Characterization Procedure Research Articles

A consensus platform for antibody characterization.

Antibody-based research applications are critical for biological discovery. Yet there are no industry standards for comparing the performance of antibodies in various applications. We describe a knockout cell line-based antibody characterization platform, developed and approved jointly by industry and academic researchers, that enables the systematic comparison of antibody performance in western blot, immunoprecipitation and immunofluorescence. The scalable protocols, which require minimal technological resources, consist of (1) the identification of appropriate cell lines for antibody characterization studies, (2) development/contribution of isogenic knockout controls, and (3) a series of antibody characterization procedures focused on the most common applications of antibodies in research. We provide examples of expected outcomes to guide antibody users in evaluating antibody performance. Central to our approach is advocating for transparent and open data sharing, enabling a community effort to identify specific antibodies for all human proteins. Mid-level graduate students with training in biochemistry and prior experience in cell culture and microscopy can complete the protocols for a specific protein within 1 month while working part-time on this effort. Antibody characterization is needed to meet standards for resource validation and data reproducibility, which are increasingly required by journals and funding agencies.

Characterisation of near pure mode II quasi-static and fatigue delamination behaviour of a carbon-glass interface using a pure-moment-loaded DCB test rig

This work covers the characterisation of delamination growth in a carbon-glass interface, representative of the one in a wind turbine blade. A pure-moment-loaded Double Cantilever Beam (DCB) test rig is used under quasi-static and fatigue loading. A new fatigue characterisation procedure is adopted by real-time controlling the prescribed energy release rate G. This procedure enables sweeping any desired G range at a user-defined rate independently of the compliance of the specimen. A finite element simulation using a cohesive zone model confirms the testing was performed at near pure mode II conditions. From the quasi-static tests, the onset and steady-state fracture toughnesses are characterised. Fibre bridging is observed, which is believed to explain the characterised R-curve effect. From the fatigue tests, Paris’ law is characterised in a wide Gmax range in each test. The characterisation of the quasi-static and fatigue fracture behaviour of the carbon-glass interface is thus successfully achieved.

Minimizing the carbon footprint of 3D printing concrete: Leveraging parametric LCA and neural networks through multiobjective optimization

Concrete 3D printing proposes an off-site industrial process allowing to deposit material only where required. However, most mixture design methods struggle to perform, which is why a majority of 3D printing materials display high clinker contents. This study proposes a reproducible methodology for tailor-made 3D printing materials. Applied to a low-clinker quaternary blend, an iterative optimization process leads to a significant reduction of labor in material tuning. It involves life cycle assessment and artificial neural networks as objective functions in the Pareto selection of best-performing solutions. Following the constitution of an 18-mixture database with 6 independent variables and 5 objective functions, printable mortars of different strength classes are designed within 2 to 4 active learning runs. Consequently, this optimum-driven technique allows to rapidly converge toward low-carbon solutions for 3D printing, using local materials and custom characterization procedures.

Improving the stability of high-speed trains using an innovative passive yaw damper

Ensuring lateral stability is essential for achieving excellent operation safety and ride comfort of high-speed trains. However, complicated operational environments, particularly various wheel-rail contact geometries, would result in the occurrence of hunting instability. This study presents an innovative passive yaw damper to improve the stability of a high-speed train undergoing extreme wheel-rail contact conicities. A sensitivity analysis of the vehicle stability is first performed to demonstrate the importance of the dynamic stiffness of yaw dampers, promoting the application of the innovative damper. Experimental characterisation procedures enable the detailed investigation into the static and dynamic characteristics of the innovative damper, which are compared with those of conventional passive dampers to highlight the difference. To simulate the dynamic behaviour of the yaw damper, a numerical modelling method based on the Maxwell model is proposed. Using this method, the effectiveness of the innovative damper is assessed in terms of improving the vehicle stability at various wheel-rail conicities. The results show that the innovative yaw damper can ensure carbody stability at low conicity and bogie stability at high conicity, superior to conventional dampers, which fail to balance the vehicle stability at distinct conicities and usually result in hunting instability in specific wheel-rail contact states.

