Chemical Characterization Of Essential Oil Research Articles

Phytochemical Characterization, Fumigant and Contact Toxicity Activities of Four Essential Oils Against Eriophyid Gall Mite, Aceria pongamiae Keifer (Acarina: Eriophyidae).

Plant-derived essential oils (EO) offer a natural alternative to chemical pesticides for eco-friendly pest control approaches. Aceria pongamiae Keifer, a notorious pest that affects Pongamia pinnata (L.) Pierre has mainly been controlled using synthetic acaricides leading to resistance development and environmental issues. EOs provide a natural and biodegradable option for pest control, with a unique mode of action. This study evaluates the acaricidal efficacy of EOs-eucalyptus Eucalyptus maculata Hook (EEO), lavender Lavandula angustifola L. (LEO), peppermint Mentha piperita L. (PEO), and black pepper Piper nigrum L. (BPEO) against A. pongamiae for the first time. We investigated the biological activity of EOs using fumigation and contact toxicity assays at concentrations ranging from 0.1 to 1 % and 0.6 to 0.9 % respectively, overexposure periods of 24, 48 and 72 h. Chemical characterization of EOs was performed using GC-MS analysis. Eucalyptol (62.88 %), linalyl acetate (39.11 %), menthol (44.35 %), and caryophyllene (32.77 %) were the main components of EEO, LEO, PEO and BPEO respectively. After 24 h of observation, EEO (LC50=1.01 %) and after 48 and 72 h, PEO had the highest fumigant toxicity (LC50=0.71 and 0.29 % respectively). The BPEO showed the most contact toxicity after 24, 48 and 72 h (LC50=0.92, 0.68 and 0.46 % respectively). This work reinforces the selection of adequate essential oils for implementation in future pest control strategies.

Effects of Essential Oils on Biological Characteristics and Potential Molecular Targets in Spodoptera frugiperda.

Spodoptera frugiperda control methods have proved to be inefficient, which justifies the search for new control measures. In this search for botanical insecticides for controlling S. frugiperda, the following were evaluated: (i) the toxicity of essential oils (EOs) from Cinnamodendron dinisii, Eugenia uniflora, and Melaleuca armillaris; (ii) the effect of EOs on life table parameters against S. frugiperda; (iii) the chemical characterization of EOs; and (iv) the in silico interaction of the chemical constituents present in the three EOs with the molecular targets of S. frugiperda. The EO from E. uniflora had the lowest LD50 (1.19 µg of EO/caterpillar). The major compounds bicyclogermacrene (18.64%) in C. dinisii and terpinolene (57.75%) in M. armillaris are highly predicted to interact with the octopamine receptor (OctpR). The compound 1,8-cineole (21.81%) in M. armillaris interacts mainly with a tolerant methoprene receptor (MET) and curzerene (41.22%) in E. uniflora, which acts on the OctpR receptor. Minor compounds, such as nerolidol in C. dinisii and β-elemene in E. uniflora, are highly ranked for multiple targets: AChE, MET, OctpR, and 5-HT1. It was concluded that the EO from E. uniflora negatively affects several biological parameters of S. frugiperda development and is promising as an active ingredient in formulations for controlling this insect pest.

Bioactivity of essential oils in the control of Sitophilus ssp.

Brazil is the largest rice producer outside of Asia, and the large production of this grain in the Brazilian territory has aspects that affect its productivity. Among all the influencing aspects is the rice weevil, Sitophilus ssp. Considering the needs of the grain industry and the market that seeks products without toxic residues, the work evaluated the bioactivity of the essential oils of laurel (Laurus nobilis), eucalyptus (Eucalyptus citriodora), and mint (Mentha piperita) on the coleopteran Sitophilus ssp. The insects used were obtained from infested silos and kept in a breeding room at a controlled temperature. For testing the bioactivity of essential oils, essential oils were used at concentrations of 1%, 5%, and 10%. The adult insects used were placed on the plates and the count of live individuals (rice weevil) was performed at times of 15 min., 30 min., 60 min., 6h, 12h, and 24h. All tested oils showed efficiency in insect mortality. The chemical characterization of essential oils was performed by gas chromatography coupled with mass spectrometry, indicating the presence of oxygenated monoterpenes in the three essential oils analyzed.

