Fertilizer Research Articles

Sustainable Paddy Farming in Edirne: Evaluating the Impacts of Excessive Fertilizer and Pesticide Use

This study examines the environmental and economic impacts of chemical fertilizer and pesticide use in paddy fields in Edirne Province, Türkiye. Chemical fertilizers, especially nitrogen and phosphorus, are crucial for boosting paddy yield and ensuring food security. However, recent trends indicate significant potassium deficiency and excessive pesticide use, leading to environmental degradation and higher production costs. Incorrect fertilization techniques and pesticide usage can lower product quality and yield, adversely affecting farmers’ incomes and the national economy. Surveying 139 paddy farmers, the research found that farmers apply the herbicidal active ingredients Tefuryltrione, Clomazone, Tembotrione, and Quinclorac at rates of 129.35%, 34.27%, 18.06%, and 155.29% above the recommended levels, respectively. The excessive use of pesticides costs each farmer an additional USD 925.67, totaling USD 5.32 million for Edirne. The majority of farmers (69.2%) do not conduct soil analysis, indicating a potential gap in adopting the best practices for soil management and fertilization. Farmers use 84% less potassium than recommended while applying nitrogen and phosphorus 13% and 14% above the recommended levels, respectively. The extra cost of fertilizer use in Edirne is calculated as USD 833,135.49. This situation harms the economy and may have adverse effects on the environment and potential health effects on consumers.

Optimizing Organic Fertilization for Marguerite Daisy (Argyranthemum frutescens): Impact of Application Rate and Frequency on Growth and Yield

Edible flowers are a new market for horticulture plants. Beyond their attractive shapes and colors, this group of plants contains secondary metabolites that benefit human health. This study aims to investigate the growth and flower yield of marguerite daisies using organic fertilizer at different rates and frequencies. The experiment was designed as a factorial, completely randomized design with two factors: fertilizer rate (0.5, 1.0, and 1.5 times compared to the total nitrogen content in chemical fertilizer) and frequency of organic fertilizer application (every 30 and 15 days). Slow-release chemical fertilizer (Osmocote 13-13-13) was used as a control. The experiment reveals that the rate of organic fertilizer application significantly affected the growth and flower yield of Marguerite daisy. Still, the frequency of organic fertilizer application did not significantly affect it. Application at 1.5 times yielded the most significant growth and flower production compared to 0.5 and 1 times of application. When comparing the results of organic fertilizer application with 13-13-13 chemical fertilizer, it was found that applying organic fertilizer 1.0 times and 1.5 times every 15 or 30 days resulted in similar plant growth and flower size as with the chemical fertilizer (p>0.05). However, chemical fertilizer produced the highest chlorophyll index (SPAD), accumulated flower buds, and flower blooming per pot (p<0.05). The plant requires more than 1.5 times the organic fertilizer application to achieve flower production equivalent to chemical fertilizer.

Effect of Vermicompost and Vermicompost Tea on Vegetative Growth of Rubber Plants under Nurseries Conditions

Success in rubber growing is linked to the production of vigorous plants in the nursery. However, this cruel stage faces difficulties related to the cost of chemical fertilizer and its availability. This work carried out in 4 localities (Bimbresso, Alépé, Abengourou and Daoukro) in Côte d’Ivoire aimed to improve the production of rubber plant material in nurseries in polyethylene bags using vermicompost and vermicompost tea. The experimental design used is a Fisher block with three treatments and two repetitions. The vermicompost, vermicompost tea and control factors were compared with each other. The addition of vermicompost tea and the control for three months while the addition of vermicompost was done only once. The results indicated that vermicompost tea is the most effective treatment for the vegetative development of plants regardless of the study site. In Bimbresso, the vermicompost tea and vermicompost treatments showed the highest increases with 1.23 and 1.21 mm.month-1, compared to 1.13 mm.month-1 for the control and the highest average values of diameter at the collar. 10.1 mm was obtained with vermicompost tea. Also, the highest rate of graftable plants, grafting success and plants transferable to the field was obtained with vermicompost tea. Likewise, the mortality rate obtained with vermicompost and vermicompost tea was less than 20%. These treatments could be recommended in the production of rubber plant material.