Bacteriology and Physicochemical Analysis of Borehole Water in Some Hostels of a Tertiary Institution in Port Harcourt, Nigeria

Several factors influence the quality of water for human consumption. Most of these factors are more of anthropogenic origin than nature and thus exercises an untold health effect on the end-users of such water body. Bacteriology and Physicochemical Analysis of Borehole Water in Some Hostels of a Tertiary Institution in Port Harcourt, Nigeria. Water samples were collected from six (6) hostels for each study period, and subjected to standard laboratory procedures to analyze for parameters such as pH, nitrite, nitrate, dissolved oxygen (DO), electrical conductivity (EC), Phosphate, as well as standard procedures for antibiotics susceptibility and molecular characterization. Result of the physicochemical parameters showed that the parameters ranged from 5.26±3.53 mg/L to 7.61±0.01 mg/L forpH; 0.61±0.01 to 2.56±0.01 mg/L for Dissolved Oxygen (DO); 96±1.41 μS/cm to 503.5±0.71μS/cm for electrical conductivity; 4.56±0.03 mg/L to 20.3±0.01 mg/L for nitrate; 0.003±0mg/L to 0.03±0mg/L for nitrite; 0.12±0mg/L to 0.17±0mg/L for phosphate. Statistical evaluation showed there was a significant difference (p < 0.05) between the mean values of physicochemical parameters studied, except for pH that recorded no statistical difference (p > 0.05). From the study, 5.6% of Staphylococcus species were resistant to Pefloxacin and Rocephin, while 5.9% of Bacillus species were resistant to Ampiclox. Similarly, 3.33% of Salmonella species were resistant to Augmentin, while the rest of the gram negative isolates were either susceptible or within an intermediate range of susceptibility to the antibiotics tested. Molecular characterization of some bacterial species isolated had pairwise identity with Klebsiella pneumonia strain CP27 and Escherichia coli strain RIZHAO 498.1, Staphylococcus haemolyticus strain SH1. The study has shown that the physicochemical water quality variables were all within the WHO limits, with bacterial species having high percentage susceptibility to the antibiotics tested. Regular water quality monitoring is however recommended to ensure continuous access to water of good quality in hostels within the university community.

Outdoor Performance Monitoring Method for Degradation Studies of Perovskite Modules

ABSTRACTThis paper presents an outdoor performance monitoring method for degradation studies of perovskite modules, focusing on a large‐area perovskite module (81.9 cm2) over a long‐term monitoring campaign. The module underwent an industrial lamination process to prevent long‐term degradation from environmental factors. The characterization procedure involved degradation correction and determining the temperature coefficients and electrical parameters of the module using initial days of measurements. The results demonstrated temperature coefficients for Isc, Voc, and Pm (α′, β′, and γ) of −0.071%·K−1, −0.119%·K−1, and −0.113%·K−1, respectively, indicating a minimal temperature influence on this technology compared with conventional ones. Using this coefficient, the STC electrical parameters were retrieved from 1‐min power output data, resolving the uncertainty of the indoor/outdoor IV curve measurements caused by the curve scan direction (JV hysteresis effect). We also highlight the initial remarkable capacity recovery effect of almost 16% during the first 2 days of operation. Additionally, a procedure that includes the IV curves analysis taken every 10 min and their translation to standard conditions has been implemented to evaluate the degradation of the module over the long‐term outdoor campaign. The results show three different trends over the period.