Chemical Characterization and Chemotaxonomic Significance of Essential Oil Constituents of Matricaria aurea Grown in Two Different Agro-Climatic Conditions.

A comprehensive study on chemical characterization of essential oil (EO) constituents of a rarely explored plant species (Matricaria aurea) of the Asteraceae family grown in Saudi Arabia and Jordan was carried out. Analyses were conducted employing gas chromatographic approaches such as GC-MS, GC-FID, and Co-GC, as well as RT, LRI determination, and database and literature comparisons, on two diverse stationary phase columns, which led to the identification of a total of 135 constituents from both EOs. Oxygenated sesquiterpenes were found to be the most predominant chemical class of Saudi M. aurea EOs, in which α-bisabolol (27.8%), γ-gurjunenepoxide (21.7%), (E, E)-α-farnesene (16.3%), and cis-spiroether (7.5%) were present as major components. In contrast, the most dominant chemical class of Jordanian M. aurea oil was found to be sesquiterpene hydrocarbons, where (E, E)-α-farnesene (50.2%), γ-gurjunenepoxide (8.5%), (E)-β-farnesene (8.1%), and (Z, E)-α-farnesene (4.4%) were detected as chief constituents. It is interesting to mention here that Saudi and Jordanian M. aurea EOs showed quite interesting chemical compositions and were found to have different chemotypes when compared to previously reported M. aurea EO compositions.

Chemical characterization and antimicrobial activity of Baccharis vulneraria Baker essential oil against strains of microorganisms that cause cutaneous infections

Baccharis vulneraria Baker is used popularly for the treatment of skin infections. So, this study aimed investigate the antimicrobial activity and chemical characterization of the essential oil (EO) against microorganisms that cause cutaneous infections. The EO was analyzed by GC-MS. The antimicrobial test was performed using the serial microdilution method, and the antimicrobial activity was determined by the minimum inhibitory concentration against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Trichophyton interdigitale, Trichophyton rubrum, Fusarium solani and Fusarium oxysporum within the 32-0.0625 mg/mL concentration range. Were identified 31 EO compounds. Its major compounds are bicyclogermacrene, trans-cadin-1,4-diene, β-caryophyllene, and germacrene A. EO showed antifungal action against T. rubrum and T. interdigitale (2 and 4 mg/mL MIC, respectively). The growth of C. albicans, at 4 mg/mL, decreased by 50% compared to control. The oil had no significant potential for other microorganisms at the selected concentrations.

Chemical Characterization of Essential Oils With a Biocide Base for Conservation and Restoration

Essential oils (EOs) are acclaimed for their antimicrobial properties, leading to their multiple applications in various fields. In this work, four aromatic plants were used, namely thyme (Thymus mastichina L.), everlasting (Helychrysum stoechas Moench), European pennyroyal (Mentha pulegium L.) and fennel (Foeniculum vulgare Mill.). Hydrodistillation was the extraction method used, and the obtained extracts were composed of a variety of volatile molecules, mainly terpenoids and phenylpropenoids. The EO yields were determined, and the chemical composition of these natural products was obtained by gas chromatography-mass spectrometry (GC-MS) analysis. The yields varied greatly in the range of 0.99 to 4.27% (v/w). The two major chemical constituents of the EOs analysed by GC-MS were as follows: thyme – 1,8-cineole and champhor; everlasting – α-pinene and limonene; European pennyroyal – pulegone and isomenthone; fennel – trans-anethole and limonene. This preliminary study is an important contribution to the understanding of EO bioactive compounds that are under investigation to establish their ability to control the biocolonization of cultural heritage.
 Keywords: aromatic plants, chemical characterization, essential oils, green biocides

Larvicidal Activity and Chemical Characterization of Essential Oil Obtained from Callistemon citrinus

Larvicidal Activity and Chemical Characterization of Essential Oil Obtained from Callistemon citrinus

Chemical Characterization of Essential Oil of Local Endemic Lamium Bilgilii Celep (Lamiaceae)


 
 
 In this study, the chemical constitution of the essential oil of Lamium bilgilii Celep, a local endemic species of Turkey, was studied by GC-MS for the first time. The essential oil was obtained by hydrodistillation method and forty-three compounds were successfully identified representing 98.2% of the oil. Bicyclogermacrene (14.22%) and linalyl acetate (8.33%) and linalool (7.86%) were the most abundant components of the essential oil. L. bilgilii essential oil was rich in sesquiterpenes (35.8%) and oxygenated monoterpenes (26.6%). The results showed that bicyclogermacrene was found, for the first time, as a most abundant component in the Lamium oils.
 
