Chemical Methodology Research Articles

An Investigation into the Larvicidal Activity of Biologically Synthesized Silver and Copper Oxide Nanoparticles Against Mosquito Larvae.

This study is primarily focused on the synthesis of silver and copper oxide nanoparticles utilizing the extract of Ipomoea staphylina plant and their larvicidal activity against specific larvae. Notably, Anopheles stephensi and Aedes aegypti are significant disease vectors responsible for transmitting diseases such as malaria, dengue fever, Zika virus, and chikungunya (Anopheles stephensi), and dengue and Zika (Aedes aegypti). These mosquitoes have a substantial impact on urban areas, influencing disease transmission dynamics. In an effort to control these larvae, we have pursued the synthesis of a herbal-based nanomedicine derived from I. staphylina, a valuable herb in traditional medicine. Our successful synthesis of silver and CuO nanoparticles followed environmentally sustainable green chemistry methodologies. The I. staphylina plant extract played a dual role as a reducing agent and dopant, aligning with principles of sustainability. We employed X-ray diffraction (XRD) analysis to validate the nanoparticle structure and size, while field-emission scanning electron microscopy (FE-SEM) revealed well-defined nanostructures. Elemental composition was determined through energy-dispersive X-ray (EDX) analysis, and UV-visible spectroscopy provided insights into the bandgap energy (3.15 eV for silver, 1.2 eV for CuO nanoparticles). These nanoparticles exhibited robust larvicidal activity, with CuO nanoparticles surpassing silver nanoparticles in terms of LC50 and LC90 values. Moreover, the developmental toxicity of CuO and Ag NPs was evaluated in zebrafish embryos as part of non-target eco-toxicological studies conducted in a standard laboratory environment. These findings underscore the potential utility of these nanoparticles as highly effective and environmentally friendly natural pesticides, offering cost-effectiveness and ecological benefits.

Advanced Approaches in Dyeing and Printing Wastewater Treatment: A Comparative Study of Physical and Chemical Methods

This thesis presents a comprehensive analysis of various physical and chemical methodologies for treating dyeing and printing wastewater, which is a significant environmental concern in the textile industry. It delves into physical techniques like ultrafiltration, nanofiltration, reverse osmosis, and magnetic separation, evaluating their efficiency in removing contaminants and the challenges faced, such as membrane fouling and the requirement for high-quality magnetic seeds. The study also explores chemical methods like electrochemical oxidation and photocatalytic oxidation, highlighting the advancements in electrode materials and the use of TiO2 catalysts in degrading pollutants. A comparison is made between the effectiveness, operational constraints, and economic viability of these methods, emphasizing the need for optimizing existing technologies and developing hybrid systems that integrate the strengths of individual techniques. This research aims to contribute to the ongoing efforts on enhancing wastewater treatment efficiency, reducing environmental impact, and promoting sustainable practices in the textile industry. This work serves as a reference for future research on wastewater treatment, offering insights into developing more effective, cost-efficient, and eco-friendly solutions.

Simple and Cost-Effective Quantum Dot Chemodosimeter for Visual Detection of Biothiols in Human Blood Serum.

An emission "turn-off" chemodosimeter for the naked-eye detection of biothiols using silica-overcoated cadmium selenide quantum dots is developed. Hole scavenging by the thiol group of cysteine, homocysteine, or glutathione on interaction with quantum dots resulted in an instant and permanent emission quenching under physiologically relevant conditions. Also, the emission suppression is so specific that thiols and substituted thiols (methionine and cystine) can easily be distinguished. A pilot experiment for the visual detection of serum thiols in human blood was also conducted. Densitometry analysis proved the potential of this system as a new methodology in clinical chemistry and research laboratories for routine blood and urine analyses using a simple procedure. This method enables one to visually distinguish biothiols and oxidized biothiols, whose ratio plays a crucial role in maintaining "redox thiol status" in the blood.

