Channel Diameter Research Articles

A microwave discharge in high-velocity flows initiated by a half-wave antenna

A microwave discharge in high-velocity (150—250 m/s) air flows induced on a half-wave vibrator is studied. A cw magnetron microwave generator with a frequency of 2.45 GHz and an output power of up to 5 kW was used for initiation of the microwave discharge. The high-speed video imaging was used for studying the discharge structure, determining the diameter and length of the plasma channel as a function of flow velocity and pressure. Electron concentration and temperature, along with characteristic gas temperature, were determined based on the optical spectra. The possibility of using this microwave discharge for ignition of hydrocarbon–air mixtures in combustion chambers of ramjet engines is proved experimentally.

Dynamic characteristics analysis of shock absorber based on fluid simulation

Pneumatic spring dampers are extensively utilized within hydraulic systems, and the flow characteristics of the internal oil play a crucial role in determining noise and vibration levels. To validate the mechanical structure's reliability, the shock absorber was simplified and the fluid domain was extracted using a CFD method. The velocity field and pressure field under different conditions were then simulated and analyzed. A finite element model of the flow field was established, and its accuracy was verified by comparing simulation results of gas side pressure with experimental results. According to the working principle of the oil-gas spring, dynamic active surfaces in contact with the main piston and dynamic driven surfaces in contact with the floating piston were defined, determining moving conditions for dynamic grids. The results indicate that as the diameter of flow channels increases, there is a decrease in average pressure drop within the oil chamber (i.e., pressure loss within the flow field). Additionally, as bend angle increases, average pressure drop decreases. However, optimization effects become less significant beyond a certain bend angle.

Characterizing Theta-Emitter Generation for Use in Microdroplet Reactions.

Theta emitters are useful for generating microdroplets for rapid-mixing reactions. Theta emitters are glass tips containing an internal septum that separates two channels. When used for mixing, the solutions from each channel are sprayed with mixing occurring during electrospray ionization (ESI) with reaction times on the order of microseconds to milliseconds. Theta emitters of increasing size cause the formation of ESI droplets of increasing size, which require longer times for desolvation and increase droplet lifetimes. Droplets with longer lifetimes provide more time for mixing and allow for increased reaction times prior to desolvation. Because theta emitters are typically produced in-house, there is a need to consistently pull tips with a variety of sizes. Herein, we characterize the effect of pull parameters on the generation of distinct-sized theta emitters using a P-1000 tip puller. Of the examined parameters, the velocity value had the largest impact on the channel diameter. This work also compares the effect of pulling parameters between single-channel and theta capillaries to examine how the internal septum in theta capillaries affects tip pulling. We demonstrate the utility of using theta emitters with different sizes for establishing distinct reaction times. Finally, we offer suggestions on producing theta emitters of various sizes while maintaining high repeatability. Through this work, we provide resources to establish a versatile and inexpensive rapid-mixing system for probing biologically relevant systems and performing rapid derivatizations.

Distributed optical fiber sensors for real-time tracking of fouling buildup for tubular continuous polymerization reactors

Early online fouling detection is expected to be a real step forward in the operation of continuous tubular reactors. As an online technology, the Rayleigh backscatter based Distributed Optical Fiber Sensor (DOFS) technology was evaluated with respect to temperature measurement resolution, reproducibility and the best online calibration method. Different coatings and terminations were characterized for emulsion polymerization reactors. Commercially available sensors with acrylate primary coatings, dual acrylate coatings, and polyimide coatings were all compared with each other. It is shown that the presence of a secondary coating significantly alters sensor behaviour. Sensors with only a primary coating showed a temperature resolution of 0.1 °C. Online calibration for temperature readout was carried out and validation tested on segments of fiber integrated in a 3D-printed reactor channel (diameter 1.5 mm). Acrylate coated sensors showed a deviation of 20 % for the calibration coefficients when inside the reactor channel compared to the online calibration, leading to errors in temperature measurement. Polyimide coated sensors showed a deviation of just 0.6 % in this validation test, demonstrating good capabilities for online calibration with accurate temperature measurements. The possibility of spatial and temporal monitoring of fouling buildup through a heat exchanging wall equipped with DOFS was evaluated.