ASSISTANCE AND TRAINING IN THE MAKING OF BIOFERTILIZER “UNAIR” WITH LECTURE AND PRACTICE METHODS FOR FARMERS IN KEDUNGPRING VILLAGE, LAMONGAN, TO SUPPORT SUSTAINABLE AGRICULTURE

Many farmers use chemical fertilizers to increase their agricultural productivity, including farmers in Kedungpring Village, Kedungpring Subdistrict, Lamongan Regency. However, it is currently difficult for farmers to meet their fertilizer needs due to the policy of limiting fertilizer subsidies, therefore farmers must have the independence to meet their fertilizer needs. Biofertilizers can be an alternative because they are easy to make by themselves, and are more environmentally friendly than chemical fertilizers. Assistance and training on biofertilizer production for farmers in Kedungpring Village, Lamongan is needed. The purpose of this community service activity is to increase the knowledge of farmers in Kedungpring Village, Lamongan about biological fertilizers, and have the ability to make biological fertilizers, so that they can meet their fertilizer needs. This activity begins with the coordination and planning stages of the activity, providing material on biofertilizers to increase farmers' knowledge of biofertilizers using the lecture method, assistance and training in making biofertilizers using the lecture and practice methods so that farmers have the ability to make biofertilizers, and monitoring and evaluation carried out by the follow-up method. Based on the community service activities carried out, farmers' knowledge about biofertilizers has increased, which is indicated by most farmers having a higher post-test score than the pre-test score, also farmers are able to make their own biofertilizers which are indicated by the color of cloudy brown fertilizer and the distinctive sour smell of fermentation. Through this Community Service activity, farmers in Kedungpring Village also received six bioreactors for making biological fertilizers. Through this whole series of activities, it is an effort to realize sustainable agriculture, and it is hoped that farmers in Kedungpring Village, Lamongan can meet their fertilizer needs independently.

Biological Nitrogen Fixation for Sustainable Agriculture Development Under Climate Change–New Insights From a Meta‐Analysis

ABSTRACTBiological nitrogen fixation (BNF) in legume crops is a crucial ecosystem service that enhances soil nitrogen and reduces the need for chemical fertilisers. This study evaluates the factors influencing the proportion of plant nitrogen derived from atmospheric fixation (Ndfa) in legume crops. We compiled a global dataset spanning from 1980 to 2018 and used the 15N method to assess the impacts of crop species, climatic conditions, stand composition and nitrogen fertilisation on Ndfa. The global meta‐analysis reveals that the percentage of nitrogen derived from atmospheric fixation (Ndfa) in legumes ranges from 5% to 99%, with an average of 68%. Fodder legumes exhibited higher Ndfa, averaging 75%, while grain legumes showed more variability, ranging from 38% to 85%, depending on species and climatic conditions. The significant variability in Ndfa underscores the complexity of the process, which is influenced by species‐specific traits, ecological conditions and competition in mixed stands. However, the current data is insufficient for precise Ndfa estimation in nitrogen balances and decision support tools. The study highlights the need for further research on the impact of nitrogen fertilisation and stand composition on Ndfa. These findings emphasise the potential of BNF to support sustainable agriculture by improving nitrogen availability and reducing dependence on synthetic fertilisers and particularly susceptibility to climate change challenges. To optimise the benefits of BNF, future research should focus on refining fertilisation regimes and exploring species‐specific responses to various ecological conditions. Exploring adaptive strategies, like selecting drought‐tolerant legumes and optimising irrigation, is essential. This will enhance the application of BNF in diverse agricultural systems, contributing to more sustainable and efficient farming practices.

Evaluation of different breeding waste compost applications on lettuce cultivation: growth, quality, mineral elements, and heavy metals accumulation

In China, increasing breeding waste has caused environmental problems. This study explored the possibility of using breeding waste compost (BWC) instead of chemical fertilizer in vegetable cultivation. The experiment included no fertilizer (CK), 100% chemical fertilizer (CF), 10, 20, and 30% substitution of cow dung compost (CDC), goose dung compost (GDC), and duck dung compost (DDC) with chemical fertilizer (C01, C02, C03, G01, G02, G03, D01, D02, D03). The results showed that BWC, particularly GDC, promoted lettuce growth and development. Compared to CK, the leaf fresh and dry weight of G03 were the highest, increasing significantly by 6.60 and 7.29 times, and the root fresh and dry weight of G02 were the highest, increasing significantly by 12.72 and 6.00 times. Different BWC improved soluble sugar, soluble protein, and Vitamin C to varying degrees, and the nitrate contents of some BWC treatments were lower than that of CK and CF. Conversely, CF had the highest nitrate accumulation and limited effects on certain growth and quality parameters. The mineral elements in lettuce were also affected by the type and dosage of fertilizers. The total nitrogen of CF, total phosphorus of G03, total potassium of G02, Ca and Mg of D01, Fe of CF, and Zn of G02 were at the peak. The rapid increase of biomass in GDC treatments led to reductions in Ca and Fe. Applying fertilizers would affect heavy metals in lettuce to unequal degrees, but all were within the food safety scope. The principal component analysis revealed the comprehensive effect of GDC treatments was recommended.