Nano-priming of Vigna radiata seeds with opuntia stricta-derived γFe2O3 nanoparticles

The experimental investigation aimed to enhance sustainable and biocompatible nano-agriculture by examining the possible effects of biosynthesized nanoparticles (γFe2O3 NPs) on seed growth and growth parameters of Vigna radiata. The outcomes of various characterization procedures such as FTIR, XRD, UV, and Fe-SEM were used to determine the successful generation of γFe2O3 NPs (nanoparticles), which were capped with multiple phytoconstituents contained in the Opuntia stricta extract. γFe2O3 NPs were synthesised, as evidenced by the peak's presence, having a mean crystallite size of 13.9 nm, and were successfully detected using X-ray diffraction (XRD). Crucial Energy Dispersive X-ray Spectroscopy (EDS) testing also yielded unambiguous proof of the element iron's presence. Raman spectroscopy was used to confirm the synthesis of pure maghemite nanoparticles. Wide areas around 350, 500, 670, and 1330 cm-1 values verify the creation of pure maghemite. TGA analysis was performed to check the stability of nanoparticles in which the residual weight was found to be 87.62%. TEM images confirm the spherical shape, and the mean size of the nanoparticles was found to be 12.28 nm. Through a number of tests, the analysis showed that the nanoparticles have a strong antioxidant capacity. This experiment aimed to determine how biogenic γFe2O3 Nanoparticles affected Vigna radiata seed germination. Measurements of shoot and root lengths, seed vigour index, germination rates, mean daily germination, and peak value (PV) were all included of the assessment. There was a total of four distinct γFe2O3 NPs concentrations examined (25, 50, 75, and 100 μg/mL). The penetration of the γFe2O3 NPs affects the germination and growth of the Vigna radiata seeds. When compared to untreated seeds, the germination index values for Vigna radiata seeds that underwent effective treatment with γFe2O3 NPs significantly improved.

Inverse parameter estimation for the white–metzner equation using extrudate diameter measurements

Abstract This study presents a method to determine the elastic modulus of the White–Metzner (WM) viscoelastic model, which is used to describe polymer melt flow. In processes involving polymeric liquids, elastic effects are essential for accurate simulations. In particular, phenomena such as melt swelling after extrusion and upon gate passage in injection molding are critical to process simulation. However, systematic procedures for material characterization remain underdeveloped since implementation of an appropriate material model is challenging due difficulties inherent in parameter determination. In this work, to account for the shear thinning behavior, which is essential when handling polymeric liquids in current processes, while also incorporating elastic properties, the WM equation was employed. The method employs the post-extrusion swelling phenomenon where the diameter of the extruded material expands after exiting the capillary die. It integrates melt swell measurements with numerical simulations based on the WM equation and highlights the importance of considering gravity in the simulation. A laser scanning microscope is used to measure the extruded diameter while adaptive multi-objective optimization identifies unknown parameters in the model to align the simulation results with the measured diameter. This approach successfully determined the elastic modulus of the PET-PEN copolymer, establishing a system for determining the viscoelastic properties of polymer melts.

Procedure for Fabrication and Characterization of Van-der-Waals Heterostructures

In this work we provide a step-by-step description of the technique for manufacturing various van der Waals heterostructures. First, we discuss the procedure to obtain monolayer and few-layer flakes from layered materials, in particular from graphite and hexagonal boron nitride. Next, we consider different approaches to the assembly depending on the required final device. Further, we describe in detail the procedure for making ohmic contacts and give the parameters for plasma chemistry and metal deposition. We observe the field effect in transport measurements but a number of features – a strong shift of the charge neutral point from the zero-gate voltage, a large resistance away from the charge neutral point, and low mobility – indicate a problem with the quality of the resulting devices. Nevertheless, one of the fabricated devices demonstrates reasonable quality – the maximum mobility is estimated at 15000 cm2V–1s–1, the magnetic field dependences demonstrate the quantum Hall effect, which is standard for high-quality graphene. Unexpectedly, scanning electron microscope images of the resulting devices reveal a large amount of contamination on the surface of the flakes, which may explain the corresponding quality of our devices. Preliminary results of flakes cleaning with chemical compounds and thermal treatment are given.