Comparative Study of Thermal Criteria and Fluid-Flow Criteria in Micro and Mini Channel Design Constrains with or without Insert Made of Aluminum Material

Through experimental and simulation results, a comparison between mini and micro channels with inserts or without inserts is conducted; in this case, water is the only working fluid used for present experimentation. In order to research the fluid flow and micro channel features, the results of fluid flowing inside the mini channel and micro channel with insert or without insert separately were admitted. Experiments were performed for various operating and design parameters under counter-flow conditions, with hot water moving into a concentric tube with a 30 mm diameter at 0.00078 kg/sec flow rate. The fluid is moving separately through mini channels with a 2 mm diameter and micro channels with lower than 1 mm dia. at a varying flow rate 0.00015 kg/sec to 0.0062 kg/sec. The working temperatures for hot water and cold fluid were 323 K and 303K, respectively. According to the results, the suggested design of the micro channel with insert performed better than a conventional mini channel, micro channel without insert and mini channel with inserts due to the better thermal conductivity of the aluminum material, effective diameter (1mm) to length ratio of micro channel in which heat exchanges with minimum volume ratio and the additional exposed area made possible by the rectangular shape of the insert, whereas micro channel with micro insert having rectangular shape showing best results as compared with the other designs of channel due to the rich heat passage and effective pressure drop and temperature. Experimentation and simulation results are coming in positive direction, it is directly observed from the error percentage i.e. 2.41% to 4.29%.

Dynamical stiffening of dsDNA confined and stretched in a nanochannel

The internal dynamics of double-stranded DNA (dsDNA) confined within channels of diameters ranging from the persistence length to twice that length were investigated using fluorescence microscopy. By analysing spatiotemporal intensity fluctuations, we derived the intermediate dynamic structure factor. The structure factor consistently exhibited behaviour characteristic of Rouse dynamics, regardless of the channel diameter. Notably, as the channel diameter decreased, the DNA molecules became increasingly elongated along the channel, leading to shorter relaxation times. These findings indicate a dynamic stiffening effect, where the effective spring constant of a stretched polymer chain increases. We propose that this effect has significant implications for controlling DNA motion in biological and biotechnological applications.

Stereolithography-assisted sodium alginate-collagen hydrogel scaffold with molded internal channels

Fabricating internal vascular networks within a hydrogel scaffold is essential for facilitating the supply of nutrients, oxygen, and metabolism exchange required by the encapsulated cells. The challenges in current hydrogel scaffold fabrication involve the difficulty of building adequate internal channels, poor scaffold geometry precision, and low cell viability caused by the fabrication process and polymer material properties. Stereolithography (SLA) stands out as a 3D printing technique distinguished by its superior production efficiency, advanced precision, and remarkable resolution in crafting intricate custom geometries. These attributes establish it as an innovative approach for templates in scaffold fabrication, potentially surpassing the fused deposition modeling (FDM)-based template strategy. Meanwhile, it exerts less shear stress on the cells compared to the direct bioprinting process. This novel strategy enables the fabrication of hydrogel vascular structure within the precision of 500 µm in both channel diameter and wall thickness. In this paper, various sodium alginate and collagen (SA-Col) composite hydrogels with varying collagen concentrations have been investigated to identify the optimal ratio for fabricating hydrogel scaffolds with channels.

