)-induced alginate hydrogel, which effectively encapsulates both the catalysts (POM and glucose oxidase (GOx)) and the CL reagent (luminol) in solution. Leveraging the peroxidase-like nanozyme properties of POM and the bioenzyme GOx, embedded within the nano-sized pores of the hydrogel, we achieve intensive and persistent CL emission lasting over 16 h. The POM-hydrogel-based CL system demonstrates remarkable storage stability and reproducibility, and it can be applied to quantitatively determine serum glucose with high accuracy. Our study indicates that the POM-hydrogel platform, supporting and confining nano/bioenzyme cascade reactions, holds great promise for the establishment of glow-type CL systems, which could be of great value in various fields of CL biosensing and imaging.

Magnetic nanoparticle-decorated metal organic frameworks for chemiluminescence detection of glutathione.

A chemiluminescence (CL) method for determination of glutathione (GSH) was developed with magnetic nanoparticle-decorated metal organic frameworks (Fe3O4 NPs@Cu-TATB). The composite material was synthesized via a hydrothermal method and glutathione (GSH) can be detected by both visual and chemiluminescence (CL) methods. The synthesized Fe3O4 NPs@Cu-TATB exhibited excellent catalytic activity in the luminol-H2O2 CL system. The mechanism revealed that three types of oxygen-containing radicals (ROS) were generated in this system. As GSH can reduce the catalytic effect of generated ROS radicals, the inhibiting CL signal was produced in the Fe3O4 NPs@Cu-TATB-luminol-H2O2 system. Based on the established CL system, the detection limits for GSH using CL and visual methods were found to be 0.3 µM and 0.7 µM, respectively. This low-cost and convenient detection method can be applied to the determination of GSH content in human blood.

Amino-rich silicon quantum dots as efficient activator with intrinsic chemiluminescence for the detection of peroxydisulfate

The specific detection of peroxydisulfate (S2O82−, PDS) is significant and challenging due to the rapid development of PDS-related technologies and their widespread application in multiple fields. However, traditional analytical methods are mainly based on their strong oxidizing properties, making it difficult to simultaneously achieve specific identification and high sensitivity for PDS detection in complex water environments. Here, we purposely prepared amino-rich SiQDs (N-SiQDs) as an effective catalyst and introduced H2O2 acts as a co-reactant for PDS activation and determination with strong intrinsic chemiluminescence (CL) emission. High yield of reactive active oxygen (mainly O2˙− and ˙OH) were generated during CL process, which trigger electron-hole annihilation between the N-SiQDs˙+ and N-SiQDs˙− accounted for extraordinary CL emission. On this basis, a new CL assay for PDS detection was fabricated with broad linear range of 5 × 10−7M-5 × 10−5 M and low detection limit (3.2 × 10−7 M). Due to the absence of SO4˙− involvement during CL emission, the sensing platform is sensitive enough, satisfactory selectivity and does not respond to transition-metal ions and inorganic anions that have interferences in the PDS CL sensors reported before. This work not only deepens insight into the mechanisms of nanomaterials assisted PDS activation but also provides a new perspective on the modified metal-free QDs CL probe for chemical species detection.

An ingenious chemiluminescence sensing strategy for recalcitrant triphenyl phosphate based on oxidant-free UV-activated MIL-100(Fe) gel system

BackgroundOrganophosphate flame retardants (OPFRs) are notorious emerging contaminants threatening the environment and human health. Triphenyl phosphate (TPHP), which has an extremely serious biotoxicity, is a typical harmful OPFR. Due to its wide use, TPHP has been discovered in various environmental mediums. Moreover, it is pretty recalcitrant to the removal process, resulting in the need for a technique to understand it better. Hence, accurate and quick discrimination of TPHP in the environment is critical to further evaluate its potential effect on ecosystems and human health. ResultsAn ingenious oxidant-free chemiluminescence (CL) sensor based on the oxidant-free UV/MIL-100(Fe) gel system was established for TPHP detection. The oxidation of luminol in the UV-activated MIL-100(Fe) gel has resulted in remarkable CL emission, which is contributed by reactive oxygen species (ROS) generated by it. Notably, the CL intensity was inhibited significantly after introducing TPHP. An investigation into the mechanism underlying the effect of CL suppression demonstrated that TPHP competed with luminol to consume ROS from UV-activated MIL-100(Fe) gel, contributing to CL inhibition. The subsequent sensing performance experiments demonstrated the advantages of environmentally friendly, economic efficiency, user-friendly operation, rapid determination, potential for compact size, high selectivity, and sensitivity. Additionally, these investigations confirmed the low limit of detection (210 ng L−1) and wide linear range (10–1000 μg L−1). SignificanceIn this paper, a green, economical, and oxidant-free CL sensing strategy for TPHP has been established. It has the advantage of being rapid, having the potential for compact size, high selectivity, and sensitivity. This ingenious method has promising applications in real-time and online environmental monitoring, and it paves the way for the rapid and environmentally friendly identification of emerging contaminants that are structurally stable and recalcitrant to remove.

Enhanced chemiluminescence by carbon dots for rapid detection of bisphenol A and nitrite

Herein, a novel chemiluminescence (CL) sensor was successfully developed based on chlorine doped carbon dots (Cl/CDs) for the rapid determination of bisphenol A (BPA) and nitrite. The Cl/CDs were synthesized through a hydrothermal method, using ascorbic acid as the precursor and hydrochloric acid as the dopant. It was found that Cl/CDs significantly enhanced the CL intensity of the acid-KMnO4 system, while BPA and nitrite quenched the CL intensity of the Cl/CDs-sensitized acid-KMnO4 system. Under optimal conditions, BPA exhibited a linear detection range of 0.05–10 μM, with limits of detection (LOD) and quantification (LOQ) of 0.86 nM and 2.8 nM, respectively. Nitrite showed a linear detection range of 0.7–100 μM, with LOD and LOQ of 22.5 nM and 75 nM, respectively. The CL sensor was successfully use to determine BPA in water samples and nitrite in pickles, ham and celery, with spike recovery rates of 96.3 %–104.8 % and 96.0 %–104.9 %, respectively.