Chemical Extraction Research Articles

Malaria: biochemical, physiological, diagnostic, and therapeutic updates.

Malaria has been appraised as a significant vector-borne parasitic disease with grave morbidity and high-rate mortality. Several challenges have been confronting the efficient diagnosis and treatment of malaria. Google Scholar, PubMed, Web of Science, and the Egyptian Knowledge Bank (EKB) were all used to gather articles. Diverse biochemical and physiological indices can mirror complicated malaria e.g., hypoglycemia, dyslipidemia, elevated renal and hepatic functions in addition to the lower antioxidant capacity that does not only destroy the parasite but also induces endothelial damage. Multiple trials have been conducted to improve recent points of care in malaria involving biosensors, lap on-chip, and microdevices technology. Regarding recent therapeutic trials, chemical falcipain inhibitors and plant extracts with anti-plasmodial activities are presented. Moreover, antimalaria nano-medicine and the emergence of nanocarrier (either active or passive) in drug transportation are promising. The combination therapeutic trials e.g., amodiaquine + artemether + lumefantrine are presented to safely counterbalance the emerging drug resistance in addition to the Tafenoquine as a new anti-relapse therapy. Recognizing the pathophysiology indices potentiate diagnosis of malaria. The new points of care can smartly manipulate the biochemical and hematological alterations for a more sensitive and specific diagnosis of malaria. Nano-medicine appeared promising. Chemical and plant extracts remain points of research.

Fluid–Solid Mixing Transfer Mechanism and Flow Patterns of the Double-Layered Impeller Stirring Tank by the CFD-DEM Method

The optimization design of the double-layered material tank is essential to improve the material mixing efficiency and quality in chemical engineering and lithium battery production. The draft tube structure and double-layered impellers affect the flow patterns of the fluid–solid transfer process, and its flow pattern recognition faces significant challenges. This paper presents a fluid–solid mixing transfer modeling method using the CFD-DEM coupling solution method to analyze flow pattern evolution regularities. A porous-based interphase coupling technology solved the interphase force and could be used to acquire accurate particle motion trajectories. The effect mechanism of fluid–solid transfer courses in the double-layered mixing tank with a draft tube can be obtained by analyzing key features, including velocity distribution, circulation flows, power, and particle characteristics. The research results illustrate that the draft tube structure creates two major circulations in the mixing transfer process and changes particle and vortex flow patterns. The circulating motion of the double-layered impellers strengthens the overall fluid circulation, enhances the overall mixing efficiency of the fluid medium, and reduces particle deposition. Numerical results can offer technical guidance for the chemical extraction course and lithium battery slurry mixing.

Biogenic potassium: sources, method of recovery, and sustainability assessment

Abstract Nutrient management methods based on ecosystems are crucial for providing agricultural nutrient needs while reducing the environmental impact of fertilizer usage. With increasing agricultural production, the global demand for potassium is increasing, with India importing potassium from countries like Canada, USA, Israel, and Russia. Biomass-fired industries generate biomass ash as a residue so management of the resultant ash is important. Agricultural residue ashes contain abundant potassium so could potentially be used for fertilizer application. This review describes different potassium sources and recovery processes, including chemical precipitation, water extraction, solvent extraction, membrane separation, and ionic exchange. Extraction time, temperature, and solid to solvent ratio affect the recovery of potassium from biomass ash. Water extraction is the most commonly used method for potassium recovery from biomass ash. The environmental impact of potassium fertilizer recovered from biomass ash is less than that of mining source of potash. This paper discusses topics not covered in previous reviews, such as different biosources of potassium, latest recovery methods, and life cycle assessment of these methods. The gaps identified in the reports are addressed, and future research opportunities are presented.

Chemical characterization, multivariate analysis and comparison of biological activities of different parts of Fraxinus mandshurica.