Non-negligible buoyancy effect on bubbles travelling in horizontal microchannels of comparable size at small Bond numbers

When a gas bubble is transported by a liquid flow confined within a horizontal channel of comparable size, buoyancy effects are usually assumed to be negligible if the Bond number of the flow is less than unity. However, recent experimental studies showed that buoyancy may still significantly impact the bubble dynamics even when Bo≪1, provided that the flow speed is sufficiently small, such that the viscous and inertial forces are weak. To derive a new criterion to assess the significance of buoyancy on the flow of small bubbles in horizontal microchannels, we have performed systematic numerical simulations using the free software Basilisk, covering a wide range of Bond, capillary and Reynolds numbers, Bo=0.004−0.4, Ca=10−4−0.5 and Re=0−100, and exploring the bubble-to-channel diameter ratios db/D=0.2−0.9. We demonstrate that the nondimensional group Bo(db/D)2/Ca is effective in assessing the importance of buoyancy in flows with negligible inertial effects. When Bo(db/D)2/Ca<0.1, buoyancy effects are negligible and the bubble travels along the channel axis. When Bo(db/D)2/Ca>10, buoyancy effects dominate and the bubble travels in the vicinity of the upper wall. For intermediate values, bubbles take equilibrium positions between the channel centre and the wall, and the threshold Bo(db/D)2/Ca=1 is effective in predicting whether bubbles will travel closer to the channel centre or to the wall. The capillary number at the denominator describes the lift force generated by the deformation of the bubble due to viscous shear, which acts towards the channel centre thus opposing buoyancy. Inertial forces significantly alter the shape and equilibrium position of the bubble when the Weber number of the flow exceeds unity and Bo/We<1. Under the action of inertia, bubbles of size comparable to the channel diameter become elongated but remain centred. Smaller bubbles migrate towards the channel wall and do not exhibit a preferential near-wall circumferential position.

Quantification of elastic modulus variations during zebrafish embryo development using a 3D-printed microfluidic platform

Reliable indicators to assess embryo quality are critical for the in vitro fertilization. Increasing evidence suggests that elasticity is emerging as a potential marker to evaluate the early development of embryos. This paper introduces a 3D-printed microfluidic device to measure the elastic modulus of zebrafish embryos deformed in a circular constriction channel. Firstly, numerical simulation was performed to analyze the impact of inlet pressure, embryo size and constriction channel diameter on the maximum protrusion length of the embryo. Subsequently, the zebrafish embryos were deformed using the device to record the protrusion length which was automatically measured using U-net, before the power-law rheological model was employed to calculate the elastic modulus. Experiments showed the power-law exponent and embryo elasticity were stable as the inlet pressure was not less than 250 mbar. Embryo culturing after squeezes revealed that embryos could maintain normal development even after multiple squeezes at 150 mbar while higher pressure may be fatal. Afterward, the deformation of the yolk was found to increase elasticity by 60.4% compared to cases where only the chorion envelope was deformed. Finally, the elasticity variation of zebrafish embryos was measured for 17hours. It revealed that the elasticity initially increased from hour 1 to hour 7-10 and then returned to approximately the original value during culture from the cleavage to the segmentation stages. The system with the ability of precise and long-term assessment of embryo elasticity may find valuable application potentials in the mechanical evaluation and sorting of embryos.

Does the guide sheath outperform the non-guide sheath method in endobronchial ultrasound-guided biopsy of peripheral pulmonary lesions?-a meta-analysis.