On the use of aromatic-functionalized reduced graphene oxide to confine corrosive ions in nanocomposite coatings

A simple and fast chemical methodology to obtain reduced graphene oxide (GO) nanoplatelets functionalized with aromatic groups and their anticorrosive performance in an epoxy resin matrix is disclosed for the first time. Through a microwave-assisted method, GO was functionalized with triethoxyphenylsilane (TEPS) within 10 min to obtain Phenyl-GO nanoplatelets. The remaining oxygen-centered groups of the nanoplatelets were removed in a second step under a microwave-assisted reduction for 10 min to obtain Phenyl-rGO nanoplatelets. The proposed methodology produced highly exfoliated, hydrophobic, and thermally stable functionalized graphene nanoplatelets. Phenyl-rGO nanoplatelets were used as an additive (0.5 wt%) within an epoxy resin, resulting in Epoxy Resin/Phenyl-rGO nanocomposite, which was used as an anti-corrosive coating on A36 mild steel. The physicochemical properties of the epoxy resin were improved by the Phenyl-rGO addition, such as density, hardness, adhesion, hydrophobic behavior, and anticorrosive performance. The impedance module was increased up to 5 magnitude orders, reaching up to 1.0 × 106 Ω·cm2, and the corrosion rate (CR) was decreased down to 2 magnitude orders (from 1.67 down to 0.01 mm/year). Moreover, the non-polar character of the Phenyl-rGO, allowed the migration of the reduced nanoplatelets to the interphase and surface of the coating, resulting in nanoplatelets densely packed. The tortuous pathway of the corrosive species, generated by the interaction between nanoplatelets, results in a nanocomposite coating that is able to confine such species and prevent corrosion of the metallic surface.

Computational investigation of HO•-induced atmospheric transformation and ecological risk assessment of 2-methyl-4-chlorophenoxyacetic acid

2-Methyl-4-chlorophenoxyacetic acid (MCPA) is a pervasive phenoxyacetic acid herbicide, frequently identified in environmental matrices. This study undertakes a systematic investigation of the atmospheric environmental transformation behavior and potential ecological risks of hydroxyl radical (HO•) induced MCPA for the first time, employing computational chemistry and computational toxicology methodologies. Our findings indicate that the initial degradation of MCPA can be triggered by both HO•-addition and hydrogen-abstraction reactions, with the latter being more likely to occur. The primary reaction pathways were identified as R2, R3, R8, and R13. The total rate constant for the gas-phase reaction of MCPA with HO• was calculated as 8.59 × 10−12 cm3 molecule −1 s−1 at 298 K. This total rate constant displayed a distinct positive temperature dependence within the temperature range of 250–375 K. Structures of 25 transformation products, which include 7 experimentally observed compounds, were determined from further reactions of crucial intermediates. The toxicity assessment of MCPA and its transformation products indicated that most of the hydroxyl oxidation products exhibit toxicity to aquatic organisms and possess mutagenic, developmental toxic, non-genotoxic carcinogenic, and skin irritating or corrosive properties. This research contributes to a comprehensive understanding of the transformation mechanisms, kinetics, and environmental repercussions of gas-phase MCPA, enhancing our ability to predict and mitigate its ecological impact.

Late-Stage Functionalization of Living Organisms: Rethinking Selectivity in Biology.

With unlimited selectivity, full post-translational chemical control of biology would circumvent the dogma of genetic control. The resulting direct manipulation of organisms would enable atomic-level precision in "editing" of function. We argue that a key aspect that is still missing in our ability to do this (at least with a high degree of control) is the selectivity of a given chemical reaction in a living organism. In this Review, we systematize existing illustrative examples of chemical selectivity, as well as identify needed chemical selectivities set in a hierarchy of anatomical complexity: organismo- (selectivity for a given organism over another), tissuo- (selectivity for a given tissue type in a living organism), cellulo- (selectivity for a given cell type in an organism or tissue), and organelloselectivity (selectivity for a given organelle or discrete body within a cell). Finally, we analyze more traditional concepts such as regio-, chemo-, and stereoselective reactions where additionally appropriate. This survey of late-stage biomolecule methods emphasizes, where possible, functional consequences (i.e., biological function). In this way, we explore a concept of late-stage functionalization of living organisms (where "late" is taken to mean at a given state of an organism in time) in which programmed and selective chemical reactions take place in life. By building on precisely analyzed notions (e.g., mechanism and selectivity) we believe that the logic of chemical methodology might ultimately be applied to increasingly complex molecular constructs in biology. This could allow principles developed at the simple, small-molecule level to progress hierarchically even to manipulation of physiology.

From Waste to Value: Recent Insights into Producing Vanillin from Lignin.