Wood biochar induced metal tolerance in Maize (Zea mays L.) plants under heavy metal stress

Due to metal toxicity, widespread industrialization has negatively impacted crop yield and soil quality. The current study was aimed to prepare and characterize biochar made from wood shavings of Pinus roxburghii and to determine the plant growth promoting and heavy metal detoxification of cadmium (Cd) and chromium (Cr) contaminated soil. FTIR SEM coupled with EDX characterization of biochar was performed; Cd and Cr were used at a rate of 20 mg/kg. Biochar was used at the rate of 50 mg/kg for various treatments. The completely randomized design (CRD) was used for the experiment and three replicates of each treatment were made. Various agronomic and enzymatic parameters were determined. The results indicated that all growth and enzymatic parameters were enhanced by the prepared biochar treatments. The most prominent results were observed in treatment T5 (in which shoot length, root length, peroxidase dismutase (POD), superoxide dismutase (SOD) catalyzes (CAT), and chlorophyll a and b increased by 28%, 23%, 40%, 41%, 42%, and 27%, respectively, compared to the control). This study demonstrated that biochar is a sustainable and cost-effective approach for the remediation of heavy metals, and plays a role in plant growth promotion. Farmers may benefit from the current findings, as prepared biochar is easier to deliver and more affordable than chemical fertilizers. Future research could clarify how to use biochar optimally, applying the minimum amount necessary while maximizing its benefits and increasing yield.

The Comparative Effects of Phosphorus-Enriched Organomineral Fertilization on Some Chemical Properties of Calcareous Soils

ABSTRACT Phosphorus is a very important plant nutrient for the development of plants and its production is not unlimited in the world. In addition, excessive use can lead to soil and water pollution. In this respect, phosphorus should be used effectively in plant production, especially in calcareous soils. The aim of this study was to comparatively determine the effects of phosphorus-enriched organomineral fertilization (p-OMF), chemical phosphorus fertilization (CF), and organic fertilization (OF) on some chemical properties of a calcareous soil. The experiment was established in Söke county of Aydın province in the Southwest of Türkiye. In this study, four doses of dairy manure (DM1: 10, DM2: 20, DM3:30, and DM4: 40 t ha−1) in OF, four phosphorus doses (P1: 10, P2: 20, P3:30, and P4: 40 kg P ha−1) in the CF system, and sixteen different doses of OMF formed by the combinations of these two fertilizers were applied excluding control. According to the main research findings, p-OMFs increased the soil organic matter (SOM), salinity, total nitrogen (N), available phosphorus (P), and potassium (K) by 60.1%, 20.9%, 22.1%, 140%, and 17.2%, respectively. They also decreased soil pH by 4%. As a result, it was determined that the most effective organomineral fertilizer combination that maintains soil properties at appropriate levels in calcareous soils is DM3+P2 (30 t ha−1 dairy manure +20 kg P ha−1).

Integrated assessment for groundwater quality and flood vulnerability in coal mining regions.

Coal mining activities greatly damage water resources, explicitly concerning water quality. The adverse effects of coal mining and potential routes for contaminants to migrate, either through surface water or infiltration, into the groundwater table. Dealing with pollution from coal mining operations is a significant surface water contamination concern. Consequently, surface water resources get contaminated, harming nearby agricultural areas, drinking water sources, and aquatic habitats. Moreover, the percolation process connected with coal mining could alter groundwater quality. Subsurface water sources can get contaminated by toxins generated during mining activities that infiltrate the soil and reach the groundwater table. The aims of this study are the creation of models and the provision of proposals for corrective measures. Twenty-five scenarios were simulated using MODFLOW; according to the percolation percentage and contamination, 35% of the study area, i.e., the middle of the research area, was the most affected. About 38.08% of the area around the mining zones surrounding Margherita is prone to floods. Agricultural areas, known for applying chemical fertilizers, are particularly vulnerable, generating a risk of pollution to surrounding water bodies during flooding. The outputs of this research contribute to identifying and assessing flood-vulnerable regions, enabling focused measures for flood risk reduction, and strengthening water resource management.

