Chemical Extraction Process Research Articles

Extraction of chitin and chitosan from Hermetia illucens breeding waste: A greener approach for industrial application

Sustainably exploiting the waste of the black soldier fly (BSF) to produce chitin and chitosan remains a challenge. This work valorizes the pupal cases of BSF for chitin and chitosan extraction. Four chemical extraction processes have been employed. Process 1, the standard method for this source, served as a control. Processes 2 and 3 were designed to assess and select the most effective delipidation method, while the optimized Process 4 involved autoclave conditions (121 °C-2.2 Bar). All chitin derivatives obtained were characterized by FTIR, SEM, XRD, 1H NMR, TGA, potentiometry, viscosimetry, and ICP-OES. Extraction using Process 4 (P4) proved to be the most efficient, demonstrating a deproteinization efficiency of 94.25 ± 0.6 % in a total reaction time of 1.15 ± 0.08 h and water consumption of 250 ± 26.86 L/kg, significantly lower than in other processes. In terms of yield, this process resulted in chitin and chitosan with respective yields of 34.74 ± 1.15 % and 83.33 ± 1.28 %, outperforming the other methods. Regarding physicochemical properties, P4 produced chitin and chitosan with improved thermal stability, with DTGmax values of 421 °C and 345 °C respectively. Additionally, the crystallinity of chitin was reduced by 25.68 %. For chitosan, the degree of acetylation (DA) was the lowest, while maintaining a high molecular weight of 220,378 g.mol−1. These results confirm that P4 is efficient and environmentally friendly, making it well-suited for industrial applications.

Development of Software for Hydrometallurgical Calculation of Metal Extraction

Hydrometallurgy plays a critical role in the metallurgical industry by providing an efficient method for extracting metals from ores and secondary materials using aqueous solutions. This approach is particularly advantageous for processing low-grade and complex ores, as well as secondary resources that cannot be effectively processed by traditional pyrometallurgical methods. The objective of this study is to develop specialized software to automate and optimize the calculations necessary for metal extraction in hydrometallurgical processes. The software integrates a complete computational framework for automating the various stages of the hydrometallurgical process, including ore composition initialization, total element mass calculations, and the determination of metal concentrations in both metal products (matte) and by-products (slag). The methodology involves the design and implementation of a web-based application using Django for the user interface and MySQL for robust data storage. The computational module, written in Python, automates the mathematical operations required to simulate complex chemical reactions and metal extraction processes. This module supports real-time processing and ensures accurate calculations for each stage of metal extraction, including leaching, extraction, re-extraction, sorption, and electrolysis. The software also features detailed tables and dynamic graphs that allow users to analyze the distribution of valuable metals and assess the influence of key operational parameters on extraction efficiency. The results show that the developed software successfully manages large datasets, enhances the precision of hydrometallurgical calculations, and minimizes the risk of human error. The implementation of the software leads to significant improvements in the economic efficiency of production by optimizing metal recovery rates and reducing operational costs. Additionally, it supports comprehensive process control by providing actionable insights into each stage of extraction, thus improving the quality of the final metal product. Overall, the software represents a significant technological advancement in the field, offering a scalable solution for industries aiming to streamline hydrometallurgical processes.

Advances in extraction, utilization, and development of chitin/chitosan and its derivatives from shrimp shell waste.

Shrimp consumption is in great demand among the seafood used globally. However, this expansion has resulted in the substantial generation and disposal of shrimp shell waste. Through literature search, it has been observed that since 2020, global scholars have shown unprecedented interest in shrimp shell waste and its chitin/chitosan. However, these new insights lack corresponding and comprehensive summarization and analysis. Therefore, this article provides a detailed review of the extraction methods, applications, and the latest research developments on chitin/chitosan from shrimp shells, including micro-nano derivatives, from 2020 to the present. The results indicate that chemical extraction remains the primary technique for the extraction and preparation of chitin/chitosan from shrimp shells. With further refinement and development, adjusting parameters in the chemical extraction process or employing auxiliary techniques such as microwave and radiation enable the customization of target products with different characteristics (e.g., deacetylation degree, molecular weight, and degree of acetylation) according to specific needs. Additionally, in pursuit of environmentally friendly, efficient, and gentle extraction processes, recent research has shifted toward microbial fermentation and green solvent methods for chitin/chitosan extraction. Beyond the traditional antibacterial, film-forming, and encapsulation functionalities, research into the applications of chitosan in biomedical, food processing, new materials, water treatment, and adsorption fields is gradually deepening. Chitin/chitosan derivatives and their modified products have also been a focal point of research in recent years. However, with the rapid expansion, the future development of chitin/chitosan and its derivatives still faces challenges related to the unclear mechanism of action and the complexities associated with industrial scale-up.