Vanillin, one of the most widely used and appreciated flavoring agents worldwide, is the main constituent of vanilla bean extract, obtained from the seed pods of various members belonging to the Orchidaceae family. Due to the great demand in the food confectionery industry, as well as in the perfume industry, medicine, and more, the majority of vanillin used today is produced synthetically, and only less than one percent of the world's vanilla flavoring market comes directly from the traditional natural sources. The increasing global demand for vanillin requires alternative and overall sustainable new production methods, and the recovery from biobased polymers, like lignin, is an environmentally friendly alternative to chemical synthesis. The present review provides firstly an overview of the different types of vanillin, followed by a description of the main differences between natural and synthetic vanillin, their preparation, the market of interest, and the authentication issues and the related analytical techniques. Then, the review explores the real potentialities of lignin for vanillin production, presenting firstly the well-assessed classical methods and moving towards the most recent promising approaches through chemical, biotechnological and photocatalytic methodologies, together with the challenges and the principal issues associated with each technique.

A New Approach for Element Characterization of Grapevine Tissue with Laser-Induced Breakdown Spectroscopy

The determination of grape quality parameters is intricately linked to the mineral composition of the fruit; this relationship is increasingly affected by the impacts of climate change. The conventional chemical methodologies employed for the mineral quantification of grape tissues are expensive and impracticable for widespread commercial applications. This paper utilized Laser-Induced Breakdown Spectroscopy (LIBS) to analyze the mineral constituents within the skin, pulp, and seeds of two distinct Vitis vinifera cultivars: a white cultivar (Loureiro) and a red cultivar (Vinhão). The primary objective was to discriminate the potential variations in the calcium (Ca), magnesium (Mg), and nitrogen (N) concentrations and water content among different grape tissues, explaining their consequential impact on the metabolic constitution of the grapes and, by extension, their influence on various quality parameters. Additionally, the study compared the mineral contents of the white and red grape cultivars across three distinct time points post veraison. Significant differences (p < 0.05) were observed between the Loureiro and Vinhão cultivars in Ca concentrations across all the dates and tissues and for Mg in the skin and pulp, N in the pulp and seeds, and water content in the skin and pulp. In the Vinhão cultivar, Ca differences were found in the pulp across the dates, N in the seeds, and water content in the skin, pulp, and seeds. Comparing the cultivars within tissues, Ca exhibited differences in the pulp, Mg in the skin and pulp, N in the pulp and seeds, and water content in the skin, pulp, and seeds. These findings provide insights into the relationship between the grape mineral and water content, climatic factors, and viticulture practices within a changing climate.

Antibody Production, Immunoassay Establishment, and Specificity Study for Flunixin and 5-Hydroxyflunixin.

Flunixin (FLU) is a nonsteroidal drug that is widely used in animals, causing severe drug residues in animal-derived foods and environment. The development of antibody-based rapid immunoassay methods is of great significance for the monitoring of FLU and its metabolite 5-hydroxyflunixin (5-FLU). We prepared monoclonal antibodies (mAbs) with different recognition spectra through FLU-keyhole limpet hemocyanin conjugates as immunogen coupled with antibody screening strategies. mAb5E6 and mAb6D7 recognized FLU with high affinity, and mAb2H5 and mAb4A4 recognized FLU and 5-FLU with broad specificity. Through evaluating the recognition of these mAbs against more than 11 structural analogues and employing computational chemistry, molecular docking, and molecular dynamics methodologies, we preliminarily determined the recognition epitope and recognition mechanism of these mAbs. Finally, an indirect competitive enzyme-linked immunosorbent assay for FLU based on mAb6D7 was developed, which exhibited limits of detection as low as 0.016-0.042 μg kg -1 (L-1) in milk and muscle samples.

Novel Sustainable Alternatives for the Study of the Chemical Composition of Cork

Cork is a natural and renewable material extracted from the cork oak Quercus suber L. (1) Background: The cellular structure and chemical composition of the plant wall give cork its physical and mechanical properties. Chemically, the composition of cork is principally dominated by the presence of suberin as the main structural cell wall component and affected by its close association with other components. The usual chemical analysis of cork has been partially adapted from wood and other lignocellulosic analytical methods and it is a method with extensive procedures. This study aims to (i) find quicker and more sustainable methods for testing the cork chemical composition, (ii) conduct extraction tests using alternative techniques, (iii) optimize alternative methodologies, and (iv) validate the proposed sustainable methodologies. (2) Methods: Microwave-assisted extraction (MAE) and accelerated solvent extraction (ASE) are explored as alternative systems to the traditional method. The percentages of the extractives obtained via MAE are lower than the percentages obtained by means of the classical composition. (3) Results: However, the percentages of suberin are very similar in both methods: 46.39% in the case of the classical composition and 45.11% in the case of microwave-assisted extraction. No significant differences are observed between the content of the extractives in the dichloromethane, ethanol, and water obtained via ASE and the results obtained with the classical methodology. (4) Conclusions: MAE and ASE are faster methods; they use less solvents and provide more reproducible results than the classical chemical composition methodology. These data pave the way for novel sustainable alternatives relative to the studies of the chemical composition of cork.