Long-Term Manuring Enhanced Compositional Stability of Glomalin-Related Soil Proteins through Arbuscular Mycorrhizal Fungi Regulation

Glomalin-related soil proteins (GRSP) play a crucial role in strengthening soil structure and increasing carbon (C) storage. However, the chemical stability of GRSP and related arbuscular mycorrhizal fungi (AMF) community response to fertilization remains unclear. This study investigated C and nitrogen (N) contents, three-dimensional fluorescence characteristics in GRSP, and AMF properties based on a field experiment that was subjected to 29 years of various fertilizations. The experiment included treatments with no fertilizer (CK), chemical fertilizer (NPK), manure (M), and manure combined with NPK (NPKM) treatments. Results showed that GRSP contained 37–49% C and 6–9% N, respectively. Compared with CK and NPK, the C and N proportions in GRSP significantly increased under M and NPKM. Using the parallel factor model, four fluorescent components of GRSP were identified: one fulvic acid-like component (C2), one tyrosine-like component (C4), and two humic acid-like components (C1, C3). Under M and NPKM, the fluorescent intensity of C2 and C4 decreased, while the humification index (HIX) increased relative to CK and NPK, indicating that organic fertilization could enhance the stability of GRSP. The C and N proportion in GRSP positively associated with soil organic C (SOC), total N (TN), available phosphorus (AP), AMF biomass, and diversity, while C2 and C4 showed negative associations. Structural equation modeling further revealed that manure-induced changes in pH, SOC, TN, and AP increased AMF biomass and diversity, thereby altering GRSP composition and stability. This study provides valuable insights into the compositional traits of GRSP, contributing to sustainable soil management and C sequestration in agroecosystems.

Effect of Integrated Nutrient Management (INM) on Soil Physico-chemical Properties in Wheat (Triticum aestivum L.) Intercrop under Beul (Grewia optiva Drummond.) Based Agroforestry System and Open Condition

The raising human health hazards due to excessive use of chemical fertilizers and pesticides in India leads cancer patients. To overcome such problems there is need of Integrated Nutrient Management (INM) in our major crop wheat and further to improve soil fertility under agroforestry system. Therefore, the present investigation was carried out to study the impact of INM on the physical and chemical properties of soil when wheat intercropped under Beul (Grewia optiva) based agroforestry system in the mid-hill region of Himachal Pradesh at Dr. Y. S. Parmar University of Horticulture and Forestry, Nauni, Solan, HP (India). INM practices were applied to evaluate their effectiveness in enhancing soil fertility, crop yield, and overall soil health. The experimental setup included various treatment combinations of organic and inorganic fertilizers viz., T1: RDF, T2: FYM, T3: Vermicompost, T4: Goat manure, T5: 50% RDF+50% FYM, T6: 50% RDF+50% VC, T7: 50% RDF+50% GM, and T8: Control to determine their synergistic effects on the soil and cereal crop. These treatments were randomly assigned to plots under two planting conditions: S1 (under Beul based agroforestry system) and S2 (open condition), with three replications each, following in a factorial randomized block design (RBD). The key physical parameters such as bulk density (g/cm3), particle density (g/cm3), porosity (%), and soil moisture (%) were measured along with chemical properties of soil including soil pH, EC (dS/m1), organic carbon content (%), and nutrient availability of N, P and K in kg per hectare. The soil data of two consecutive years and pooled data were analyzed in R Statistical Software. The results demonstrated that INM significantly improved both the physical and chemical soil properties in agroforestry system as compared to control treatment i.e. open condition. The use of 100 % FYM found to be best treatment during the course of study among various treatments of INM for soil porosity, soil moisture, soil pH, available N, P and K. The soil structure and nutrient availability were increased in second year as compared to first year which will lead to improve the growth and yield of wheat crop. The study underscores the importance of adopting INM practices in agroforestry system to achieve sustainable agriculture as well as chemical free agriculture in hilly regions of Himachal Pradesh which will be helpful for human health. Thus, the use 100% FYM fertilizer is recommended for farmers in hilly area of Himachal Pradesh for sustainable agriculture and natural farming. The findings provide valuable insights for farmers and policymakers aiming to optimize crop production and maintain soil health in similar agro-ecological zones.