 

Chemical composition of essential oils of different Siparuna guianensis chemotypes and their acaricidal activity against Rhipicephalus microplus (Acari: Ixodidae): influence of α-bisabolol

ABSTRACT In the present study, chemical characterization of essential oils (EOs) from different chemotypes of Siparuna guianensis was performed and their acaricidal activity against Rhipicephalus microplus evaluated. Leaves of S. guianensis were collected from three municipalities of the Zona da Mata region, Minas Gerais State, Brazil: São Miguel do Anta (SMA), Tocantins (TO), and Viçosa (VI). The EOs chemical compositions were analysed using gas chromatography-mass spectrometry. To evaluate acaricidal activity, larval packet tests (LPT), and adult immersion tests (AIT), were used. The major compound found in the SMA and TO samples was α-bisabolol (62.6 and 48.1%, respectively). In the VI sample, germacrene D was the predominant compound (25%). In the LPT, the highest concentration (20 mg/mL) of the VI sample caused a mortality rate of 81.1%, while for the TO and SMA samples, mortality was 100%. Pure α-bisabolol, acaused 100% mortality at a concentration of 10 mg/mL. The control percentage at the highest concentration (60 mg/mL) was 77.4, 87.8, and 98.6%, for the TO, SMA, and α-bisabolol samples, respectively. We demonstrate that there is variation in the chemical composition of the EOs of S. guianensis from different regions, and that the chemotypes rich in α-bisabolol presented greater activity against R. microplus.

Characterization of Wild Corsican Hops and Assessment of the Performances of German Hops in Corsican Environmental Conditions through a Multidisciplinary Approach

Hops (Humulus lupulus L.) is a species that grows spontaneously in Corsica, but the characterization of this species in this territory has not yet been investigated. The main objectives of this study are to explore the features of wild hops from Corsica and to determine the effect of the island terroir on some cultivars in the first year of growth. A multidisciplinary approach consisting of the genetic analysis, morphological comparison and chemical characterization of essential oils was carried out on four wild Corsican hops and three hop cultivars grown in Tettnang, Germany and Corsica, France. The morphological and GC-MS analysis of Corsican wild hops, set cluster coastal samples apart from the one far from the coast. This dissimilarity is supported by the SSR analysis by two of the three coastal accessions. The genetics demonstrate a proximity between the European noble cultivar Tettnanger and the mountain Corsican wild hop from Corte. The morphological comparison between German hops cultivated in Tettnang and in Corsican soil, and the GC-MS characterization of their essential oils’ chemical profiles, show different features between year 0 and year +1 for each sample. This multidisciplinary approach highlights an acclimatization of hop cultivars to the Corsican terroir one year after planting.

Extraction and chemical characterization of essential oil from Eucalyptus obtained by microwave and ultrasound