Research progress in chemical constituents and pigment extraction process of Carthami Flos

Carthami Flos(flowers of Carthamus tinctorius) with the effects of activating blood, dredging meridians, dissipating stasis, and relieving pain is one of the commonly used traditional Chinese medicines for promoting blood circulation and resolving stasis in clinical practice. So far, more than 210 compounds in Carthami Flos have been isolated and reported, including quinochalcones(safflower yellow pigments and red pigments), flavonoids, spermidines, alkaloids, polyacetylenes, and organic acids. Safflower yellow pigments, as the main water-soluble active components of Carthami Flos, is commonly obtained by the water extraction method, while red pigments are commonly obtained by the alkali extraction and acid precipitation method. In recent years, natural deep eutectic solvents as green solvents have demonstrated promising application prospects in the extraction and separation of pigments from Carthami Flos. This review systematically summarizes the chemical constituents of Carthami Flos and analyzes the extraction process of pigment components from Carthami Flos, aiming to provide a reference for further utilization of Carthami Flos resources.

Поэтапная комбинированная разработка золоторудных месторождений

Introduction. It is noted that in the Russian gold mining industry, the profitability of gold production is associated with the development of technologies for leaching it from substandard ores and enrichment waste. It is shown that the priority aspect of the development of substandard raw materials is to increase the yield of more gold with acceptable additional investments, predicted based on the analysis of the production function in the models of economic growth of enterprises. The purpose of the study is to substantiate the possibility of increasing the completeness of subsurface use during the phased development of reserves with a combination of chemical and mechanical extraction processes. Methods of achieving the goal include the use of literary and patent sources, production experience, laboratory and field experiments, modeling and technical and economic calculations. Results. The relevance of the development of a new raw material base is shown by the example of the reserves of the Sadonsky poly-metallic deposit. The information on the operations of hydrometallurgical and heap leaching of gold with bringing to the final product is detailed. An algorithm is proposed for the selection of options for the development of gold bulls. A model is presented to calculate the efficiency of phased field development over time. An optimized scheme of heap leaching of gold and indicators of gold extraction are presented. The scheme of electrochemical leaching of gold from sulfur-containing ores is recommended. The factors of the impact of mining on the environment at three stages of development are systematized. A model is presented for determining the efficiency of utilization of enrichment tailings and profits from the extraction of gold from enrichment tailings and metallurgy, taking into account environmental damage. The results of calculating the efficiency of gold extraction at the factory and leaching in blocks and cubes are presented. It is shown that the end-to-end metal recovery coefficient by leaching is comparable to the traditional technology of processing balance reserves. It is proved that when combining technologies at the stages of field development, a larger amount of metal is extracted from the subsurface. Conclusion: with a small increase in infrastructure modernization costs, combining traditional technology and leaching technologies, more metal is extracted from the subsurface.

Development of an Extraction Column using Machine Learning for the Prediction of Feed and Solvent to Obtain the Desired Extraction

The phrase "Industry 4.0" describes the fourth industrial revolution, which is characterized by the usage of digital technology to improve automation, connectivity, and efficiency across various industrial processes. The optimal column designs, suitable solvent selection, and extraction yield prediction are all aided by artificial intelligence (AI). Integration of AI technology with extraction columns is a significant step forward for industrial processes, bringing in a new era of intelligent systems that drive previously unheard-of increases in quality, efficiency, and environmental responsibility. The choice of acetone and water as the input solvents and Methyl Isobutyl Ketone (MIBK) as the extracting solvent results in an ideal configuration for the machine-learning design of an extraction column. With acetone's enhanced solubility and miscibility properties and water's versatility as a solvent, the two work well together. The extraction column was created using Python and concepts and methods based on chemical properties as well as machine learning. The intended extraction column may be implemented with the help of the Python code that is supplied. Based on parameters entered by the user, including extract temperature, raffinate temperature, extract mass flow rate, and extract temperature, it forecasts the temperatures and mass flow rates of acetone and water. By considering the properties of mass flow rates and temperatures, the projections ensure physical feasibility. Utilizing controlled data for training, the model applies a linear regression approach. The findings include the mass flow rate of acetone, the temperature of the water, and the water mass flow rate. The integration of state-of-the-art machine-learning techniques and Industry 4.0 is made possible in this work. This holds the promise of increased process optimization and efficiency in chemical extraction processes.