The measurement of tire-road noise: how does standardization contribute to the development of low noise pavements in Europe

Low-noise road pavements are among the most effective solutions for reducing road traffic noise emission at the source. There was a significant international effort over the past years for developing and standardizing methods for measuring tire-road noise emission, essentially Statistical Pass-By (SPB) and Close ProXimity (CPX) methods. The dedicated Working Group of the European Committee for Standardization (CEN/TC227/WG5) recently investigated how these standards are used in practice and how they contribute to the implementation of low-noise pavements. For this, a survey was launched and 14 European countries responded on their practices. The survey showed a large diversity of use, although all refer to the measurement methods SPB, CPX. Based on these methods, some countries have developed national regulations or reference guidelines to specify and assess the acoustic performance of road surfaces. These procedures are, however, significantly different and lead to varying definitions of low-noise pavements, which prevents sharing knowledge, experience and data. This context justifies the ongoing development within CEN/TC227/WG5 of a standard procedure for the acoustic characterization of road pavements based on existing measurement methods. The paper will present and analyze the results of the survey, then will present the ongoing work in CEN.

A high-resolution acoustic goniometer for characterizing sound diffuser reflections

Noise control engineering and architectural acoustics practice have widely applied diffusing elements and devices to create surface scattering and diffuse reflections. An acoustical goniometer in the form of a circular microphone array has been used to characterize scattered responses for various types of diffusing elements/devices. This research is also intended for experimental validation of diffraction simulations of diffusing devices based on the physical theory of diffraction (PTD). To cope with experimental challenges, particularly for the research in the physical theory of diffraction, an acoustic goniometer must achieve a high enough angular resolution in addition to fulfilling far-field requirements. This paper discusses practical implementations and experimental results of portable goniometers with a radius of up to five meters and an angular resolution of 1.25 degrees. The practical implementation is intended to be easily deployable in empty, indoor spaces of sufficiently large dimensions for scattered reflection characterization. This paper discusses an effort of increasing experimental efficiency of characterization procedures for oblique incidences.

Defining pediatric neurosurgery in low-income countries: a cross-sectional study in Ethiopia.

Pediatric neurosurgical practice is prevalent in most low- and lower-middle-income countries but lacks comprehensive documentation of practice patterns, demographics, and case variety. This study aimed to present the current state of pediatric neurosurgery in Ethiopia, including workforce characterization, case variety, and relevant procedures. A survey was developed and distributed to all Ethiopian fully trained neurosurgeons (n = 50). Survey questions assessed sociodemographic variables, level of training, case variety, and neurosurgical practice. Statistical analysis was conducted to describe the current practice of pediatric neurosurgery. A total of 45 neurosurgeons responded (90%). Three respondents (7%) were women. There was only 1 fellowship-trained pediatric neurosurgeon, while most neurosurgeons were general neurosurgeons who served a pediatric patient population. Most neurosurgeons (56%) worked in the capital city, Addis Ababa, while another 13% worked in other urban settings. The top three indications for a pediatric neurosurgical procedure were neural tube defects (NTDs) (96%), hydrocephalus (93%), and trauma (60%). NTD-associated hydrocephalus was the most common hydrocephalus type seen (71%). The most common procedure for hydrocephalus was shunt insertion (96%). A prenatal diagnosis of NTD was made in < 10% of cases, as reported by 84% of respondents. The study highlights Ethiopia's need for more pediatric neurosurgeons. Suggested strategies to facilitate subspecialty training include the establishment of a fellowship program facilitated by the implementation of a nationwide pediatric neurosurgery registry. Promoting efforts for early diagnosis and treatment of pediatric conditions coupled with NTD prevention initiatives could improve pediatric neurosurgical care in Ethiopia.

Graphene-Based Nano Drug Delivery of Curcumin for the Treatment of Dengue Fever.