La extraccion por microondas libre de solventes y la asistencia con ultrasonido, como tecnologias emergentes ofrecen ventajas en la extraccion de aceites esenciales, siendo importante la investigacion del efecto sobre el rendimiento y la composicion del aceite esencial. Se evaluo la influencia de la humedad de las hojas y potencia de extraccion sobre el rendimiento del aceite esencial de eucalipto extraido por microondas libre de solventes, y una comparacion con la asistencia con ultrasonido. La extraccion se realizo por microondas a 300 y 600 watts, a 70, 80 y 90% de humedad en las hojas, seleccionando la potencia que evidencio mayor rendimiento del aceite esencial. Posteriormente, se evaluo el efecto del ultrasonido a 180 watts, 40kHz por 15 minutos sobre el rendimiento y la composicion quimica. Se identificaron los analitos obtenidos por ambos metodos de extraccion, mediante cromatografia de gases acoplado a un espectrometro de masas (CG-EM). El mayor rendimiento (0,55%) se obtuvo a 600 watts y 90% de humedad mediante microondas. La asistencia con ultrasonido incremento a 0,80% el rendimiento y 17 analitos adicionales en el aceite esencial. Se identifico como componente mayoritario al eucalipto l o 1,8-cineol, encontrado en 42,43% y 37,15% correspondiente a la extraccion con microondas libre de solventes y microondas con asistencia de ultrasonido. Palabras clave: aceite esencial; eucaliptol; 1,8 cineol; microondas libre de solventes; ultrasonido ABSTRACT The solvent-free microwave extraction and the ultrasound-assisted extraction, as emerging technologies, offer advantages in the essential oil extraction, being the research about the effect on the yield and chemical composition of the essential oil important. The influence of leaves moisture and extraction power on the yield of solvent-free microwave eucalyptus essential oil, and a comparison with the ultrasound-assisted extraction was evaluated. The extraction was done by microwaves at 300 and 600 watts, 70, 80 and 90% humidity in the leaves, selecting the power that shows the highest yield of the essential oil. Afterwards, the effect of ultrasound at 180 watts, 40 kHz per 15 minutes on the yield and chemical composition was evaluated. The analytes obtained by both extraction methods were identified, by gas chromatography coupled to a mass spectrometer (GC-MS). The highest yield (0,55%) was obtained at 600 watts and 90% humidity by microwaves. The ultrasound-assisted extraction increased to 0,80% the yield and 17 analytes more in the essential oil. Eucalyptol or 1,8 cineol was identified as a major component, found in 42,43% and 37,15% corresponding to solvent- free microwave extraction and ultrasound-assisted extraction. Key words: essential oil; eucalyptol; 1,8 cineol; solvent-free microwave; ultrasound.

Characterization of secondary metabolites of leaf and stem essential oils of Achillea fragrantissima from central region of Saudi Arabia

In this paper, a detailed study on chemical characterization of essential oils (EOs) constituents of leaves and stems of Achillea fragrantissima were carried out using GC-FID and GC–MS analysis employing two different stationary phase columns. In the studied plant which is collected from the central region of Saudi Arabia, trans-sabinyl acetate and trans-sabinol have been identified as the major components. To the best of our knowledge, these components are being reported for the first time as the major constituents in the EOs of A. fragrantissima. The results showed that chief chemical components of both (leaves and stems) oils were found to be almost same, however, their contents varied noticeably from each other. Among 108 identified components in the leaves oil, the major components were trans-sabinyl acetate (20.7 ± 0.00), trans-sabinol (14.9 ± 0.13), artemisia ketone (12.7 ± 0.46), santolina alcohol (10.1 ± 1.30), β-sesquiphellandrene (5.5 ± 0.01), β-thujone (5.1 ± 0.11). Whereas, in the stem oil 85 components were identified and trans-sabinyl acetate (24.0 ± 0.19), trans-sabinol (19.2 ± 0.01), artemisia ketone (16.3 ± 0.74), santolina alcohol (10.4 ± 1.50), and β-sesquiphellandrene (4.8 ± 0.01) were found to be the major components. Among the identified components form both oils, 23 components were specific to only leaves oil, whereas 85 components were found to be common in both oils.

Neuroprotective effect of the essential oil of Lavandula officinalis against hydrogen peroxide-induced toxicity in mice

Background: Brain is the main organ that manages all other organs and has significant oxygen requirements, which makes it vulnerable to reactive oxygen species, thus causing different neurological disorders. The aim of this work is the evaluation of Lavandula officinalis essential oil neuroprotective effect against hydrogen peroxide (HP)-induced toxicity in mice. Materials and Methods: Essential oil was extracted by hydrodistillation using a Clevenger-type apparatus. Essential oil analysis is performed using gas chromatography-mass spectrometry (GC-MS). Biological activity evaluation carried out the ferric reducing antioxidant power test, deoxyribonucleic acid (DNA) fragmentation assay, the histopathological study of the brain, and determination of antioxidant enzyme activities. Results: Chemical characterization of essential oil using GC-MS identified 47 compounds, accounting for almost 80% of the total oil and indicates the occurrence of monoterpenes and sesquiterpenes. The identified major compounds are pentanone (16.55%), propanal (15.89%), methyl ethyl ketone (13.51%), naphthalene (10.81%), terpinen-4-ol (6.55%), cyclopentanecarboxylic acid (4.77%), and isoborneol (2.27%). This study allowed us to investigate the effects of HP on brain function in Mus musculus adult mice by assessing DNA degradation, cell morphology, oxidative balance and brain weight variation. Furthermore, we have highlighted the beneficial effects of L. officinalis essential oil, which could significantly counteract all these alterations by its active compounds, which are endowed with potent biological activities. Conclusion: We can conclude that HP-induced damage in histomorphological changes in mice brain, significant atrophy, as well as an important alteration of the genetic expression.