Fraxinus mandshurica (Oleaceae) is used as a traditional medicinal plant for the treatment of red eyes, menstrual disorders, excessive leucorrhea, chronic bronchitis and psoriasis. To perform chemical characterization of the secondary metabolites of F. mandshurica roots, bark, stems and leaves, 32 samples were collected from eight provinces in this study. A total of 64 chemical components were detected from four different parts of F. mandshurica by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry. Meanwhile, a total of nine secoiridoids were obtained by natural product chemical extraction, isolation and identification methods. Quantitative analysis by high-performance liquid chromatography-diode array detection-mass spectrometry showed the highest total content of secoiridoids in the bark, which is also consistent with the traditional medicinal parts. The results of methodological validation showed that the correlation coefficient (R2) values were all >0.9993, indicating a good linear range of the standard curve, while the relative standard deviations of precision, reproducibility and stability were <3%, and the spiked recoveries ranged from 98.22 to 102.27%, indicating that the experimental method was reliable and stable. In addition, fingerprinting and a heatmap were established to demonstrate the content trends of F. mandshurica more visually from different origins. Multivariate analysis, including principal component analysis and partial least squares discriminant analysis, was performed to determine the chemical characteristics of different parts of F. mandshurica, and six characteristic secoiridoids that could be used to distinguish different origins were screened. Finally, the inhibition of tyrosinase, α-glucosidase, acetylcholinesterase and pancreatic lipase activities by the nine characteristic compounds and extracts from different parts were investigated, and the results showed that they all exhibited different degrees of enzyme activity inhibition and thus have potential applications in whitening and blemish removal, hypoglycemia, anti-Alzheimer's disease and anti-obesity as a new source of natural enzyme activity inhibitors. This study establishes an identification and evaluation method applicable to phytochemistry of different origins, which is a guideline for quality control, origin evaluation and clinical application of traditional medicinal plants. This is also an unprecedented study on the identification of the chemical composition of different parts of F. mandshurica, characteristic compounds and the inhibition of enzyme activity of extracts from different parts.

Assessment of the stabilization effect of ferrous sulfate for arsenic-contaminated soils based on chemical extraction methods and in vitro methods: Methodological differences and linkages

Stabilization of arsenic-contaminated soils with ferrous sulfate has been reported in many studies, but there are few stabilization effects assessments simultaneously combined chemical extraction methods and in vitro methods, and further explored the corresponding alternative relationships. In this study, ferrous sulfate was added at FeAs molar ratio of 0, 5, 10 and 20 to stabilize As in 10 As spiked soils. Stabilization effects were assessed by 6 chemical extraction methods (toxicity characteristic leaching procedures (TCLP), HCl, diethylenetriamine pentaacetic acid (DTPA), CaCl2, CH3COONH4, (NH4)2SO4), and 4 in vitro methods (physiologically based extraction test (PBET), in vitro gastrointestinal method (IVG), Solubility Bioaccessibility Research Consortium (SBRC) method, and the Unified Bioaccessibility Research Group of Europe method (UBM)). The results showed that the HCl method provides the most conservative assessment results in non-calcareous soils, and in alkaline calcareous soils, (NH4)2SO4 method provides a more conservative assessment. In vitro methods provided significantly higher As concentrations than chemical extraction methods. The components of the simulated digestion solution as well as the parameters may have contributed to this result. The small intestinal phase of PBET and SBRC method produced the highest and lowest ranges of As concentrations, and in the range of 127–462 mg/kg and 68–222 mg/kg when the FeAs molar ratio was 5. So the small intestinal phase of PBET method may provide the most conservative assessment results, while the same phase of SBRC may underestimate the human health risks of As in stabilized soil by 51 %(at a FeAs molar ratio of 5). Spearman correlation analysis indicated that the small intestinal phase of PBET method correlated best with HCl method (correlation coefficient: 0.71). This study provides ideas for the assessment of stabilization efforts to ensure that stabilization meets ecological needs while also being less harmful to humans.

Potential pitfalls when using popular chemical extractions to characterize Al‐ and Fe‐containing soil constituents

Potential pitfalls when using popular chemical extractions to characterize Al‐ and Fe‐containing soil constituents

Comparative study of chemical speciation and leaching feature of typical heavy metals in coarse and fine slags from the entrained-flow coal gasification