Graphene, which is defined as a single-layered sp2 hybridized carbon atoms placed in a honeycomb-like crystal lattice when converted to graphene oxide, has proven its worth by showing its tremendous capacity in controlling the release rate of drugs by entrapping in the numerous pockets present inside it through π-π stacking interaction when used as a nanocarrier. In this experiment graphene oxide was synthesized through traditional Hummer’s method and was used as a nanocarrier to target nanosized drugs, which was prepared by loading curcumin drug into the PVP functionalized graphene oxide by using necessary procedures. After the successful confirmation of the stability, functionalization, and compatibility through various characterization procedures and effective drug loading into the systems, the release behavior of the formulations has been checked through dialysis bag diffusion technique using potassium phosphate buffer saline (pH 7.2, 4.0 and 9.2) for selection of the best medium for determination of the successful sustained-release behavior of the drugs through the dialysis membrane which concluded with satisfactory, sustained and constant drug release phenomenon in the acidic environment reflecting an idea of the fact its favorable release in acidic pH. It is a fact known that dengue fever causes systemic acidosis i.e., reduction of the pH of blood below 7. Thus, when used in-vivo there is a possibility that the formulation has easy access to its target and, performs the desired mechanism of action and can be used as a therapeutic delivery system against the Dengue virus.

Synergistic photocatalytic and antibacterial efficacies of Ag/rGO and Ag/Ag2O/rGO nanocomposites: The impact of oxide formation and ternary heterojunction

This study explores the efficient solvothermal integration of Ag/rGO and Ag/Ag2O/rGO nanocomposites using various solvents and employs thorough characterization procedures. The primary focus is on understanding the nuanced effects resulting from the combination of Ag nanoparticles with rGO nanosheets, particularly in the context of Ag2O formation. For a quantitative assessment of their photocatalytic and antibacterial properties, graphene oxide and silver nitrate (AgNO3) were introduced to create Ag/rGO and Ag/Ag2O/rGO nanocomposites. The results demonstrate a significant enhancement in photocatalytic performance for the Ag/Ag2O/rGO nanocomposite, achieving a rapid degradation of 99.99 % of methylene blue dye within 30 minutes under simulated solar light irradiation. This heightened efficiency is attributed to improved separation and transfer efficiency, as well as the substantial generation of photoinduced carriers due to the incorporation of visibly active Ag2O nanoparticles. Moreover, the Ag/Ag2O/rGO nanocomposite exhibits an enhanced antibacterial activity, reflected in Minimum Inhibitory Concentration (MIC) values of 50 μg/ml for Bacillus cereus and 300 μg/ml for Escherichia coli. The nanocomposite's higher antimicrobial activity against Gram-positive bacteria, particularly Bacillus cereus, is underscored, revealing its potential as an effective antibacterial agent. The formation of a ternary heterojunction in the Ag/Ag2O/rGO nanocomposite results in a notable increase in the production of free radicals, enhancing charge carrier migration and separation efficiency. This quantitative evidence emphasizes the versatile nature of the nanocomposite, highlighting its potential across diverse fields through augmented antibacterial and photocatalytic properties.

A Dynamic Mechanical Analysis on the Compatibilization Effect of Two Different Polymer Waste-Based Compatibilizers in the Fifty/Fifty Polypropylene/Polyamide 6 Blend.

This study aims to examine the 50/50 polypropylene/polyamide 6 (iPP/PA6) system molded under confined flow conditions, both in its original state and after being modified by two different interfacial agents. This study provides two main insights. Firstly, it focuses on a polymer blend close to phase inversion. Secondly, it investigates the impact of using two different types of interfacial agents (derived from polymer waste) to enhance the compatibility between iPP and PA6. Dynamic Mechanical Analysis (DMA) has been employed to achieve these objectives. It is important to note that the investigation of the 50/50 iPP/PA6 system is a crucial focus predicted in previous studies, where a series of mechanical properties were evaluated using Box-Wilson design of experiments (DOEs) over the whole compositional range on the iPP/PA6 binary system. Thus, two interfacial modifiers, namely succinic anhydride (SA)-grafted atactic polypropylene with terminal, side, and bridge SA grafts (aPP-SASA) and succinyl-fluoresceine (SF) with bridge succinic anhydride grafting atactic polypropylene (aPP-SFSA), were employed. The authors obtained and characterized these agents. The quantity of these agents used in the blend was identified as a critical coordinate in prior studies conducted by the authors. The processing method used, compression molding under confined conditions, was chosen to minimize any orientation effect on the emerging morphology. All characterization procedures were performed on samples processed by contour machining to retain the blend morphologies as they emerged from the processing stage. Results from WAXS and SAXS synchrotron tests concluded there were no changes in the crystal morphology of the iPP or the PA6 in the blends nor any co-crystallization process throughout the compositional range. These findings, and the long period fits on the PP crystalline phase for the fifty/fifty blends we are discussing, will support the present DMA study. Finally, the efficiency of these interfacial modifiers has been concluded, even in this unfavorable scenario.