Chemical characterisation of essential oils of leaves of two Solanaceae: Solanum rugosum and Solanum erianthum from Côte d’Ivoire

This study made it possible to characterise by GC (in combination with retention indices), GC-MS and 13C NMR, the chemical composition of the essential oils from Solanum rugosum and Solanum erianthum, two Solanaceae of the Ivorian flora. The two essential oils were characterised by a very high proportion of sesquiterpenes. Specifically, the essential oil of S. rugosum was dominated by (E)-β-caryophyllene (33.7%), β-elemol (19.8%) and germacrene D (14.4%), while that of S. erianthum was mainly composed of α-humulene (38.6%), β-elemol (17.8%) and (E)-β-caryophyllene (16.7%). The chemical composition of Solanum rugosum is described here for the first time.

Essential Oil and Hydrosol Extract Chemical Profile, Antioxidant and Antimicrobial Potential of Daphne gnidium L. from Algeria

Essential oils (EOs) and hydrosol extract from plants always show a wide range of interesting biological activities. The present study was performed to assess for the first time, the chemical composition and some bioactivities of EO and hydrosol extract from Daphne gnidium L. growing in Algeria. The chemical characterization of EO and hydrosol extract was carried out by GC/FID and GC/MS analysis. The antioxidant activities were evaluated by DPPH radical scavenging and by FRAP tests. Also, antimicrobial screening of these extracts were performed using the agar disc diffusion test. Ninety-nine components in EO and thirty-two components in hydrosol extract accounting for 95.5 % and 81.4 % of the total extract composition were identified. The main constituents were carvone (12.7 %) followed by spathulenol (11.2 %) in EO, while the major compounds present in the hydrosol extract were carvone (10.9 %) and carvacrol (10.9 %). The results of antioxidant activity of hydrosol extract could be considered more interesting than EO. The data of the antimicrobial assays showed that all strains were sensitive to these extracts where EO displayed a very strong antibacterial activity mainly for E. coli, P. aeruginosa and S. aureus bacteria. Up to date, the current study is the first scientific report of antioxidant and antimicrobial activities as well as the chemical composition of the EO and hydrosol extract of Algerian D. gnidium. The obtained results could promote the potential of D. gnidium as a promising source of bioactive compounds with a possible application as new natural antioxidant and antimicrobial agents.

Caracterización química y actividad antibacteriana del aceite esencial de Mangifera indica L. de tres regiones de Venezuela

Los aceites esenciales (AE) de las hojas de Mangifera indica L. recolectadas en los estados Mérida (M), Barinas (B) y Portuguesa (P), Venezuela, fueron obtenidos por el método de hidrodestilación, utilizando la trampa de Clevenger, obteniéndose 0,1 mL (0,0025%), 1,4 mL (0,035%) y 1,0 mL (0,025%), respectivamente. Los AE se caracterizaron por el método de cromatografía de gases acoplado a espectrometría de masas (CG/EM), se identificaron 30 compuestos en la muestra recolectada en M, 24 en B y 14 en P, siendo los mayoritarios en M: β-selineno (22,56%), α-gurjuneno (14,66%), β-cariofileno (10,40%), en B: β-cariofileno (36,32%), α-humuleno (22,71%), α-gurjuneno (21,43%) y en P: β-cariofileno (36,07%), α-gurjuneno (22,55%) y α-humuleno (21,24%). Debido al rendimiento, solo se determinó la actividad antibacteriana en los AE de B y P, por el método de difusión en agar con discos, frente a bacterias de referencia internacional (Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 23357 y Pseudomonas aureginosa ATCC 27853). Ambos AE inhibieron el desarrollo de S. aureus y E. faecalis con una concentración inhibitoria mínima de 200 µL/mL y 300 µL/mL, respectivamente. Este es el primer estudio comparativo y actividad antibacteriana del aceite esencial obtenido de las hojas de M. indica L. de tres regiones de Venezuela.

Antimicrobial Activity and Chemical Composition of Essential Oil Extracted from Solidago canadensis L. Growing Wild in Slovakia.