Large-scale atomistic model construction of subbituminous and bituminous coals for solvent extraction simulations with reactive molecular dynamics

Large-scale atomistic models for complex polycyclic aromatic hydrocarbon systems help understand the chemical properties and behaviors of complex feedstocks such as coal or petroleum. However, the development and utilization of large-scale models remain limited due to the difficulty in achieving the varied structural characteristics necessary to capture stochastic nature of these feedstocks. We demonstrate a systematic workflow to construct stochastic molecular systems from a broad analytical suite: high-resolution transmission electron microscopy (HRTEM), carbon-13 nuclear magnetic resonance spectroscopy (13C NMR), laser desorption ionization mass spectroscopy (LDI-MS), and elemental analysis. We present a model construction and analysis utility of a new Python-based module. We selected one subbituminous and three high-volatile bituminous coals to construct large-scale models (∼40,000 atoms). The constructed models were utilized to examine the affinity for solvent extraction (naphthalene or tetralin) and the effect of structural properties (e.g., aromatic cluster size, functional groups, and cross-linking) in reactive molecular dynamics simulations. Complex chemical reactions were monitored with bond order transitions, intermediates formation, and mass distributions. Reactive molecular dynamics simulations suggest a plausible chemical extraction process and products for the complex fossil feedstocks. The results indicated that radical formations with bond breaking of bridging oxygens and carbons were required at high temperatures to facilitate hydrogeneration and extraction of gas molecules from radical-free molecules. We observed that aliphatic chains of tetralin were easily decomposed and combined with radicals to form small size of molecules with aryl bonding, mainly increasing molecules in the 500–1000 Da, while naphthalene had little impact on chemical extraction process.

Phytochemistry, pharmacological effects and mechanism of action of volatile oil from Panax ginseng C.A.Mey: a review.

Panax ginseng (P. ginseng), a traditional and highly valued botanical drug, has been used for thousands of years and is known around the world for its uses in food, medicine, and healthcare. The comprehensive study of P. ginseng is crucial for the quality assurance of medicinal materials and optimal resource utilization. Despite being present in trace amounts, P. ginseng volatile oil has a wide range of chemical metabolites with important medicinal potential. The volatile oil has shown promise in defending the cardiovascular system, as well as in terms of its ability of antibacterial, anti-aging, anti-platelet coagulation, anti-inflammatory, support the nervous system nutritionally, and shield it from harm. Due to its low composition and lack of thorough investigation, P. ginseng volatile oil's therapeutic applicability is still restricted although it exhibited many benefits. This review aims to provide insights into the chemical composition, extraction processes, pharmacological effects, and mechanisms of action of P. ginseng volatile oil, and to provide theoretical support and guidelines for future research and clinical application.

Comparative Study of Starch-Based Bioplastic from Banana (Musa acuminata) peel (SBB) Incorporated with Commercialized and Extracted Chitin

Abstract: The study produced extracted Chitin from Asian Green Mussel (Perna viridis) and Golden Apple Snail (Pomacea canaliculate) shells using two-way chemical extraction process; deproteinization and demineralization. The extracted and commercialized Chitin were characterized through Fourier Transform Infrared (FTIR) Spectroscopy to determine and compare the functional groups present. Starch-based bioplastics were produced using banana peel starch (BPS) incorporated with commercialized chitin and chosen chitin extract which is from AGM. The bioplastics were tested for its morphology through Scanning Electron Microscopy (SEM), physical property through air test, chemical properties through biodegradability test, chemical reactivity test, water vapor transmission rate (WVTR) test, water absorption test and flammability test, and mechanical property through tensile strength test. Four treatments were carried out using 1%, 2%, 3%, and 4% by weight chitin addition. The control SBB does not contain any chitin. All treatments were prepared using constant amount of BPS, PVA solution and glycerol. Based from the results, chitin addition improves the strong acid resistance, water-barrier properties, and tensile strength of the SBB. It was found out that the best treatment was T3 of AGM which has a WVTR of 73.9868 g/m2 , dissolved in water in an average of 6.1783 days, and has a tensile strength of 64286.3777 Pa

Investigation on Akis granulifera (Coleoptera, Sahlberg, 1823) as a potential source of chitin and chitosan: Extraction, characterization and hydrogel formation