FR2 5G Networks for Industrial Scenarios: Experimental Characterization and Beam Management Procedures in Operational Conditions

FR2 5G Networks for Industrial Scenarios: Experimental Characterization and Beam Management Procedures in Operational Conditions

The Impact of Silver Nanoparticles’ Size on Biofilm Eradication

IntroductionEfficient intracanal disinfection is required for a successful regenerative endodontic treatment. Thus, this study aimed to identify the silver nanoparticles’ (NPs) size (AgNPs) with the highest antibiofilm efficacy when mixed with calcium hydroxide [Ca(OH)2] to eradicate an in vitro endodontic biofilm. MethodsThe various sizes of AgNPs and mixtures were characterized by scanning electron microscopy, transmission electron microscopy, and ultraviolet-visible spectroscopy. A total of 168 dentin root segments were prepared, sterilized, and inoculated for 3 weeks with Actinomyces naeslundii and Fusobacterium nucleatum. Samples were randomly allocated to 4 experimental groups (n = 28/group): 2 nm AgNPs + 35% Ca(OH)2, 5 nm AgNPs + 35% Ca(OH)2, 10 nm AgNPs + 35% Ca(OH)2, and 35% Ca(OH)2 alone. Samples exposed to saline and triple antibiotic paste (TAP) acted as negative and positive control groups, respectively. After 1 and 2 weeks, samples were stained with LIVE/DEAD BacLight dye and examined under a confocal laser scanning microscope to determine the proportion of dead bacteria. ResultsThe characterization procedure revealed a spherical NP's structure with minor aggregations. Except for Ca(OH)2 group, all groups had significantly higher antibiofilm efficacy at 2 weeks. Both the 10 nm mixture (99.5%) and TAP (99.2%) exhibited the highest antibiofilm efficacy at 2 weeks and were not significantly different from one another (P > .05). No significant difference was noted between the 2 and 5 nm mixtures at 1 week (81% and 84%) and 2 weeks (89% and 91%). ConclusionThe 10 nm AgNPs (0.02%) + 35% Ca(OH)2 mixture exhibited the highest antibiofilm efficacy at 2 weeks compared to all other mixtures at both observation periods. Interestingly, the 10 nm mixture performed similarly to TAP at 2 weeks. Excluding Ca(OH)2 group, longer application significantly improved the antibiofilm efficacy of all tested medicaments. Clinical RelevanceThe 10 nm AgNPs + 35% Ca(OH)2 mixture revealed promising results as an intracanal medicament in the regenerative endodontic treatment protocol.

Relationship between characteristic values of shear strength parallel to grain and tensile strength perpendicular to grain for tropical woods

Wood, due to its complex anatomy, requires meticulous characterization that imposes several tests to be carried out to evaluate its properties. Normative codes adopt different specimens to this aim. Geometric specificities proposed by NBR 7190-3:2022 and ASTM D143-22 to specimens used for estimating wood strength in tensile perpendicular to grain (ft90) make them difficult to carry out. Thus it is advisable to consider relating ft90 with another mechanical property, for example, the shear strength parallel to the grain (fv0). This paper aims to establish a coefficient relating the characteristic values of ft90 and fv0, for strength classes D40 and D60 (frequently used for structural purposes) of Brazilian Code 7190:3-2022. A further aim, if possible, is to determine a single representative coefficient for both classes. Tests made it possible to obtain those properties for four species from each class, following NBR 7190-3:2022 guidelines. The optimal coefficient was determined using the least squares method (MMQ). Ratios ft90,k/fv0,k were 0.22 and 0.19, for classes D40 and D60, respectively. As these ratios don’t present a significant difference, it is viable to adopt a single relationship for both classes, thus simplifying characterization procedures.