Plant essential oils (EOs) are one of the most relevant natural products due to their biological, medicinal, and nutritional properties. The promising biological effects of many plants EOs encourage researchers to study their biochemical properties to be used as possible natural alternatives for commercial pesticides and not only as herbal medicines. The current research has been conducted to study the microbicide effect of Solidago canadensis L. EO to control some common plant diseases caused by several postharvest phytopathogenic fungi (Monilinia fructicola, Botrytis cinerea, Aspergillus niger, and Penicillium expansum) in comparison with Azoxystrobin as a large spectrum fungicide. The antibacterial activity has been carried out against some phytopathogenic bacteria (Bacillus megaterium and Clavibacter michiganensis (G+ve) and Xanthomonas campestris, Pseudomonas fluorescens, and Pseudomonas syringae pv. phaseolicola (G−ve)) compared to the synthetic antibiotic Tetracycline. Minimum inhibitory concentration was carried out to determine the lowest effective EO dose using a 96-well microplate. The cell membrane permeability was also evaluated by measuring the electric conductivity (EC) to examine the possible mechanisms of action of S. canadensis EO. Chemical characterization of EO has been carried out using gas chromatography and mass spectrometry (GC-MS). Thirty-two identified components in S. canadensis EO presented 97.7% of total compounds in EO. The principal compounds were identified as germacrene D (34.9%), limonene (12.5%), α-pinene (11.6%), β-elemene (7.1%), and bornyl acetate (6.3%). In addition, S. canadensis EO demonstrated promising in vitro antimicrobial activities against the majority of tested phytopathogens at all tested concentrations.

Chemical characterization of essential oil and extracts of Daucus carota seeds and their antifungal activity against wheat fungi

Chemical characterization of essential oil and extracts of Daucus carota seeds and their antifungal activity against wheat fungi

Actividad antifúngica de Cymbopogon citratus contra Colletotrichum gloeosporioides

The objective of this research was to evaluate in vitro the inhibitory activity of essential oils from fresh leaves of Cymbopogon citratus (lemongrass) against Colletotrichum gloeosporioides in yam. The research was conducted in the department of Sucre, Colombia. The essential oils of C. citratus were collected in the municipalities of Sincelejo, La Union and Sampues, in September and October 2015. The essential oil was extract from fresh leaves using the microwave-assisted hydrodistillation. Concentrations of 5000, 8000 and 10 000 ppm of each essential oil were prepared. An absolute control, a positive control (benomyl 1 g/l) and a negative control was used. An inhibitory activity was obtained by using the direct seeding on surface of the potato-dextrose-agar method and it was expressed as percentage of inhibition rate. The chemical characterization of essential oils was performed by gas chromatography coupled to mass spectrometry. The highest percentages of antifungal index were observed in the lemongrass from Sincelejo at concentrations of 5000, 8000 and 10 000 ppm, after in La Union at 8000 and 10 000 ppm, and finally at Sampues at 10 000 ppm, with a value of 97.77%; the effect was similar to the positive control with benomyl 1 g/l. Citral was the main constituent of the essential oils extracted. The essential oils obtained from the three municipalities showed antifungal activity against C. gloeosporioides.

Chemical characterization of essential oil from the leaves of Callistemon viminalis (D.R.) and Melaleuca leucadendron (Linn.)

ObjectiveTo isolate and identify the compounds in the essential oils from the leaves of Callistemon viminalis (D.R.) and Melaleuca leucadendron (Linn.) collected in Dakar, Senegal. MethodsThe essential oils from the leaves of these two myrtaceaes were extracted by steam distillation and analyzed by gas chromatograph and gas chromatography–mass spectrometer. ResultsA total of 34 constituents were identified in the oil of Callistemon viminalis and the major compounds were 1.8-cineole (58.12%), limonene (9.72%), α-terpineol (9.56%), geranial (6.02%), δ-elemene (3.53%), myrcene (2.96%) and α-pinene (2.49%). For the essential oil of Melaleuca leucadendron, 43 constituents were identified, and 1.8-cineole (28.87%), epiglobulol (23.06%), α-pinene (12.22%), limonene (11.65%) and α-terpineol (7.06%) were major compounds. ConclusionsConsidering properties of the identified major compounds, essential oils of both studied myrtaceae could be used in the medicine field including the food, pharmaceutical and cosmetic industry.