The majority of studies have focused on the industrial exploitation of marine fisheries waste through the production of natural bioactive bioploymeres such as chitin and chitosan. However, in recent years, beetles are increasingly attracting the interest of scientists as a source of chitin and chitosan for the preparation of hydrogels for sustainable engineering development. In the present work, we focus on the study for the first time a new Moroccan species of beetle (Akis granulifera Sahlberg, 1823), for the extraction of chitin and the elaboration of chitosan. A chemical extraction process was used. Then, physicochemical characterizations by FT-IR, SEM, XRD, 1H NMR, TGA/DSC, Potentiometry, Viscosimetry, and elemental analysis were performed. In addition, to evaluate its physicochemical quality, the elaborated chitosan is combined with alginate to form a hydrogel. This hydrogel was effectively characterized by SEM, DRX and FTIR to show the potential of chitosan from Akis granulifera in biomaterial applications.

Chemical extraction of Biodiesel and Parametric analysis of DI-Diesel Engine using Algae esterified Oil-Gasoline Blends

Of late, the automobiles of all categories are massively increasing which aggravates the dependency on fossil fuel thus, the accessibility of petro-fuel decreases relentlessly. Under these circumstances, the investigations are taking new dimensions to find alternative, renewable with cost-effective fuel exclusively biofuels. However, a newer approach has been attempted for the production of biodiesel from wild stuff of Spirulina algae which is explored directly in its aquatic systems of the natural environment. The chemical extraction process was attempted with wild biomass of algae via pre-treatment for obtaining promising lipid content then; the algal oil (lipid) was subjected to the production of biodiesel through sequential transesterification reactions. The obtained biodiesel was measured as 94.65% produced from 0.920kg of algal oil extracted out of 1.6kg of algal biomass in a stipulated duration of time (2.50hr) taken for attaining maximum temperature. Further, the produced biodiesel was evaluated for its distinctive properties and the outcomes were compared with ASTM standard specifications. All the results appeared to be within the standards limit thereby, the methodical analysis was done for the performance and emission parameters via interacting algal biodiesel in the experimental (DI) diesel engine test-rig configured with a variable injection pressure of 210, 220 & 230 respectively. The blending ratio of algal diesel and gasoline (AB10 & AB 20v/v) were prepared based on volume and the results were correlated with petro-diesel. The physico-chemical parameters like color, odour, viscosity, density, total acid number, initial boiling point, flash point, calorific value, cetane number etc. were found to be significant when compared with petro-fuel. Correspondingly, the interactive parameters like flash point, pour point, cloud point, carbon residue, sulfur, ash, and water contents were correlated with the standard specifications. The substantial approach was observed in emission characteristics such as hydrocarbon (HC), carbon monoxide (CO), oxides of nitrogen (NOX) respectively in engine-algal fuel interactions. Whereas, a slight increase than petro-diesel with respect to sulfur oxide (SOX) emission and smoke opacity were also recorded. However, the marginal increase in Brake specific fuel consumption (BSFC) and Brake thermal efficiency (BTE) was noticed and the brake power (BP) was found slightly lower than petro-diesel. In conclusion, the protocol of producing biodiesel from wild stuff biomass of Spirulina maxima was found to be techno-economically feasible and this potential algal biodiesel can be recommended for the current engine applications without any alterations.

Mixing Transport Mechanism of Three-Phase Particle Flow Based on CFD-DEM Coupling

The mixing transport courses of three-phase particle flows exist in some industrial applications, such as metallurgy material extraction, lithium electric slurry dispersion, and material mixing in the high-end chemical industry. Its mixing transport mechanism is a fluid–structure coupling dynamic issues with intensive shear and nonlinear characteristics, making the real-time prediction of the flow field face challenges. To address the above problem, a bidirectional fluid–structure coupling three-phase particle flow dynamic model is built based on the coupled computational fluid dynamics and discrete element model (CFD-DEM) to explore the mixing transport mechanism. An interphase coupling solution method is utilized to solve the interaction effects of the fluid and particle. Research results illustrate that the proposed method modeling can well reveal the mixing transport mechanism of the three-phase particle flows. Due to the additive effects of stirring speed, stirring blade size, and stirring blade structure, the flow field near the blade has a high-velocity gradient change, while the flow field away from the stirring blade has no significant change. When the particle material settles and accumulates to a certain extent, the particle movement is blocked, and the stirring speed of the particle material near the blade is reduced. The mixing effect of the particle material will be reduced near the wall. It can provide a valuable reference for particle flow transport and pattern identification and support technical support for lithium electric homogenate mixing, chemical extraction, and pharmacy process regulation.