Endobronchial ultrasound (EBUS)-guided transbronchial biopsy with or without a guide sheath (EBUS-GS or EBUS-nGS) is commonly utilized for the diagnosis of peripheral pulmonary lesions (PPLs). The primary objective of this meta-analysis is to assess the diagnostic yield, surgical time, and safety of EBUS-GS and EBUS-nGS in patients presenting with PPLs, providing valuable insights for clinical decision-making. We conducted a systematic search of four databases (PubMed, Embase, Web of Science, Cochrane Library) up to January 2024. Two researchers independently screened the retrieved articles, extracted the data, assessed the quality of the studies, and conducted statistical analysis through Review Manager 5.4 and STATA 14.0. Subgroup analysis was used to explore potential sources of heterogeneity. Publication bias was assessed through funnel plot tests. Sensitivity analyses were also performed to evaluate the robustness of the combined results. The meta-analysis included data from nine studies comprising 2,898 patients. No publication bias was detected. There was no difference in the overall diagnostic rate of EBUS-GS and EBUS-nGS for PPLs [odds ratio (OR): 0.83, 95% confidence interval (CI): 0.64-1.08, Z-score (Z) =1.37, P=0.17]. Conversely, in cases utilizing a bronchoscope with an outer diameter of 3.0 mm (OR: 0.58, 95% CI: 0.40-0.84, Z=2.86, P=0.004), a 1.7-mm bronchoscope channel (OR: 0.70, 95% CI: 0.51-0.96, Z=2.21, P=0.03), or lesions ≤30 mm in size, or lesions situated in the lower lobe of the lung (OR: 0.59, 95% CI: 0.38-0.91, Z=2.36, P=0.02), the diagnostic rate was higher in the EBUS-nGS group. However, the EBUS-GS group demonstrated a tremendous advantage in terms of safety (OR: 0.64, 95% CI: 0.44-0.93, Z=2.33, P=0.02). EBUS-GS and EBUS-nGS showed no significant difference in the overall diagnostic rate for PPLs. When using a bronchoscope with an outer diameter of 3.0 mm or a channel diameter of 1.7 mm, or when lesions are ≤30 mm or located in the lower lobe of the lung, EBUS-nGS demonstrated a higher diagnostic rate, and EBUS-nGS demonstrated a higher diagnostic rate. However, EBUS-GS exhibited more tremendous advantages in terms of safety.

Experimental void fraction measurement approach of small channel two-phase flow

This investigation presents a novel experimental approach for assessing void fraction parameters in two-phase flows within microchannels. The study critiques the conventional impedance methods that utilize alternating current (AC) for void fraction measurements, highlighting the inherent drawbacks such as ground capacitance interference and non-uniform current distribution. To circumvent these issues, the research introduces the use of direct current (DC), which ensures a homogeneous distribution across the conductor's cross-section, as a more reliable alternative. Termed the "Direct Impedance Method," this technique employs DC to enhance the accuracy of void fraction measurements. The study explores various geometric configurations of full-ring and half-ring electrodes, both vertical and horizontal, within a microchannel of 500 μm diameter. An empirical formula is derived to calculate the void fraction from the electrical data obtained. High-speed imaging at 4000 frames per second supplements the method by providing visual confirmation of the flow patterns. The comparative analysis of the direct impedance method and image processing using the homogeneous theoretical model reveals a deviation of approximately 2 %–10 % for the slug and annular flow pattern. In contrast, the deviation is more for the bubble pattern. The results affirm that the Direct Impedance Method is a cost-effective and reasonably precise technique for small-channel applications. Its accuracy is inversely proportional to the channel diameter, rendering it unsuitable for channels larger than 2 mm. The method's low construction cost further enhances its practical appeal.

New ecological knowledge of the bubblegum coral Paragorgia arborea (L. 1758) using computer vision

IntroductionModern stationary observatories, equipped with cameras and other sensors, make it possible to observe and study cold-water corals in situ over increasingly long periods of time. In this study we investigate data collected for a bubblegum coral (Paragorgia arborea) with the LoVe (Lofoten Vesteralen) observatory for a period of 117 days, including time series of images, current, water depth and temperature. This kind of multimodal time series data represent a new methodological challenge for marine technology and bioinformatics.MethodsIn this work, we continue on the path to computer-aided ocean data analysis and show how, in addition to polyp activity, morphological parameters can be extracted from image data with a new deep learning-based approach. Using customized visualizations we show how this image-derived data together with the environmental data can be analysed and new interpretations are supported.ResultsOur results indicate that the polyp activity and branch diameter variation followed a semi-cyclic pattern, with the polyps changing from fully retracted to fully expanded faster than the image sampling rate, resulting in an almost binary time series that showed positive correlation with the current strength, which may be related to food supply. The periods of full polyp expansion ranged from 1 to 4 h, with the majority around 1 h. The variation in branch diameter and volume of intra-skeletal channels probably relates to the intra-colonial transport of nutrients and waste products. During a 16 day period in March 2019, the polyps remained retracted for an extended period accompanied by little variation in branch diameter. This behaviour was not related to any of the measured environmental conditions. Further studies are required to determine if this could be linked to biological processes, such as reproduction.