ABSTRACT The dissimilarity in chemical speciation and release properties of heavy metals will result in varying levels of environmental hazards and potential applications of coal gasification coarse and fine slags. The distribution, chemical speciation, leaching features, and potential environmental risks of heavy metals (Cr, Cu, Cd, Zn, Pb, and Mn) in coarse and fine slags from different coal gasification processes were revealed and compared by the sequential chemical extraction method, Horizontal vibration method, and pH-dependent leaching experiments. The results show that Cr (516.78 mg/kg) in coarse slag, Zn (348.50 mg/kg) and Pb (214.78 mg/kg) in fine slag are highly enriched and the content exceeds the screening value, indicating the need for better management during stockpiling and landfilling. The distribution of Cr, Cu, Pb, and Mn in residual state, Zn in Fe-Mn oxide bound state, and Cd in unstable bound state is the highest in both coarse slag and fine slag. The leaching toxicity of heavy metals (HMs) does not exceed the standard, but the leachate is classified as Level V surface water or groundwater quality due to the leaching concentration of Pb exceeding 0.1 mg/L. Furthermore, leaching of Cr, Zn, Cd, and Pb in fine slag is much more dependent on pH compared to coarse slag, and the concentrations of Zn and Pb exceed emission standards at pH < 3.0. The high concentration of heavy metals caught on the surface of fine slag is more harmful to the environment. The properties of HMs in the slags and pH of the application environment must be comprehensively considered before coal gasification slag disposal, especially fine slag.

Phosphorus speciation in manure and fertilizer impacted Mid-Atlantic coastal plain soils.

Historical applications of manures and fertilizers at rates exceeding crop P removal in the Mid-Atlantic region (United States) have resulted in decades of increased water quality degradation from P losses in agricultural runoff. As such, many growers in this region face restrictions on future P applications. An improved understanding of the fate, transformations, and availability of P is needed to manage P-enriched soils. We paired chemical extractions (i.e., Mehlich-3, water extractable P, and chemical fractionation) with nondestructive methods (i.e., x-ray absorption near edge structure [XANES] spectroscopy and x-ray fluorescence [XRF]) to investigate P dynamics in eight P-enriched Mid-Atlantic soils with various management histories. Chemical fractionation and XRF data were used to support XANES linear combination fits, allowing for identification of various Al, Ca, and Fe phosphates and P sorbed phases in soils amended with fertilizer, poultry litter, or dairy manure. Management history and P speciation were used to make qualitative comparisons between the eight legacy P soils; we also speculate about how P speciation may affect future management of these soils with and without additional P applications. With continued P applications, we expect an increase in semicrystalline Al and Fe-P, P sorbed to Al (hydro)oxides, and insoluble Ca-P species in these soils for all P sources. Under drawdown scenarios, we expect plant P uptake first from semicrystalline Al and Fe phosphates followed by P sorbed phases. Our results can help guide management decisions on coastal plain soils with a history of P application.

Acidithiobacillus ferrooxidans Leaching of Silica-Sulfide Gold Ores from May-Hibey Deposits, Tigray, Ethiopia

Oxidative leaching is an inexpensive alternative to using chemical cyanide extraction methods for gold from low-grade gold sulfide. Oxidation of finely ground gold-bearing ore by Acidithiobacillus ferrooxidans was evaluated in terms of cell density, pH, and leaching efficiency of Fe and Au in shake flask experiments. The compositional and elemental analyses of the beneficiated ore were analyzed using XRD and EDXRF spectroscopy. The ore’s primary constituents are gold (4.356 mg/L), silicon, iron, and sulfur (62.456, 15.441, and 7.912 wt%, respectively). XRD spectra, the main phases of the concentrated ore, showed that the major components of the ore were quartz, syn, silicon sulfide, pyrite, and polymetallic elements such as silderenrite, gismondine, siderenikite, hematite, and syn. The experimental results, with Acidithiobacillus ferrooxidans bacteria and blank, were evaluated. The pH of the blank remained nearly constant, and the pH of the bioleached was occasionally lowered. The A. ferrooxidans strain always grew better throughout the bioleaching process. For the A. ferrooxidans strain, the cell density of cells reached a maximum of 90.00 × 106 cells/mL after the 11th week and decreased to 87.00 × 106 cells/mL after the 12th week. The decrease in cell density may be due to the presence of polymetallic elements such as Al, Cr, Ti, and Ni, leading to reduced metal tolerance of the A. ferrooxidans strain. In the A. ferrooxidans leaching process, the maximum total iron and gold extraction reached 92.16% (14.23 mg/L) and 99.97% (4.355 ppm), respectively, after the 11th week, and leaching tends to decrease up to 14 weeks, which may be due to the formation of secondary minerals. More research will be performed to optimize the procedure and leaching kinetic, examine the impact of metal content, and take into account the potential for bioleaching process pollution in addition to the amount of gold recovered.