Pyridazine Derivatives: Molecular Docking, ADMET Prediction, and Synthesis for Antihypertensive Activity.

The drug discovery and development domain has witnessed remarkable advancements due to the integration of computational methods, particularly Computer-Aided Drug Design (CADD). Discovering and creating new drugs involves structural modifications to enhance their effectiveness and physical attributes. This frequently includes employing semisynthetic techniques to investigate structure-activity relationships thoroughly. Noticeable progress in molecular biology, computational chemistry, combinatorial chemistry, and highthroughput screening is steering transformative changes in the pharmaceutical industry. High blood pressure or hypertension, a significant health issue, elevates the chances of heart, kidney, and brain complications, among other health concerns. It's a leading cause of untimely mortality globally. Therefore, it is important to search for new antihypertensive compounds that have fewer side effects and higher therapeutic activity. Following molecular docking of the pyridazine derivatives, compounds were subjected to In-silico ADMET analysis. Subsequently, a low molecular weight compound was synthesized. Among the synthesized compounds characterization procedures include TLC, FT-IR, 1HNMR, and LC-MS techniques. Compound 8 exhibited the most favorable molecular docking results with alpha A1 and beta 1 adrenergic receptors. Compounds 3, 5, and 6 fulfilled the essential ADMET criteria. Subsequently, Compounds 3, 4, and 5 underwent additional synthesis and characterization procedures, including TLC, FT-IR, 1H-NMR, and LC-MS techniques. Similar behavior was observed in compounds 6, 8, 10, and 11, all violating Pfizer's 3/75 rules in terms of TPAS. Hydrazinolysis of these b-benzoyl propionic acids produced pyridazine, which was utilized in synthesizing pyridazine derivatives. TLC, FT-IR, 1HNMR, and LCMS have characterized the compounds.

Biomass extract-mediated preparation of magnesium-doped nickel oxide nanoparticles and their in vitro bioactivities assessment

Biomass extract-mediated fabrication of metal oxide nanoparticles received significant attention in material research because it is eco-friendly, cost-effective, and easy to scalability. Here, the fabrication of Boerhavia diffusa leaf extract (BDE)-mediated magnesium-doped nickel oxide nanoparticles (Mg-doped-NiO-BDE) and their in vitro bioactivities are reported. Several characterization procedures are used to characterize the prepared NiO nanoparticles. Field emission scanning electron microscopy and transmission electron microscopy images exhibit that the Mg-doped-NiO-BDE are distorted spherical. The X-ray diffraction showed that the average crystallite sizes of the nanoparticles are around 51–55 nm. X-ray diffraction, X-ray photoelectron spectroscopy, and energy-dispersive X-ray spectroscopy analysis results confirm that the NiO is produced, and Mg-doping has occurred in the adopted reaction condition. The doped NiO NPs exhibit broad-spectrum antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), as well as antifungal action against Aspergillus niger (A. nigar). The antioxidant, anti-inflammatory, and anti-diabetic efficiencies of doped-NiO NPs (concentration = 400 μg/mL) are around 65 %, 89 %, and 87 %, respectively. The Mg-doped-NiO-BDE nanoparticles show around 75 % viability against the human lung cancer cell line (A459) with a concentration of up to 100 μg/mL, while the IC50 value is 185.6 μg/mL. The scratch test is executed with the mouse fibroblast cells (L929) in which doped-NiO NPs exhibit around 36 % wound-closing ability. The above results signify that the fabricated Mg-doped-NiO-BDE is a potent bioactive material that could be used for biomedical applications.