Design of alveolar biomimetic enhanced heat transfer structure for cylindrical lithium battery pack

Aiming to address the issue of thermal runaway in high energy density batteries during high charge and discharge rates, a heat management system for an alveolar-like honeycomb liquid-cooled power battery was designed. This system is inspired by the bionic concept of cell cooling in biological tissues, with cylindrical batteries serving as cells and cooling channels resembling blood vessels. The cooling system for a battery pack consisting of 24 cylindrical cells was designed and implemented. Numerical simulations were conducted to optimize and study the effects of inlet diameter, branch angle, flow rate, and spiral baffle on coolant flow uniformity and temperature distribution within the battery pack. The results indicate that as the inlet diameter of the fractal channel increases from 2 mm to 20 mm, the coolant flow uniformity improves and the pressure drop decreases. However, the influence of inlet diameter diminishes after reaching a certain limit. When the inlet diameter increases from 10 mm to 20 mm, the relative standard deviation of the velocity at the branch outlet decreases only slightly, from 0.9 % to 0.8 %. Increasing the branch angle from 30° to 75° leads to an increase in the relative standard deviation of velocity, from 0.79 % to 1.24 %. Incorporating a spiral baffle into the internal flow channel of honeycomb cooling significantly enhances temperature uniformity within the battery pack while reducing maximum temperature levels considerably. Furthermore, a smaller pitch for the spiral baffle yields better cooling performance for the immersed liquid cooling thermal management system. After optimization, the maximum temperature difference is only 1.27 °C across the surface of the battery pack, achieving a remarkable temperature uniformity level of just 0.11 %.

A novel multi-channel applicator with a U-shaped channel for vaginal intracavity brachytherapy.

Endometrial cancer is one of the most common gynecological malignancies in the world. Vaginal brachytherapy is an important postoperative adjuvant treatment for endometrial cancer. However, a common problem with existing applicators is insufficient dose at the vaginal apex. This study describes the Hangzhou (HZ) cylinder, a novel 3D printed vaginal intracavity brachytherapy applicator, detailing its characteristics, dose distribution, and clinical applications. The HZ cylinder is distinguished by its unique structure: a U-shaped channel with a 2mm diameter, a straight central axis channel of the same diameter, and 10 parallel straight channels. For comparison, standard plans were employed, designed to ensure that a minimum of 95% of the prescribed dose reached 5mm beneath the mucosal surface. We conducted comparative analyses of mucosal surface doses and doses at a 5mm depth below the mucosa between the HZ cylinder and a conventional single-channel cylinder across various treatment schemes. Additionally, the study examined dose differences in target volume and organs at risk (OARs) between actual HZ cylinder plans and hypothetical single-channel plans. In the standard plans, mucosal surface doses at the apex of the vagina were 209.32% and 200.61% of the prescribed dose with the HZ and single-channel cylinders, respectively. The doses on the left and right wall mucosal surfaces varied from 149.26% to 178.13% and 142.98% to 180.75% of the prescribed dose, and on the anterior and posterior wall mucosal surfaces varied from 128.87% to 138.50% and 142.98% to 180.75% of the prescribed dose. Analysis of 24 actual treatment plans revealed that when the vaginal tissue volume dose covering 98% (vaginal D98%) was comparable between the HZ cylinder and virtual single-channel plans (6.74±0.07Gy vs. 6.69±0.10Gy, p=0.24), rectum doses of HZ cylinder plans were significantly lower than those of single-channel plans (D1cc, 5.96±0.56Gy vs. 6.26±0.71Gy, p=0.02 and D2cc, 5.26±0.52Gy vs. 5.56±0.62Gy, p=0.02). The HZ cylinder demonstrates a reduction in dose to the rectum and bladder while maintaining adequate target volume coverage. Its mucosal surface dose is comparable to that of the traditional single-channel cylinder. These findings suggest that the HZ cylinder is a viable and potentially safer alternative for vaginal brachytherapy, warranting further investigation with larger sample sizes.