A Review of Recent Progresses on Olive Oil Chemical Profiling, Extraction Technology, Shelf-life, and Quality Control.

Olive oil (OO) is widely recognized as a main component in the Mediterranean diet owing to its unique chemical composition and associated health-promoting properties. This review aimed at providing readers with recent results on OO physicochemical profiling, extraction technology, and quality parameters specified by regulations to ensure authentic products for consumers. Recent research progress on OO adulteration were outlined through a bibliometric analysis mapping using Vosviewer software. As revealed by bibliometric analysis, richness in terms of fatty acids, pigments, polar phenolic compounds, tocopherols, squalene, sterols, and triterpenic compounds justify OO health-promoting properties and increasing demand on its global consumption. OO storage is a critical post-processing operation that must be optimized to avoid oxidation. Owing to its great commercial value on markets, OO is a target to adulteration with other vegetable oils. In this context, different chemometric tools were developed to deal with this problem. To conclude, increasing demand and consumption of OO on the global market is justified by its unique composition. Challenges such as oxidation and adulteration stand out as the main issues affecting the OO market.

Yield Optimization for The Extraction of Organic Compounds from Okra Leaves Wastes

An important area of research is the extraction of organic chemicals from plants and herbs. Considering the fact that the extracts have numerous commercial and pharmacological uses. Furthermore, limiting the optimal working region is made easier by employing an appropriate experimental design. Solvent extraction is the technique most frequently used to separate organic components from plants. However, the conditions of extracting solvent that is utilized greatly affects the yields of the extract and, as a result, the organic activities of the plant parts. This research deals with the extraction of solid organic compounds from the okra leaves using water as a solvent. A Soxhlet apparatus was used for the extraction process. The influence of extraction time, solvent volume, and okra powder mass on the yield percentage was optimized. Two mathematical models were suggested: second-order polynomials and power models. A higher correlation coefficient was obtained with the polynomial model. The maximum extraction yield was obtained at optimum values of 200.3 min, 29.07 g, and 290.7 ml for time, okra powder mass, and solvent volume, respectively. It is evident from mathematical formulas that the impact of time was less significant than the effects of solvent volume and powder mass. On the other hand, the power model and the second-order quadratic interaction model had correlation coefficients of 0.4849 and 0.9707, respectively.

Enhancing thermostability of tryptophan hydroxylase via protein engineering and its application in 5-hydroxytryptophan production

5-Hydroxytryptophan (5-HTP), as the precursor of serotonin and melatonin in animals, can regulate mood, sleep, and behavior, which is widely used in pharmaceutical and health products industry. The enzymatic production of 5-hydroxytryptophan (5-HTP) from L-tryptophan (L-Trp) using tryptophan hydroxylase (TPH) show huge potential in application due to its advantages, such as mild reaction conditions, avoidance of protection/deprotection processes, excellent regioselectivity and considerable catalytic efficiency, compared with chemical synthesis and natural extraction. However, the low thermostability of TPH restricted its hydroxylation efficiency toward L-Trp. In this study, we aimed to improve the thermostability of TPH via semi-rational design guided by (folding free energy) ΔΔG fold calculation. After two rounds of evolution, two beneficial mutants M1 (S422V) and M30 (V275L/I412K) were obtained. Thermostability evaluation showed that M1 and M30 possessed 5.66-fold and 6.32-fold half-lives (t1/2) at 37 °C, and 4.2 °C and 6.0 °C higher melting temperature (Tm) than the WT, respectively. The mechanism behind thermostability improvement was elucidated with molecular dynamics simulation. Furthermore, biotransformation of 5-HTP from L-Trp was performed, M1 and M30 displayed 1.80-fold and 2.30-fold than that of WT, respectively. This work provides important insights into the thermostability enhancement of TPH and generate key mutants that could be robust candidates for practical production of 5-HTP.

