The effect of reinforcing concrete members with high strength steel bars with yield strength up to 600 MPa on the overall seismic behavior of concrete moment frames was studied experimentally and numerically. Three geometrically identical plane frame models with two bays and two stories, where one frame model was reinforced with hot rolled bars (HRB) with a nominal yield strength of 335 MPa and the other two by high strength steel bars with a nominal yield strength of 600 MPa, were tested under simulated earthquake action considering different axial load ratios to investigate the hysteretic behavior, ductility, strength and stiffness degradation, energy dissipation and plastic deformation characteristics. Test results indicate that utilizing high strength reinforcement can improve the structural resilience, reduce residual deformation and achieve favorable distribution pattern of plastic hinges on beams and columns. The frame models reinforced with normal and high strength steel bars have comparable overall deformation capacity. Compared with the frame model subjected to a low axial load ratio, the ones under a higher axial load ratio exhibit more plump hysteretic loops. The proved reliable finite element analysis software DIANA was used for the numerical simulation of the tests. The analytical results agree well with the experimental results.