Using the space-time evolution spectrum to investigate the transmission characteristics of lightning return stroke current

Exploring the physical characteristics of lightning discharge channels based on spectrum can help to reveal the microscopic mechanism of the discharge process and provide a theoretical basis for lightning protection research. According to the spectra of six cloud-to-ground lightning return stroke processes and plasma conduction theory, we investigated the evolution of the total intensity of ionic and atomic lines in the spectra, conductivity, and other parameters along the discharge channel at different stages of the return stroke. The results show that the intensity of ionic lines in the spectrum present an obvious decaying trend with increasing height along the channel during the initial current-rise phase of the return stroke. In the later stages, the decay of ionic line intensity along the channel height slowed down. The intensity of the atomic lines varied slightly with increasing height along the channel during the entire return stroke process. The different space-time evolution characteristics of the spectral line intensity with different excitation energies reflect the differences in the mechanism of light radiation in the core current-carrying and surrounding thermal channels. The spectral characteristics further confirm that the ionic line with higher excitation energy directly relates to the physical process in the core current-carrying channel, and its intensity can roughly reflect the change in current along the channel. The attenuation of the return stroke current along the channel was mainly manifested in the initial stage of the return stroke. The decay rate of conductivity along the channel height is slower than that of the ionic line intensity throughout the return stroke stage, which is related to the channel diameter, it is another key parameter affecting the current transmission in the discharge channel.

Thermal Analysis of Micro-Channel Internal Cooling in Cutting Tools: A Machine Learning Approach

The use of coolants for cutting process in metal cutting operations is customary. Turning causes high cutting heat in nickel base super alloy Inconel 718. Nonetheless, it should be acknowledged that although flooding techniques are commonly used in the machining of super alloys, these flood cooling methods have extremely poor efficiencies. Another alternative to increase the cooling capabilities of fluids would be an internal-cooling approach that would enable to lower machining temperatures significantly. The heat dissipation ability in the tool is also greatly influenced by the micro-channel diameter of tool which further causes a significant effect on the coolant outlet velocity. A design of an internal-cooling single point cutting tool with micro channel structures for enhanced coolant heat transfer capability and reduced machining temperature is used for turning Inconel 718 under dry, flooded cooling and internal cooling to study the effects of cooling conditions on cutting force, cutting temperature and surface quality. A regression model is built using the Random Forest (RF) and Support Vector Regression (SVR) methods in machine learning framework. These models were then used to forecast input parameters, such as channel diameter and inlet pressure, which made it easier to obtain output data, such as pressure and maximum velocities at different notches. Eighty percent of the data in the dataset is used to train the model and with the remaining twenty percent set aside for evaluating the model's functionality. When comparing internal-cooling technology to traditional flood cooling, there are clear benefits including increased heat transfer efficiency, which leads to lower cutting temperatures, less cutting force, and better surface quality. More specifically, in the internal-cooling configuration, a direct relationship is shown between rising coolant inlet pressure and falling cutting force and temperature over time. Further highlighting the advantages of this cooling strategy is the relationship between increased intake pressure and decreased surface roughness.

