The olive fruit fly, Bactrocera oleae (Rossi) (Diptera, Tephritidae) is the most devastating insect pest possessing severe economic threats for the olive growers in Jordan. Therefore, the current study aimed at determining the effect of insecticidal applications against B. oleae on olive quantity, as well as physical (refractive index, fruit weight, diameter and volume, and weight of seed and flesh, as well as oil percentage) and chemical (olive oil acidity and peroxide value parameters). The experiment was conducted using Nabali olive cultivar in Karak District-Jordan in the year 2016. Three insecticides; Karate, Fytoclean, and Dursban used in the spraying operations, and trees were sprayed twice. Insecticidal spraying significantly (P<0.05) reduced olive oil acidity, peroxide value, and refractive index, and increased olive fruit’s weight, diameter and volume, and seed, and flesh weight compared to the untreated trees. In conclusion, the application of insecticides against B. oleae improved the olive quantity and tested quality parameters.