Effect of Chinese Milk Vetch on Zinc Content and Zinc Absorption of Rice in Purple Tidal Mud Soil

Rice is a staple food crop that feeds billions globally. Addressing Zn deficiency in rice is crucial for improving nutrition and food security. Zn deficiency in rice is a widespread issue, especially in purple tidal mud substrates, which often exhibit low Zn availability. The objective of this two-year pot study was to explore the relationship between Zn content, yield components, and Zn absorption in rice grown in purple tidal mud substrate with varying amounts of Chinese milk vetch (Astragalus sinicus L.) incorporation. The experimental design consisted of seven treatments: an unfertilized control, a Chinese milk vetch control, a chemical fertilizer control, and four treatment variations incorporating Chinese milk vetch alongside chemical fertilizer applications. The results indicated that planting and applying Chinese milk vetch improved the grain yield of rice in purple tidal mud substrate, and the yield increased with higher levels of Chinese milk vetch applied. The increased grain yield resulted in higher Zn absorption in rice grains. The application of Chinese milk vetch, both solely and in combination with chemical fertilizers, had varying effects on zinc uptake and grain zinc formation efficiency in early and late rice, with the control and low-level Chinese milk vetch treatments generally exhibiting the highest performance across the two-year period. By introducing Chinese milkvetch following the use of chemical fertilizers, the Zn content in rice grains increased starting from the second year. The treatment with Chinese milkvetch applied at a rate of 2.25 t/hm2 showed the best results in increasing the Zn content in rice grains. The increase in Zn content and Zn uptake by the rice plants gave rise to a lowering of the DTPA-extractable Zn content in the purple tidal mud substrate. Sole Chinese milk vetch application and using Chinese milk vetch following chemical fertilizer application both increased Zn content extracted by DTPA in purple tidal mud substrate.

Multi-evidences investigation into spatiotemporal variety, sources tracing, and health risk assessment of surface water nitrogen contamination in China

A comprehensive understanding of nitrogen pollution status, especially the identification of sources and fate of nitrate is essential for effective water quality management at the local scale. However, the nitrogen contamination of surface water across China was poorly understood at the national scale. A dataset related to nitrogen was established based on 111 pieces of literature from 2000 to 2020 in this study. The spatiotemporal variability, source tracing, health risk assessment, and drivers of China's surface water nitrogen pollution were analyzed by integrating multiple methods. These results revealed a significant spatiotemporal heterogeneity in the nitrogen concentration of surface water across China. Spatially, the Haihe River Basin and Yellow River Basin were the basins where surface water was seriously contaminated by nitrogen in China, while the surface water of Southwest Basin was less affected. Temporally, significant differences were observed in the nitrogen content of surface water in the Songhua and Liaohe River Basin, Pearl River Basin, Southeast Basin, and Yellow River Basin. There were 1%, 1%, 12%, and 46% probability exceeding the unacceptable risk level (HI＞1) for children in the Songhua and Liaohe River Basin, Pearl River Basin, Haihe River Basin, and Yellow River Basin, respectively. The primary sources of surface water nitrate in China were found to be domestic sewage and manure (37.7%), soil nitrogen (31.7%), and chemical fertilizer (26.9%), with a limited contribution from atmospheric precipitation (3.7%). Human activities determined the current spatiotemporal distribution of nitrogen contamination in China as well as the future development trend. This research could provide scientifically reasonable recommendations for the containment of surface water nitrogen contamination in China and even globally.