A Navier-slip model for liquid film thickness in gas–liquid slug flow in capillaries

ABSTRACT The motion of a long gas bubble in a continuous liquid phase in a small diameter channel results in a thin liquid film between the bubble and the channel wall. Generally, a free-slip (zero shear) boundary condition is applied at the gas–liquid interphase boundary which is applicable only at low gas velocities. However, at increased flow rates of gas phase, shear stress at the gas–liquid interface is non-negligible. Applying non-zero shear at the interface requires knowledge of velocity profile in the gas phase. In this work, we circumvent this problem by using a slip length like parameter to take into account the velocity gradient in the gas phase. The slip length is infinite for the free-slip condition at the interface and zero for the no-slip condition. An expression is derived for the film thickness in terms of slip length and capillary number and is compared with the experimental data and other correlations available in the literature.

A Semiflexible Polymer Translocation Through a Cylindrical Channel

In this study, translocation of a semi flexible polymer through a cylindrical channel have been investigated. A two-dimensional Monte Carlo simulation was employed, by utilizing the bond fluctuation method (BFM) to investigate the translocation processes of a chain length N. To surmount the entropic barrier, the middle monomers of the polymer have been positioned at the center of the pore, which is situated between the CIS and TRANS regions. Consequently, the static properties of a semi-flexible polymer by calculating the mean square end-to-end distance ‹R2› and the mean square radius of gyration ‹R&amp;lt;sup&amp;gt;g&amp;lt;/sup&amp;gt;2› as functions of the chain length (N) have been examined. The mean square end-to-end distance and the mean square radius of gyration are proportional to the number of monomers N as ‹R2› ~ N1.496 and ‹R2g› ~ N1.505 correspondingly for a short cylindrical channel length L = 2, which aligns with the theoretically predicted. These finding indicates that the relationships between ‹R2› and ‹R&amp;lt;sup&amp;gt;g&amp;lt;/sup&amp;gt;2› and the polymer chain size N are strongly influenced by the channel length L. The dynamic properties by analyzing the translocation time of the polymers also studied. Additionally, the relationship between the escape time τ and the polymer chain length N depends on the pore width W, which is equivalent to the diameter of the cylindrical channel. These research demonstrates that the escape time τ decreases as the width increases and escape time τ increases as the chain stiffness increases.

Effect of flow patterns on recovery of invertases from Saccharomyces cerevisiae using polyethylene glycol/magnesium sulfate system in microchannels

The use of microdevices in extraction processes in aqueous two-phase systems (ATPS) has been studied for years in the literature. However, the effects of flow patterns still need to be adequately explored, especially for real samples. In this sense, the present study investigated the performance of microdevices in the recovery and partial purification of invertases with varying flow patterns. The invertases from Saccharomyces cerevisiae and the polyethylene glycol 1500/magnesium sulfate system were used in the experiments. Beyond the flow pattern, the study sought to maximize recovery and purification factor (PF) values by varying other operating conditions, including residence time, volumetric ratio, and channel diameter. It was observed that the short slug and long slug patterns showed better separation performance than the parallel pattern regardless of residence time and volumetric ratio. Increasing the residence time from 15 to 35 min increased the top recovery and PF values by two times, indicating that S. cerevisiae invertase partition is slow to the top phase. The maximum values of recovery (57.01 % ± 2.67 %) and PF (4.16 ± 0.06) were reached in the condition with long slug pattern, 35 min of operation, volumetric ratio of 2:1, and a channel diameter of 0.51 mm. The present study provides valuable information for integrating microdevices in a protocol for purifying invertase from S. cerevisiae and other proteins in real systems.

Numerical simulation research on the launch process of pneumatic launch device

Abstract In order to deal with the potential threats posed by low-altitude UAVs, an anti-UAV net-catching device was designed based on the principle of pneumatic launch. The internal ballistic calculation model of the net-catching device during the launch process was established, and the pressure-stabilizing air chamber of the device was numerically simulated. The impact of pressure, air chamber volume, gas channel diameter and barrel length on the performance of the launch device to capture the UAV was analyzed. The internal relationship between different influencing factors on the muzzle velocity and the air pressure drop of a single shot was analyzed. Selecting appropriate internal ballistic characteristics to capture UAVs at different target distances provides theoretical guidance for in-depth research on anti-UAV netting devices.