Evaluating students’ computation skills in learning amount of substance based on SOLO taxonomy in secondary schools

ABSTRACT In upper-secondary chemistry education, the concept of ‘Amount of Substance’ occupies a central position within the computation framework. However, comprehending this concept often poses a challenge for students. To address this issue, our study aimed to design and implement a computation skills test to assess the varying levels of students’ computation skills in understanding Amount of Substance. The test was administered to a total of 214 first-year students at the secondary schools. To analyse the test scores and unveil students’ comprehensive and mastery levels of computation skills, the SOLO Taxonomy was employed for scoring the levels. Additionally, interviews were conducted to identify the underlying reasons for their learning difficulties. Findings revealed that the majority of students demonstrated proficiency in solving relatively straightforward problems and applying basic formulas. Only a small fraction of students reached the advanced relational level, showcasing their ability to apply chemical concepts and methodologies to solve complex problems. Moreover, students exhibited varying degrees of proficiency when dealing with different types of knowledge and problems associated with computations involving solutions and the quantification of microscopic particles. Interviews uncovered students’ major learning difficulties. These findings inform the teachers’ teaching of Amount of Substance in chemistry education.

Targeting ion channels with ultra-large library screening for hit discovery.

Ion channels play a crucial role in a variety of physiological and pathological processes, making them attractive targets for drug development in diseases such as diabetes, epilepsy, hypertension, cancer, and chronic pain. Despite the importance of ion channels in drug discovery, the vastness of chemical space and the complexity of ion channels pose significant challenges for identifying drug candidates. The use of in silico methods in drug discovery has dramatically reduced the time and cost of drug development and has the potential to revolutionize the field of medicine. Recent advances in computer hardware and software have enabled the screening of ultra-large compound libraries. Integration of different methods at various scales and dimensions is becoming an inevitable trend in drug development. In this review, we provide an overview of current state-of-the-art computational chemistry methodologies for ultra-large compound library screening and their application to ion channel drug discovery research. We discuss the advantages and limitations of various in silico techniques, including virtual screening, molecular mechanics/dynamics simulations, and machine learning-based approaches. We also highlight several successful applications of computational chemistry methodologies in ion channel drug discovery and provide insights into future directions and challenges in this field.

A comprehensive summary on the enhancement of properties of concrete with recycled polypropylene materials

AbstractThe scientific community is currently confronted with critical problems in providing a healthy world for future generations. The study for sustainable plastic recycling methods is difficult for a variety of purposes. Indeed, during the previous century, the widespread usage of plastic materials has resulted in vast amounts of long‐lasting waste that has remained largely unaddressed by effective collection and disposal policies. Packaging materials are responsible for the majority of this waste which also includes polypropylene materials. However, environmental concerns imposed a new trend in the previous decade that brought this research under focus, as seen by the growing number of linked publications. For the recycling of polymeric plastic materials, several chemical and mechanical methodologies have been proposed. Due to the low ultimate tensile strain of concrete, plastic waste fiber materials have been used in concrete in recent times as the reinforced concrete components often function with cracks during the serviceability phase. The bonding strength and stress variation during flexure, toughening effect of a hybrid combination of steel and recycled polypropylene fibers on high‐strength concrete are investigated. Polypropylene's compressive and tensile strength, good dielectric characteristics, and acid and alkali resistance have all been observed in various studies. Hence the current review focus on the past researches on utilization of recycled polypropylene in concrete for the enhancement of its physical and mechanical properties.

The biological applications of IPN hydrogels.