Biopolymer manufacturing from pectin extraction of jackfruit waste to increase oil production in EOR

This research was conducted with the aim of making an alternative, nature-based biopolymer formed from the extraction of jackfruit peel pectin as a substitute for commercial polymers. The manufacture of this biopolymer is carried out by means of laboratory tests using conventional methods with a chemical extraction process on jackfruit skin. The raw material used is jackfruit skin (Artocarpus integer). Processing of jackfruit skin into pectin is carried out by several procedures such as extraction using citric acid, purification of pectin with 96% ethanol, FTIR characterization test, density test, viscosity test, shear rate test, and the effect of salinity. The FTIR test showed that pectin was formed after the extraction step, as evidenced by the structure of the pectin-forming groups which was similar to commercial pectin. The deposition of impurity particles contained in the pectin content is evidenced by the lack of optimal pectin to increase the viscosity of water. The increase in viscosity will occur as the concentration of pectin increases. Jackfruit pectin is able to survive in solutions that have high salinity, as evidenced by the higher the salinity of the solution, the thickening process continues.

Heterogeneous catalyst synthesis from Ribbon vittata shells and its characterization for biodiesel production from Blighia sapida seed oil

The global campaign to achieve net zero emissions have necessitated the switch from fossil fuels to biofuels. Owing to the limited resources and high overall production costs, the use of biobased materials has been considered as an economic approach to produce low carbon emitting biofuels. The utilization of heterogeneous catalyst synthesized from Ribbon vittata shells for biodiesel production from Blighia sapida seed oil is discussed in this paper. The crushed shells of Ribbon vittata was placed in a crucible and calcined in a furnace for 4 hr at a temperature of 900 °C to develop the heterogeneous catalyst. The calcined Ribbon vittata shells were characterized using scanning electron microscopy (SEM) and the energy-dispersive electroscope (EDS) to reveal the microstructure and elemental composition. Oil was extracted from Blighia sapida seeds using n-hexane solvent in a chemical extraction process. The biodiesel was produced from the extracted oil using the developed heterogeneous catalyst under selected optimal reaction conditions of 65 °C, 120 min, 2 wt.% catalyst content, and methanol to oil ratio of 9:1. Selected fuel properties of the produced biodiesel were determined using American Standards for Testing and Materials (ASTM) standards. The calcined Ribbon vittata shells showed regular rod-like porous particles, indicating its suitability as a catalyst material. The EDS analysis revealed Ca, O, and C to be the significant elements in the developed heterogeneous catalyst. An average biodiesel of 95.60% was obtained from the transesterification reaction. All the determined fuel properties were found to be within the ASTM D6751 limits for biodiesel. The utilization of calcined Ribbon vittata shells as a heterogeneous catalyst for transesterification of Blighia sapida oil is an economic approach to lower the cost of biodiesel and promote its adoption.

Research Progress on the Effect of Amomum Volatile Oil on Gastrointestinal Health

According to statistics, 10 million people worldwide die of gastrointestinal diseases every year, and the incidence of stomach diseases in the population is as high as 80%, among which 120 million people in China suffer from stomach diseases. Amomum villosum is a kind of traditional Chinese medicinal materials, is also a kind of medicine and food homologous raw materials, with moistening appetizer, warming spleen and diarrhea, Qi and pain relief effect. The volatile oil of Amomum villosum, the main active component of Amomum villosum has a variety of biological activities and pharmacological effects. Therefore, under the background of younger gastrointestinal health problems and more attention paid to gastrointestinal problems, it is of great significance to study the relevant effects of effective components of Amomum villosum, on gastrointestinal health. This paper focuses on the chemical composition, extraction process and specific mechanism of volatile oil of Amomum villosum, in gastrointestinal health, so as to provide certain reference and support for further research and development of volatile oil of Amomum villosum, in gastrointestinal health.