Integrated Manure and Fertilizer Application: A Pathway to Enhanced Rice Yield and Soil Health

A research study was carried out in the Soil Science field laboratory of Bangladesh Agricultural University, Mymensingh during the Aman season of 2021 to examine the effect of various manure and fertilizer combinations for optimizing the growth and yield of rice. The experiment employed a Randomized Complete Block Design (RCBD) with 3 replications across eight treatments. The treatment combinations were: T­1 (Control), T2 (100% Recommended Fertilizer Dose (RFD)), T3 (75% RFD + Cowdung (CD) @ 5 t ha-1), T4 (75% RFD + Poultry manure (PM) @ 3 t ha-1), T5 (75% RFD + Compost @ 5 t ha-1), T6 (75% RFD + Dhaincha Green Manure (GM) @ 10 t ha-1), T7 (50% RFD +CD @ 2.5 t ha-1 + PM @ 1.5 t ha-1) and T8 (50% RFD + Compost @ 2.5 t ha­-1 + Dhaincha GM 5 t ha-1). Statistical analysis conducted using R programming revealed that T6 markedly improved growth metrics and yield-related traits, leading to a 12.2% rise in grain yield and a 13.24% rise in straw yield compared to T2, where only chemical fertilizers were applied. This treatment also achieved the greatest macronutrient content and uptake, outperforming other treatments (T3, T4, and T5) with equal nutrient amounts. Therefore, application of 75% recommended fertilizer dose along with dhaincha green manure (10 t ha-1) offers a promising strategy for successfully cultivating BRRI dhan71 with a good soil health. This approach offers farmers a cost-effective method to boost productivity and support long-term soil fertility, and policymakers could promote it to enhance agricultural sustainability.

Pendampingan Revitalisasi Bumdes Amanah Mandiri Desa Jatimukti, Kabupaten Sumedang, Provinsi Jawa Barat

This article discusses the revitalization of the Village-Owned Enterprises (BUMDes) Amanah Mandiri in Jatimukti Village, Jatinangor Subdistrict, Sumedang Regency. The aim of this community service was to reactivate the previously inactive BUMDes through a series of activities held from June 3 to 6, 2024, including participatory FGDs, education on BUMDes and village potential mapping, and simulation and assistance in organic fertilizer production. Methods employed included FGDs, training sessions, simulations, and participatory dialogues with various stakeholders. This community service was collaboratively conducted by involving agricultural advisors from Sumedang Regency. The main findings indicate that integrated participatory approaches and education effectively stimulate interest and economic activities within BUMDes, particularly through innovative organic fertilizer products. The conclusion drawn from this study is that with the right approach, BUMDes can serve as the backbone of village economies, enhance community welfare, and reduce farmers' dependency on chemical fertilizers.

Utilization of Quality Organic Fertilizer at P4S Sari Sedana, Badung, Bali

This Community Partnership Program (CSP) conducted in Desa Selat, Kecamatan Abiansemal, Kabupaten Badung, has achieved significant advancements in organic agricultural development. The program focused on the implementation of both hard and soft technologies. Hard technologies included the development of high-quality organic fertilizers using local waste materials such as livestock manure, biochar, Trichoderma, NPK fertilizers, and dolomite, processed with simple equipment. These innovations addressed local waste management issues and enhanced agricultural productivity. Soft technologies involved comprehensive training on production management, business operations, and marketing strategies, which empowered the P4S Sari Sedana Group with essential skills for sustainable operation. The program demonstrated high relevance to local needs, effectively transforming waste into valuable resources and introducing environmentally friendly production methods. It also fostered active community participation and generated substantial environmental and economic benefits, including increased soil fertility and reduced reliance on chemical fertilizers. The project produced several key outputs: high-quality organic fertilizer, enhanced community skills, scientific publications, intellectual property registration, and online media coverage. Future sustainability will be supported through institutional capacity building, market expansion, product innovation, and ongoing monitoring and evaluation.

GROUNDWATER QUALITY INFLUENCING THE POTATO TUBER YIELD IN DOABA REGION OF PUNJAB, INDIA

Groundwater as a source of irrigation is extensively practiced in the Doaba region of Punjab, alongside high inputs such as chemical fertilizers and pesticides. These inputs pose dual threats to the deterioration and deepening of groundwater. Therefore, a survey-based investigation was conducted in the region, involving the collection of groundwater samples, their physico-chemical analysis, and the assessment of harvested potato tuber yields. The data analysis revealed that farmers are extracting groundwater from borewells at a depth of 254.0 feet on average, with a delivery depth of 145.67 feet. The analytical results indicated that all the collected groundwater samples were suitable for irrigation purposes. Furthermore, there was a significantly positive correlation between tuber yield and nitrate content of groundwater. Thus, it is crucial to consider the contribution of nitrate from groundwater when recommending nitrogen usage.

RICE HUSK ASH (RHA) - A POTENTIAL SOURCE FOR IMPROVING PHOSPHORUS NUTRITION IN POTATO (S. tuberosum L.)