The correlation study of several chemical extractants to assess plant copper uptake from tropical soils polluted with heavy metals

Glass-house experiments were conducted to study the correlation between Cu uptake by several plants and soil Cu extracted by several chemical extractants. Heavy-metal contaminated soils from Lampung, the southern part of Sumatra, Indonesia, with different levels of Cu were used. Eight different plants including amaranth (Amaranthus tricolor), caisim (Brassica chinensis var. Parachinensis), corn (Zea mays L.), land spinach (Ipomoea reptans Poir), lettuce (Lactuca sativa), napier grass (Penissetum purpureum), thorny amaranth (Amaranthus spinosus L.) and water spinach (Ipomoea aquatica) were employed. The uptake of Cu for amaranth is well predicted by N HCl, Buffered DTPA pH 7.30, N NH4OAc pH 7.00, and M CaCl2; for corn by N HCl and N NH4OAc pH 7.00; for land spinach by N HNO3, N HCl, Buffered DTPA, N NH4OAc pH 7.00, and M CaCl2; for napier grass by N HCl and Unbuffered DTPA. N HNO3 is good in predicting the uptake of Cu by land spinach, N HCl by amaranth, corn, land spinach, and napier grass; Buffered DTPA by amaranth and land spinach; Unbuffered DTPA by napier grass; N NH4OAc pH 7.00 by amaranth, corn and land spinach, and M CaCl2 by amaranth and land spinach. None of the tested methods is good in predicting the uptake Cu by caisim, lettuce, thorny amaranth, and water spinach

Numerical investigation of fluid flowing through rough fractures subject to shear

Comprehending fluid flow in rock masses is essential for modern underground engineering, including chemical energy extraction, nuclear pollutant remediation, and hydrocarbon utilization, complicated by shear-induced and surface roughness effects in fractures. This study employed numerical simulations to investigate the fluid flow behavior in fractures with different surface roughness under shear, where the shear direction is perpendicular to the flow direction. The nonlinear flow of the fluid is observed to have a strong correlation with the confining pressure (Pz), roughness (JRC), and shear displacement (u). The generation of eddy currents is frequently linked to the presence of flow channel intricacies and the velocity of flow at a microscopic scale. The Forchheimer equation could describe the process of nonlinear phenomena accentuation very well. The fracture under Pz caused a reduction in hydraulic transmissivity (T) due to compression. Furthermore, the T changes dramatically as the shear process progresses. Based on the analysis of the Forchheimer coefficient (β) and critical Reynolds number (Rec) parameters used to determine the response of nonlinear flow, it appears that an increase in Pz facilitates the transition of the fluid into a nonlinear flow state. Conversely, shearing has the opposite effect and reduces the tendency toward nonlinear flow.

Development of an Extraction Column using Machine Learning for the Prediction of Feed and Solvent to Obtain the Desired Extraction

The phrase "Industry 4.0" describes the fourth industrial revolution, which is characterized by the usage of digital technology to improve automation, connectivity, and efficiency across various industrial processes. The optimal column designs, suitable solvent selection, and extraction yield prediction are all aided by artificial intelligence (AI). Integration of AI technology with extraction columns is a significant step forward for industrial processes, bringing in a new era of intelligent systems that drive previously unheard-of increases in quality, efficiency, and environmental responsibility. The choice of acetone and water as the input solvents and Methyl Isobutyl Ketone (MIBK) as the extracting solvent results in an ideal configuration for the machine-learning design of an extraction column. With acetone's enhanced solubility and miscibility properties and water's versatility as a solvent, the two work well together. The extraction column was created using Python and concepts and methods based on chemical properties as well as machine learning. The intended extraction column may be implemented with the help of the Python code that is supplied. Based on parameters entered by the user, including extract temperature, raffinate temperature, extract mass flow rate, and extract temperature, it forecasts the temperatures and mass flow rates of acetone and water. By considering the properties of mass flow rates and temperatures, the projections ensure physical feasibility. Utilizing controlled data for training, the model applies a linear regression approach. The findings include the mass flow rate of acetone, the temperature of the water, and the water mass flow rate. The integration of state-of-the-art machine-learning techniques and Industry 4.0 is made possible in this work. This holds the promise of increased process optimization and efficiency in chemical extraction processes.