Interpenetrating polymer network (IPN) hydrogels are an adaptable category of materials, exhibiting remarkable promise for various biological applications due to their distinctive structural and functional attributes. This review delves into the synthesis of IPN hydrogels through both physical and chemical methodologies, elucidating how these techniques allow for precise tailoring of mechanical properties, swelling behaviour, and biocompatibility. We conducted an extensive literature review by searching well-established online research databases for articles published since 2009 to gather comprehensive data on IPN hydrogels. Our review highlights several critical applications of IPN hydrogels in the biomedical field; i) Tissue engineering: IPN hydrogels are evaluated for their capacity to emulate the extracellular matrix, making them excellent scaffolds for tissue engineering; ii) Controlled drug release: The ability of IPN hydrogels to modulate drug release rates and protect bioactive molecules is explored. Their structure enables sustained and targeted delivery of therapeutic agents, enhancing treatment efficacy; iii) 3D bioprinting: The use of IPN hydrogels as bioinks for 3D bioprinting is assessed, demonstrating their capability to construct intricate, biomimetic structures with high precision; and iv) Regenerative medicine: the development of biomimetic IPN hydrogels for regenerative medicine, emphasizing their potential to closely replicate natural biological environments, thereby promoting effective tissue repair and regeneration. IPN hydrogels emerge as a versatile and multifaceted platform with significant implications for advancing biomedical science and clinical therapies. Their diverse applications highlight their potential to revolutionize current biomedical practices and contribute to the development of innovative therapeutic solutions.

Selective chiral dimerization and folding driven by arene-perfluoroarene force.

Oligomerization and folding of chiral compounds afford diversified chiral molecular architectures with interesting chiroptical properties, but their rational and precise control remain poorly understood. In this work, we employed arene-perfluoroarene (AP) interaction to manipulate the folding and dimerization of alanine derivatives bearing pyrene and a perfluoronaphthalene derivative. Based on X-ray crystallography and nuclear magnetic resonance, the compound with a smaller tether and high skeleton rigidity self-assembled into double helical dimers by duplex hydrogen bonding and AP forces in a less polar solvent. Reversible disassociation occurred upon switching to a dipolar solvent or applying heating-cooling cycles. In comparison, the compound with increased skeleton flexibility folds into chiral molecular clamps in a less polar solvent, and is transformed into planar dimers upon switching to a polar solvent. The dynamic geometrical transformation between dimerization and folding was accompanied by chiroptical switching. Beyond the molecular and supramolecular level, we showed hierarchy control in the self-assembled nanoarchitectures and columnar and lamellar arrangements of their molecular packing. This work utilized AP forces to prepare and manipulate the chiral architectures at different hierarchical levels, enriching methodologies in precise chiral synthetic chemistry.

Strategies for arene dissociation from transition metal η6-arene complexes.

Transition metal η6-arene complexes have unique properties that facilitate a variety of arene substitution reactions, rendering π-activation a powerful approach for arene functionalization. For decades, these complexes have been studied in the context of coordination chemistry and synthetic methodology via stoichiometric reactivity; one central challenge in expanding the utility of arene functionalization via transition-metal-π-activation is the dissociation of the arene product that remains bound to the transition metal. In this perspective, we highlight representative strategies and methods for the removal and/or exchange of arenes from such complexes. Recent studies that implement these strategies toward catalytic processes are discussed, along with remaining challenges in this area.

PYTHON PROGRAM FOR STRUCTURE AND MOLECULAR FORMULA OF ORGANIC COMPOUNDS: AN ALTERNATIVE THEORETICAL APPROACH

A chemist always desires the automation of a chemistry laboratory as it reduces their workload by minimizing the time, energy, and effort. Automation when coupled with artificial intelligence is the need in today’s era as it gives more flexibility and power to the chemist. Using computer programs for a large number of such operations would further ease out these tasks as the desired objectives may be achieved with the click of a mouse. In this research article, the Python program has been used to arrive at the empirical and molecular formulas of some compounds. Python code has also been written for some compounds and their 2D and 3D structures drawn henceforth. With the introduction of Python language at the undergraduate level, this paper would allow chemistry students to make use of Python in organic chemistry as well. As a result, the chemistry students will be able to use the Python program for verification and classroom-based structure determination of various organic compounds. The students thus gain access and become equipped with computational skills that will be helpful in the modern world and can utilize the Python code as an alternative theoretical approach for these operations. While Python provides valuable computational tools, integrating thematic chemistry and new methodologies is crucial for advancing structural analysis. This approach enhances the depth and applicability of theoretical models beyond basic calculations. This paper is a perfect amalgamation of computer programming with organic chemistry and is an attempt to equip the chemists to do the above-mentioned operations theoretically from their desks.