Use of alternative sources of nutrients such organic manures, residues and by-products to chemical fertilizers may enhance not only the yield but cut down the fertilizers use. Therefore, present investigation was carried out to explore the possibility of using rice husk ash (RHA) as a source of phosphorus in potato crop. Two pot experiments were conducted on Kufri Jyoti and Kufri Girdhari to determine the effect of rice husk ash on phosphorus nutrition of the crop during 2020-21. Application of rice husk ash (RHA) significantly increased the plant height, haulms (leaf plus stem) biomass, root weight, chlorophyll content (SPAD value) and P content. In Kufri Jyoti, application of 20g RHA per pot significantly increased haulms biomass, tuber yield, relative leaf chlorophyll content and P content in haulms by 17.8 per cent over control. In Kufri Girdhari, application of 40g RHA/ pot also increased the P content by (14.6 %) in haulms over control besides significant increase in haulm yield, root mass and relative leaf chlorophyll content. It was observed that subjecting the crop to moisture stress by withholding the irrigation towards harvest, senescence was delayed in plants fertilized with rice husk ash. RHA application showed a significant and positive correlation with root length and tuber yield (r=0.70). The results of the present study revealed that RHA can be used as a source of phosphorus for potato crop and has potential to improve water relations and nitrogen nutrition as indicated by improved relative leaf chlorophyll content in plants fertilized with RHA.

Tissue Culture in Banana Cultivation: A Review of its Impact on Disease Management, Yield Improvement, and Sustainable Production

In several tropical and subtropical areas, bananas (Musa spp.) are an important food source and commercial crop. Disease transmission and production uncertainty are common problems with traditional propagation techniques such as suckers. By supplying consistent, high-quality, and disease-free planting material, tissue culture, known as micro propagation, represents a potential alternative. The application of tissue culture has greatly enhanced disease management in banana cultivation. Reducing the incidence of ailments like Fusarium wilt, bacterial wilt, and Banana Bunchy Top Virus (BBTV), pathogen-free plantlets are grown in controlled conditions. This strategy lessens the demand for artificial fertilizers while also promoting healthier crops and lessening the impact on the environment. Micropropagated banana plants demonstrated consistent growth, early fruiting, and increased yield in contrast to conventionally propagated plants. Tissue-cultured plants exhibit strong development and genetic uniformity, which contribute to these advantages and more consistent and dependable harvests. By promoting efficient land use and reducing the need for chemical inputs, tissue culture enhances sustainability in the production of bananas. The growing demand for bananas may be met without requiring the increase of agricultural land thanks to tissue culture techniques, which enable the large-scale production of enhanced banana cultivars at a quick rate. Furthermore, using planting material free of disease lowers input costs and crop losses, supporting more environmentally friendly farming methods.

Distribution of tetracycline resistance genes within an organic fertilizer-amended soil–rice continuum

The transmission of antibiotic resistance genes (ARGs) to humans through the consumption of plants grown in manure-amended soils is a critical concern. However, the effect of manure application on the profiles of tetracycline resistance genes (TRGs) within the soil–rice continuum remains unclear. In this study, tetracycline (TC) content, bacterial communities, abundance of 8 TRGs, and class 1 integron (intI1) were characterized using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), Illumina sequencing, and quantitative PCR (qPCR) in rhizosphere soils, roots, and grains of rice exposed to pig manure (PM), rapeseed cake (RC), and chemical fertilizer (CF), respectively. Our findings indicate that the type of sample was the primary determinant of TRGs abundance variation within the soil-rice continuum, with a consistent decline from rhizosphere soils to roots to grains. Furthermore, fertilization type significantly influenced TRGs abundance, with the highest levels observed in PM treatment. TetZ and tetX were predominant, constituting over 90% of total TRGs abundance across all samples. In addition, the mechanism of TRGs profile formation varies with sample types. Bacterial communities-TC content-intI1 interactions determined the change in TRGs abundance in rhizosphere soils, and bacterial communities constituted the most important factor affecting TRGs abundance within the roots. However, bacterial communities and/or intI1 poorly explained the change in TRGs abundance within the grains. Our study attempts to explore the underlying mechanism for the profiles of TRGs in soil–rice continuums exposed to manure, as well as provides a theoretical basis for controlling the spread of endogenous antibiotic resistance within rice grown in soil receiving pig manure.