CURRENT STATE OF SOLAR PHOTOVOLTAIC PANELS WASTE MANAGEMENT OPERATIONS IN UKRAINE

The paper analyzes the steady trend of solar energy development, which is accompanied by the formation and accumulation of solar panel waste. This process requires the application of accomplished waste management and the development of waste disposal technologies. It was determined that the problem of waste solar photovoltaic panels becomes more urgent due to the military actions taking place on the territory of Ukraine. The general characteristics of waste for different types of panels are given. The heterogeneity of the materials of photovoltaic panels leads to an ambiguous classification and involves a combination of safe and dangerous components. It has been determined that photovoltaic panel waste can be divided into two hazard classes: hazardous waste and non-hazardous waste. The key points of solar panel waste management operations are characterized in accordance with the Law of Ukraine “On Waste Management” and EU Legislation. The main points of disposal of unusable photovoltaic panels with recovery of valuable raw materials are described. It is noted that there are significant differences in the material composition of crystalline silicon panels and thin film modules. It is noted that two processing types of PV modules are currently distinguished – coarse and fine, and the most effective steps in the preparatory stage of disposal are also determined. In general, PV panel waste disposal operations combine mechanical and chemical material extraction processes. The imperfection of the legislative and regulatory framework in the field of waste management, the increase in the ecological danger of the generation and accumulation of waste, the imperfection of the information base and waste monitoring, the insufficient development of the processes of organizing the processes of collection, accumulation and transfer of waste for disposal, the insufficiency of proposals for methods of processing and disposal of solar panels waste as a valuable resource of secondary raw materials are notable as the main problems in the field of waste management of photovoltaic panels in Ukraine. One of the main tasks for development is to increase the share of waste materials that are currently not disposed of, but can be used as secondary raw materials.

Research Progress on chemical constituents of traditional Tibetan herb Lamiophlomis rotata

Traditional Tibetan herb Lamiophlomis rotata is a traditional medicine with a long history of application. By consulting the relevant research literature of Lamiophlomis rotata, this paper summarizes the research progress of its chemical composition from the aspects of chemical composition, extraction process and quality control, so as to provide reference for subsequent activity research and product development.

Research Progress on chemical constituents of traditional Tibetan herb Lamiophlomis rotata

Traditional Tibetan herb Lamiophlomis rotata is a traditional medicine with a long history of application. By consulting the relevant research literature of Lamiophlomis rotata, this paper summarizes the research progress of its chemical composition from the aspects of chemical composition, extraction process and quality control, so as to provide reference for subsequent activity research and product development.

Validity of PubChem compounds supplied by Patentscope or SureChEMBL

Using fully automated chemical compound extraction processes, Patentscope and SureChEMBL identify chemical compounds in patents periodically delivered to PubChem. In this way, Patentscope and SureChEMBL became exclusive data sources for more than 14 million PubChem compounds. This study aimed to clarify whether the substance data supplied to PubChem by Patentscope or SureChEML could be partially incorrect. In PubChem, two random samples with each 50 PubChem compounds exclusively sourced either from Patentscope or SureChEMBL were generated. The validity check of these sample PubChem compounds led to the result that the extraction process resulted in erroneous compounds in 60 % of the cases. Although this result is based on a sample, it suggests that PubChem compounds that originate exclusively from Patentscope or SureChEMBL, to some extent, do not match the substances in the underlying patent specifications. Finally, the implications for PubChem users from the scientific and patent communities are discussed.

Aqueous and chemical extraction of saponin of Acacia concinna (Willd.) Dc.: An effective Bio-surfactant solution to extract silk fibroin from muga silk cocoons

• Extraction of silk fibroin by utilizing bio-surfactant saponin. • Aqueous and Chemical extraction process utilized to extract silk fibroin. • Acacia concinna (Willd.) Dc. is utilized for the extraction process. • Formation of micelles to eliminate the sericin from silk fibroin . • Analysis of the degummed fibers properties by SEM, XRD, TGA/DSC, tensile strength. Silk fibroin is generated from the silkworms and enclosed in a glue-like protein called sericin. In recent years, silk fibroin has been recognized for its significant biomedical significance, especially in tissue engineering, wound dressing, and drug and gene delivery. Numerous degumming methods have already been employed to extract the fibroin protein from the silk cocoons, which discharges large volumes of toxic admixture of sericin and chemicals through effluents. A sustainable, cleaner and environmentally promising silk fibroin extraction technique of muga silk have been investigated by utilizing the bio-surfactant saponin that has been isolated from the Acacia concinna (Willd.) Dc. in two different extraction processes aqueous extraction and chemical extraction. The degumming efficacy has been performed with respect to saponin concentration, degumming period, and temperature. After the completion of the degumming process, the silk samples were subjected to examination by SEM, XRD, TGA/DSC, and mechanical (tensile) capacity study. This novel and greener degumming treatment was potentially applied to extract the silk fibroin from the silk cocoons in a cleaned state. The toxicity level generated in the degumming process was observed to be very low owing to the adoption of non-toxic chemicals. Overall study ascertains about can be an effective usage of the extracted silk fibroin for medical applications because of its low toxicity.