Ammonia blends for gas-turbines: Preliminary test and CFD-CRN modelling

Ammonia, with its high hydrogen density, cost effectiveness and ease of storage, is emerging as a potential fuel to meet decarbonisation targets in various sectors. However, the combustion of pure ammonia poses challenges due to its low calorific value and reactivity. Existing gas turbine combustion systems, refined over decades with conventional fuels, struggle to maintain comparable flue gas emission concentrations when burning ammonia.Overcoming this challenge requires a paradigm shift in gas turbine combustion, with implications for chemistry and combustor design methodology. Research into the chemical kinetic mechanism for ammonia combustion worldwide reveals differences in flame radical pools compared to methane combustion. The dominant NOx formation mechanism shifts to a "fuel-bound nitrogen" type, which differs from the "thermal" mechanism in hydrocarbon and hydrogen combustion.The integration of chemical reaction rates into Computational Fluid Dynamics (CFD) models with transport equations results in impractical computational times for industrial applications. Therefore, streamlined approaches such as chemical reaction reduction methods are being developed. This involves the construction of lumped parameter models (Chemical Reactor Network - CRN) to identify key parameters that affect species production. A reliable CRN implementation involves the identification of critical volumes using a preliminary CFD approach (CFD-CRN).This paper analyses preliminary experimental tests by Baker Hughes on an industrial burner using different ammonia, hydrogen, and methane fuel mixtures. The NOx results show a different behavior between methane and hydrogen. While the detailed mechanisms behind these results remain open questions, the CFD-CRN modelling approach is adopted. A proposed CRN is investigated for methane, hydrogen, and ammonia blends, providing insight into key parameters and a reasonable physical explanation for the different NOx results. A single calibration parameter (ϕpilot/ϕref) is used to predict accurately NOx emissions using experimental data available from experimental campaign.

Versatility of threose nucleic acids: synthesis, properties, and applications in chemical biology and biomedical advancements.

This feature article delves into the realm of α-L-threose nucleic acid (TNA), an artificial nucleic acid analog characterized by a backbone comprising an unconventional four-carbon sugar, α-L-threose, with phosphodiester linkages connecting at the 2' and 3' vicinal positions of the sugar ring. Within this article, we encapsulate the potential, progress, current state of the art, and persisting challenges within TNA research. Kicking off with a historical overview of xeno nucleic acids (XNAs), the discussion transitions to the compelling attributes and structure-property relationships of TNAs as advanced tools when contrasted with natural nucleic acids. Noteworthy aspects such as their advantageous spatial arrangements of functional groups around the sugar ring, stable Watson-Crick base pairing, high binding affinity, biostability, biocompatibility, and in vivo bio-safety are highlighted. Moreover, the narrative unfolds the latest advancements in chemical and biological methodologies for TNA synthesis, spanning from monomer and oligomer synthesis to polymerization, alongside cutting-edge developments in enzyme engineering aimed at bolstering large-scale TNA synthesis for in vitro selection initiatives. The article sheds light on the evolution of TNA aptamers over time, expounding on the tools and selection techniques engineered to unearth superior binding aptamers and TNA catalysts. Furthermore, the article accentuates the recent applications of TNAs across diverse domains such as molecular detection, immunotherapy, gene therapy, synthetic biology, and molecular computing. In conclusion, we summarize the key aspects of recent TNA research, address persisting gaps and challenges, and provide crucial insights and future perspectives in the dynamic domain of TNA research.

Microscopy in food analysis: a review

Abstract The concern with food safety has been discussed since the end of the 19th century to improve its quality. The microscopic identification of plant species used in natura or in the elaboration of food products is extremely relevant because it is fast and satisfactory to characterize food composition and plant contaminants. Microscopy also allows evaluations of the hygienic quality of food, making it possible to observe physical, biological, and microbiological contaminants. Although it is an low-cost method compared to other chemical or molecular methodology, it relies on the expertise and knowledge of the analyst, especially in plant anatomy since it is a method that is mainly based on the identification of food products by comparison. Thus, this review aims to summarize studies related to the use of plant anatomy and the future perspectives of associations with other analytical methods for plant quality control. Therefore, this review is structured in two main topics: plant anatomy under light microscopy in food analysis and plant anatomy under microscopy associated with other methods.