Optimizing Chitin Extraction and Chitosan Production from House Cricket Flour

Chitin and its derivative, chitosan, have diverse applications in fields such as agriculture, medicine, and biosensors, amongst others. Extraction is primarily conducted from marine sources, such as crustaceans, which have been the focus of process optimization studies. However, there are other sources that are more readily available, such as insects, where insufficient research has been conducted. The house cricket (Acheta domesticus) is a promising source for chitin extraction because of its high chitin content, availability, and short lifespan. Modern chemical chitin extraction methods have not been standardized due to the use of different reagents, molar concentrations, temperatures, and reaction times across publications. Therefore, in this study, the composition of Acheta domesticus cricket flour was determined: 2.62% humidity, 4.3% ash content, 56.29% protein, 13.35% fat, 23.44% carbohydrates, and 15.71% crude fiber content. After a drying, defatting, demineralization, deproteinization, and bleaching process, chitin extraction was performed, and chitosan was obtained via a deacetylation reaction. The demineralization process was standardized at 30 °C for 3 h using HCl 2 M, resulting in 95.85 ± 0.012%. The deproteinization process was optimized at 80 °C for 45 min using NaOH 2.56 M, yielding 43.23 ± 1.25%. Finally, the identity and physicochemical characteristics of the compounds were confirmed and determined through characterization with Fourier-Transform Infrared Spectroscopy, X-ray Diffraction, Scanning Electron Microscopy, and Differential Scanning Calorimetry.

Antimicrobial cellulose hydrogels against gram-positive and gram-negative bacteria

The development of hydrogels has a significant impact in fields such as tissue engineering and biomedical devices. The present study tests different cellulose particles extracted from plants to produce hydrogels and identify if these particles and hydrogels have antimicrobial properties. Nine cellulose particles from Ecuadoran biodiversity were obtained using an established chemical extraction protocol, characterized using known techniques, and evaluated for bacterial growth in-vitro. In addition, two particles of nine were selected to perform bacterial growth rates and bacterial adhesion assays. The bacterial growth rates with cellulose F1 and F53 were similar to the positive control (with antibiotic) with both Escherichia coli ATCC 25922 and TG1 strains. However, the results showed that the bacterial growth rate with seven of the nine cellulose particles was lower than the negative control (without antibiotics) suggesting antifouling properties. Based on the results using cellulose particles and hydrogels with antifouling properties, we prepared a plant extract to test the bactericide properties against Gram-positive (Staphylococcus aureus ATCC 25923) and Gram-negative (E. coli ATCC 25922) bacteria, evidencing the highest inhibitory effect at 40 and 60 mg/mL against S. aureus and E. coli, respectively. Lastly, we encapsulated the bactericide plant extract into the anti-fouling hydrogel. The results demonstrated that the combination of antifouling and bactericide properties could be an alternative approach for surface-modified cellulosic materials applications in the future.

Using Different Extraction Methods to Estimate Soil Salinity and Salt Type Changes and Their Effects on Soil Inorganic Carbon in Plowed Desert–Sierozem Soil

Understanding the actual soil salt ion content and salt type is one of the important prerequisites for determining the nature of saline soils and their development and utilization in drylands. Desert–sierozem soils are widely distributed in the plains between the northern piedmont of the Tianshan Mountains and the Gurbantunggut Desert in Xinjiang, Northwest China, which contain abundant calcium sulfate (gypsum) and calcium carbonate and are high-quality arable land resources for agriculture. These soils have been extensively reclaimed for farmland in recent decades. In this study, 10 plots of desert–sierozem soils with different tillage years in the Qitai Oasis plain area in the northern piedmont of Tianshan Mountain were studied. Three soil profiles were excavated in each sample plot with a depth of 1.2 m. A total of 30 farmland profiles were dug up and sampling was conducted according to the soil occurrence layers of the farmland. All the soil samples were predominantly sandy in texture, with more than 92% of the soil particles distributed between 0.05 and 0.25 mm, and less than 8% between 0.002 and 0.05 mm. Conventional water extraction (CWE) and intensified water extraction (IWE) were adopted to extract the water-soluble salt ions content of K+, Na+, Ca2+, Mg2+, Cl−, SO42−, HCO3− and CO32−, and this study compared the extraction results using two different methods with varying extraction strengths. Additionally, the calcium sulfate and calcium carbonate contents were obtained using the chemical extraction and mineral assay methods, respectively. Differences in the salts and salt types in desert–sierozem soils with different tillage years were analyzed, and the effect of calcium sulfate on the inorganic carbon sinks in plowed desert–sierozem soil was demonstrated. The findings indicated that the standard conventional extraction procedure involving a 5:1 water–soil ratio and 3 min of shaking was inadequate for accurately measuring the soil salt content in high calcium sulfate content desert–sierozem soils. With the IWE method, by increasing the water–soil ratio, prolonging the shaking time, and grinding the particles finer, the amount of water-soluble salts extracted using the IWE method was 5.83 times higher than that of the conventional method. Among them, the average content of IWE-Mg2+ increased by 123.41 mg kg−1 compared to CWE-Mg2+, the average content of IWE-Ca2+ was 3.82 times higher than CWE-Ca2+, with an average increase of 2931.59 mg kg−1, and the IWE-SO42− content was 5.96 times higher than CWE-SO42−, with an average increase of 8658.95 mg kg−1. A markedly negative correlation (p &lt; 0.01) was observed between calcium carbonate and calcium sulfate in desert–sierozem soils after tillage. The calcium sulfate content consistently decreased and calcium carbonate increased with an increasing number of tillage years. The pedogenic calcium carbonate content in desert–sierozem soils increased by an average of 10.86 g kg−1 after more than 40 years of cultivation, and the transfer of Ca2+ from calcium sulfate to calcium carbonate was identified as the cause of the increase in the soil inorganic carbon sink. Overall, a new method for extracting water-soluble salts was utilized for aridisols, which commonly consist of complex salt types. The results elucidate the changes in salinity within plowed desert–sierozem soils as well as the impact of soil salt on soil inorganic carbon sinks. Based on our research, desert–sierozem soils are well suited for agricultural farming because they contain high levels of sulfate, which is one of the important inorganic nutrients essential for crops, and it also provides a source of calcium for increasing inorganic carbon sinks. This study serves as a reference and scientific basis for soil carbon cycles in arid zones.

Methods for testing short- and long-term phosphorus fertilizing efficiency of products with varying solubility

Phosphorus (P) recovery from nutrient-rich side streams (NRSS) and derived products is crucial to ensure sustainable food production in the future and to enhance the circular economy, but the agronomic efficiency of these products needs to be validated to reach these targets. In this study, we used a Hedley fractionation scheme and the diffusive gradient in thin film (DGT) method to determine P availability in 83 NRSS and derived products originating from Finland, Sweden, and Germany. Furthermore, two independent short- and long-term growth experiments with barley (Hordeum vulgare L.) and ryegrass (Lolium perenne L.), respectively, were conducted to evaluate P availability in 15 selected NRSS. In addition to the DGT soil test, different fertilizer extractants, 2 % formic acid (FA), 2 % citric acid, and neutral ammonium citrate, were tested for predicting P availability in growth experiments. Livestock manures and slurries were found to contain a notable portion of labile P and were comparable to superphosphate (SP). Despite the low shares of labile P in struvite (7.2 %) and AshDec® (1.3 %), they exhibited P availability comparable to SP fertilizer, as indicated by DGT (99 % and 238 % of SP equivalence, respectively). This suggests that factors other than solubility influenced P availability in these side streams. The DGT method as a promising soil test predicted both short- and long-term P availability better than the selected conventional chemical extraction methods did. The 2 % FA extract exhibited the poorest performance, overestimating P availability in some nutrient sources while underestimating others in long-term. These findings enhance our understanding of P availability in potential raw materials for fertilizers, facilitating more effective P management strategies in the circular economy.

Novel positioning of Olea europaea L. (Olive) from farm to pharma indexed in pharmacopeia

The current study explores the transformative journey of L. (Olive) from its traditional role in random traditional medicine to its current positioning in pharmacopeia as a pharmaceutical preparation with defined dosage and indications. The comprehensive examination covers botanical and chemical profiles, traditional uses, pharmacological activities, extraction techniques, quality control, regulatory status, clinical studies, and future directions. Various analytical methods, including spectroscopic and chromatographic approaches, are discussed for quality evaluation and detection of adulteration. Additionally, the regulatory landscape, particularly within the European Union, is outlined, emphasizing the importance of accurate labeling and geographical origin disclosure. The regulatory status of L. varies across regions, ranging from restrictions due to safety concerns to inclusion in pharmacopeias as traditional herbal medicine or pharmaceutical preparations. A pivotal development highlighted is the incorporation of Olive into Egyptian pharmacopeia, signifying a significant shift towards evidence-based therapy and reinforcing its acceptance in mainstream healthcare.